SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P21707

- SYT1_RAT

UniProt

P21707 - SYT1_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Synaptotagmin-1
Gene
Syt1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2.

Cofactori

Binds 3 calcium ions per subunit. The ions are bound to the C2 domains.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi171 – 1711Calcium 2; via carbonyl oxygen
Metal bindingi172 – 1721Calcium 1
Metal bindingi172 – 1721Calcium 2
Metal bindingi178 – 1781Calcium 1
Metal bindingi230 – 2301Calcium 1
Metal bindingi230 – 2301Calcium 2
Metal bindingi231 – 2311Calcium 1; via carbonyl oxygen
Metal bindingi232 – 2321Calcium 1
Metal bindingi232 – 2321Calcium 2
Metal bindingi232 – 2321Calcium 3
Metal bindingi235 – 2351Calcium 3
Metal bindingi236 – 2361Calcium 3; via carbonyl oxygen
Metal bindingi238 – 2381Calcium 2
Metal bindingi238 – 2381Calcium 3

GO - Molecular functioni

  1. SNARE binding Source: RGD
  2. calcium ion binding Source: RGD
  3. calcium-dependent phospholipid binding Source: BHF-UCL
  4. calcium-dependent protein binding Source: RGD
  5. calmodulin binding Source: RGD
  6. clathrin binding Source: BHF-UCL
  7. identical protein binding Source: IntAct
  8. phosphatidylinositol-4,5-bisphosphate binding Source: RGD
  9. phosphatidylserine binding Source: RGD
  10. phospholipid binding Source: RGD
  11. protein C-terminus binding Source: RGD
  12. protein binding Source: UniProtKB
  13. syntaxin binding Source: BHF-UCL
  14. syntaxin-1 binding Source: RGD
  15. transporter activity Source: InterPro

GO - Biological processi

  1. calcium ion-dependent exocytosis Source: RGD
  2. detection of calcium ion Source: RGD
  3. positive regulation of calcium ion-dependent exocytosis Source: RGD
  4. positive regulation of vesicle fusion Source: RGD
  5. regulation of calcium ion-dependent exocytosis Source: RGD
  6. response to calcium ion Source: RGD
  7. synaptic vesicle exocytosis Source: RGD
  8. vesicle docking Source: RGD
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_227883. Glutamate Neurotransmitter Release Cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-1
Alternative name(s):
Synaptotagmin I
Short name:
SytI
p65
Gene namesi
Name:Syt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 7

Organism-specific databases

RGDi3803. Syt1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5757Vesicular Reviewed prediction
Add
BLAST
Transmembranei58 – 7922Helical; Reviewed prediction
Add
BLAST
Topological domaini80 – 421342Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. chromaffin granule membrane Source: UniProtKB-SubCell
  3. cytoplasm Source: MGI
  4. dense core granule Source: MGI
  5. excitatory synapse Source: BHF-UCL
  6. integral component of membrane Source: UniProtKB-KW
  7. intracellular organelle Source: RGD
  8. neuron projection Source: Ensembl
  9. plasma membrane Source: BHF-UCL
  10. presynaptic membrane Source: Ensembl
  11. secretory granule Source: RGD
  12. synaptic vesicle Source: BHF-UCL
  13. synaptic vesicle membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 421421Synaptotagmin-1
PRO_0000183940Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi24 – 241N-linked (GlcNAc...)
Lipidationi74 – 741S-palmitoyl cysteine1 Publication
Lipidationi75 – 751S-palmitoyl cysteine1 Publication
Lipidationi77 – 771S-palmitoyl cysteine1 Publication
Lipidationi79 – 791S-palmitoyl cysteine1 Publication
Lipidationi82 – 821S-palmitoyl cysteine1 Publication
Modified residuei128 – 1281Phosphothreonine By similarity
Modified residuei229 – 2291Phosphotyrosine By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP21707.
PRIDEiP21707.

PTM databases

PhosphoSiteiP21707.

Expressioni

Tissue specificityi

Predominantly expressed in rostral, phylogenetically younger brain regions, and in some endocrine tissues.

Gene expression databases

ArrayExpressiP21707.
GenevestigatoriP21707.

