Reviewed, UniProtKB/Swiss-Prot P21757 (MSRE_HUMAN)

Names and origin

Protein names

Recommended name:
    Macrophage scavenger receptor types I and II
Alternative name(s):
    Macrophage acetylated LDL receptor I and II
    Scavenger receptor class A member 1
    CD_antigen=CD204

Gene names

Name:	MSR1
Synonyms:	SCARA1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). Isoform III does not internalize actetylated LDL.
Subunit structure	Homotrimer.
Subcellular location	Membrane; Single-pass type II membrane protein.
Tissue specificity	Isoform I, isoform II and isoform III are expressed in monocyte-derived macrophages. Ref.2
Sequence similarities	Contains 1 collagen-like domain. Contains 1 SRCR domain.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	LDL Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Collagen Signal-anchor Transmembrane
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	cholesterol transport Inferred from sequence or structural similarity. Source: UniProtKB lipoprotein particle clearance Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of foam cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB receptor-mediated endocytosis Ref.1 Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.7 Traceable author statement. Source: ProtInc low-density lipoprotein particle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	low-density lipoprotein binding Inferred from sequence or structural similarity. Source: UniProtKB scavenger receptor activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HOOK3	Q86VS8	2	EBI-1776976,EBI-1777078
Hook3	Q7TQ77	1	EBI-1776976,EBI-1777000	From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform I (identifier: P21757-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform II (identifier: P21757-2)

The sequence of this isoform differs from the canonical sequence as follows:
     345-358: TPFTKVRLVGGSGP → RPVQLTDHIRAGPS
     359-451: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform III (identifier: P21757-3)

The sequence of this isoform differs from the canonical sequence as follows:
     345-408: TPFTKVRLVGGSGPHEGRVEILHSGQWGTICDDRWEVRVGQVVCRSLGYPGVQAVHKAAHFGQG → S

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	Macrophage scavenger receptor types I and II		PRO_0000181627
Regions
	Topological domain	1 – 50	50	Cytoplasmic Potential
	Transmembrane	51 – 76	26	Signal-anchor for type II membrane protein Potential
	Topological domain	77 – 451	375	Extracellular Potential
	Domain	273 – 341	69	Collagen-like
	Domain	350 – 450	101	SRCR
	Region	77 – 109	33	Spacer Probable
	Coiled coil	171 – 255	85	Potential
Amino acid modifications
	Modified residue	27	1	Phosphoserine By similarity
	Modified residue	29	1	Phosphothreonine By similarity
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	102	1	N-linked (GlcNAc...) Potential
	Glycosylation	143	1	N-linked (GlcNAc...) Potential
	Glycosylation	184	1	N-linked (GlcNAc...) Potential
	Glycosylation	221	1	N-linked (GlcNAc...)
	Glycosylation	249	1	N-linked (GlcNAc...) Potential
	Glycosylation	267	1	N-linked (GlcNAc...) Potential
	Disulfide bond	375 ↔ 439		Ref.8
	Disulfide bond	388 ↔ 449		Ref.8
	Disulfide bond	419 ↔ 429		Ref.8
Natural variations
	Alternative sequence	345 – 408	64	TPFTK…HFGQG → S in isoform III.		VSP_036842
	Alternative sequence	345 – 358	14	TPFTK…GGSGP → RPVQLTDHIRAGPS in isoform II.		VSP_006229
	Alternative sequence	359 – 451	93	Missing in isoform II.		VSP_006230
	Natural variant	23	1	F → C Ref.3		VAR_025190
	Natural variant	269	1	T → I: dbSNP rs13306543.		VAR_052061
	Natural variant	275	1	P → A: dbSNP rs3747531. Ref.3		VAR_025191

