P21743 (IBP1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-like growth factor-binding protein 1
Short name=IBP-1
Short name=IGF-binding protein 1
Short name=IGFBP-1

Gene names

Name:	Igfbp1
Synonyms:	Igfbp-1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration By similarity.
Subunit structure	Binds equally well IGF1 and IGF2.
Subcellular location	Secreted.
Sequence similarities	Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Growth factor binding
PTM	Disulfide bond Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glucose metabolic process Traceable author statement PubMed 15350195. Source: RGD insulin receptor signaling pathway Inferred from expression pattern PubMed 11784721. Source: RGD phosphatidylinositol-mediated signaling Traceable author statement PubMed 11942857. Source: RGD positive regulation of cell growth Inferred from expression pattern Ref.2. Source: RGD tissue regeneration Inferred from expression pattern Ref.2. Source: RGD
Cellular_component	extracellular space Inferred from direct assay PubMed 16166214. Source: RGD
Molecular_function	insulin-like growth factor binding Traceable author statement PubMed 15070850. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Ref.6
	Chain	26 – 272	247	Insulin-like growth factor-binding protein 1		PRO_0000014368
Regions
	Domain	28 – 109	82	IGFBP N-terminal
	Domain	186 – 264	79	Thyroglobulin type-1
	Motif	259 – 261	3	Cell attachment site
Amino acid modifications
	Modified residue	139	1	Phosphoserine By similarity
	Modified residue	157	1	Phosphoserine By similarity
	Disulfide bond	73 ↔ 86		By similarity
	Disulfide bond	80 ↔ 106		By similarity
	Disulfide bond	189 ↔ 219		By similarity
	Disulfide bond	230 ↔ 241		By similarity
	Disulfide bond	243 ↔ 264		By similarity
Experimental info
	Sequence conflict	79	1	R → A Ref.1
	Sequence conflict	79	1	R → A Ref.4
	Sequence conflict	111 – 112	2	AA → PP Ref.1
	Sequence conflict	201	1	A → R in AAA41382. Ref.3
	Sequence conflict	265	1	H → N Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P21743 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: 12B9F3A42200753D	FASTA	272	29,684
	10 20 30 40 50 60 MPEFLTVVSW PFLILLSFQV RVVAGAPQPW HCAPCTAERL ELCPPVPASC PEISRPAGCG 70 80 90 100 110 120 CCPTCALPLG AACGVATARC AQGLSCRALP GEPRPLHALT RGQGACVLEP AAPATSSLSG 130 140 150 160 170 180 SQHEEAKAAV ASEDELAESP EMTEEQLLDS FHLMAPSRED QPILWNAIST YSSMRAREIT 190 200 210 220 230 240 DLKKWKEPCQ RELYKVLERL AAAQQKAGDE IYKFYLPNCN KNGFYHSKQC ETSLDGEAGL 250 260 270 CWCVYPWSGK KIPGSLETRG DPNCHQYFNV QN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a rat decidual insulin-like growth factor-binding protein complementary DNA." Murphy L.J., Seneviratne C., Ballejo G., Croze F., Kennedy T.G. Mol. Endocrinol. 4:329-336(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Decidua.
[2]	"The gene encoding rat insulin-like growth factor-binding protein 1 is rapidly and highly induced in regenerating liver." Mohn K.L., Melby A.E., Tewari D.S., Laz T.M., Taub R.A. Mol. Cell. Biol. 11:1393-1401(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"Insulin rapidly decreases insulin-like growth factor-binding protein-1 gene transcription in streptozotocin-diabetic rats." Ooi G.T., Tseng L.Y.H., Tran M.Q., Rechler M.M. Mol. Endocrinol. 6:2219-2228(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Dideoxy sequencing and structural analysis of the rat insulin-like growth factor binding protein-1 gene." Lacson R.G., Oehler D., Yang E., Goswami R., Unterman T.G. Biochim. Biophys. Acta 1218:95-98(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Liver.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[6]	"Production of the rat type 1 insulin-like growth factor-binding protein by well differentiated H4EIIC3 hepatoma cells: identification, purification, and N-terminal amino acid analysis." Unterman T.G., Oehler D.T., Gotway M.B., Morris P.W. Endocrinology 127:789-797(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-59.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M58634 mRNA. Translation: AAA41380.1. M89791 mRNA. Translation: AAA41382.1. L22979 Genomic DNA. Translation: AAA82581.1. BC078889 mRNA. Translation: AAH78889.1.
IPI	IPI00206238.
PIR	A36082.
RefSeq	NP_037276.1. NM_013144.1.
UniGene	Rn.34026.
3D structure databases
ProteinModelPortal	P21743.
SMR	P21743. Positions 30-108.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000011338.
Protein family/group databases
MEROPS	I31.951.
Proteomic databases
PRIDE	P21743.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000011338; ENSRNOP00000011338; ENSRNOG00000008371.
GeneID	25685.
KEGG	rno:25685.
UCSC	RGD:2872. rat.
Organism-specific databases
CTD	3484.
RGD	2872. Igfbp1.
Phylogenomic databases
eggNOG	NOG44381.
GeneTree	ENSGT00550000074457.
HOGENOM	HOG000253012.
HOVERGEN	HBG002631.
OrthoDB	EOG4W9J4F.
Gene expression databases
Genevestigator	P21743.
GermOnline	ENSRNOG00000008371. Rattus norvegicus.
Family and domain databases
Gene3D	4.10.800.10. 1 hit.
InterPro	IPR009030. Growth_fac_rcpt. IPR000867. IGFBP-like. IPR022322. IGFBP1. IPR009168. IGFBP1-6. IPR022321. IGFBP_1-6_chordata. IPR017891. Insulin_GF-bd_Cys-rich_CS. IPR000716. Thyroglobulin_1. [Graphical view]
PANTHER	PTHR11551. PTHR11551. 1 hit.
Pfam	PF00219. IGFBP. 1 hit. PF00086. Thyroglobulin_1. 1 hit. [Graphical view]
PRINTS	PR01976. IGFBPFAMILY. PR01977. IGFBPFAMILY1.
SMART	SM00121. IB. 1 hit. SM00211. TY. 1 hit. [Graphical view]
SUPFAM	SSF57184. Grow_fac_recept. 1 hit. SSF57610. Thyroglobulin_1. 1 hit.
PROSITE	PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS00484. THYROGLOBULIN_1_1. 1 hit. PS51162. THYROGLOBULIN_1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	607667.

Entry information

Entry name

IBP1_RAT

Accession

Primary (citable) accession number: P21743

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program