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P21729 (GRPR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gastrin-releasing peptide receptor

Short name=GRP-R
Alternative name(s):
GRP-preferring bombesin receptor
Gene names
Name:Grpr
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length384 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for gastrin releasing peptide (GRP). This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Brain (hypothalamus), pancreatic acinar cells, and fibroblasts.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Oprm1P42866-94EBI-6049651,EBI-6049667

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 384384Gastrin-releasing peptide receptor
PRO_0000069663

Regions

Topological domain1 – 3939Extracellular Potential
Transmembrane40 – 6324Helical; Name=1; Potential
Topological domain64 – 7714Cytoplasmic Potential
Transmembrane78 – 9720Helical; Name=2; Potential
Topological domain98 – 11518Extracellular Potential
Transmembrane116 – 13722Helical; Name=3; Potential
Topological domain138 – 15316Cytoplasmic Potential
Transmembrane154 – 17522Helical; Name=4; Potential
Topological domain176 – 20934Extracellular Potential
Transmembrane210 – 23526Helical; Name=5; Potential
Topological domain236 – 26530Cytoplasmic Potential
Transmembrane266 – 28621Helical; Name=6; Potential
Topological domain287 – 29913Extracellular Potential
Transmembrane300 – 32627Helical; Name=7; Potential
Topological domain327 – 38458Cytoplasmic Potential

Amino acid modifications

Lipidation3401S-palmitoyl cysteine By similarity
Glycosylation51N-linked (GlcNAc...) Potential
Glycosylation201N-linked (GlcNAc...) Potential
Glycosylation241N-linked (GlcNAc...) Potential
Disulfide bond114 ↔ 197 By similarity

Experimental info

Sequence conflict3091R → H Ref.2

Sequences

Sequence LengthMass (Da)Tools
P21729 [UniParc].

Last modified August 1, 1991. Version 2.
Checksum: BF6D60387AA09A2C

FASTA38443,215
        10         20         30         40         50         60 
MAPNNCSHLN LDVDPFLSCN DTFNQSLSPP KMDNWFHPGF IYVIPAVYGL IIVIGLIGNI 

        70         80         90        100        110        120 
TLIKIFCTVK SMRNVPNLFI SSLALGDLLL LVTCAPVDAS KYLADRWLFG RIGCKLIPFI 

       130        140        150        160        170        180 
QLTSVGVSVF TLTALSADRY KAIVRPMDIQ ASHALMKICL KAALIWIVSM LLAIPEAVFS 

       190        200        210        220        230        240 
DLHPFHVKDT NQTFISCAPY PHSNELHPKI HSMASFLVFY VIPLAIISVY YYFIARNLIQ 

       250        260        270        280        290        300 
SAYNLPVEGN IHVKKQIESR KRLAKTVLVF VGLFAFCWLP NHVIYLYRSY HYSEVDTSML 

       310        320        330        340        350        360 
HFVTSICARL LAFTNSCVNP FALYLLSKSF RKQFNTQLLC CQPGLMNRSH STGRSTTCMT 

       370        380 
SFKSTNPSAT FSLINRNICH EGYV 

« Hide

References

[1]"Molecular cloning of the bombesin/gastrin-releasing peptide receptor from Swiss 3T3 cells."
Battey J.F., Way J.M., Corjay M.H., Shapira H., Kusano K., Harkins R., Wu J.M., Slattery T., Mann E., Feldman R.I.
Proc. Natl. Acad. Sci. U.S.A. 88:395-399(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: Swiss.
Tissue: Fibroblast.
[2]"Cloning and functional characterization of a complementary DNA encoding the murine fibroblast bombesin/gastrin-releasing peptide receptor."
Spindel E.R., Giladi E., Brehm P., Goodman R.H., Segerson T.P.
Mol. Endocrinol. 4:1956-1963(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Swiss.
Tissue: Fibroblast.
[3]"Molecular cloning and characterization of receptors for the mammalian bombesin-like peptides."
Giladi E., Nagalla S.R., Spindel E.R.
J. Mol. Neurosci. 4:41-54(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M57922 mRNA. Translation: AAA75650.1.
M61000 mRNA. Translation: AAA37744.1.
CCDSCCDS30512.1.
PIRA39003.
RefSeqNP_032203.1. NM_008177.2.
UniGeneMm.4687.

3D structure databases

ProteinModelPortalP21729.
SMRP21729. Positions 11-343.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP21729. 1 interaction.
STRING10090.ENSMUSP00000033730.

Chemistry

BindingDBP21729.
ChEMBLCHEMBL3596.
GuidetoPHARMACOLOGY39.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP21729.

Proteomic databases

PRIDEP21729.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033730; ENSMUSP00000033730; ENSMUSG00000031364.
GeneID14829.
KEGGmmu:14829.
UCSCuc009uuu.1. mouse.

Organism-specific databases

CTD2925.
MGIMGI:95836. Grpr.

Phylogenomic databases

eggNOGNOG328649.
HOGENOMHOG000230781.
HOVERGENHBG004898.
InParanoidP21729.
KOK04169.
OMACNISNHS.
OrthoDBEOG73V6KG.
PhylomeDBP21729.
TreeFamTF331292.

Gene expression databases

BgeeP21729.
CleanExMM_GRPR.
GenevestigatorP21729.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR001556. Bombsn_rcpt.
IPR001966. Gastrin_pep_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00358. BOMBESINR.
PR00640. GASTRINRELPR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio287043.
PROP21729.
SOURCESearch...

Entry information

Entry nameGRPR_MOUSE
AccessionPrimary (citable) accession number: P21729
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: August 1, 1991
Last modified: July 9, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries