P21704 (DNAS1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyribonuclease-1
EC=3.1.21.1

Alternative name(s):
Deoxyribonuclease I
Short name=DNase I

Gene names

Name:	Dnase1
Synonyms:	Dnl1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization.
Catalytic activity	Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products.
Cofactor	Divalent cations. Prefers calcium or magnesium.
Subcellular location	Secreted. Nucleus envelope. Note: Secretory protein, stored in zymogen granules and found in the nuclear envelope.
Sequence similarities	Belongs to the DNase I family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Nucleus Secreted
Domain	Signal
Ligand	Actin-binding Calcium
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA catabolic process Inferred from electronic annotation. Source: InterPro apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear envelope Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	deoxyribonuclease I activity Inferred from direct assay PubMed 12005024. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22
	Chain	23 – 284	262	Deoxyribonuclease-1		PRO_0000007281
Sites
	Active site	100	1	By similarity
	Active site	156	1	By similarity
Amino acid modifications
	Glycosylation	40	1	N-linked (GlcNAc...) Potential
	Glycosylation	128	1	N-linked (GlcNAc...) Potential
	Disulfide bond	123 ↔ 126		By similarity
	Disulfide bond	195 ↔ 231		Essential for enzymatic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21704 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 45075AFF133781FA	FASTA	284	32,064
	10 20 30 40 50 60 MRYTGLMGIL LTLVNLLQLA ATLRIAAFNI RTFGDTKMSN ATLSSYIVKI LSRYDIAVVQ 70 80 90 100 110 120 EVRDTHLVAV GKLLDELNRD IPDNYRYIIS EPLGRKSYKE QYLFVYRPSQ VSVLDSYHYD 130 140 150 160 170 180 DGCEPCGNDT FSREPAIVKF FSPYTEVREF AIVPLHSAPT EAVSEIDALY DVYLDVRQKW 190 200 210 220 230 240 GLEDIMFMGD FNAGCSYVTS SQWSSIRLRT SPIFQWLIPD SADTTATSTH CAYDRIVVAG 250 260 270 280 ALLQAAVVPS SAVPFDFQAE YRLTNQMAEA ISDHYPVEVT LRKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a full length cDNA clone encoding the deoxyribonuclease I from the rat parotid gland." Polzar B., Mannherz H.G. Nucleic Acids Res. 18:7151-7151(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Parotid gland.
[2]	"DNase I primary transcript is alternatively spliced in both normal and apoptotic cells: no evidence of up-regulation in apoptosis." Liu Q.Y., Ribecco M., Hou Y., Walker P.R., Sikorska M. DNA Cell Biol. 16:911-918(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley.
[3]	"Identification and expression of deoxyribonuclease (DNase) I alternative transcripts in the rat." Basnakian A.G., Singh A.B., Shah S.V. Gene 289:87-96(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[5]	"Characterization of the endogenous deoxyribonuclease involved in nuclear DNA degradation during apoptosis (programmed cell death)." Peitsch M.C., Polzar B., Stephan H., Crompton T., McDonald H.R., Mannherz H.G., Tschopp J. EMBO J. 12:371-377(1993) [PubMed] [Europe PMC] [Abstract] Cited for: INVOLVEMENT IN APOPTOSIS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56060 mRNA. No translation available. U76635 Genomic DNA. Translation: AAB71495.1. AF397150 mRNA. Translation: AAK97471.1. AF397151 mRNA. Translation: AAK97472.1. BC089764 mRNA. Translation: AAH89764.1.
IPI	IPI00230876.
PIR	S13676.
RefSeq	NP_037229.1. NM_013097.2.
UniGene	Rn.6077.
3D structure databases
ProteinModelPortal	P21704.
SMR	P21704. Positions 23-281.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000009283.
Proteomic databases
PaxDb	P21704.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000009283; ENSRNOP00000009283; ENSRNOG00000006873.
GeneID	25633.
KEGG	rno:25633.
UCSC	RGD:2510. rat.
Organism-specific databases
CTD	1773.
RGD	2510. Dnase1.
Phylogenomic databases
eggNOG	NOG46375.
GeneTree	ENSGT00390000013146.
HOGENOM	HOG000059570.
HOVERGEN	HBG051368.
InParanoid	P21704.
KO	K11994.
OrthoDB	EOG4ZS93V.
Enzyme and pathway databases
BRENDA	3.1.21.1. 5301.
Gene expression databases
Genevestigator	P21704.
GermOnline	ENSRNOG00000006873. Rattus norvegicus.
Family and domain databases
InterPro	IPR018057. Deoxyribonuclease-1_AS. IPR016202. DNase_I-like. IPR008185. DNase_I_euk. IPR005135. Endo/exonuclease/phosphatase. [Graphical view]
PANTHER	PTHR11371. PTHR11371. 1 hit.
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
PIRSF	PIRSF000988. DNase_I_euk. 1 hit.
PRINTS	PR00130. DNASEI.
SMART	SM00476. DNaseIc. 1 hit. [Graphical view]
SUPFAM	SSF56219. Exo_endo_phos. 1 hit.
PROSITE	PS00919. DNASE_I_1. 1 hit. PS00918. DNASE_I_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	607443.

Entry information

Entry name

DNAS1_RAT

Accession

Primary (citable) accession number: P21704
Secondary accession number(s): Q5FVU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program