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P21691

- SIR1_YEAST

UniProt

P21691 - SIR1_YEAST

Protein

Regulatory protein SIR1

Gene

SIR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Involved in the establishment, but not the maintenance, of heterochromatic silencing at the cryptic mating-type loci HMR and HML. Is recruited by interacting with the ORC1 subunit of the origin recognition complex (ORC), which binds to HML-I or HMR-E silencers, DNA elements that direct the formation of silent chromatin at the mating-type loci. Establishes transcriptional silencing by recruiting the three other SIR proteins, SIR2, SIR3, and SIR4, that function directly in silenced chromatin and establish repression. Also found in centromeric chromatin. Binds to and helps retain CAC1, a subunit of chromatin assembly factor I (CAF-I) at centromeric loci independent on the other SIR proteins.3 Publications

    GO - Molecular functioni

    1. chromatin binding Source: SGD
    2. sequence-specific DNA binding Source: SGD

    GO - Biological processi

    1. chromatin silencing at silent mating-type cassette Source: SGD
    2. establishment of chromatin silencing Source: SGD
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32063-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regulatory protein SIR1
    Alternative name(s):
    Heterochromatin protein SIR1
    Silent information regulator 1
    Gene namesi
    Name:SIR1
    Ordered Locus Names:YKR101W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKR101w.
    SGDiS000001809. SIR1.

    Subcellular locationi

    Nucleus. Chromosomecentromere
    Note: Associated primarily with the HMR-E silencer at the HMR locus.

    GO - Cellular componenti

    1. chromatin silencing complex Source: SGD
    2. chromosome, centromeric region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Centromere, Chromosome, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi462 – 4643EEE → AAA: No effect.
    Mutagenesisi466 – 4661V → G: Abolishes interaction with ORC1. 1 Publication
    Mutagenesisi469 – 4691R → G: Abolishes interaction with ORC1. 1 Publication
    Mutagenesisi470 – 4701F → S: Abolishes interaction with ORC1 and SIR4. 1 Publication
    Mutagenesisi477 – 4771L → P: Abolishes interaction with ORC1 and SIR4. 1 Publication
    Mutagenesisi479 – 4791D → N: Abolishes interaction with ORC1. 1 Publication
    Mutagenesisi480 – 4801L → P: Abolishes interaction with ORC1. 1 Publication
    Mutagenesisi482 – 4832EE → AA: No effect.
    Mutagenesisi489 – 4902KD → AA: No effect.
    Mutagenesisi498 – 4992KD → AA: No effect.
    Mutagenesisi513 – 5131W → R: Abolishes interaction with ORC1 and SIR4. 1 Publication
    Mutagenesisi517 – 5182KK → AA: No effect.
    Mutagenesisi534 – 5341C → A: No effect. 1 Publication
    Mutagenesisi538 – 5403KKK → AAA: Abolishes interaction with SIR4.
    Mutagenesisi569 – 5691C → A: No effect.
    Mutagenesisi571 – 5711C → R: Abolishes interaction with ORC1 and SIR4. 1 Publication
    Mutagenesisi572 – 5721V → G: No effect. 1 Publication
    Mutagenesisi573 – 5731P → S: No effect. 1 Publication
    Mutagenesisi577 – 5782DD → AA: Abolishes interaction with SIR4.
    Mutagenesisi584 – 5841L → P or Q: Abolishes interaction with ORC1 and SIR4. 1 Publication
    Mutagenesisi586 – 5872DD → AA: Abolishes interaction with SIR4.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 654654Regulatory protein SIR1PRO_0000097767Add
    BLAST

    Proteomic databases

    MaxQBiP21691.

    Expressioni

    Gene expression databases

    GenevestigatoriP21691.

    Interactioni

    Subunit structurei

    Interacts (via OIR domain) with ORC1 (via BAH domain). Interacts with SIR4. Interacts with CAC1.6 Publications

    Protein-protein interaction databases

    BioGridi34232. 70 interactions.
    DIPiDIP-2453N.
    IntActiP21691. 3 interactions.
    MINTiMINT-518571.
    STRINGi4932.YKR101W.

    Structurei

    Secondary structure

    1
    654
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi464 – 47310
    Beta strandi476 – 4794
    Turni480 – 4834
    Beta strandi484 – 4863
    Helixi493 – 4964
    Helixi499 – 5068
    Helixi513 – 5164
    Beta strandi523 – 5253
    Helixi530 – 5334
    Beta strandi537 – 5437
    Beta strandi547 – 5504
    Beta strandi566 – 57510
    Beta strandi577 – 5793
    Beta strandi581 – 5866

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Z1AX-ray2.50A/B449-587[»]
    1ZBXX-ray2.50B456-588[»]
    1ZHIX-ray2.70B456-587[»]
    ProteinModelPortaliP21691.
    SMRiP21691. Positions 463-587.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP21691.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni322 – 654333Sufficient for interaction with SIR4Add
    BLAST
    Regioni449 – 587139ORC interacting region (OIR)Add
    BLAST

    Phylogenomic databases

    eggNOGiNOG281951.
    KOiK11120.
    OMAiVENTISN.
    OrthoDBiEOG7Z0K50.

    Family and domain databases

    InterProiIPR021646. Regulatory_Sir1.
    [Graphical view]
    PfamiPF11603. Sir1. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21691-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLQINSRLAV IDGWLVDTVK RKPINFRSPE VRLLLPNDDD YKKLSQQNLV    50
    DWTRLKKDSN SVLVGVKSME LFKHIKLVLR EFFLLEDGRI ILKRIRSKLR 100
    YKVVKKLTCK CCRLYLPKWG TVYIHPMLKD KEKPLAGVCE FSLDVNPDRE 150
    YPLIEINVSH QYIIIEGFLL YLNERRLYRW NDNNLRSQVG LTKWAHLRKT 200
    YNPVSLDILY SLNSNFYFVK DDLLFQLLGK RVFVKFCKVM ENGKCGKAPL 250
    WYRVKRTTTA KATHIAYAIS NSTAPDSFKS KNNDYRFIVR EKPIVENTIS 300
    NLDYSDIKKQ QFTEAEVVKR KISADISQIE NVHTQFNSQK EKNNIRVNKV 350
    SSEVLDQISK FPVSRVTLLL MSAGQDKNYI ELVEELARRL EKICIEKTTQ 400
    SLEEIRDTFQ ANPEMQASFD KEYYQSIEEY KITLELIKED LLITLIKQME 450
    NMWAAEKKFS TEEEYVSPRF LVADGFLIDL AEEKPINPKD PRLLTLLKDH 500
    QRAMIDQMNL VKWNDFKKYQ DPIPLKAKTL FKFCKQIKKK FLRGADFKLH 550
    TLPTEANLKY EPERMTVLCS CVPILLDDQT VQYLYDDSII PEFEATSSYA 600
    TKQSKCGRKM SLQMEPDLLF QEAIRRMRHL TAYDVLRRNY IAAFEELYMG 650
    NCND 654
    Length:654
    Mass (Da):76,933
    Last modified:July 28, 2009 - v2
    Checksum:i00FEABD1BD35BC81
    GO

    Sequence cautioni

    The sequence AAA35046.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA82181.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M38524 Genomic DNA. Translation: AAA35046.1. Different initiation.
    Z28326 Genomic DNA. Translation: CAA82181.1. Different initiation.
    BK006944 Genomic DNA. Translation: DAA09252.1.
    PIRiS14173.
    RefSeqiNP_013027.4. NM_001179891.3.

    Genome annotation databases

    EnsemblFungiiYKR101W; YKR101W; YKR101W.
    GeneIDi853976.
    KEGGisce:YKR101W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M38524 Genomic DNA. Translation: AAA35046.1 . Different initiation.
    Z28326 Genomic DNA. Translation: CAA82181.1 . Different initiation.
    BK006944 Genomic DNA. Translation: DAA09252.1 .
    PIRi S14173.
    RefSeqi NP_013027.4. NM_001179891.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Z1A X-ray 2.50 A/B 449-587 [» ]
    1ZBX X-ray 2.50 B 456-588 [» ]
    1ZHI X-ray 2.70 B 456-587 [» ]
    ProteinModelPortali P21691.
    SMRi P21691. Positions 463-587.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34232. 70 interactions.
    DIPi DIP-2453N.
    IntActi P21691. 3 interactions.
    MINTi MINT-518571.
    STRINGi 4932.YKR101W.

