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P21734

- UBC1_YEAST

UniProt

P21734 - UBC1_YEAST

Protein

Ubiquitin-conjugating enzyme E2 1

Gene

UBC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 144 (01 Oct 2014)
      Sequence version 1 (01 May 1991)
      Previous versions | rss
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    Functioni

    Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. Cognate E2 conjugating enzyme for the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M).2 PublicationsPROSITE-ProRule annotation

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881Glycyl thioester intermediate

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: SGD

    GO - Biological processi

    1. ER-associated ubiquitin-dependent protein catabolic process Source: SGD
    2. protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: SGD
    3. response to stress Source: UniProtKB-KW
    4. vesicle organization Source: SGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Stress response, Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29766-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 1 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein
    Ubiquitin-conjugating enzyme E2-24 kDa
    Ubiquitin-protein ligase
    Gene namesi
    Name:UBC1
    Ordered Locus Names:YDR177W
    ORF Names:YD9395.10
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR177w.
    SGDiS000002584. UBC1.

    Subcellular locationi

    GO - Cellular componenti

    1. proteasome complex Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 215215Ubiquitin-conjugating enzyme E2 1PRO_0000082525Add
    BLAST

    Proteomic databases

    MaxQBiP21734.
    PaxDbiP21734.
    PeptideAtlasiP21734.

    Expressioni

    Developmental stagei

    Early stages of growth after spore germination.

    Inductioni

    By heat shock and cadmium.

    Gene expression databases

    GenevestigatoriP21734.

    Interactioni

    Protein-protein interaction databases

    BioGridi32230. 29 interactions.
    DIPiDIP-6594N.
    IntActiP21734. 5 interactions.
    MINTiMINT-591274.
    STRINGi4932.YDR177W.

    Structurei

    Secondary structure

    1
    215
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 1513
    Helixi18 – 203
    Beta strandi22 – 276
    Beta strandi28 – 314
    Beta strandi32 – 409
    Beta strandi43 – 453
    Turni46 – 494
    Beta strandi51 – 577
    Turni60 – 634
    Beta strandi68 – 736
    Beta strandi78 – 803
    Turni82 – 843
    Helixi90 – 923
    Turni93 – 953
    Helixi102 – 11413
    Beta strandi118 – 1203
    Helixi124 – 1329
    Helixi134 – 14815
    Helixi170 – 17910
    Helixi183 – 19210
    Helixi204 – 21310

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FXTNMR-A2-150[»]
    1FZYX-ray1.90A/B2-150[»]
    1TTENMR-A1-215[»]
    ProteinModelPortaliP21734.
    SMRiP21734. Positions 2-215.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP21734.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00670000098059.
    HOGENOMiHOG000233455.
    KOiK04649.
    OMAiNKEIADC.
    OrthoDBiEOG7SBP18.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR009060. UBA-like.
    IPR015368. UBA_C_fun.
    IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF09288. UBA_3. 1 hit.
    PF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF46934. SSF46934. 1 hit.
    SSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21734-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRAKRIMKE IQAVKDDPAA HITLEFVSES DIHHLKGTFL GPPGTPYEGG    50
    KFVVDIEVPM EYPFKPPKMQ FDTKVYHPNI SSVTGAICLD ILKNAWSPVI 100
    TLKSALISLQ ALLQSPEPND PQDAEVAQHY LRDRESFNKT AALWTRLYAS 150
    ETSNGQKGNV EESDLYGIDH DLIDEFESQG FEKDKIVEVL RRLGVKSLDP 200
    NDNNTANRII EELLK 215
    Length:215
    Mass (Da):24,178
    Last modified:May 1, 1991 - v1
    Checksum:i899CC397A1285145
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56402 Genomic DNA. Translation: CAA39812.1.
    Z46727 Genomic DNA. Translation: CAA86682.1.
    AY557675 Genomic DNA. Translation: AAS56001.1.
    BK006938 Genomic DNA. Translation: DAA12019.1.
    PIRiS12493.
    RefSeqiNP_010462.3. NM_001180484.3.

    Genome annotation databases

    EnsemblFungiiYDR177W; YDR177W; YDR177W.
    GeneIDi851757.
    KEGGisce:YDR177W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56402 Genomic DNA. Translation: CAA39812.1 .
    Z46727 Genomic DNA. Translation: CAA86682.1 .
    AY557675 Genomic DNA. Translation: AAS56001.1 .
    BK006938 Genomic DNA. Translation: DAA12019.1 .
    PIRi S12493.
    RefSeqi NP_010462.3. NM_001180484.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FXT NMR - A 2-150 [» ]
    1FZY X-ray 1.90 A/B 2-150 [» ]
    1TTE NMR - A 1-215 [» ]
    ProteinModelPortali P21734.
    SMRi P21734. Positions 2-215.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32230. 29 interactions.
    DIPi DIP-6594N.
    IntActi P21734. 5 interactions.
    MINTi MINT-591274.
    STRINGi 4932.YDR177W.

    Proteomic databases

    MaxQBi P21734.
    PaxDbi P21734.
    PeptideAtlasi P21734.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR177W ; YDR177W ; YDR177W .
    GeneIDi 851757.
    KEGGi sce:YDR177W.

    Organism-specific databases

    CYGDi YDR177w.
    SGDi S000002584. UBC1.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00670000098059.
    HOGENOMi HOG000233455.
    KOi K04649.
    OMAi NKEIADC.
    OrthoDBi EOG7SBP18.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci YEAST:G3O-29766-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P21734.
    NextBioi 969525.
    PROi P21734.

    Gene expression databases

    Genevestigatori P21734.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR009060. UBA-like.
    IPR015368. UBA_C_fun.
    IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF09288. UBA_3. 1 hit.
    PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46934. SSF46934. 1 hit.
    SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "UBC1 encodes a novel member of an essential subfamily of yeast ubiquitin-conjugating enzymes involved in protein degradation."
      Seufert W., McGrath J.P., Jeutsch S.
      EMBO J. 9:4535-4541(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "A regulatory link between ER-associated protein degradation and the unfolded-protein response."
      Friedlander R., Jarosch E., Urban J., Volkwein C., Sommer T.
      Nat. Cell Biol. 2:379-384(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Hrd1p/Der3p is a membrane-anchored ubiquitin ligase required for ER-associated degradation."
      Bays N.W., Gardner R.G., Seelig L.P., Joazeiro C.A., Hampton R.Y.
      Nat. Cell Biol. 3:24-29(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HDR1.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Structure of a conjugating enzyme-ubiquitin thiolester intermediate reveals a novel role for the ubiquitin tail."
      Hamilton K.S., Ellison M.J., Barber K.R., Williams R.S., Huzil J.T., McKenna S., Ptak C., Glover M., Shaw G.S.
      Structure 9:897-904(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), STRUCTURE BY NMR IN COMPLEX WITH UBIQUITIN.
    10. "Solution structure of the flexible class II ubiquitin-conjugating enzyme Ubc1 provides insights for polyubiquitin chain assembly."
      Merkley N., Shaw G.S.
      J. Biol. Chem. 279:47139-47147(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 2-215.

    Entry informationi

    Entry nameiUBC1_YEAST
    AccessioniPrimary (citable) accession number: P21734
    Secondary accession number(s): D6VSF9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: May 1, 1991
    Last modified: October 1, 2014
    This is version 144 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 8940 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3