P21730 (C5AR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C5a anaphylatoxin chemotactic receptor
Short name=C5a-R
Short name=C5aR

Alternative name(s):
CD_antigen=CD88

Gene names

Name:	C5AR1
Synonyms:	C5AR, C5R1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
Subunit structure	Interacts with ARRB1 and ARRB2 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein Sulfation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of MAPK activity Traceable author statement PubMed 7649993. Source: ProtInc activation of phospholipase C activity Traceable author statement PubMed 7649993. Source: ProtInc apoptotic process Inferred from electronic annotation. Source: Compara cell proliferation in hindbrain Inferred from electronic annotation. Source: Compara cellular defense response Traceable author statement PubMed 8779720. Source: ProtInc chemotaxis Traceable author statement PubMed 10352266. Source: ProtInc defense response to Gram-positive bacterium Inferred from electronic annotation. Source: Compara elevation of cytosolic calcium ion concentration Traceable author statement PubMed 8702752. Source: ProtInc immune response Traceable author statement PubMed 9590258. Source: ProtInc inflammatory response Inferred from electronic annotation. Source: Compara mRNA transcription from RNA polymerase II promoter Inferred from direct assay PubMed 22496247. Source: UniProtKB negative regulation of neuron apoptotic process Inferred from electronic annotation. Source: Compara neutrophil chemotaxis Inferred from electronic annotation. Source: Compara organ regeneration Inferred from electronic annotation. Source: Compara positive regulation of ERK1 and ERK2 cascade Inferred from direct assay PubMed 22496247. Source: UniProtKB positive regulation of epithelial cell proliferation Inferred from direct assay PubMed 22496247. Source: UniProtKB response to lipopolysaccharide Inferred from electronic annotation. Source: Compara response to peptidoglycan Inferred from electronic annotation. Source: Compara sensory perception of chemical stimulus Traceable author statement PubMed 9590258. Source: ProtInc
Cellular_component	apical part of cell Inferred from direct assay PubMed 22496247. Source: UniProtKB basolateral plasma membrane Inferred from direct assay PubMed 22960554. Source: UniProtKB cell surface Inferred from electronic annotation. Source: Compara integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	C5a anaphylatoxin receptor activity Inferred from direct assay PubMed 22496247. Source: UniProtKB N-formyl peptide receptor activity Inferred from electronic annotation. Source: InterPro complement component C5a receptor activity Inferred from direct assay PubMed 22496247. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	C5a anaphylatoxin chemotactic receptor		PRO_0000069209
Regions
	Topological domain	1 – 37	37	Extracellular Potential
	Transmembrane	38 – 60	23	Helical; Name=1; Potential
	Topological domain	61 – 71	11	Cytoplasmic Potential
	Transmembrane	72 – 94	23	Helical; Name=2; Potential
	Topological domain	95 – 110	16	Extracellular Potential
	Transmembrane	111 – 132	22	Helical; Name=3; Potential
	Topological domain	133 – 153	21	Cytoplasmic Potential
	Transmembrane	154 – 174	21	Helical; Name=4; Potential
	Topological domain	175 – 200	26	Extracellular Potential
	Transmembrane	201 – 226	26	Helical; Name=5; Potential
	Topological domain	227 – 242	16	Cytoplasmic Potential
	Transmembrane	243 – 265	23	Helical; Name=6; Potential
	Topological domain	266 – 282	17	Extracellular Potential
	Transmembrane	283 – 303	21	Helical; Name=7; Potential
	Topological domain	304 – 350	47	Cytoplasmic Potential
Amino acid modifications
	Modified residue	11	1	Sulfotyrosine Ref.9
	Modified residue	14	1	Sulfotyrosine Ref.9
	Modified residue	314	1	Phosphoserine Probable
	Modified residue	317	1	Phosphoserine Probable
	Modified residue	327	1	Phosphoserine Probable
	Modified residue	332	1	Phosphoserine Probable
	Modified residue	334	1	Phosphoserine Probable
	Modified residue	338	1	Phosphoserine Probable
	Glycosylation	5	1	N-linked (GlcNAc...) Potential
	Disulfide bond	109 ↔ 188		By similarity
Natural variations
	Natural variant	2	1	D → N. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6 Ref.7 Corresponds to variant rs4467185 [ dbSNP | Ensembl ].		VAR_049377
	Natural variant	279	1	K → N. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6 Corresponds to variant rs11880097 [ dbSNP | Ensembl ].		VAR_049378

Sequences

	Sequence		Length	Mass (Da)
	P21730 [UniParc]. Last modified November 2, 2010. Version 2. Checksum: 9334BB39A2C96D3D	FASTA	350	39,336
	10 20 30 40 50 60 MDSFNYTTPD YGHYDDKDTL DLNTPVDKTS NTLRVPDILA LVIFAVVFLV GVLGNALVVW 70 80 90 100 110 120 VTAFEAKRTI NAIWFLNLAV ADFLSCLALP ILFTSIVQHH HWPFGGAACS ILPSLILLNM 130 140 150 160 170 180 YASILLLATI SADRFLLVFK PIWCQNFRGA GLAWIACAVA WGLALLLTIP SFLYRVVREE 190 200 210 220 230 240 YFPPKVLCGV DYSHDKRRER AVAIVRLVLG FLWPLLTLTI CYTFILLRTW SRRATRSTKT 250 260 270 280 290 300 LKVVVAVVAS FFIFWLPYQV TGIMMSFLEP SSPTFLLLKK LDSLCVSFAY INCCINPIIY 310 320 330 340 350 VVAGQGFQGR LRKSLPSLLR NVLTEESVVR ESKSFTRSTV DTMAQKTQAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression cloning of a receptor for C5a anaphylatoxin on differentiated HL-60 cells." Boulay F., Mery L., Tardif M., Brouchon L., Vignais P. Biochemistry 30:2993-2999(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ASN-2 AND ASN-279.
[2]	"The chemotactic receptor for human C5a anaphylatoxin." Gerard N.P., Gerard C. Nature 349:614-617(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS ASN-2 AND ASN-279.
[3]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ASN-2 AND ASN-279.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ASN-2 AND ASN-279.
[5]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ASN-2 AND ASN-279. Tissue: Brain.
[7]	"Human chemotaxis receptor genes cluster at 19q13.3-13.4. Characterization of the human C5a receptor gene." Gerard N.P., Bao L., Xiao-Ping H., Eddy R.L. Jr., Shows T.B., Gerard C. Biochemistry 32:1243-1250(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3, VARIANT ASN-2.
[8]	"Identification of the major phosphorylation sites in human C5a anaphylatoxin receptor in vivo." Giannini E., Brouchon L., Boulay F. J. Biol. Chem. 270:19166-19172(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-314; SER-317; SER-327; SER-332; SER-334 AND SER-338.
[9]	"Sulfated tyrosines contribute to the formation of the c5a docking site of the human c5a anaphylatoxin receptor." Farzan M., Schnitzler C.E., Vasilieva N., Leung D., Kuhn J., Gerard C., Gerard N.P., Choe H. J. Exp. Med. 193:1059-1066(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SULFATION AT TYR-11 AND TYR-14.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M62505 mRNA. Translation: AAA62831.1. X57250 mRNA. Translation: CAA40530.1. X58674 Genomic DNA. Translation: CAB37830.1. AY221091 Genomic DNA. Translation: AAO65969.1. BT007358 mRNA. Translation: AAP36022.1. AC099491 Genomic DNA. No translation available. BC008982 mRNA. Translation: AAH08982.1. S56556, S56557 Genomic DNA. Translation: AAD14919.1.
IPI	IPI00010308.
PIR	A37963.
RefSeq	NP_001727.1. NM_001736.3.
UniGene	Hs.2161. Hs.661425.
3D structure databases
ProteinModelPortal	P21730.
SMR	P21730. Positions 32-334.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000347197.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P21730.
Polymorphism databases
DMDM	311033355.
Proteomic databases
PaxDb	P21730.
PRIDE	P21730.
Protocols and materials databases
DNASU	728.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000355085; ENSP00000347197; ENSG00000197405.
GeneID	728.
KEGG	hsa:728.
UCSC	uc002pgj.1. human.
Organism-specific databases
CTD	728.
GeneCards	GC19P047813.
HGNC	HGNC:1338. C5AR1.
HPA	CAB002720. HPA014520.
MIM	113995. gene.
neXtProt	NX_P21730.
PharmGKB	PA25920.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG151055.
HOGENOM	HOG000234122.
HOVERGEN	HBG107547.
InParanoid	P21730.
KO	K04010.
OMA	YINCCIN.
OrthoDB	EOG4VQ9Q1.
PhylomeDB	P21730.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	P21730.
CleanEx	HS_C5AR1.
Genevestigator	P21730.
GermOnline	ENSG00000197405. Homo sapiens.
Family and domain databases
InterPro	IPR002234. Anphylx_rcpt. IPR001274. C5A_anaphtx_rcpt. IPR000826. Formyl_pep_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
PANTHER	PTHR24225. PTHR24225. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01104. ANPHYLATOXNR. PR00426. C5ANPHYLTXNR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P21730.
ChEMBL	CHEMBL2373.
ChiTaRS	C5AR1. human.
NextBio	2964.
SOURCE	Search...

Entry information

Entry name

C5AR_HUMAN

Accession

Primary (citable) accession number: P21730

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	November 2, 2010
Last modified:	May 1, 2013
This is version 131 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.