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P21696

- GPD1_SCHPO

UniProt

P21696 - GPD1_SCHPO

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Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)] 1

Gene

gpd1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei121 – 1211NADBy similarity
Binding sitei144 – 1441NAD; via amide nitrogenBy similarity
Binding sitei144 – 1441SubstrateBy similarity
Binding sitei177 – 1771NAD; via amide nitrogenBy similarity
Active sitei232 – 2321Proton acceptorBy similarity
Binding sitei296 – 2961NADBy similarity
Binding sitei325 – 3251NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 346NADBy similarity

GO - Molecular functioni

  1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: PomBase
  2. NAD binding Source: InterPro

GO - Biological processi

  1. cellular response to osmotic stress Source: PomBase
  2. cellular water homeostasis Source: PomBase
  3. glycerol-3-phosphate catabolic process Source: InterPro
  4. glycerol biosynthetic process Source: PomBase
  5. NADH oxidation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_201900. Synthesis of PA.
REACT_224904. Triglyceride Biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)] 1 (EC:1.1.1.8)
Alternative name(s):
GPDH-C
Short name:
GPD-C
Gene namesi
Name:gpd1
ORF Names:SPBC215.05
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC215.05.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
  3. glycerol-3-phosphate dehydrogenase complex Source: InterPro
  4. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 385385Glycerol-3-phosphate dehydrogenase [NAD(+)] 1PRO_0000138095Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei376 – 3761Phosphoserine1 Publication
Modified residuei382 – 3821Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP21696.
PaxDbiP21696.

Interactioni

Protein-protein interaction databases

BioGridi276983. 45 interactions.
MINTiMINT-4687624.
STRINGi4896.SPBC215.05-1.

Structurei

3D structure databases

ProteinModelPortaliP21696.
SMRiP21696. Positions 24-371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni296 – 2972Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0240.
HOGENOMiHOG000246855.
InParanoidiP21696.
KOiK00006.
OMAiCKLLSTS.
OrthoDBiEOG7V76H7.
PhylomeDBiP21696.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11728. PTHR11728. 1 hit.
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21696-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGYGQQGVS AANIDSIRPK KRLSIGVVGS GNWGTAIAKI CGENARAHGH
60 70 80 90 100
HFRSKVRMWV FEEEIEYKGE KRKLTEVFNE AHENVKYLPG IECPPNVIAV
110 120 130 140 150
PDVREVARRA DILVFVVPHQ FIERVCDQMV GLIRPGAVGI SCIKGVAVSK
160 170 180 190 200
EGVRLYSEVI SEKLGIYCGV LSGANVANEV AREQFCETTI GFNPPNEVDI
210 220 230 240 250
PREQIAAVFD RPYFSVVSVD DVAGVALGGA LKNVVAMAVG FADGLEWGGN
260 270 280 290 300
TKAAIMRRGL LEMQKFATTF FDSDPRTMVE QSCGIADLVT SCLGGRNNRC
310 320 330 340 350
AEAFVKTGKS LETLEKELLG GQLLQGAATS KDVHEFLLTK DMVKDFPLFT
360 370 380
AVYNISYEDM DPKDLIIVLQ PLKEDSENEG GTETE
Length:385
Mass (Da):41,995
Last modified:January 11, 2001 - v2
Checksum:i8061C294196516E1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 563SKV → GKG in CAA39630. (PubMed:2263480)Curated
Sequence conflicti126 – 1272CD → WH in CAA39630. (PubMed:2263480)Curated
Sequence conflicti153 – 1531Missing in CAA39630. (PubMed:2263480)Curated
Sequence conflicti154 – 1541R → S in CAA39630. (PubMed:2263480)Curated
Sequence conflicti209 – 2091F → S in CAA39630. (PubMed:2263480)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56162 Genomic DNA. Translation: CAA39630.1.
CU329671 Genomic DNA. Translation: CAA22119.1.
D50796 Genomic DNA. Translation: BAA09424.1.
PIRiT39895.
RefSeqiNP_596682.1. NM_001022605.2.

Genome annotation databases

EnsemblFungiiSPBC215.05.1; SPBC215.05.1:pep; SPBC215.05.
GeneIDi2540455.
KEGGispo:SPBC215.05.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56162 Genomic DNA. Translation: CAA39630.1 .
CU329671 Genomic DNA. Translation: CAA22119.1 .
D50796 Genomic DNA. Translation: BAA09424.1 .
PIRi T39895.
RefSeqi NP_596682.1. NM_001022605.2.

3D structure databases

ProteinModelPortali P21696.
SMRi P21696. Positions 24-371.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 276983. 45 interactions.
MINTi MINT-4687624.
STRINGi 4896.SPBC215.05-1.

Proteomic databases

MaxQBi P21696.
PaxDbi P21696.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC215.05.1 ; SPBC215.05.1:pep ; SPBC215.05 .
GeneIDi 2540455.
KEGGi spo:SPBC215.05.

Organism-specific databases

PomBasei SPBC215.05.

Phylogenomic databases

eggNOGi COG0240.
HOGENOMi HOG000246855.
InParanoidi P21696.
KOi K00006.
OMAi CKLLSTS.
OrthoDBi EOG7V76H7.
PhylomeDBi P21696.

Enzyme and pathway databases

Reactomei REACT_201900. Synthesis of PA.
REACT_224904. Triglyceride Biosynthesis.

Miscellaneous databases

NextBioi 20801582.

Family and domain databases

Gene3Di 1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProi IPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11728. PTHR11728. 1 hit.
Pfami PF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSi PR00077. GPDHDRGNASE.
SUPFAMi SSF48179. SSF48179. 1 hit.
TIGRFAMsi TIGR03376. glycerol3P_DH. 1 hit.
PROSITEi PS00957. NAD_G3PDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Glycerol-3-phosphate dehydrogenase homologue from Schizosaccharomyces pombe."
    Pidoux A.L., Fawell E.H., Armstrong J.
    Nucleic Acids Res. 18:7145-7145(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Osmoregulation of fission yeast: cloning of two distinct genes encoding glycerol-3-phosphate dehydrogenase, one of which is responsible for osmotolerance for growth."
    Ohmiya R., Yamada H., Nakashima K., Aiba H., Mizuno T.
    Mol. Microbiol. 18:963-973(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-376 AND THR-382, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiGPD1_SCHPO
AccessioniPrimary (citable) accession number: P21696
Secondary accession number(s): O94310, P78927
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 11, 2001
Last modified: October 29, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3