P21729 (GRPR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gastrin-releasing peptide receptor
Short name=GRP-R

Alternative name(s):
GRP-preferring bombesin receptor

Gene names

Name:

Grpr

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for gastrin releasing peptide (GRP). This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Brain (hypothalamus), pancreatic acinar cells, and fibroblasts.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	regulation of cell proliferation Inferred from mutant phenotype PubMed 11960700. Source: MGI
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	bombesin receptor activity Traceable author statement PubMed 11960700. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Oprm1	P42866-9	4	EBI-6049651,EBI-6049667

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 384	384	Gastrin-releasing peptide receptor		PRO_0000069663
Regions
	Topological domain	1 – 39	39	Extracellular Potential
	Transmembrane	40 – 63	24	Helical; Name=1; Potential
	Topological domain	64 – 77	14	Cytoplasmic Potential
	Transmembrane	78 – 97	20	Helical; Name=2; Potential
	Topological domain	98 – 115	18	Extracellular Potential
	Transmembrane	116 – 137	22	Helical; Name=3; Potential
	Topological domain	138 – 153	16	Cytoplasmic Potential
	Transmembrane	154 – 175	22	Helical; Name=4; Potential
	Topological domain	176 – 209	34	Extracellular Potential
	Transmembrane	210 – 235	26	Helical; Name=5; Potential
	Topological domain	236 – 265	30	Cytoplasmic Potential
	Transmembrane	266 – 286	21	Helical; Name=6; Potential
	Topological domain	287 – 299	13	Extracellular Potential
	Transmembrane	300 – 326	27	Helical; Name=7; Potential
	Topological domain	327 – 384	58	Cytoplasmic Potential
Amino acid modifications
	Lipidation	340	1	S-palmitoyl cysteine By similarity
	Glycosylation	5	1	N-linked (GlcNAc...) Potential
	Glycosylation	20	1	N-linked (GlcNAc...) Potential
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Disulfide bond	114 ↔ 197		By similarity
Experimental info
	Sequence conflict	309	1	R → H Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P21729 [UniParc]. Last modified August 1, 1991. Version 2. Checksum: BF6D60387AA09A2C	FASTA	384	43,215
	10 20 30 40 50 60 MAPNNCSHLN LDVDPFLSCN DTFNQSLSPP KMDNWFHPGF IYVIPAVYGL IIVIGLIGNI 70 80 90 100 110 120 TLIKIFCTVK SMRNVPNLFI SSLALGDLLL LVTCAPVDAS KYLADRWLFG RIGCKLIPFI 130 140 150 160 170 180 QLTSVGVSVF TLTALSADRY KAIVRPMDIQ ASHALMKICL KAALIWIVSM LLAIPEAVFS 190 200 210 220 230 240 DLHPFHVKDT NQTFISCAPY PHSNELHPKI HSMASFLVFY VIPLAIISVY YYFIARNLIQ 250 260 270 280 290 300 SAYNLPVEGN IHVKKQIESR KRLAKTVLVF VGLFAFCWLP NHVIYLYRSY HYSEVDTSML 310 320 330 340 350 360 HFVTSICARL LAFTNSCVNP FALYLLSKSF RKQFNTQLLC CQPGLMNRSH STGRSTTCMT 370 380 SFKSTNPSAT FSLINRNICH EGYV

References

[1]	"Molecular cloning of the bombesin/gastrin-releasing peptide receptor from Swiss 3T3 cells." Battey J.F., Way J.M., Corjay M.H., Shapira H., Kusano K., Harkins R., Wu J.M., Slattery T., Mann E., Feldman R.I. Proc. Natl. Acad. Sci. U.S.A. 88:395-399(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Swiss. Tissue: Fibroblast.
[2]	"Cloning and functional characterization of a complementary DNA encoding the murine fibroblast bombesin/gastrin-releasing peptide receptor." Spindel E.R., Giladi E., Brehm P., Goodman R.H., Segerson T.P. Mol. Endocrinol. 4:1956-1963(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Swiss. Tissue: Fibroblast.
[3]	"Molecular cloning and characterization of receptors for the mammalian bombesin-like peptides." Giladi E., Nagalla S.R., Spindel E.R. J. Mol. Neurosci. 4:41-54(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M57922 mRNA. Translation: AAA75650.1. M61000 mRNA. Translation: AAA37744.1.
IPI	IPI00127458.
PIR	A39003.
RefSeq	NP_032203.1. NM_008177.2.
UniGene	Mm.4687.
3D structure databases
ProteinModelPortal	P21729.
SMR	P21729. Positions 11-341.
ModBase	Search...
Protein-protein interaction databases
IntAct	P21729. 1 interaction.
STRING	10090.ENSMUSP00000033730.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P21729.
Proteomic databases
PRIDE	P21729.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000033730; ENSMUSP00000033730; ENSMUSG00000031364.
GeneID	14829.
KEGG	mmu:14829.
UCSC	uc009uuu.1. mouse.
Organism-specific databases
CTD	2925.
MGI	MGI:95836. Grpr.
Phylogenomic databases
eggNOG	NOG328649.
HOGENOM	HOG000230781.
HOVERGEN	HBG004898.
InParanoid	P21729.
KO	K04169.
OrthoDB	EOG476K0C.
Gene expression databases
ArrayExpress	P21729.
Bgee	P21729.
CleanEx	MM_GRPR.
Genevestigator	P21729.
GermOnline	ENSMUSG00000031364. Mus musculus.
Family and domain databases
InterPro	IPR001556. Bombsn_rcpt. IPR001966. Gastrin_pep_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00358. BOMBESINR. PR00640. GASTRINRELPR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P21729.
ChEMBL	CHEMBL3596.
NextBio	287043.
SOURCE	Search...

Entry information

Entry name

GRPR_MOUSE

Accession

Primary (citable) accession number: P21729

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	August 1, 1991
Last modified:	May 29, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program