Reviewed,
UniProtKB/Swiss-Prot P21708 (MK03_RAT)
Last modified
November 3, 2009.
Version 115.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Mitogen-activated protein kinase 3 EC=2.7.11.24 Alternative name(s): Extracellular signal-regulated kinase 1 Short name=ERK-1 Insulin-stimulated MAP2 kinase MAP kinase 1 Short name=MAPK 1 p44-ERK1 ERT2 p44-MAPK Microtubule-associated protein 2 kinase MNK1 | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 380 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1. Phosphorylates heat shock factor protein 4 (HSF4) By similarity. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Cofactor | Magnesium By similarity. |
| Enzyme regulation | Activated and tyrosine phosphorylated in response to insulin and NGF. |
| Subunit structure | Interacts with NISCH, MORG1 and HSF4 By similarity. |
| Subcellular location | Isoform 2: Nucleus. |
| Tissue specificity | Highest levels within the nervous system, expressed in different tissues, mostly in intestine, placenta and lung. |
| Developmental stage | Increased expression during development. |
| Domain | The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. |
| Post-translational modification | Dually phosphorylated on Thr-203 and Tyr-205, which activates the enzyme. Ref.3 Ref.9 Ref.10 Ref.11 |
| Sequence similarities | Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: P21708-1) Also known as: A; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: P21708-2) Also known as: B; The sequence of this isoform differs from the canonical sequence as follows: 340-340: E → EVSRPPAAGRGISVPSVRPVPYCLCPQ |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.3 | ||||||
| Chain | 2 – 380 | 379 | Mitogen-activated protein kinase 3 | PRO_0000186253 | |||||
Regions | |||||||||
| Domain | 43 – 331 | 289 | Protein kinase | ||||||
| Nucleotide binding | 49 – 57 | 9 | ATP By similarity | ||||||
| Motif | 203 – 205 | 3 | TXY | ||||||
Sites | |||||||||
| Active site | 167 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 72 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylalanine Ref.3 | ||||||
| Modified residue | 203 | 1 | Phosphothreonine Ref.3 Ref.11 | ||||||
| Modified residue | 205 | 1 | Phosphotyrosine Ref.3 Ref.11 | ||||||
Natural variations | |||||||||
| Alternative sequence | 340 | 1 | E → EVSRPPAAGRGISVPSVRPV PYCLCPQ in isoform 2. | VSP_004830 | |||||
Experimental info | |||||||||
| Sequence conflict | 95 | 1 | R → G Ref.1 | ||||||
| Sequence conflict | 95 | 1 | R → G Ref.4 | ||||||
| Sequence conflict | 95 | 1 | R → G Ref.7 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence of a rat cDNA encoding the ERK1-MAP kinase." Marquardt B., Stabel S. Gene 120:297-299(1992) [PubMed: 1327976] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: Sprague-Dawley. Tissue: Brain. |
| [2] | "ERK1b, a 46-kDa ERK isoform that is differentially regulated by MEK." Yung Y., Yao Z., Hanoch T., Seger R. J. Biol. Chem. 275:15799-15808(2000) [PubMed: 10748187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). |
| [3] | Bienvenut W.V., von Kriegsheim A.F., Kolch W. Submitted (AUG-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-65; 96-132; 157-209; 213-221; 280-357 AND 361-380 (ISOFORM 2), CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, PHOSPHORYLATION AT THR-203 AND TYR-205, MASS SPECTROMETRY. Tissue: Pheochromocytoma. |
| [4] | Maisonpierre P.C., le Beau M.M., Espinosa R. III, Ip N.Y., Belluscio L., la Monte S.M., Squinto S., Furth M.E., Yancopoulos G.D. Submitted (JUL-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-380 (ISOFORM 1). |
| [5] | Lubec G., Afjehi-Sadat L., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 73-84 AND 183-190, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain and Spinal cord. |
| [6] | "An insulin-stimulated protein kinase similar to yeast kinases involved in cell cycle control." Boulton T.G., Yancopoulos G.D., Gregory J.S., Slaughter C., Moomaw C., Hsu J., Cobb M.H. Science 249:64-67(1990) [PubMed: 2164259] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-380 (ISOFORM 1), PARTIAL PROTEIN SEQUENCE. |
| [7] | "Molecular analysis of microtubule-associated protein-2 kinase cDNA from mouse and rat brain." de Miguel C., Kligman D., Patel J., Detera-Wadleigh S.D. DNA Cell Biol. 10:505-514(1991) [PubMed: 1716439] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-380 (ISOFORM 1). Tissue: Brain cortex. |
| [8] | "Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase." Boulton T.G., Gregory J.S., Cobb M.H. Biochemistry 30:278-286(1991) [PubMed: 1846291] [Abstract] Cited for: PROTEIN SEQUENCE OF 43-64 AND 167-185, CHARACTERIZATION. |
| [9] | "Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: implications for their mechanism of activation." Seger R., Ahn N.G., Boulton T.G., Yancopoulos G.D., Panayotatos N., Radziejewska E., Ericsson L., Bratlien R.L., Cobb M.H., Krebs E.G. Proc. Natl. Acad. Sci. U.S.A. 88:6142-6146(1991) [PubMed: 1712480] [Abstract] Cited for: AUTOPHOSPHORYLATION. |
| [10] | "PHAS-I as a link between mitogen-activated protein kinase and translation initiation." Lin T.-A., Kong X., Haystead T.A.J., Pause A., Belsham G.J., Sonenberg N., Lawrence J.C. Jr. Science 266:653-656(1994) [PubMed: 7939721] [Abstract] Cited for: PHOSPHORYLATION OF EIF4EBP1. |
| [11] | "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites." Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A. Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed: 16641100] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-203 AND TYR-205, MASS SPECTROMETRY. Tissue: Kidney. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| S46779 mRNA. Translation: AAA11604.1. X65198 mRNA. Translation: CAA46318.1. AF155236 mRNA. Translation: AAF71666.1. M61177 mRNA. Translation: AAA63486.1. M38194 mRNA. Translation: AAA41123.1. U12008 mRNA. Translation: AAA20009.1. | |
| IPI | IPI00206172. IPI00231081. |
| PIR | JC1451. |
| RefSeq | NP_059043.1. |
| UniGene | Rn.2592 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1PME based on UniProtKB P28482. |
| SMR | P21708. Positions 30-374. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:487N. |
| STRING | P21708. |
PTM databases | |
| PhosphoSite | P21708. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000026627; ENSRNOP00000026627; ENSRNOG00000019601; Rattus norvegicus. [Genome view] |
| GeneID | 50689. |
| KEGG | rno:50689. |
| NMPDR | fig|10116.3.peg.2990. |
| UCSC | NM_017347. rat. |
Organism-specific databases | |
| CTD | 50689. |
| RGD | 3046. Mapk3. |
Phylogenomic databases | |
| HOVERGEN | P21708. |
| OMA | EALETEP. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.24. 248. |
Gene expression databases | |
| ArrayExpress | P21708. |
| Genevestigator | P21708. |
| GermOnline | ENSRNOG00000019601. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR008349. Erk_1_2_MAPK. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view] |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| PRINTS | PR01770. ERK1ERK2MAPK. |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 610550. |
Entry information
| Entry name | MK03_RAT | ||||||||
| Accession | Primary (citable) accession number: P21708 Secondary accession number(s): Q4PIY8, Q62686, Q9JJ13 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

Clusters with


