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Protein

Deoxyribonuclease-1

Gene

Dnase1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization.

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products.

Cofactori

Ca2+, Mg2+Note: Divalent metal cations. Prefers Ca2+ or Mg2+.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei100By similarity1
Active sitei156By similarity1

GO - Molecular functioni

  • deoxyribonuclease activity Source: CACAO
  • deoxyribonuclease I activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

Actin-binding, Calcium

Enzyme and pathway databases

BRENDAi3.1.21.1. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyribonuclease-1 (EC:3.1.21.1)
Alternative name(s):
Deoxyribonuclease I
Short name:
DNase I
Gene namesi
Name:Dnase1
Synonyms:Dnl1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi2510. Dnase1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Add BLAST22
ChainiPRO_000000728123 – 284Deoxyribonuclease-1Add BLAST262

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi40N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi123 ↔ 126By similarity
Glycosylationi128N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi195 ↔ 231Essential for enzymatic activityBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP21704.
PRIDEiP21704.

Expressioni

Gene expression databases

BgeeiENSRNOG00000006873.
GenevisibleiP21704. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009283.

Structurei

3D structure databases

ProteinModelPortaliP21704.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNase I family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHHA. Eukaryota.
ENOG410ZR9A. LUCA.
GeneTreeiENSGT00390000013146.
HOGENOMiHOG000059570.
HOVERGENiHBG051368.
InParanoidiP21704.
KOiK11994.
OMAiTSTHCAY.
OrthoDBiEOG091G0I0B.
PhylomeDBiP21704.
TreeFamiTF329541.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR018057. Deoxyribonuclease-1_AS.
IPR016202. DNase_I.
IPR033125. DNASE_I_2.
IPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PANTHERiPTHR11371. PTHR11371. 1 hit.
PIRSFiPIRSF000988. DNase_I_euk. 1 hit.
PRINTSiPR00130. DNASEI.
SMARTiSM00476. DNaseIc. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
PROSITEiPS00919. DNASE_I_1. 1 hit.
PS00918. DNASE_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21704-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYTGLMGIL LTLVNLLQLA ATLRIAAFNI RTFGDTKMSN ATLSSYIVKI
60 70 80 90 100
LSRYDIAVVQ EVRDTHLVAV GKLLDELNRD IPDNYRYIIS EPLGRKSYKE
110 120 130 140 150
QYLFVYRPSQ VSVLDSYHYD DGCEPCGNDT FSREPAIVKF FSPYTEVREF
160 170 180 190 200
AIVPLHSAPT EAVSEIDALY DVYLDVRQKW GLEDIMFMGD FNAGCSYVTS
210 220 230 240 250
SQWSSIRLRT SPIFQWLIPD SADTTATSTH CAYDRIVVAG ALLQAAVVPS
260 270 280
SAVPFDFQAE YRLTNQMAEA ISDHYPVEVT LRKT
Length:284
Mass (Da):32,064
Last modified:May 1, 1991 - v1
Checksum:i45075AFF133781FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56060 mRNA. No translation available.
U76635 Genomic DNA. Translation: AAB71495.1.
AF397150 mRNA. Translation: AAK97471.1.
AF397151 mRNA. Translation: AAK97472.1.
BC089764 mRNA. Translation: AAH89764.1.
PIRiS13676.
RefSeqiNP_037229.1. NM_013097.2.
XP_006245891.1. XM_006245829.3.
UniGeneiRn.6077.

Genome annotation databases

EnsembliENSRNOT00000009283; ENSRNOP00000009283; ENSRNOG00000006873.
GeneIDi25633.
KEGGirno:25633.
UCSCiRGD:2510. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56060 mRNA. No translation available.
U76635 Genomic DNA. Translation: AAB71495.1.
AF397150 mRNA. Translation: AAK97471.1.
AF397151 mRNA. Translation: AAK97472.1.
BC089764 mRNA. Translation: AAH89764.1.
PIRiS13676.
RefSeqiNP_037229.1. NM_013097.2.
XP_006245891.1. XM_006245829.3.
UniGeneiRn.6077.

3D structure databases

ProteinModelPortaliP21704.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009283.

Proteomic databases

PaxDbiP21704.
PRIDEiP21704.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009283; ENSRNOP00000009283; ENSRNOG00000006873.
GeneIDi25633.
KEGGirno:25633.
UCSCiRGD:2510. rat.

Organism-specific databases

CTDi1773.
RGDi2510. Dnase1.

Phylogenomic databases

eggNOGiENOG410IHHA. Eukaryota.
ENOG410ZR9A. LUCA.
GeneTreeiENSGT00390000013146.
HOGENOMiHOG000059570.
HOVERGENiHBG051368.
InParanoidiP21704.
KOiK11994.
OMAiTSTHCAY.
OrthoDBiEOG091G0I0B.
PhylomeDBiP21704.
TreeFamiTF329541.

Enzyme and pathway databases

BRENDAi3.1.21.1. 5301.

Miscellaneous databases

PROiP21704.

Gene expression databases

BgeeiENSRNOG00000006873.
GenevisibleiP21704. RN.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR018057. Deoxyribonuclease-1_AS.
IPR016202. DNase_I.
IPR033125. DNASE_I_2.
IPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PANTHERiPTHR11371. PTHR11371. 1 hit.
PIRSFiPIRSF000988. DNase_I_euk. 1 hit.
PRINTSiPR00130. DNASEI.
SMARTiSM00476. DNaseIc. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
PROSITEiPS00919. DNASE_I_1. 1 hit.
PS00918. DNASE_I_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNAS1_RAT
AccessioniPrimary (citable) accession number: P21704
Secondary accession number(s): Q5FVU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.