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P21730 (C5AR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 145. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C5a anaphylatoxin chemotactic receptor 1
Alternative name(s):
C5a anaphylatoxin chemotactic receptor
Short name=C5a-R
Short name=C5aR
CD_antigen=CD88
Gene names
Name:C5AR1
Synonyms:C5AR, C5R1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.

Subunit structure

Interacts with ARRB1 and ARRB2 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Post-translational modification

Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Biological processChemotaxis
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
Sulfation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of MAPK activity

Traceable author statement PubMed 7649993. Source: ProtInc

activation of phospholipase C activity

Traceable author statement PubMed 7649993. Source: ProtInc

apoptotic process

Inferred from electronic annotation. Source: Ensembl

cell proliferation in hindbrain

Inferred from electronic annotation. Source: Ensembl

cellular defense response

Traceable author statement PubMed 8779720. Source: ProtInc

chemotaxis

Traceable author statement PubMed 10352266. Source: ProtInc

complement component C5a signaling pathway

Inferred from direct assay PubMed 22496247. Source: GOC

defense response to Gram-positive bacterium

Inferred from electronic annotation. Source: Ensembl

immune response

Traceable author statement PubMed 9590258. Source: ProtInc

inflammatory response

Inferred from electronic annotation. Source: Ensembl

mRNA transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 22496247. Source: UniProtKB

negative regulation of neuron apoptotic process

Inferred from electronic annotation. Source: Ensembl

neutrophil chemotaxis

Inferred from electronic annotation. Source: Ensembl

organ regeneration

Inferred from electronic annotation. Source: Ensembl

positive regulation of ERK1 and ERK2 cascade

Inferred from direct assay PubMed 22496247. Source: UniProtKB

positive regulation of cytosolic calcium ion concentration

Traceable author statement PubMed 8702752. Source: ProtInc

positive regulation of epithelial cell proliferation

Inferred from direct assay PubMed 22496247. Source: UniProtKB

response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

response to peptidoglycan

Inferred from electronic annotation. Source: Ensembl

sensory perception of chemical stimulus

Traceable author statement PubMed 9590258. Source: ProtInc

signal transduction

Traceable author statement Ref.1PubMed 8702752. Source: ProtInc

   Cellular_componentapical part of cell

Inferred from direct assay PubMed 22496247. Source: UniProtKB

basolateral plasma membrane

Inferred from direct assay PubMed 22960554. Source: UniProtKB

cell surface

Inferred from electronic annotation. Source: Ensembl

integral component of plasma membrane

Traceable author statement Ref.1. Source: ProtInc

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionC5a anaphylatoxin receptor activity

Inferred from direct assay PubMed 22496247. Source: UniProtKB

N-formyl peptide receptor activity

Inferred from electronic annotation. Source: InterPro

complement component C5a receptor activity

Inferred from direct assay PubMed 22496247. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 350350C5a anaphylatoxin chemotactic receptor 1
PRO_0000069209

Regions

Topological domain1 – 3737Extracellular Potential
Transmembrane38 – 6023Helical; Name=1; Potential
Topological domain61 – 7111Cytoplasmic Potential
Transmembrane72 – 9423Helical; Name=2; Potential
Topological domain95 – 11016Extracellular Potential
Transmembrane111 – 13222Helical; Name=3; Potential
Topological domain133 – 15321Cytoplasmic Potential
Transmembrane154 – 17421Helical; Name=4; Potential
Topological domain175 – 20026Extracellular Potential
Transmembrane201 – 22626Helical; Name=5; Potential
Topological domain227 – 24216Cytoplasmic Potential
Transmembrane243 – 26523Helical; Name=6; Potential
Topological domain266 – 28217Extracellular Potential
Transmembrane283 – 30321Helical; Name=7; Potential
Topological domain304 – 35047Cytoplasmic Potential

Amino acid modifications

Modified residue111Sulfotyrosine Ref.9
Modified residue141Sulfotyrosine Ref.9
Modified residue3141Phosphoserine Probable
Modified residue3171Phosphoserine Probable
Modified residue3271Phosphoserine Probable
Modified residue3321Phosphoserine Probable
Modified residue3341Phosphoserine Probable
Modified residue3381Phosphoserine Probable
Glycosylation51N-linked (GlcNAc...) Potential
Disulfide bond109 ↔ 188 By similarity

Natural variations

Natural variant21D → N. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6 Ref.7
Corresponds to variant rs4467185 [ dbSNP | Ensembl ].
VAR_049377
Natural variant2791K → N. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6
Corresponds to variant rs11880097 [ dbSNP | Ensembl ].
VAR_049378

Sequences

Sequence LengthMass (Da)Tools
P21730 [UniParc].

