C5a anaphylatoxin chemotactic receptor

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P21730 (C5AR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 117. History...

Clusters with 100%, 90%, 50% identity |

Documents (7) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
C5a anaphylatoxin chemotactic receptor
Short name=C5a-R
Short name=C5aR

Alternative name(s):
CD_antigen=CD88

Gene names

Name:	C5AR1
Synonyms:	C5AR, C5R1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
Subunit structure	Interacts with ARRB1 and ARRB2 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein Sulfation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	activation of MAPK activity Traceable author statement. Source: ProtInc activation of phospholipase C activity Traceable author statement. Source: ProtInc cellular defense response Traceable author statement. Source: ProtInc elevation of cytosolic calcium ion concentration Traceable author statement. Source: ProtInc immune response Traceable author statement. Source: ProtInc sensory perception of chemical stimulus Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Traceable author statement. Source: ProtInc
Molecular function	C5a anaphylatoxin receptor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 350

350

C5a anaphylatoxin chemotactic receptor

PRO_0000069209

Regions

Topological domain

1 – 37

Extracellular Potential

Transmembrane

38 – 60

Helical; Name=1; Potential

Topological domain

61 – 71

Cytoplasmic Potential

Transmembrane

72 – 94

Helical; Name=2; Potential

Topological domain

95 – 110

Extracellular Potential

Transmembrane

111 – 132

Helical; Name=3; Potential

Topological domain

133 – 153

Cytoplasmic Potential

Transmembrane

154 – 174

Helical; Name=4; Potential

Topological domain

175 – 200

Extracellular Potential

Transmembrane

201 – 226

Helical; Name=5; Potential

Topological domain

227 – 242

Cytoplasmic Potential

Transmembrane

243 – 265

Helical; Name=6; Potential

Topological domain

266 – 282

Extracellular Potential

Transmembrane

283 – 303

Helical; Name=7; Potential

Topological domain

304 – 350

Cytoplasmic Potential

Amino acid modifications

Modified residue

Sulfotyrosine Ref.9

Modified residue

Sulfotyrosine Ref.9

Modified residue

314

Phosphoserine Probable

Modified residue

317

Phosphoserine Probable

Modified residue

327

Phosphoserine Probable

Modified residue

332

Phosphoserine Probable

Modified residue

334

Phosphoserine Probable

Modified residue

338

Phosphoserine Probable

Glycosylation

N-linked (GlcNAc...) Potential

Disulfide bond

109 ↔ 188

By similarity

Natural variations

Natural variant

D → N. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6 Ref.7
Corresponds to variant rs4467185 [ dbSNP | Ensembl ].

VAR_049377

Natural variant

279

K → N. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6
Corresponds to variant rs11880097 [ dbSNP | Ensembl ].

VAR_049378

Sequences

Sequence

Length

Mass (Da)

Tools

P21730 [UniParc].

Last modified November 2, 2010. Version 2.
Checksum: 9334BB39A2C96D3D
Show »

FASTA

350

39,336

        10         20         30         40         50         60 
MDSFNYTTPD YGHYDDKDTL DLNTPVDKTS NTLRVPDILA LVIFAVVFLV GVLGNALVVW 

        70         80         90        100        110        120 
VTAFEAKRTI NAIWFLNLAV ADFLSCLALP ILFTSIVQHH HWPFGGAACS ILPSLILLNM 

       130        140        150        160        170        180 
YASILLLATI SADRFLLVFK PIWCQNFRGA GLAWIACAVA WGLALLLTIP SFLYRVVREE 

       190        200        210        220        230        240 
YFPPKVLCGV DYSHDKRRER AVAIVRLVLG FLWPLLTLTI CYTFILLRTW SRRATRSTKT 

       250        260        270        280        290        300 
LKVVVAVVAS FFIFWLPYQV TGIMMSFLEP SSPTFLLLKK LDSLCVSFAY INCCINPIIY 