Interactioni

Subunit structurei

Homotetramer Inferred. Interacts with SCAMP5 and STON2. Forms a complex with SV2B, syntaxin 1 and SNAP25 By similarity. Interacts with RIMS1. Interacts with SV2A, SV2B and SV2C.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-458098,EBI-458098
Ap2a2P184845EBI-458098,EBI-539360
Cacna1bQ022942EBI-458098,EBI-540038
Scn1bQ009542EBI-458098,EBI-2619363
Scn2aP047752EBI-458098,EBI-2619448
STON2Q8WXE92EBI-458098,EBI-539742From a different organism.
Sv2aQ025632EBI-458098,EBI-466194
Syt4P502323EBI-458098,EBI-540118

Protein-protein interaction databases

BioGridi247745. 1 interaction.
DIPiDIP-29064N.
IntActiP21707. 16 interactions.
MINTiMINT-4542559.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi144 – 1529
Turni153 – 1564
Beta strandi157 – 16711
Beta strandi179 – 1868
Beta strandi205 – 2128
Helixi216 – 2194
Beta strandi223 – 2308
Beta strandi233 – 2353
Beta strandi238 – 2469
Helixi247 – 2493
Beta strandi256 – 2616
Beta strandi275 – 2839
Turni284 – 2874
Beta strandi288 – 29811
Beta strandi310 – 3189
Beta strandi321 – 3277
Beta strandi338 – 3469
Helixi349 – 3546
Beta strandi356 – 3638
Beta strandi366 – 3683
Beta strandi371 – 3799
Helixi384 – 39512
Beta strandi396 – 3994
Beta strandi401 – 4066
Helixi410 – 4178

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BYNNMR-A140-267[»]
1K5WNMR-A270-421[»]
1RSYX-ray1.90A132-266[»]
1TJMX-ray1.18A271-421[»]
1TJXX-ray1.04A271-421[»]
1UOVX-ray1.65A271-421[»]
1UOWX-ray1.04A271-421[»]
2YOAX-ray1.50A/B271-421[»]
ProteinModelPortaliP21707.
SMRiP21707. Positions 140-418.

Miscellaneous databases

EvolutionaryTraceiP21707.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini143 – 244102C2 1
Add
BLAST
Domaini274 – 377104C2 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni135 – 381247Phospholipid binding Inferred
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi80 – 11940Lys-rich
Add
BLAST

Domaini

The first C2 domain mediates Ca2+-dependent phospholipid binding.1 Publication
The second C2 domain mediates interaction with SV2A and probably with STN2.1 Publication

Sequence similaritiesi

Belongs to the synaptotagmin family.
Contains 2 C2 domains.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5038.
GeneTreeiENSGT00620000087641.
HOGENOMiHOG000232127.
HOVERGENiHBG005010.
InParanoidiP21707.
KOiK15290.
OMAiHDIIGEY.
OrthoDBiEOG78PV8W.
PhylomeDBiP21707.
TreeFamiTF315600.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR015428. Synaptotagmin1.
[Graphical view]
PANTHERiPTHR10024:SF183. PTHR10024:SF183. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21707-1 [UniParc]FASTAAdd to Basket

« Hide

MVSASHPEAL AAPVTTVATL VPHNATEPAS PGEGKEDAFS KLKQKFMNEL    50
HKIPLPPWAL IAIAIVAVLL VVTCCFCVCK KCLFKKKNKK KGKEKGGKNA 100
INMKDVKDLG KTMKDQALKD DDAETGLTDG EEKEEPKEEE KLGKLQYSLD 150
YDFQNNQLLV GIIQAAELPA LDMGGTSDPY VKVFLLPDKK KKFETKVHRK 200
TLNPVFNEQF TFKVPYSELG GKTLVMAVYD FDRFSKHDII GEFKVPMNTV 250
DFGHVTEEWR DLQSAEKEEQ EKLGDICFSL RYVPTAGKLT VVILEAKNLK 300
KMDVGGLSDP YVKIHLMQNG KRLKKKKTTI KKNTLNPYYN ESFSFEVPFE 350
QIQKVQVVVT VLDYDKIGKN DAIGKVFVGY NSTGAELRHW SDMLANPRRP 400
IAQWHTLQVE EEVDAMLAVK K 421
Length:421
Mass (Da):47,399
Last modified:May 2, 2006 - v3
Checksum:i06CE28F04C97A722
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti188 – 1881D → E in CAA36981. 1 Publication
Sequence conflicti374 – 3741G → D in CAA36981. 1 Publication
Sequence conflicti393 – 3931M → I in CAA36981. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52772 mRNA. Translation: CAA36981.1.
AJ617615 mRNA. Translation: CAE85101.1.
DQ181550 mRNA. Translation: ABA00482.1.
PIRiS09595.
RefSeqiNP_001028852.2. NM_001033680.2.
UniGeneiRn.216272.