Last modified November 2, 2010. Version 2.
Checksum: 9334BB39A2C96D3D

FASTA35039,336
        10         20         30         40         50         60 
MDSFNYTTPD YGHYDDKDTL DLNTPVDKTS NTLRVPDILA LVIFAVVFLV GVLGNALVVW 

        70         80         90        100        110        120 
VTAFEAKRTI NAIWFLNLAV ADFLSCLALP ILFTSIVQHH HWPFGGAACS ILPSLILLNM 

       130        140        150        160        170        180 
YASILLLATI SADRFLLVFK PIWCQNFRGA GLAWIACAVA WGLALLLTIP SFLYRVVREE 

       190        200        210        220        230        240 
YFPPKVLCGV DYSHDKRRER AVAIVRLVLG FLWPLLTLTI CYTFILLRTW SRRATRSTKT 

       250        260        270        280        290        300 
LKVVVAVVAS FFIFWLPYQV TGIMMSFLEP SSPTFLLLKK LDSLCVSFAY INCCINPIIY 

       310        320        330        340        350 
VVAGQGFQGR LRKSLPSLLR NVLTEESVVR ESKSFTRSTV DTMAQKTQAV 

« Hide

References

« Hide 'large scale' references
[1]"Expression cloning of a receptor for C5a anaphylatoxin on differentiated HL-60 cells."
Boulay F., Mery L., Tardif M., Brouchon L., Vignais P.
Biochemistry 30:2993-2999(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ASN-2 AND ASN-279.
[2]"The chemotactic receptor for human C5a anaphylatoxin."
Gerard N.P., Gerard C.
Nature 349:614-617(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS ASN-2 AND ASN-279.
[3]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Kopatz S.A., Aronstam R.S., Sharma S.V.
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ASN-2 AND ASN-279.
[4]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ASN-2 AND ASN-279.
[5]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ASN-2 AND ASN-279.
Tissue: Brain.
[7]"Human chemotaxis receptor genes cluster at 19q13.3-13.4. Characterization of the human C5a receptor gene."
Gerard N.P., Bao L., Xiao-Ping H., Eddy R.L. Jr., Shows T.B., Gerard C.
Biochemistry 32:1243-1250(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3, VARIANT ASN-2.
[8]"Identification of the major phosphorylation sites in human C5a anaphylatoxin receptor in vivo."
Giannini E., Brouchon L., Boulay F.
J. Biol. Chem. 270:19166-19172(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-314; SER-317; SER-327; SER-332; SER-334 AND SER-338.
[9]"Sulfated tyrosines contribute to the formation of the c5a docking site of the human c5a anaphylatoxin receptor."
Farzan M., Schnitzler C.E., Vasilieva N., Leung D., Kuhn J., Gerard C., Gerard N.P., Choe H.
J. Exp. Med. 193:1059-1066(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SULFATION AT TYR-11 AND TYR-14.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M62505 mRNA. Translation: AAA62831.1.
X57250 mRNA. Translation: CAA40530.1.
X58674 Genomic DNA. Translation: CAB37830.1.
AY221091 Genomic DNA. Translation: AAO65969.1.
BT007358 mRNA. Translation: AAP36022.1.
AC099491 Genomic DNA. No translation available.
BC008982 mRNA. Translation: AAH08982.1.
S56556, S56557 Genomic DNA. Translation: AAD14919.1.
CCDSCCDS33063.1.
PIRA37963.
RefSeqNP_001727.1. NM_001736.3.
UniGeneHs.2161.

3D structure databases

ProteinModelPortalP21730.
SMRP21730. Positions 32-308.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107189. 6 interactions.
STRING9606.ENSP00000347197.

Chemistry

BindingDBP21730.
ChEMBLCHEMBL2373.
GuidetoPHARMACOLOGY32.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP21730.

Polymorphism databases

DMDM311033355.

Proteomic databases

PaxDbP21730.
PRIDEP21730.

Protocols and materials databases

DNASU728.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000355085; ENSP00000347197; ENSG00000197405.
GeneID728.
KEGGhsa:728.
UCSCuc002pgj.1. human.

Organism-specific databases

CTD728.
GeneCardsGC19P047813.
HGNCHGNC:1338. C5AR1.
HPACAB002720.
HPA014520.
MIM113995. gene.
neXtProtNX_P21730.
PharmGKBPA25920.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG151055.
HOGENOMHOG000234122.
HOVERGENHBG107547.
InParanoidP21730.
KOK04010.
OMAYINCCIN.
PhylomeDBP21730.
TreeFamTF330976.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
SignaLinkP21730.

Gene expression databases

BgeeP21730.
CleanExHS_C5AR1.
GenevestigatorP21730.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR001274. Anaphtx_C5AR1/C5AR2.
IPR002234. Anphylx_rcpt.
IPR000826. Formyl_rcpt-rel.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24225. PTHR24225. 1 hit.
PTHR24225:SF28. PTHR24225:SF28. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR01104. ANPHYLATOXNR.
PR00426. C5ANPHYLTXNR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSC5AR1. human.
GeneWikiC5a_receptor.
GenomeRNAi728.
NextBio2964.
PROP21730.
SOURCESearch...

Entry information

Entry nameC5AR1_HUMAN
AccessionPrimary (citable) accession number: P21730
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 2, 2010
Last modified: July 9, 2014
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries