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P21685

- CRTI_PANAN

UniProt

P21685 - CRTI_PANAN

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Protein

Phytoene desaturase (lycopene-forming)

Gene

crtI

Organism
Pantoea ananas (Erwinia uredovora)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Converts 15-cis-phytoene into all-trans-lycopene via the intermediary of all-trans-phytofluene, all-trans-zeta-carotene and all-trans-neurosporene, by the introduction of four double bonds.1 Publication

Catalytic activityi

15-cis-phytoene + 4 acceptor = all-trans-lycopene + 4 reduced acceptor.1 Publication

Cofactori

FAD.1 Publication

Enzyme regulationi

Inhibited by NAD and NADP.1 Publication

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi5 – 3834FADSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. FAD binding Source: UniProtKB
  2. oxidoreductase activity, acting on the CH-CH group of donors Source: UniProtKB

GO - Biological processi

  1. carotene biosynthetic process Source: UniProtKB
  2. carotenoid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carotenoid biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15564.
UniPathwayiUPA00803.

Names & Taxonomyi

Protein namesi
Recommended name:
Phytoene desaturase (lycopene-forming) (EC:1.3.99.31)
Alternative name(s):
4-step phytoene desaturase
Phytoene dehydrogenase
Gene namesi
Name:crtI
OrganismiPantoea ananas (Erwinia uredovora)
Taxonomic identifieri553 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePantoea

Subcellular locationi

Cell membrane 1 Publication

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492Phytoene desaturase (lycopene-forming)PRO_0000067686Add
BLAST

Structurei

Secondary structure

1
492
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74
Helixi10 – 2213
Beta strandi27 – 304
Beta strandi42 – 454
Beta strandi48 – 514
Helixi61 – 688
Turni69 – 713
Helixi74 – 763
Beta strandi80 – 9112
Beta strandi96 – 994
Helixi103 – 11311
Helixi117 – 13115
Beta strandi133 – 1353
Helixi147 – 1526
Helixi154 – 1563
Turni157 – 1593
Helixi161 – 17111
Helixi177 – 19115
Helixi201 – 2088
Beta strandi213 – 2164
Helixi219 – 23315
Beta strandi237 – 2393
Beta strandi244 – 2507
Beta strandi253 – 2597
Beta strandi264 – 2663
Beta strandi270 – 2723
Beta strandi305 – 31511
Beta strandi322 – 3287
Beta strandi347 – 3537
Helixi355 – 3573
Helixi360 – 3623
Beta strandi368 – 3769
Turni379 – 3813
Helixi386 – 40419
Helixi409 – 4124
Beta strandi413 – 4197
Turni421 – 4244
Beta strandi460 – 4623
Helixi472 – 49120

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DGKX-ray2.35A1-492[»]
ProteinModelPortaliP21685.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR002937. Amino_oxidase.
IPR014105. CrtI_fam.
IPR008150. Phytoene_DH_bac_CS.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02734. crtI_fam. 1 hit.
PROSITEiPS00982. PHYTOENE_DH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21685-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKPTTVIGAG FGGLALAIRL QAAGIPVLLL EQRDKPGGRA YVYEDQGFTF
60 70 80 90 100
DAGPTVITDP SAIEELFALA GKQLKEYVEL LPVTPFYRLC WESGKVFNYD
110 120 130 140 150
NDQTRLEAQI QQFNPRDVEG YRQFLDYSRA VFKEGYLKLG TVPFLSFRDM
160 170 180 190 200
LRAAPQLAKL QAWRSVYSKV ASYIEDEHLR QAFSFHSLLV GGNPFATSSI
210 220 230 240 250
YTLIHALERE WGVWFPRGGT GALVQGMIKL FQDLGGEVVL NARVSHMETT
260 270 280 290 300
GNKIEAVHLE DGRRFLTQAV ASNADVVHTY RDLLSQHPAA VKQSNKLQTK
310 320 330 340 350
RMSNSLFVLY FGLNHHHDQL AHHTVCFGPR YRELIDEIFN HDGLAEDFSL
360 370 380 390 400
YLHAPCVTDS SLAPEGCGSY YVLAPVPHLG TANLDWTVEG PKLRDRIFAY
410 420 430 440 450
LEQHYMPGLR SQLVTHRMFT PFDFRDQLNA YHGSAFSVEP VLTQSAWFRP
460 470 480 490
HNRDKTITNL YLVGAGTHPG AGIPGVIGSA KATAGLMLED LI
Length:492
Mass (Da):55,008
Last modified:May 1, 1991 - v1
Checksum:iFA4CD4E34A9C6413
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D90087 Genomic DNA. Translation: BAA14127.1.
PIRiD37802.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D90087 Genomic DNA. Translation: BAA14127.1 .
PIRi D37802.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4DGK X-ray 2.35 A 1-492 [» ]
ProteinModelPortali P21685.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00803 .
BioCyci MetaCyc:MONOMER-15564.

Family and domain databases

InterProi IPR002937. Amino_oxidase.
IPR014105. CrtI_fam.
IPR008150. Phytoene_DH_bac_CS.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02734. crtI_fam. 1 hit.
PROSITEi PS00982. PHYTOENE_DH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli."
    Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., Nakamura K., Harashima K.
    J. Bacteriol. 172:6704-6712(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PATHWAY.
    Strain: 20D3.
  2. "Expression in Escherichia coli, purification, and reactivation of the recombinant Erwinia uredovora phytoene desaturase."
    Fraser P.D., Misawa N., Linden H., Yamano S., Kobayashi K., Sandmann G.
    J. Biol. Chem. 267:19891-19895(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, PATHWAY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCRTI_PANAN
AccessioniPrimary (citable) accession number: P21685
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3