Sequences

	Sequence		Length	Mass (Da)
	Isoform I [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 915C03B56653BB41	FASTA	451	49,762
	10 20 30 40 50 60 MEQWDHFHNQ QEDTDSCSES VKFDARSMTA LLPPNPKNSP SLQEKLKSFK AALIALYLLV 70 80 90 100 110 120 FAVLIPLIGI VAAQLLKWET KNCSVSSTNA NDITQSLTGK GNDSEEEMRF QEVFMEHMSN 130 140 150 160 170 180 MEKRIQHILD MEANLMDTEH FQNFSMTTDQ RFNDILLQLS TLFSSVQGHG NAIDEISKSL 190 200 210 220 230 240 ISLNTTLLDL QLNIENLNGK IQENTFKQQE EISKLEERVY NVSAEIMAMK EEQVHLEQEI 250 260 270 280 290 300 KGEVKVLNNI TNDLRLKDWE HSQTLRNITL IQGPPGPPGE KGDRGPTGES GPRGFPGPIG 310 320 330 340 350 360 PPGLKGDRGA IGFPGSRGLP GYAGRPGNSG PKGQKGEKGS GNTLTPFTKV RLVGGSGPHE 370 380 390 400 410 420 GRVEILHSGQ WGTICDDRWE VRVGQVVCRS LGYPGVQAVH KAAHFGQGTG PIWLNEVFCF 430 440 450 GRESSIEECK IRQWGTRACS HSEDAGVTCT L
	Isoform II. Checksum: 5F8593EF21189917	FASTA	358	39,584
	10 20 30 40 50 60 MEQWDHFHNQ QEDTDSCSES VKFDARSMTA LLPPNPKNSP SLQEKLKSFK AALIALYLLV 70 80 90 100 110 120 FAVLIPLIGI VAAQLLKWET KNCSVSSTNA NDITQSLTGK GNDSEEEMRF QEVFMEHMSN 130 140 150 160 170 180 MEKRIQHILD MEANLMDTEH FQNFSMTTDQ RFNDILLQLS TLFSSVQGHG NAIDEISKSL 190 200 210 220 230 240 ISLNTTLLDL QLNIENLNGK IQENTFKQQE EISKLEERVY NVSAEIMAMK EEQVHLEQEI 250 260 270 280 290 300 KGEVKVLNNI TNDLRLKDWE HSQTLRNITL IQGPPGPPGE KGDRGPTGES GPRGFPGPIG 310 320 330 340 350 PPGLKGDRGA IGFPGSRGLP GYAGRPGNSG PKGQKGEKGS GNTLRPVQLT DHIRAGPS
	Isoform III. Checksum: D6A20BCE57BFBC46	FASTA	388	42,942
	10 20 30 40 50 60 MEQWDHFHNQ QEDTDSCSES VKFDARSMTA LLPPNPKNSP SLQEKLKSFK AALIALYLLV 70 80 90 100 110 120 FAVLIPLIGI VAAQLLKWET KNCSVSSTNA NDITQSLTGK GNDSEEEMRF QEVFMEHMSN 130 140 150 160 170 180 MEKRIQHILD MEANLMDTEH FQNFSMTTDQ RFNDILLQLS TLFSSVQGHG NAIDEISKSL 190 200 210 220 230 240 ISLNTTLLDL QLNIENLNGK IQENTFKQQE EISKLEERVY NVSAEIMAMK EEQVHLEQEI 250 260 270 280 290 300 KGEVKVLNNI TNDLRLKDWE HSQTLRNITL IQGPPGPPGE KGDRGPTGES GPRGFPGPIG 310 320 330 340 350 360 PPGLKGDRGA IGFPGSRGLP GYAGRPGNSG PKGQKGEKGS GNTLSTGPIW LNEVFCFGRE 370 380 SSIEECKIRQ WGTRACSHSE DAGVTCTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human macrophage scavenger receptors: primary structure, expression, and localization in atherosclerotic lesions." Matsumoto A., Naito M., Itakura H., Ikemoto S., Asaoka H., Hayakawa I., Kanamori H., Aburatani H., Takaku F., Suzuki H., Kobari Y., Miyai T., Takahashi K., Cohen E.H., Wydro R., Housman D.E., Kodama T. Proc. Natl. Acad. Sci. U.S.A. 87:9133-9137(1990) [PubMed: 2251254] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS I AND II).
[2]	"A naturally occurring isoform of the human macrophage scavenger receptor (SR-A) gene generated by alternative splicing blocks modified LDL uptake." Gough P.J., Greaves D.R., Gordon S. J. Lipid Res. 39:531-543(1998) [PubMed: 9548586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM III), TISSUE SPECIFICITY.
[3]	NIEHS SNPs program Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS CYS-23 AND ALA-275.
