Reviewed, UniProtKB/Swiss-Prot P21754 (ZP3_HUMAN)

Names and origin

Protein names

Recommended name:
    Zona pellucida sperm-binding protein 3
Alternative name(s):
    Zona pellucida glycoprotein ZP3
      Short name=Zona pellucida protein C
    Sperm receptor
    ZP3A/ZP3B
Cleaved into the following 1 chains:
    1- Recommended name:
            Processed zona pellucida sperm-binding protein 3

Gene names

Name:	ZP3
Synonyms:	ZP3A, ZP3B, ZPC

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.
Subunit structure	Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers By similarity.
Subcellular location	Processed zona pellucida sperm-binding protein 3: Secreted › extracellular space › extracellular matrix. Cell membrane; Single-pass type I membrane protein.
Tissue specificity	Oocytes.
Domain	The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
Post-translational modification	Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida. N-glycosylated By similarity. O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy By similarity.
Sequence similarities	Belongs to the ZP domain family. ZPC subfamily. Contains 1 ZP domain.

Ontologies

Keywords
Biological process	Fertilization
Cellular component	Cell membrane Extracellular matrix Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane
Molecular function	Receptor
PTM	Cleavage on pair of basic residues Glycoprotein Pyrrolidone carboxylic acid
Gene Ontology (GO)
Molecular function	acrosin binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform ZP3A (identifier: P21754-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform ZP3B (identifier: P21754-2)

The sequence of this isoform differs from the canonical sequence as follows:
     364-372: PLIFLDRRG → ATDLPGQEW
     373-424: Missing.

Notes: Found in a second polymorphic locus which, due to an extra G residue in exon 8, has the potential to encode a truncated protein of 372 amino acids.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 350	328	Zona pellucida sperm-binding protein 3
	Chain	23 – ?		Processed zona pellucida sperm-binding protein 3
	Propeptide	351 – 424	74	Removed in mature form By similarity
Regions
	Topological domain	23 – 387	365	Extracellular Potential
	Transmembrane	388 – 408	21	Potential
	Topological domain	409 – 424	16	Cytoplasmic Potential
	Domain	45 – 307	263	ZP
Amino acid modifications
	Modified residue	23	1	Pyrrolidone carboxylic acid By similarity
	Glycosylation	125	1	N-linked (GlcNAc...) By similarity
	Glycosylation	147	1	N-linked (GlcNAc...) By similarity
	Glycosylation	156	1	O-linked (GalNAc...) By similarity
	Glycosylation	162	1	O-linked (GalNAc...) By similarity
	Glycosylation	163	1	O-linked (GalNAc...) By similarity
	Glycosylation	226	1	N-linked (GlcNAc...) Potential
	Glycosylation	272	1	N-linked (GlcNAc...) By similarity
	Disulfide bond	78 ↔ 99		By similarity
	Disulfide bond	217 ↔ 282		By similarity
	Disulfide bond	239 ↔ 300		By similarity
Natural variations
	Alternative sequence	364 – 372	9	PLIFLDRRG → ATDLPGQEW in isoform ZP3B.
	Alternative sequence	373 – 424	52	Missing in isoform ZP3B.
Experimental info
	Sequence conflict	345	1	R → T in CAA40095. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform ZP3A [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 94517B7B66EFFE06	FASTA	424	47,029
	10 20 30 40 50 60 MELSYRLFIC LLLWGSTELC YPQPLWLLQG GASHPETSVQ PVLVECQEAT LMVMVSKDLF 70 80 90 100 110 120 GTGKLIRAAD LTLGPEACEP LVSMDTEDVV RFEVGLHECG NSMQVTDDAL VYSTFLLHDP 130 140 150 160 170 180 RPVGNLSIVR TNRAEIPIEC RYPRQGNVSS QAILPTWLPF RTTVFSEEKL TFSLRLMEEN 190 200 210 220 230 240 WNAEKRSPTF HLGDAAHLQA EIHTGSHVPL RLFVDHCVAT PTPDQNASPY HTIVDFHGCL 250 260 270 280 290 300 VDGLTDASSA FKVPRPGPDT LQFTVDVFHF ANDSRNMIYI TCHLKVTLAE QDPDELNKAC 310 320 330 340 350 360 SFSKPSNSWF PVEGPADICQ CCNKGDCGTP SHSRRQPHVM SQWSRSASRN RRHVTEEADV 370 380 390 400 410 420 TVGPLIFLDR RGDHEVEQWA LPSDTSVVLL GVGLAVVVSL TLTAVILVLT RRCRTASHPV SASE
	Isoform ZP3B [UniParc]. Checksum: AB78DB5571D87B4A		372	41,479
	10 20 30 40 50 60 MELSYRLFIC LLLWGSTELC YPQPLWLLQG GASHPETSVQ PVLVECQEAT LMVMVSKDLF 70 80 90 100 110 120 GTGKLIRAAD LTLGPEACEP LVSMDTEDVV RFEVGLHECG NSMQVTDDAL VYSTFLLHDP 130 140 150 160 170 180 RPVGNLSIVR TNRAEIPIEC RYPRQGNVSS QAILPTWLPF RTTVFSEEKL TFSLRLMEEN 190 200 210 220 230 240 WNAEKRSPTF HLGDAAHLQA EIHTGSHVPL RLFVDHCVAT PTPDQNASPY HTIVDFHGCL 250 260 270 280 290 300 VDGLTDASSA FKVPRPGPDT LQFTVDVFHF ANDSRNMIYI TCHLKVTLAE QDPDELNKAC 310 320 330 340 350 360 SFSKPSNSWF PVEGPADICQ CCNKGDCGTP SHSRRQPHVM SQWSRSASRN RRHVTEEADV 370 TVGATDLPGQ EW

