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Protein

Gastrin-releasing peptide receptor

Gene

Grpr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for gastrin releasing peptide (GRP). This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

GO - Molecular functioni

  1. bombesin receptor activity Source: MGI

GO - Biological processi

  1. regulation of cell proliferation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_207651. G alpha (q) signalling events.
REACT_235286. Peptide ligand-binding receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Gastrin-releasing peptide receptor
Short name:
GRP-R
Alternative name(s):
GRP-preferring bombesin receptor
Gene namesi
Name:Grpr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:95836. Grpr.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3939ExtracellularSequence AnalysisAdd
BLAST
Transmembranei40 – 6324Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini64 – 7714CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei78 – 9720Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini98 – 11518ExtracellularSequence AnalysisAdd
BLAST
Transmembranei116 – 13722Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini138 – 15316CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei154 – 17522Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini176 – 20934ExtracellularSequence AnalysisAdd
BLAST
Transmembranei210 – 23526Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini236 – 26530CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei266 – 28621Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini287 – 29913ExtracellularSequence AnalysisAdd
BLAST
Transmembranei300 – 32627Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini327 – 38458CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 384384Gastrin-releasing peptide receptorPRO_0000069663Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi5 – 51N-linked (GlcNAc...)Sequence Analysis
Glycosylationi20 – 201N-linked (GlcNAc...)Sequence Analysis
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi114 ↔ 197PROSITE-ProRule annotation
Lipidationi340 – 3401S-palmitoyl cysteineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PRIDEiP21729.

PTM databases

PhosphoSiteiP21729.

Expressioni

Tissue specificityi

Brain (hypothalamus), pancreatic acinar cells, and fibroblasts.

Gene expression databases

BgeeiP21729.
CleanExiMM_GRPR.
ExpressionAtlasiP21729. baseline and differential.
GenevestigatoriP21729.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Oprm1P42866-94EBI-6049651,EBI-6049667

Protein-protein interaction databases

IntActiP21729. 1 interaction.
STRINGi10090.ENSMUSP00000033730.

Structurei

3D structure databases

ProteinModelPortaliP21729.
SMRiP21729. Positions 11-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG328649.
HOGENOMiHOG000230781.
HOVERGENiHBG004898.
InParanoidiP21729.
KOiK04169.
OMAiQLLCCQP.
OrthoDBiEOG73V6KG.
PhylomeDBiP21729.
TreeFamiTF331292.

Family and domain databases

InterProiIPR001556. Bombsn_rcpt.
IPR001966. Gastrin_pep_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00358. BOMBESINR.
PR00640. GASTRINRELPR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21729-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPNNCSHLN LDVDPFLSCN DTFNQSLSPP KMDNWFHPGF IYVIPAVYGL
60 70 80 90 100
IIVIGLIGNI TLIKIFCTVK SMRNVPNLFI SSLALGDLLL LVTCAPVDAS
110 120 130 140 150
KYLADRWLFG RIGCKLIPFI QLTSVGVSVF TLTALSADRY KAIVRPMDIQ
160 170 180 190 200
ASHALMKICL KAALIWIVSM LLAIPEAVFS DLHPFHVKDT NQTFISCAPY
210 220 230 240 250
PHSNELHPKI HSMASFLVFY VIPLAIISVY YYFIARNLIQ SAYNLPVEGN
260 270 280 290 300
IHVKKQIESR KRLAKTVLVF VGLFAFCWLP NHVIYLYRSY HYSEVDTSML
310 320 330 340 350
HFVTSICARL LAFTNSCVNP FALYLLSKSF RKQFNTQLLC CQPGLMNRSH
360 370 380
STGRSTTCMT SFKSTNPSAT FSLINRNICH EGYV
Length:384
Mass (Da):43,215
Last modified:August 1, 1991 - v2
Checksum:iBF6D60387AA09A2C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti309 – 3091R → H (PubMed:1707129).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57922 mRNA. Translation: AAA75650.1.
M61000 mRNA. Translation: AAA37744.1.
CCDSiCCDS30512.1.
PIRiA39003.
RefSeqiNP_032203.1. NM_008177.2.
UniGeneiMm.4687.

Genome annotation databases

EnsembliENSMUST00000033730; ENSMUSP00000033730; ENSMUSG00000031364.
GeneIDi14829.
KEGGimmu:14829.
UCSCiuc009uuu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57922 mRNA. Translation: AAA75650.1.
M61000 mRNA. Translation: AAA37744.1.
CCDSiCCDS30512.1.
PIRiA39003.
RefSeqiNP_032203.1. NM_008177.2.
UniGeneiMm.4687.

3D structure databases

ProteinModelPortaliP21729.
SMRiP21729. Positions 11-343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP21729. 1 interaction.
STRINGi10090.ENSMUSP00000033730.

Chemistry

BindingDBiP21729.
ChEMBLiCHEMBL3596.
GuidetoPHARMACOLOGYi39.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP21729.

Proteomic databases

PRIDEiP21729.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033730; ENSMUSP00000033730; ENSMUSG00000031364.
GeneIDi14829.
KEGGimmu:14829.
UCSCiuc009uuu.1. mouse.

Organism-specific databases

CTDi2925.
MGIiMGI:95836. Grpr.

Phylogenomic databases

eggNOGiNOG328649.
HOGENOMiHOG000230781.
HOVERGENiHBG004898.
InParanoidiP21729.
KOiK04169.
OMAiQLLCCQP.
OrthoDBiEOG73V6KG.
PhylomeDBiP21729.
TreeFamiTF331292.

Enzyme and pathway databases

ReactomeiREACT_207651. G alpha (q) signalling events.
REACT_235286. Peptide ligand-binding receptors.

Miscellaneous databases

NextBioi287043.
PROiP21729.
SOURCEiSearch...

Gene expression databases

BgeeiP21729.
CleanExiMM_GRPR.
ExpressionAtlasiP21729. baseline and differential.
GenevestigatoriP21729.

Family and domain databases

InterProiIPR001556. Bombsn_rcpt.
IPR001966. Gastrin_pep_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00358. BOMBESINR.
PR00640. GASTRINRELPR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of the bombesin/gastrin-releasing peptide receptor from Swiss 3T3 cells."
    Battey J.F., Way J.M., Corjay M.H., Shapira H., Kusano K., Harkins R., Wu J.M., Slattery T., Mann E., Feldman R.I.
    Proc. Natl. Acad. Sci. U.S.A. 88:395-399(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Swiss.
    Tissue: Fibroblast.
  2. "Cloning and functional characterization of a complementary DNA encoding the murine fibroblast bombesin/gastrin-releasing peptide receptor."
    Spindel E.R., Giladi E., Brehm P., Goodman R.H., Segerson T.P.
    Mol. Endocrinol. 4:1956-1963(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss.
    Tissue: Fibroblast.
  3. "Molecular cloning and characterization of receptors for the mammalian bombesin-like peptides."
    Giladi E., Nagalla S.R., Spindel E.R.
    J. Mol. Neurosci. 4:41-54(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiGRPR_MOUSE
AccessioniPrimary (citable) accession number: P21729
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: August 1, 1991
Last modified: March 4, 2015
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.