       310        320        330        340        350 
VVAGQGFQGR LRKSLPSLLR NVLTEESVVR ESKSFTRSTV DTMAQKTQAV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression cloning of a receptor for C5a anaphylatoxin on differentiated HL-60 cells." Boulay F., Mery L., Tardif M., Brouchon L., Vignais P. Biochemistry 30:2993-2999(1991) [PubMed: 2007135] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ASN-2 AND ASN-279.
[2]	"The chemotactic receptor for human C5a anaphylatoxin." Gerard N.P., Gerard C. Nature 349:614-617(1991) [PubMed: 1847994] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS ASN-2 AND ASN-279.
[3]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ASN-2 AND ASN-279.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ASN-2 AND ASN-279.
[5]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ASN-2 AND ASN-279. Tissue: Brain.
[7]	"Human chemotaxis receptor genes cluster at 19q13.3-13.4. Characterization of the human C5a receptor gene." Gerard N.P., Bao L., Xiao-Ping H., Eddy R.L. Jr., Shows T.B., Gerard C. Biochemistry 32:1243-1250(1993) [PubMed: 8383526] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3, VARIANT ASN-2.
[8]	"Identification of the major phosphorylation sites in human C5a anaphylatoxin receptor in vivo." Giannini E., Brouchon L., Boulay F. J. Biol. Chem. 270:19166-19172(1995) [PubMed: 7642584] [Abstract] Cited for: PHOSPHORYLATION AT SER-314; SER-317; SER-327; SER-332; SER-334 AND SER-338.
[9]	"Sulfated tyrosines contribute to the formation of the c5a docking site of the human c5a anaphylatoxin receptor." Farzan M., Schnitzler C.E., Vasilieva N., Leung D., Kuhn J., Gerard C., Gerard N.P., Choe H. J. Exp. Med. 193:1059-1066(2001) [PubMed: 11342590] [Abstract] Cited for: SULFATION AT TYR-11 AND TYR-14.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M62505 mRNA. Translation: AAA62831.1. X57250 mRNA. Translation: CAA40530.1. X58674 Genomic DNA. Translation: CAB37830.1. AY221091 Genomic DNA. Translation: AAO65969.1. BT007358 mRNA. Translation: AAP36022.1. AC099491 Genomic DNA. No translation available. BC008982 mRNA. Translation: AAH08982.1. S56556, S56557 Genomic DNA. Translation: AAD14919.1.
IPI	IPI00010308.
PIR	A37963.
RefSeq	NP_001727.1. NM_001736.3.
UniGene	Hs.2161.
3D structure databases
ProteinModelPortal	P21730.
SMR	P21730. Positions 39-318.
ModBase	Search...
Protein-protein interaction databases
STRING	P21730.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P21730.
Polymorphism databases
DMDM	311033355.
Proteomic databases
PRIDE	P21730.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000355085; ENSP00000347197; ENSG00000197405.
GeneID	728.
KEGG	hsa:728.
UCSC	uc002pgj.1. human.
Organism-specific databases
CTD	728.
GeneCards	GC19P047813.
H-InvDB	HIX0015270.
HGNC	HGNC:1338. C5AR1.
HPA	CAB002720. HPA014520.
MIM	113995. gene.
neXtProt	NX_P21730.
PharmGKB	PA25920.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15798.
GeneTree	ENSGT00560000076935.
HOGENOM	HBG715940.
HOVERGEN	HBG107547.
InParanoid	P21730.
OMA	NPIWCQN.
OrthoDB	EOG4VQ9Q1.
PhylomeDB	P21730.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	P21730.
Bgee	P21730.
CleanEx	HS_C5AR1.
Genevestigator	P21730.
GermOnline	ENSG00000197405. Homo sapiens.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR002234. Anphylx_rcpt. IPR001274. C5A_anaphtx_rcpt. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
KO	K04010.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01104. ANPHYLATOXNR. PR00426. C5ANPHYLTXNR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	2964.
SOURCE	Search...

Entry information

Entry name

C5AR_HUMAN

Accession

Primary (citable) accession number: P21730

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	November 2, 2010
Last modified:	January 25, 2012
This is version 117 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.