References

[1]	"Human homolog of the mouse sperm receptor." Chamberlin M.E., Dean J. Proc. Natl. Acad. Sci. U.S.A. 87:6014-6018(1990) [PubMed: 2385582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ZP3A).
[2]	"Cloning and characterization of the human sperm receptor ligand ZP3: evidence for a second polymorphic allele with a different frequency in the Caucasian and Japanese populations." van Duin M., Polman J.E., Verkoelen C.C., Bunschoten H., Meyerink J.H., Olijve W., Aitken R.J. Genomics 14:1064-1070(1992) [PubMed: 1478648] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ZP3A AND ZP3B). Tissue: Ovary.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Molecular chastity - Issue 93 of April 2008

Cross-references

Sequence databases
EMBLGenBankDDBJ	M60504 mRNA. Translation: AAA61336.1. X56777 mRNA. Translation: CAA40095.1.
PIR	A36000.
RefSeq	NP_001103824.1.
UniGene	Hs.656137
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000188372. Homo sapiens. [Contig view]
GeneID	7784.
KEGG	hsa:7784.
Organism-specific databases
H-InvDB	HIX0023203.
HGNC	HGNC:13189. ZP3.
MIM	182889. gene.
PharmGKB	PA33528.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P21754.
HOVERGEN	P21754.
Gene expression databases
CleanEx	HS_ZP3.
GermOnline	ENSG00000188372. Homo sapiens.
Family and domain databases
InterPro	IPR001507. Endoglin/CD105. [Graphical view]
Pfam	PF00100. Zona_pellucida. 1 hit. [Graphical view]
PRINTS	PR00023. ZPELLUCIDA.
SMART	SM00241. ZP. 1 hit. [Graphical view]
PROSITE	PS00682. ZP_1. 1 hit. PS51034. ZP_2. 1 hit. [Graphical view]
ProDom	P21754. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P21754.
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

ZP3_HUMAN

Accession

Primary (citable) accession number: P21754
Secondary accession number(s): Q06633

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	July 22, 2008
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)