    Proteomic databases

    MaxQBi P21691.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKR101W ; YKR101W ; YKR101W .
    GeneIDi 853976.
    KEGGi sce:YKR101W.

    Organism-specific databases

    CYGDi YKR101w.
    SGDi S000001809. SIR1.

    Phylogenomic databases

    eggNOGi NOG281951.
    KOi K11120.
    OMAi VENTISN.
    OrthoDBi EOG7Z0K50.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32063-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P21691.
    NextBioi 975431.

    Gene expression databases

    Genevestigatori P21691.

    Family and domain databases

    InterProi IPR021646. Regulatory_Sir1.
    [Graphical view ]
    Pfami PF11603. Sir1. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The SIR1 gene of Saccharomyces cerevisiae and its role as an extragenic suppressor of several mating-defective mutants."
      Stone E.M., Swanson M.J., Romeo A.M., Hicks J.B., Sternglanz R.
      Mol. Cell. Biol. 11:2253-2262(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Targeting of SIR1 protein establishes transcriptional silencing at HM loci and telomeres in yeast."
      Chien C.T., Buck S., Sternglanz R., Shore D.
      Cell 75:531-541(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Role of interactions between the origin recognition complex and SIR1 in transcriptional silencing."
      Triolo T., Sternglanz R.
      Nature 381:251-253(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ORC1.
    6. "The molecular biology of the SIR proteins."
      Gasser S.M., Cockell M.M.
      Gene 279:1-16(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    7. "The Sir1 protein's association with a silenced chromosome domain."
      Gardner K.A., Fox C.A.
      Genes Dev. 15:147-157(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    8. "Structure and function of the BAH-containing domain of Orc1p in epigenetic silencing."
      Zhang Z., Hayashi M.K., Merkel O., Stillman B., Xu R.-M.
      EMBO J. 21:4600-4611(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ORC1.
    9. "Ordered nucleation and spreading of silenced chromatin in Saccharomyces cerevisiae."
      Rusche L.N., Kirchmaier A.L., Rine J.
      Mol. Biol. Cell 13:2207-2222(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    10. "The budding yeast silencing protein Sir1 is a functional component of centromeric chromatin."
      Sharp J.A., Krawitz D.C., Gardner K.A., Fox C.A., Kaufman P.D.
      Genes Dev. 17:2356-2361(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CAC1.
    11. "The origin recognition complex and Sir4 protein recruit Sir1p to yeast silent chromatin through independent interactions requiring a common Sir1p domain."
      Bose M.E., McConnell K.H., Gardner-Aukema K.A., Mueller U., Weinreich M., Keck J.L., Fox C.A.
      Mol. Cell. Biol. 24:774-786(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ORC1 AND SIR4, MUTAGENESIS OF 462-GLU--GLU-464; VAL-466; ARG-469; PHE-470; LEU-477; ASP-479; LEU-480; 482-GLU-GLU-483; 489-LYS-ASP-490; 498-LYS-ASP-499; TRP-513; 517-LYS-LYS-518; CYS-534; 538-LYS--LYS-540; CYS-571; VAL-572; PRO-573; 577-ASP-ASP-578; LEU-584 AND 586-ASP-ASP-587.
    12. "Elaboration, diversification and regulation of the sir1 family of silencing proteins in Saccharomyces."
      Gallagher J.E.G., Babiarz J.E., Teytelman L., Wolfe K.H., Rine J.
      Genetics 181:1477-1491(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF INITIATION SITE.
    13. "Structural basis of the Sir1-origin recognition complex interaction in transcriptional silencing."
      Hou Z., Bernstein D.A., Fox C.A., Keck J.L.
      Proc. Natl. Acad. Sci. U.S.A. 102:8489-8494(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 449-587 OF MUTANT ALA-569 IN COMPLEX WITH ORC1.
    14. "Structural basis for origin recognition complex 1 protein-silence information regulator 1 protein interaction in epigenetic silencing."
      Hsu H.-C., Stillman B., Xu R.-M.
      Proc. Natl. Acad. Sci. U.S.A. 102:8519-8524(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 456-588 IN COMPLEX WITH ORC1.

    Entry informationi

    Entry nameiSIR1_YEAST
    AccessioniPrimary (citable) accession number: P21691
    Secondary accession number(s): D6VXG2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3