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Protein

Ubiquitin-conjugating enzyme E2 1

Gene

UBC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. Cognate E2 conjugating enzyme for the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M).PROSITE-ProRule annotation2 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei88Glycyl thioester intermediate1

GO - Molecular functioni

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • protein polyubiquitination Source: GO_Central
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: SGD
  • vesicle organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Stress response, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29766-MONOMER.
ReactomeiR-SCE-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 1 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 1
Ubiquitin carrier protein
Ubiquitin-conjugating enzyme E2-24 kDa
Ubiquitin-protein ligase
Gene namesi
Name:UBC1
Ordered Locus Names:YDR177W
ORF Names:YD9395.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR177W.
SGDiS000002584. UBC1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nucleus Source: GO_Central
  • proteasome complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825251 – 215Ubiquitin-conjugating enzyme E2 1Add BLAST215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki93Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP21734.
PRIDEiP21734.

PTM databases

iPTMnetiP21734.

Expressioni

Developmental stagei

Early stages of growth after spore germination.

Inductioni

By heat shock and cadmium.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi32230. 39 interactors.
DIPiDIP-6594N.
IntActiP21734. 5 interactors.
MINTiMINT-591274.

Structurei

Secondary structure

1215
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 15Combined sources13
Helixi18 – 20Combined sources3
Beta strandi22 – 27Combined sources6
Beta strandi28 – 31Combined sources4
Beta strandi32 – 40Combined sources9
Beta strandi43 – 45Combined sources3
Turni46 – 49Combined sources4
Beta strandi51 – 57Combined sources7
Turni60 – 63Combined sources4
Beta strandi68 – 73Combined sources6
Beta strandi78 – 80Combined sources3
Turni82 – 84Combined sources3
Helixi90 – 92Combined sources3
Turni93 – 95Combined sources3
Helixi102 – 114Combined sources13
Beta strandi118 – 120Combined sources3
Helixi124 – 132Combined sources9
Helixi134 – 148Combined sources15
Helixi170 – 179Combined sources10
Helixi183 – 192Combined sources10
Helixi204 – 213Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FXTNMR-A2-150[»]
1FZYX-ray1.90A/B2-150[»]
1TTENMR-A1-215[»]
ProteinModelPortaliP21734.
SMRiP21734.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21734.

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00670000098059.
HOGENOMiHOG000233455.
InParanoidiP21734.
KOiK04649.
OMAiNKEIADC.
OrthoDBiEOG092C50OL.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR009060. UBA-like.
IPR015368. UBA_C_fun.
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF09288. UBA_3. 1 hit.
PF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21734-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRAKRIMKE IQAVKDDPAA HITLEFVSES DIHHLKGTFL GPPGTPYEGG
60 70 80 90 100
KFVVDIEVPM EYPFKPPKMQ FDTKVYHPNI SSVTGAICLD ILKNAWSPVI
110 120 130 140 150
TLKSALISLQ ALLQSPEPND PQDAEVAQHY LRDRESFNKT AALWTRLYAS
160 170 180 190 200
ETSNGQKGNV EESDLYGIDH DLIDEFESQG FEKDKIVEVL RRLGVKSLDP
210
NDNNTANRII EELLK
Length:215
Mass (Da):24,178
Last modified:May 1, 1991 - v1
Checksum:i899CC397A1285145
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56402 Genomic DNA. Translation: CAA39812.1.
Z46727 Genomic DNA. Translation: CAA86682.1.
AY557675 Genomic DNA. Translation: AAS56001.1.
BK006938 Genomic DNA. Translation: DAA12019.1.
PIRiS12493.
RefSeqiNP_010462.3. NM_001180484.3.

Genome annotation databases

EnsemblFungiiYDR177W; YDR177W; YDR177W.
GeneIDi851757.
KEGGisce:YDR177W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56402 Genomic DNA. Translation: CAA39812.1.
Z46727 Genomic DNA. Translation: CAA86682.1.
AY557675 Genomic DNA. Translation: AAS56001.1.
BK006938 Genomic DNA. Translation: DAA12019.1.
PIRiS12493.
RefSeqiNP_010462.3. NM_001180484.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FXTNMR-A2-150[»]
1FZYX-ray1.90A/B2-150[»]
1TTENMR-A1-215[»]
ProteinModelPortaliP21734.
SMRiP21734.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32230. 39 interactors.
DIPiDIP-6594N.
IntActiP21734. 5 interactors.
MINTiMINT-591274.

PTM databases

iPTMnetiP21734.

Proteomic databases

MaxQBiP21734.
PRIDEiP21734.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR177W; YDR177W; YDR177W.
GeneIDi851757.
KEGGisce:YDR177W.

Organism-specific databases

EuPathDBiFungiDB:YDR177W.
SGDiS000002584. UBC1.

Phylogenomic databases

GeneTreeiENSGT00670000098059.
HOGENOMiHOG000233455.
InParanoidiP21734.
KOiK04649.
OMAiNKEIADC.
OrthoDBiEOG092C50OL.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciYEAST:G3O-29766-MONOMER.
ReactomeiR-SCE-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.

Miscellaneous databases

EvolutionaryTraceiP21734.
PROiP21734.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR009060. UBA-like.
IPR015368. UBA_C_fun.
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF09288. UBA_3. 1 hit.
PF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBC1_YEAST
AccessioniPrimary (citable) accession number: P21734
Secondary accession number(s): D6VSF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 30, 2016
This is version 165 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8940 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.