Genome annotation databases

EnsembliENSRNOT00000048880; ENSRNOP00000049624; ENSRNOG00000006426.
GeneIDi25716.
KEGGirno:25716.
UCSCiRGD:3803. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52772 mRNA. Translation: CAA36981.1 .
AJ617615 mRNA. Translation: CAE85101.1 .
DQ181550 mRNA. Translation: ABA00482.1 .
PIRi S09595.
RefSeqi NP_001028852.2. NM_001033680.2.
UniGenei Rn.216272.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BYN NMR - A 140-267 [» ]
1K5W NMR - A 270-421 [» ]
1RSY X-ray 1.90 A 132-266 [» ]
1TJM X-ray 1.18 A 271-421 [» ]
1TJX X-ray 1.04 A 271-421 [» ]
1UOV X-ray 1.65 A 271-421 [» ]
1UOW X-ray 1.04 A 271-421 [» ]
2YOA X-ray 1.50 A/B 271-421 [» ]
ProteinModelPortali P21707.
SMRi P21707. Positions 140-418.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247745. 1 interaction.
DIPi DIP-29064N.
IntActi P21707. 16 interactions.
MINTi MINT-4542559.

PTM databases

PhosphoSitei P21707.

Proteomic databases

PaxDbi P21707.
PRIDEi P21707.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000048880 ; ENSRNOP00000049624 ; ENSRNOG00000006426 .
GeneIDi 25716.
KEGGi rno:25716.
UCSCi RGD:3803. rat.

Organism-specific databases

CTDi 6857.
RGDi 3803. Syt1.

Phylogenomic databases

eggNOGi COG5038.
GeneTreei ENSGT00620000087641.
HOGENOMi HOG000232127.
HOVERGENi HBG005010.
InParanoidi P21707.
KOi K15290.
OMAi HDIIGEY.
OrthoDBi EOG78PV8W.
PhylomeDBi P21707.
TreeFami TF315600.

Enzyme and pathway databases

Reactomei REACT_227883. Glutamate Neurotransmitter Release Cycle.

Miscellaneous databases

EvolutionaryTracei P21707.
NextBioi 607797.
PROi P21707.

Gene expression databases

ArrayExpressi P21707.
Genevestigatori P21707.

Family and domain databases

Gene3Di 2.60.40.150. 2 hits.
InterProi IPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR015428. Synaptotagmin1.
[Graphical view ]
PANTHERi PTHR10024:SF183. PTHR10024:SF183. 1 hit.
Pfami PF00168. C2. 2 hits.
[Graphical view ]
PRINTSi PR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTi SM00239. C2. 2 hits.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 2 hits.
PROSITEi PS50004. C2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Phospholipid binding by a synaptic vesicle protein homologous to the regulatory region of protein kinase C."
    Perin M.S., Fried V.A., Mignery G.A., Jahn R., Suedhof T.C.
    Nature 345:260-263(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Synaptotagmin gene content of the sequenced genomes."
    Craxton M.A.
    BMC Genomics 5:43-43(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Functional analysis of synaptotagmin."
    Sunitha S.S., Thekkuveettil A.
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Wistar.
  4. Lubec G., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 214-233; 289-297; 355-366 AND 376-388, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.
  5. "Isoform-specific, calcium-regulated interaction of the synaptic vesicle proteins SV2 and synaptotagmin."
    Schivell A.E., Batchelor R.H., Bajjalieh S.M.
    J. Biol. Chem. 271:27770-27775(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SV2A, DOMAIN.
  6. "Direct interaction of the Rab3 effector RIM with Ca2+ channels, SNAP-25, and synaptotagmin."
    Coppola T., Magnin-Luethi S., Perret-Menoud V., Gattesco S., Schiavo G., Regazzi R.
    J. Biol. Chem. 276:32756-32762(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RIMS1.
    Tissue: Brain.
  7. "Palmitoylation sites and processing of synaptotagmin I, the putative calcium sensor for neurosecretion."
    Heindel U., Schmidt M.F., Veit M.
    FEBS Lett. 544:57-62(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-74; CYS-75; CYS-77; CYS-79 AND CYS-82.
  8. Cited for: INTERACTION WITH SV2A; SV2B AND SV2C.
  9. "Structure of the first C2 domain of synaptotagmin I: a novel Ca2+/phospholipid-binding fold."
    Sutton R.B., Davletov B.A., Berghuis A.M., Suedhof T.C., Sprang S.R.
    Cell 80:929-938(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 132-266.
  10. "Solution structures of the Ca2+-free and Ca2+-bound C2A domain of synaptotagmin I: does Ca2+ induce a conformational change?"
    Shao X., Fernandez I., Suedhof T.C., Rizo J.
    Biochemistry 37:16106-16115(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 140-267.
  11. "Three-dimensional structure of the synaptotagmin 1 C2B-domain: synaptotagmin 1 as a phospholipid binding machine."
    Fernandez I., Arac D., Ubach J., Gerber S.H., Shin O., Gao Y., Anderson R.G., Suedhof T.C., Rizo J.
    Neuron 32:1057-1069(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 270-421.

Entry informationi

Entry nameiSYT1_RAT
AccessioniPrimary (citable) accession number: P21707
Secondary accession number(s): Q3S2E6, Q707P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 2, 2006
Last modified: September 3, 2014
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi