P21761 (TRFR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thyrotropin-releasing hormone receptor
Short name=TRH-R

Alternative name(s):
Thyroliberin receptor

Gene names

Name:

Trhr

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for thyrotropin-releasing hormone. This receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thyrotropin-releasing hormone receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Thyrotropin-releasing hormone receptor		PRO_0000070188
Regions
	Topological domain	1 – 28	28	Extracellular Potential
	Transmembrane	29 – 51	23	Helical; Name=1; Potential
	Topological domain	52 – 61	10	Cytoplasmic Potential
	Transmembrane	62 – 83	22	Helical; Name=2; Potential
	Topological domain	84 – 99	16	Extracellular Potential
	Transmembrane	100 – 121	22	Helical; Name=3; Potential
	Topological domain	122 – 144	23	Cytoplasmic Potential
	Transmembrane	145 – 168	24	Helical; Name=4; Potential
	Topological domain	169 – 193	25	Extracellular Potential
	Transmembrane	194 – 215	22	Helical; Name=5; Potential
	Topological domain	216 – 266	51	Cytoplasmic Potential
	Transmembrane	267 – 288	22	Helical; Name=6; Potential
	Topological domain	289 – 296	8	Extracellular Potential
	Transmembrane	297 – 319	23	Helical; Name=7; Potential
	Topological domain	320 – 393	74	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	3	1	N-linked (GlcNAc...) Potential
	Glycosylation	10	1	N-linked (GlcNAc...) Potential
	Disulfide bond	98 ↔ 179		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21761 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 8739B75D1A0FCCFC	FASTA	393	44,559
	10 20 30 40 50 60 MENDTVSEMN QTELQPQAAV ALEYQVVTIL LVVIICGLGI VGNIMVVLVV MRTKHMRTPT 70 80 90 100 110 120 NCYLVSLAVA DLMVLVAAGL PNITDSIYGS WVYGYVGCLC ITYLQYLGIN ASSCSITAFT 130 140 150 160 170 180 IERYIAICHP IKAQFLCTFS RAKKIIIFVW AFTSIYCMLW FFLLDLNIST YKNAVVVSCG 190 200 210 220 230 240 YKISRNYYSP IYLMDFGVFY VVPMILATVL YGFIARILFL NPIPSDPKEN SKMWKNDSIH 250 260 270 280 290 300 QNKNLNLNAT NRCFNSTVSS RKQVTKMLAV VVILFALLWM PYRTLVVVNS FLSSPFQENW 310 320 330 340 350 360 FLLFCRICIY LNSAINPVIY NLMSQKFRAA FRKLCNCKQK PTEKAANYSV ALNYSVIKES 370 380 390 DRFSTELEDI TVTDTYVSTT KVSFDDTCLA SEN

References

[1]	"Expression cloning of a cDNA encoding the mouse pituitary thyrotropin-releasing hormone receptor." Straub R.E., Frech G.C., Joho R.H., Gershengorn M.C. Proc. Natl. Acad. Sci. U.S.A. 87:9514-9518(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pituitary.
[2]	"Regulation by thyrotropin-releasing hormone (TRH) of TRH receptor mRNA degradation in rat pituitary GH3 cells." Narayanan C.S., Fujimoto J., Geras-Raaka E., Gershengorn M.C. J. Biol. Chem. 267:17296-17303(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"An alternative splice variant of the mouse TRH receptor mRNA is the major form expressed in the mouse pituitary gland." Jones K.E., Brubaker J.H., Chin W.W. J. Mol. Endocrinol. 16:197-204(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 332-393. Tissue: Pituitary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M59811 mRNA. Translation: AAA40480.1. M94384 mRNA. Translation: AAA40437.1. L48936 mRNA. Translation: AAA81559.1.
IPI	IPI00127651.
PIR	A39251.
RefSeq	NP_038724.1. NM_013696.2.
UniGene	Mm.309350.
3D structure databases
ProteinModelPortal	P21761.
SMR	P21761. Positions 26-346.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000105918.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P21761.
Proteomic databases
PRIDE	P21761.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000038856; ENSMUSP00000036320; ENSMUSG00000038760. ENSMUST00000110289; ENSMUSP00000105918; ENSMUSG00000038760.
GeneID	22045.
KEGG	mmu:22045.
Organism-specific databases
CTD	7201.
MGI	MGI:98824. Trhr.
Phylogenomic databases
eggNOG	NOG303917.
HOGENOM	HOG000017249.
HOVERGEN	HBG007549.
InParanoid	P21761.
KO	K04282.
OMA	PTEKPAN.
OrthoDB	EOG4XKV75.
Gene expression databases
ArrayExpress	P21761.
Bgee	P21761.
CleanEx	MM_TRHR.
Genevestigator	P21761.
GermOnline	ENSMUSG00000038760. Mus musculus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR009144. Thyrotropin_RHR. IPR002120. Trelhrm_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00751. THYROLIBRINR. PR01846. TRHRFAMILY.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P21761.
ChEMBL	CHEMBL2467.
NextBio	301836.
SOURCE	Search...

Entry information

Entry name

TRFR_MOUSE

Accession

Primary (citable) accession number: P21761

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program