P21744 (IBP4_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-like growth factor-binding protein 4
Short name=IBP-4
Short name=IGF-binding protein 4
Short name=IGFBP-4

Gene names

Name:	Igfbp4
Synonyms:	Igfbp-4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Subunit structure	Binds IGF2 more than IGF1.
Subcellular location	Secreted.
Sequence similarities	Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Growth factor binding
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	inflammatory response Inferred from expression pattern. Source: RGD intracellular signal transduction Traceable author statement. Source: RGD regulation of cell growth Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Traceable author statement. Source: GOC
Molecular function	insulin-like growth factor binding Traceable author statement Ref.2. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.5
	Chain	22 – 254	233	Insulin-like growth factor-binding protein 4		PRO_0000014384
Regions
	Domain	23 – 103	81	IGFBP N-terminal
	Domain	167 – 245	79	Thyroglobulin type-1
Amino acid modifications
	Glycosylation	125	1	N-linked (GlcNAc...) Potential
	Disulfide bond	27 ↔ 53		By similarity
	Disulfide bond	30 ↔ 55		By similarity
	Disulfide bond	38 ↔ 59		Ref.6
	Disulfide bond	44 ↔ 56		Ref.6
	Disulfide bond	67 ↔ 80		Ref.6
	Disulfide bond	74 ↔ 100		Ref.6
	Disulfide bond	131 ↔ 138		Ref.6
	Disulfide bond	170 ↔ 200		Ref.6
	Disulfide bond	211 ↔ 222		Ref.6
	Disulfide bond	224 ↔ 245		Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P21744 [UniParc]. Last modified August 1, 1991. Version 2. Checksum: 97D37AC98BD0E787	FASTA	254	27,745
	10 20 30 40 50 60 MLPFGLVAAL LLAAGPRPSL GDEAIHCPPC SEEKLARCRP PVGCEELVRE PGCGCCATCA 70 80 90 100 110 120 LGLGMPCGVY TPRCGSGMRC YPPRGVEKPL RTLMHGQGVC TELSEIEAIQ ESLQTSDKDE 130 140 150 160 170 180 SEHPNNSFNP CSAHDHRCLQ KHMAKVRDRS KMKVVGTPRE EPRPVPQGSC QSELHRALER 190 200 210 220 230 240 LAASQSRTHE DLFIIPIPNC DRNGNFHPKQ CHPALDGQRG KCWCVDRKTG VKLPGGLEPK 250 GELDCHQLAD SLQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the cDNAs encoding a novel insulin-like growth factor-binding protein from rat and human." Shimasaki S., Uchiyama F., Shimonaka M., Ling N. Mol. Endocrinol. 4:1451-1458(1990) [PubMed: 1704481] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure of the rat insulin-like growth factor binding protein-4 gene." Gao L., Ling N., Shimasaki S. Biochem. Biophys. Res. Commun. 190:1053-1059(1993) [PubMed: 7679899] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley.
[3]	"IGFBP3, 4, 5 and 6 expression at the mandibular condylar cartilage during rat growth and development." Hajjar D., Ricarte-Filho J.C.M., dos Santos M.F., Kimura E.T. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Mandibular condyle.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[5]	"Identification of a novel binding protein for insulin-like growth factors in adult rat serum." Shimonaka M., Schroeder R., Shimasaki S., Ling N. Biochem. Biophys. Res. Commun. 165:189-195(1989) [PubMed: 2480123] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-61. Tissue: Serum.
[6]	"Expression, purification and characterization of the structure and disulfide linkages of insulin-like growth factor binding protein-4." Chelius D., Baldwin M.A., Lu X., Spencer E.M. J. Endocrinol. 168:283-296(2001) [PubMed: 11182766] [Abstract] Cited for: DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L08276 Genomic DNA. No translation available. AY686592 mRNA. Translation: AAT96376.1. BC098750 mRNA. Translation: AAH98750.1.
IPI	IPI00206239.
PIR	JC1464.
RefSeq	NP_001004274.1. NM_001004274.2.
UniGene	Rn.160666.
3D structure databases
ProteinModelPortal	P21744.
SMR	P21744. Positions 23-113, 168-246.
ModBase	Search...
Protein-protein interaction databases
STRING	P21744.
Proteomic databases
PRIDE	P21744.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000014153; ENSRNOP00000014153; ENSRNOG00000010635.
GeneID	360622.
KEGG	rno:360622.
NMPDR	fig|10116.3.peg.6055.
UCSC	NM_001004274. rat.
Organism-specific databases
CTD	3487.
RGD	2875. Igfbp4.
Phylogenomic databases
eggNOG	roNOG14455.
GeneTree	ENSGT00550000074457.
HOVERGEN	HBG002631.
InParanoid	P21744.
OMA	CLQKHLA.
OrthoDB	EOG4FR0SF.
PhylomeDB	P21744.
Gene expression databases
ArrayExpress	P21744.
Genevestigator	P21744.
GermOnline	ENSRNOG00000010635. Rattus norvegicus.
Family and domain databases
InterPro	IPR009030. Growth_fac_rcpt. IPR022327. IGFBP-4. IPR000867. IGFBP-like. IPR009168. IGFBP1-6. IPR022321. IGFBP_1-6_chordata. IPR017891. Insulin_GF-bd_Cys-rich_CS. IPR000716. Thyroglobulin_1. [Graphical view]
Gene3D	G3DSA:4.10.800.10. Thyroglobulin_1. 1 hit.
PANTHER	PTHR11551. IGFBP1-6. 1 hit.
Pfam	PF00219. IGFBP. 1 hit. PF00086. Thyroglobulin_1. 1 hit. [Graphical view]
PRINTS	PR01976. IGFBPFAMILY. PR01980. IGFBPFAMILY4.
SMART	SM00121. IB. 1 hit. SM00211. TY. 1 hit. [Graphical view]
SUPFAM	SSF57184. Grow_fac_recept. 1 hit. SSF57610. Thyroglobulin_1. 1 hit.
PROSITE	PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS00484. THYROGLOBULIN_1_1. 1 hit. PS51162. THYROGLOBULIN_1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	673497.

Entry information

Entry name

IBP4_RAT

Accession

Primary (citable) accession number: P21744
Secondary accession number(s): Q68J74

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	August 1, 1991
Last modified:	October 19, 2011
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program