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Protein

Insulin-like growth factor-binding protein 4

Gene

Igfbp4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

GO - Molecular functioni

  1. insulin-like growth factor binding Source: RGD
  2. insulin-like growth factor I binding Source: GO_Central
  3. insulin-like growth factor II binding Source: GO_Central

GO - Biological processi

  1. inflammatory response Source: RGD
  2. intracellular signal transduction Source: RGD
  3. positive regulation of insulin-like growth factor receptor signaling pathway Source: Ensembl
  4. positive regulation of MAPK cascade Source: Ensembl
  5. regulation of cell growth Source: Ensembl
  6. regulation of glucose metabolic process Source: Ensembl
  7. regulation of insulin-like growth factor receptor signaling pathway Source: GO_Central
  8. type B pancreatic cell proliferation Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

Growth factor binding

Enzyme and pathway databases

ReactomeiREACT_277593. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Protein family/group databases

MEROPSiI31.952.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor-binding protein 4
Short name:
IBP-4
Short name:
IGF-binding protein 4
Short name:
IGFBP-4
Gene namesi
Name:Igfbp4
Synonyms:Igfbp-4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi2875. Igfbp4.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 254233Insulin-like growth factor-binding protein 4PRO_0000014384Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi27 ↔ 53PROSITE-ProRule annotation
Disulfide bondi30 ↔ 55PROSITE-ProRule annotation
Disulfide bondi38 ↔ 59PROSITE-ProRule annotation1 Publication
Disulfide bondi44 ↔ 56PROSITE-ProRule annotation1 Publication
Disulfide bondi67 ↔ 80PROSITE-ProRule annotation1 Publication
Disulfide bondi74 ↔ 100PROSITE-ProRule annotation1 Publication
Glycosylationi125 – 1251N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi131 ↔ 138PROSITE-ProRule annotation1 Publication
Disulfide bondi170 ↔ 200PROSITE-ProRule annotation1 Publication
Disulfide bondi211 ↔ 222PROSITE-ProRule annotation1 Publication
Disulfide bondi224 ↔ 245PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP21744.
PRIDEiP21744.

Expressioni

Gene expression databases

GenevestigatoriP21744.

Interactioni

Subunit structurei

Binds IGF2 more than IGF1.

Protein-protein interaction databases

BioGridi262076. 2 interactions.
STRINGi10116.ENSRNOP00000014153.

Structurei

3D structure databases

ProteinModelPortaliP21744.
SMRiP21744. Positions 23-113, 168-246.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 10381IGFBP N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini167 – 24579Thyroglobulin type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
Contains 1 thyroglobulin type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG46496.
GeneTreeiENSGT00550000074457.
HOGENOMiHOG000253012.
HOVERGENiHBG002631.
InParanoidiP21744.
OMAiQGRTHED.
OrthoDBiEOG74N5HG.
PhylomeDBiP21744.
TreeFamiTF331211.

Family and domain databases

Gene3Di4.10.40.20. 1 hit.
4.10.800.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_N_dom.
IPR022327. IGFBP-4.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERiPTHR11551. PTHR11551. 1 hit.
PfamiPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSiPR01976. IGFBPFAMILY.
PR01980. IGFBPFAMILY4.
SMARTiSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21744-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPFGLVAAL LLAAGPRPSL GDEAIHCPPC SEEKLARCRP PVGCEELVRE
60 70 80 90 100
PGCGCCATCA LGLGMPCGVY TPRCGSGMRC YPPRGVEKPL RTLMHGQGVC
110 120 130 140 150
TELSEIEAIQ ESLQTSDKDE SEHPNNSFNP CSAHDHRCLQ KHMAKVRDRS
160 170 180 190 200
KMKVVGTPRE EPRPVPQGSC QSELHRALER LAASQSRTHE DLFIIPIPNC
210 220 230 240 250
DRNGNFHPKQ CHPALDGQRG KCWCVDRKTG VKLPGGLEPK GELDCHQLAD

SLQE
Length:254
Mass (Da):27,745
Last modified:July 31, 1991 - v2
Checksum:i97D37AC98BD0E787
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08276 Genomic DNA. No translation available.
AY686592 mRNA. Translation: AAT96376.1.
BC098750 mRNA. Translation: AAH98750.1.
PIRiJC1464.
RefSeqiNP_001004274.1. NM_001004274.2.
UniGeneiRn.160666.

Genome annotation databases

EnsembliENSRNOT00000014153; ENSRNOP00000014153; ENSRNOG00000010635.
GeneIDi360622.
KEGGirno:360622.
UCSCiRGD:2875. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08276 Genomic DNA. No translation available.
AY686592 mRNA. Translation: AAT96376.1.
BC098750 mRNA. Translation: AAH98750.1.
PIRiJC1464.
RefSeqiNP_001004274.1. NM_001004274.2.
UniGeneiRn.160666.

3D structure databases

ProteinModelPortaliP21744.
SMRiP21744. Positions 23-113, 168-246.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi262076. 2 interactions.
STRINGi10116.ENSRNOP00000014153.

Protein family/group databases

MEROPSiI31.952.

Proteomic databases

PaxDbiP21744.
PRIDEiP21744.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000014153; ENSRNOP00000014153; ENSRNOG00000010635.
GeneIDi360622.
KEGGirno:360622.
UCSCiRGD:2875. rat.

Organism-specific databases

CTDi3487.
RGDi2875. Igfbp4.

Phylogenomic databases

eggNOGiNOG46496.
GeneTreeiENSGT00550000074457.
HOGENOMiHOG000253012.
HOVERGENiHBG002631.
InParanoidiP21744.
OMAiQGRTHED.
OrthoDBiEOG74N5HG.
PhylomeDBiP21744.
TreeFamiTF331211.

Enzyme and pathway databases

ReactomeiREACT_277593. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Miscellaneous databases

NextBioi673497.
PROiP21744.

Gene expression databases

GenevestigatoriP21744.

Family and domain databases

Gene3Di4.10.40.20. 1 hit.
4.10.800.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_N_dom.
IPR022327. IGFBP-4.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERiPTHR11551. PTHR11551. 1 hit.
PfamiPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSiPR01976. IGFBPFAMILY.
PR01980. IGFBPFAMILY4.
SMARTiSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the cDNAs encoding a novel insulin-like growth factor-binding protein from rat and human."
    Shimasaki S., Uchiyama F., Shimonaka M., Ling N.
    Mol. Endocrinol. 4:1451-1458(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structure of the rat insulin-like growth factor binding protein-4 gene."
    Gao L., Ling N., Shimasaki S.
    Biochem. Biophys. Res. Commun. 190:1053-1059(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
  3. "IGFBP3, 4, 5 and 6 expression at the mandibular condylar cartilage during rat growth and development."
    Hajjar D., Ricarte-Filho J.C.M., dos Santos M.F., Kimura E.T.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Mandibular condyle.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  5. "Identification of a novel binding protein for insulin-like growth factors in adult rat serum."
    Shimonaka M., Schroeder R., Shimasaki S., Ling N.
    Biochem. Biophys. Res. Commun. 165:189-195(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-61.
    Tissue: Serum.
  6. "Expression, purification and characterization of the structure and disulfide linkages of insulin-like growth factor binding protein-4."
    Chelius D., Baldwin M.A., Lu X., Spencer E.M.
    J. Endocrinol. 168:283-296(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.

Entry informationi

Entry nameiIBP4_RAT
AccessioniPrimary (citable) accession number: P21744
Secondary accession number(s): Q68J74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 1991
Last sequence update: July 31, 1991
Last modified: March 31, 2015
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.