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P21685 (CRTI_PANAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phytoene desaturase (lycopene-forming)
EC=1.3.99.31
Alternative name(s):
4-step phytoene desaturase
Phytoene dehydrogenase
Gene names
Name:crtI
OrganismPantoea ananas (Erwinia uredovora)
Taxonomic identifier553 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pantoea

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Converts 15-cis-phytoene into all-trans-lycopene via the intermediary of all-trans-phytofluene, all-trans-zeta-carotene and all-trans-neurosporene, by the introduction of four double bonds. Ref.2

Catalytic activity

15-cis-phytoene + 4 acceptor = all-trans-lycopene + 4 reduced acceptor. Ref.2

Cofactor

FAD. Ref.2

Enzyme regulation

Inhibited by NAD and NADP. Ref.2

Pathway

Carotenoid biosynthesis; lycopene biosynthesis. Ref.1 Ref.2

Subcellular location

Cell membrane Ref.2.

Sequence similarities

Belongs to the carotenoid/retinoid oxidoreductase family.

Ontologies

Keywords
   Biological processCarotenoid biosynthesis
   Cellular componentCell membrane
Membrane
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processcarotene biosynthetic process

Inferred from direct assay Ref.2. Source: UniProtKB

carotenoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionFAD binding

Inferred from direct assay Ref.2. Source: UniProtKB

oxidoreductase activity, acting on the CH-CH group of donors

Inferred from direct assay Ref.2. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 492492Phytoene desaturase (lycopene-forming)
PRO_0000067686

Regions

Nucleotide binding5 – 3834FAD Potential

Secondary structure

1 ............................................................................ 492
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P21685 [UniParc].

Last modified May 1, 1991. Version 1.
Checksum: FA4CD4E34A9C6413

FASTA49255,008
        10         20         30         40         50         60 
MKPTTVIGAG FGGLALAIRL QAAGIPVLLL EQRDKPGGRA YVYEDQGFTF DAGPTVITDP 

        70         80         90        100        110        120 
SAIEELFALA GKQLKEYVEL LPVTPFYRLC WESGKVFNYD NDQTRLEAQI QQFNPRDVEG 

       130        140        150        160        170        180 
YRQFLDYSRA VFKEGYLKLG TVPFLSFRDM LRAAPQLAKL QAWRSVYSKV ASYIEDEHLR 

       190        200        210        220        230        240 
QAFSFHSLLV GGNPFATSSI YTLIHALERE WGVWFPRGGT GALVQGMIKL FQDLGGEVVL 

       250        260        270        280        290        300 
NARVSHMETT GNKIEAVHLE DGRRFLTQAV ASNADVVHTY RDLLSQHPAA VKQSNKLQTK 

       310        320        330        340        350        360 
RMSNSLFVLY FGLNHHHDQL AHHTVCFGPR YRELIDEIFN HDGLAEDFSL YLHAPCVTDS 

       370        380        390        400        410        420 
SLAPEGCGSY YVLAPVPHLG TANLDWTVEG PKLRDRIFAY LEQHYMPGLR SQLVTHRMFT 

       430        440        450        460        470        480 
PFDFRDQLNA YHGSAFSVEP VLTQSAWFRP HNRDKTITNL YLVGAGTHPG AGIPGVIGSA 

       490 
KATAGLMLED LI

« Hide

References

[1]"Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli."
Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., Nakamura K., Harashima K.
J. Bacteriol. 172:6704-6712(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PATHWAY.
Strain: 20D3.
[2]"Expression in Escherichia coli, purification, and reactivation of the recombinant Erwinia uredovora phytoene desaturase."
Fraser P.D., Misawa N., Linden H., Yamano S., Kobayashi K., Sandmann G.
J. Biol. Chem. 267:19891-19895(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, PATHWAY, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D90087 Genomic DNA. Translation: BAA14127.1.
PIRD37802.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4DGKX-ray2.35A1-492[»]
ProteinModelPortalP21685.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-15564.
UniPathwayUPA00803.

Family and domain databases

InterProIPR002937. Amino_oxidase.
IPR014105. CrtI_fam.
IPR008150. Phytoene_DH_bac_CS.
[Graphical view]
PANTHERPTHR10668:SF3. PTHR10668:SF3. 1 hit.
PfamPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsTIGR02734. crtI_fam. 1 hit.
PROSITEPS00982. PHYTOENE_DH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCRTI_PANAN
AccessionPrimary (citable) accession number: P21685
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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