[4]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed: 16421571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM I). Tissue: Testis.
[7]	"Structure, organization, and chromosomal mapping of the human macrophage scavenger receptor gene." Emi M., Asaoka H., Matsumoto A., Itakura H., Kurihara Y., Wada Y., Kanamori H., Yazaki Y., Takahashi E., Lepert M. J. Biol. Chem. 268:2120-2125(1993) [PubMed: 8093617] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 155-272.
[8]	"Structures of class A macrophage scavenger receptors. Electron microscopic study of flexible, multidomain, fibrous proteins and determination of the disulfide bond pattern of the scavenger receptor cysteine-rich domain." Resnick D., Chatterton J.E., Schwartz K., Slayter H., Krieger M. J. Biol. Chem. 271:26924-26930(1996) [PubMed: 8900177] [Abstract] Cited for: DISULFIDE BONDS IN SRCR DOMAIN.
[9]	"An unappreciated role for RNA surveillance." Hillman R.T., Green R.E., Brenner S.E. Genome Biol. 5:RESEARCH008.1-RESEARCH008.16(2004) [PubMed: 14759258] [Abstract] Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
[10]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-221, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBLGenBankDDBJ	D90187 mRNA. Translation: BAA14208.1. D90188 mRNA. Translation: BAA14209.1. AF037351 mRNA. Translation: AAC09251.1. DQ144993 Genomic DNA. Translation: AAZ38715.1. AC023396 Genomic DNA. No translation available. CH471080 Genomic DNA. Translation: EAW63832.1. BC063878 mRNA. Translation: AAH63878.1. D13263 Genomic DNA. No translation available.
IPI	IPI00010351. IPI00012740. IPI00071002.
PIR	A38415. B38415.
RefSeq	NP_002436.1. NP_619729.1. NP_619730.1.
UniGene	Hs.147635
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	P21757.
Proteomic databases
PRIDE	P21757.
Genome annotation databases
Ensembl	ENSG00000038945. Homo sapiens. [Contig view]
GeneID	4481.
KEGG	hsa:4481.
Organism-specific databases
GeneCards	GC08M016009.
H-InvDB	HIX0034246.
HGNC	HGNC:7376. MSR1.
HPA	HPA000272.
MIM	153622. gene.
PharmGKB	PA31181.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P21757.
HOVERGEN	P21757.
OMA	P21757. EVKVLNN.
Gene expression databases
ArrayExpress	P21757.
Bgee	P21757.
CleanEx	HS_MSR1.
GermOnline	ENSG00000038945. Homo sapiens.
Family and domain databases
InterPro	IPR008160. Collagen. IPR003543. Macro_scav_rcpt. IPR001190. Srcr_rcpt. IPR017448. Srcr_rcpt-rel. [Graphical view]
Pfam	PF01391. Collagen. 1 hit. PF03523. Macscav_rec. 1 hit. PF00530. SRCR. 1 hit. [Graphical view]
PRINTS	PR01408. MACSCAVRCPTR.
ProDom	PD000007. Clg_helix. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00202. SR. 1 hit. [Graphical view]
PROSITE	PS00420. SRCR_1. 1 hit. PS50287. SRCR_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	17337.
SOURCE	Search...

Entry information

Entry name

MSRE_HUMAN

Accession

Primary (citable) accession number: P21757
Secondary accession number(s): O60505, P21759, Q45F10

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	June 16, 2009
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)