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Reviewed, UniProtKB/Swiss-Prot P21695 (GPDA_HUMAN)

Last modified November 3, 2009. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic
      Short name=GPDH-C
      Short name=GPD-C
    EC=1.1.1.8
Gene names
Name: GPD1
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Enzyme regulation

Inhibited by zinc ions and sulfate. Ref.4

Subunit structure

Homodimer. Ref.4

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 349348Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic
PRO_0000138079

Regions

Nucleotide binding10 – 156NAD
Region269 – 2702Substrate binding

Sites

Active site2041Proton acceptor Probable
Binding site411NAD
Binding site971NAD
Binding site1201Substrate
Binding site1531NAD; via amide nitrogen
Binding site2691NAD
Binding site2961NAD; via amide nitrogen
Binding site2981NAD

Natural variations

Natural variant541I → V: dbSNP rs2232202.
VAR_029492
Natural variant1131A → P: dbSNP rs1128867. Ref.1
VAR_029493
Natural variant1971V → A: dbSNP rs2232207.
VAR_049220

Secondary structure

1 ........................................................... 349
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P21695-1 [UniParc].

Last modified March 20, 2007. Version 4.
Checksum: CE7B7681103076EF

FASTA34937,568
        10         20         30         40         50         60 
MASKKVCIVG SGNWGSAIAK IVGGNAAQLA QFDPRVTMWV FEEDIGGKKL TEIINTQHEN 

        70         80         90        100        110        120 
VKYLPGHKLP PNVVAVPDVV QAAEDADILI FVVPHQFIGK ICDQLKGHLK ANATGISLIK 

       130        140        150        160        170        180 
GVDEGPNGLK LISEVIGERL GIPMSVLMGA NIASEVADEK FCETTIGCKD PAQGQLLKEL 

       190        200        210        220        230        240 
MQTPNFRITV VQEVDTVEIC GALKNVVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK 

       250        260        270        280        290        300 
LFCSGPVSSA TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE LLNGQKLQGP 

       310        320        330        340 
ETARELYSIL QHKGLVDKFP LFMAVYKVCY EGQPVGEFIH CLQNHPEHM

« Hide

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References

« Hide 'large scale' references
[1]"Molecular cloning, sequencing and expression of a cDNA encoding a human liver NAD-dependent alpha-glycerol-3-phosphate dehydrogenase."
Menaya J., Gonzalez-Manchon C., Parrilla R., Ayuso M.S.
Biochim. Biophys. Acta 1262:91-94(1995) [PubMed: 7772607] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT PRO-113.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pancreas.
[3]"Sequence conservation and structural organization of the glycerol-3-phosphate dehydrogenase promoter in mice and humans."
Gwynn B., Lyford K.L., Birkenmeier E.H.
Mol. Cell. Biol. 10:5244-5256(1990) [PubMed: 2398890] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
Tissue: Fetal liver.
[4]"Crystal structures of human glycerol 3-phosphate dehydrogenase 1 (GPD1)."
Ou X., Ji C., Han X., Zhao X., Li X., Mao Y., Wong L.-L., Bartlam M., Rao Z.
J. Mol. Biol. 357:858-869(2006) [PubMed: 16460752] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH NAD, SUBUNIT, ENZYME REGULATION.

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Cross-references

Sequence databases

L34041 mRNA. Translation: AAA92863.1.
BC032234 mRNA. Translation: AAH32234.1.
M36917 Genomic DNA. Translation: AAA35925.1.
IPIIPI00295777.
PIRS55920.
RefSeqNP_005267.2.
UniGeneHs.524418

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1WPQX-ray2.50A/B1-349[»]
1X0VX-ray2.30A/B1-349[»]
1X0XX-ray2.75A1-349[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP21695. 1 interaction.
STRINGP21695.

Proteomic databases

PRIDEP21695.

Genome annotation databases

EnsemblENST00000301149; ENSP00000301149; ENSG00000167588; Homo sapiens. [Genome view]
GeneID2819.
KEGGhsa:2819.
NMPDRfig|9606.3.peg.7531.
UCSCuc001rvz.1. human.

Organism-specific databases

CTD2819.
GeneCardsGC12P048784.
H-InvDBHIX0010622.
HGNCHGNC:4455. GPD1.
MIM138420. gene.
PharmGKBPA28836.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP21695.
HOVERGENP21695.
OMATHENVKY.

Enzyme and pathway databases

BRENDA1.1.1.8. 247.
ReactomeREACT_602. Metabolism of lipids and lipoproteins.

Gene expression databases

ArrayExpressP21695.
BgeeP21695.
CleanExHS_GPD1.
GenevestigatorP21695.
GermOnlineENSG00000167588. Homo sapiens.

Family and domain databases

InterProIPR013328. DH_multihelical.
IPR016040. NAD(P)-bd_dom.
IPR006168. NAD-dep_Gly3P_DH.
IPR017751. NAD-dep_Gly3P_DH_euk.
IPR011128. NAD-dep_Gly3P_DH_N.
IPR006109. NAD_Gly3P_DH_C.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHERPTHR11728. NAD_Gly3P_DH. 1 hit.
PfamPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSPR00077. GPDHDRGNASE.
ProDomPD001278. NAD_Gly3P_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR03376. glycerol3P_DH. 1 hit.
PROSITEPS00957. NAD_G3PDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

DrugBankDB00157. NADH.
NextBio11107.
SOURCESearch...

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Entry information

Entry nameGPDA_HUMAN
AccessionPrimary (citable) accession number: P21695
Secondary accession number(s): Q8N1B0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: March 20, 2007
Last modified: November 3, 2009
This is version 92 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents