P21742 (THNB_HORVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-hordothionin

Cleaved into the following 2 chains:

1. Beta-hordothionin
2. Acidic protein

Gene names

Name:

THI1.2

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	136 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.
Subunit structure	Homodimer. Ref.3
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the plant thionin (TC 1.C.44) family. [View classification]

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Secreted
Domain	Signal
Molecular function	Toxin
PTM	Disulfide bond
Technical term	3D-structure
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

27

Chain

28 – 72

45

Beta-hordothionin

PRO_0000034114

Chain

73 – 136

64

Acidic protein

PRO_0000034115

Amino acid modifications

Disulfide bond

30 ↔ 66

Ref.3

Disulfide bond

31 ↔ 58

Ref.3

Disulfide bond

39 ↔ 56

Ref.3

Disulfide bond

43 ↔ 52

Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

136

Helix Strand Turn

Details...

Beta strand

29 – 33

5

Helix

34 – 43

10

Turn

44 – 46

3

Helix

49 – 56

8

Beta strand

62 – 65

4

Sequences

	Sequence		Length	Mass (Da)
	P21742 [UniParc]. Last modified July 1, 1993. Version 2. Checksum: F9E04874D44CF831	FASTA	136	14,603
	10 20 30 40 50 60 MGSKGLKGVM VCLLILGLVL EHVQVEGKSC CRSTLGRNCY NLCRVRGAQK LCANACRCKL 70 80 90 100 110 120 TSGLKCPSSF PKLALVSNSD EPDTIDYCNL GCRASMCDYM VNAAADDEEM KLYVEHCSDA 130 CVNFCNGDVG LTSLTA

References

[1]	"Full-length cDNA clone encoding barley toxin beta-hordothionin." Rasmussen S.K., Rasmussen C.E. Plant Mol. Biol. 21:579-579(1993) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Bomi. Tissue: Endosperm.
[2]	"Polyadenylation site heterogeneity in mRNA encoding the precursor of the barley toxin beta-hordothionin." Hernandez-Lucas C., Royo J., Paz-Ares J., Ponz F., Garcia-Olmedo F., Carbonero P. FEBS Lett. 200:103-106(1986) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-136.
[3]	"Crystal structure of alpha-hordothionin at 1.9 Angstrom resolution." Johnson K.A., Kim E., Teeter M.M., Suh S.W., Stec B. FEBS Lett. 579:2301-2306(2005) [PubMed: 15848162] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 28-72, DISULFIDE BOND, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z13008 mRNA. Translation: CAA78352.1.

PIR

S22977.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WUW	X-ray	1.90	A/B	28-72	[»]

ProteinModelPortal

P21742.

SMR

P21742. Positions 28-72.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Organism-specific databases

Gramene

P21742.

Gene expression databases

Genevestigator

P21742.

Family and domain databases

InterPro

IPR001010. Thionin.
[Graphical view]

Gene3D

G3DSA:3.30.1350.10. Thionin. 1 hit.

Pfam

PF00321. Thionin. 1 hit.
[Graphical view]

PRINTS

PR00287. THIONIN.

SUPFAM

SSF57429. Thionin. 1 hit.

PROSITE

PS00271. THIONIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

THNB_HORVU

Accession

Primary (citable) accession number: P21742

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	July 1, 1993
Last modified:	May 31, 2011
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program