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P21696

- GPD1_SCHPO

UniProt

P21696 - GPD1_SCHPO

Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)] 1

Gene

gpd1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 2 (11 Jan 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei121 – 1211NADBy similarity
    Binding sitei144 – 1441NAD; via amide nitrogenBy similarity
    Binding sitei144 – 1441SubstrateBy similarity
    Binding sitei177 – 1771NAD; via amide nitrogenBy similarity
    Active sitei232 – 2321Proton acceptorBy similarity
    Binding sitei296 – 2961NADBy similarity
    Binding sitei325 – 3251NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi29 – 346NADBy similarity

    GO - Molecular functioni

    1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: PomBase
    2. NAD binding Source: InterPro

    GO - Biological processi

    1. cellular response to osmotic stress Source: PomBase
    2. cellular water homeostasis Source: PomBase
    3. glycerol-3-phosphate catabolic process Source: InterPro
    4. glycerol biosynthetic process Source: PomBase
    5. NADH oxidation Source: PomBase

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    ReactomeiREACT_201900. Synthesis of PA.
    REACT_224904. Triglyceride Biosynthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD(+)] 1 (EC:1.1.1.8)
    Alternative name(s):
    GPDH-C
    Short name:
    GPD-C
    Gene namesi
    Name:gpd1
    ORF Names:SPBC215.05
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC215.05.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. cytosol Source: PomBase
    3. glycerol-3-phosphate dehydrogenase complex Source: InterPro
    4. nucleus Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 385385Glycerol-3-phosphate dehydrogenase [NAD(+)] 1PRO_0000138095Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei376 – 3761Phosphoserine1 Publication
    Modified residuei382 – 3821Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP21696.
    PaxDbiP21696.

    Interactioni

    Protein-protein interaction databases

    BioGridi276983. 45 interactions.
    MINTiMINT-4687624.
    STRINGi4896.SPBC215.05-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP21696.
    SMRiP21696. Positions 24-371.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni296 – 2972Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0240.
    HOGENOMiHOG000246855.
    KOiK00006.
    OMAiCKLLSTS.
    OrthoDBiEOG7V76H7.
    PhylomeDBiP21696.

    Family and domain databases

    Gene3Di1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR017751. G3P_DH_NAD-dep_euk.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11728. PTHR11728. 1 hit.
    PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
    PRINTSiPR00077. GPDHDRGNASE.
    SUPFAMiSSF48179. SSF48179. 1 hit.
    TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
    PROSITEiPS00957. NAD_G3PDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21696-1 [UniParc]FASTAAdd to Basket

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    MSGYGQQGVS AANIDSIRPK KRLSIGVVGS GNWGTAIAKI CGENARAHGH    50
    HFRSKVRMWV FEEEIEYKGE KRKLTEVFNE AHENVKYLPG IECPPNVIAV 100
    PDVREVARRA DILVFVVPHQ FIERVCDQMV GLIRPGAVGI SCIKGVAVSK 150
    EGVRLYSEVI SEKLGIYCGV LSGANVANEV AREQFCETTI GFNPPNEVDI 200
    PREQIAAVFD RPYFSVVSVD DVAGVALGGA LKNVVAMAVG FADGLEWGGN 250
    TKAAIMRRGL LEMQKFATTF FDSDPRTMVE QSCGIADLVT SCLGGRNNRC 300
    AEAFVKTGKS LETLEKELLG GQLLQGAATS KDVHEFLLTK DMVKDFPLFT 350
    AVYNISYEDM DPKDLIIVLQ PLKEDSENEG GTETE 385
    Length:385
    Mass (Da):41,995
    Last modified:January 11, 2001 - v2
    Checksum:i8061C294196516E1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti54 – 563SKV → GKG in CAA39630. (PubMed:2263480)Curated
    Sequence conflicti126 – 1272CD → WH in CAA39630. (PubMed:2263480)Curated
    Sequence conflicti153 – 1531Missing in CAA39630. (PubMed:2263480)Curated
    Sequence conflicti154 – 1541R → S in CAA39630. (PubMed:2263480)Curated
    Sequence conflicti209 – 2091F → S in CAA39630. (PubMed:2263480)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56162 Genomic DNA. Translation: CAA39630.1.
    CU329671 Genomic DNA. Translation: CAA22119.1.
    D50796 Genomic DNA. Translation: BAA09424.1.
    PIRiT39895.
    RefSeqiNP_596682.1. NM_001022605.2.

    Genome annotation databases

    EnsemblFungiiSPBC215.05.1; SPBC215.05.1:pep; SPBC215.05.
    GeneIDi2540455.
    KEGGispo:SPBC215.05.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56162 Genomic DNA. Translation: CAA39630.1 .
    CU329671 Genomic DNA. Translation: CAA22119.1 .
    D50796 Genomic DNA. Translation: BAA09424.1 .
    PIRi T39895.
    RefSeqi NP_596682.1. NM_001022605.2.

    3D structure databases

    ProteinModelPortali P21696.
    SMRi P21696. Positions 24-371.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 276983. 45 interactions.
    MINTi MINT-4687624.
    STRINGi 4896.SPBC215.05-1.

    Proteomic databases

    MaxQBi P21696.
    PaxDbi P21696.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC215.05.1 ; SPBC215.05.1:pep ; SPBC215.05 .
    GeneIDi 2540455.
    KEGGi spo:SPBC215.05.

    Organism-specific databases

    PomBasei SPBC215.05.

    Phylogenomic databases

    eggNOGi COG0240.
    HOGENOMi HOG000246855.
    KOi K00006.
    OMAi CKLLSTS.
    OrthoDBi EOG7V76H7.
    PhylomeDBi P21696.

    Enzyme and pathway databases

    Reactomei REACT_201900. Synthesis of PA.
    REACT_224904. Triglyceride Biosynthesis.

    Miscellaneous databases

    NextBioi 20801582.

    Family and domain databases

    Gene3Di 1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR017751. G3P_DH_NAD-dep_euk.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11728. PTHR11728. 1 hit.
    Pfami PF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000114. Glycerol-3-P_dh. 1 hit.
    PRINTSi PR00077. GPDHDRGNASE.
    SUPFAMi SSF48179. SSF48179. 1 hit.
    TIGRFAMsi TIGR03376. glycerol3P_DH. 1 hit.
    PROSITEi PS00957. NAD_G3PDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Glycerol-3-phosphate dehydrogenase homologue from Schizosaccharomyces pombe."
      Pidoux A.L., Fawell E.H., Armstrong J.
      Nucleic Acids Res. 18:7145-7145(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "Osmoregulation of fission yeast: cloning of two distinct genes encoding glycerol-3-phosphate dehydrogenase, one of which is responsible for osmotolerance for growth."
      Ohmiya R., Yamada H., Nakashima K., Aiba H., Mizuno T.
      Mol. Microbiol. 18:963-973(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-376 AND THR-382, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiGPD1_SCHPO
    AccessioniPrimary (citable) accession number: P21696
    Secondary accession number(s): O94310, P78927
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: January 11, 2001
    Last modified: October 1, 2014
    This is version 126 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3