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Protein

Phytoene desaturase (lycopene-forming)

Gene

crtI

Organism
Pantoea ananas (Erwinia uredovora)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Converts 15-cis-phytoene into all-trans-lycopene via the intermediary of all-trans-phytofluene, all-trans-zeta-carotene and all-trans-neurosporene, by the introduction of four double bonds.1 Publication

Catalytic activityi

15-cis-phytoene + 4 acceptor = all-trans-lycopene + 4 reduced acceptor.1 Publication

Cofactori

FAD1 Publication

Enzyme regulationi

Inhibited by NAD and NADP.1 Publication

Pathwayi: lycopene biosynthesis

This protein is involved in the pathway lycopene biosynthesis, which is part of Carotenoid biosynthesis.2 Publications
View all proteins of this organism that are known to be involved in the pathway lycopene biosynthesis and in Carotenoid biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi5 – 38FADSequence analysisAdd BLAST34

GO - Molecular functioni

  • FAD binding Source: UniProtKB
  • oxidoreductase activity, acting on the CH-CH group of donors Source: UniProtKB

GO - Biological processi

  • carotene biosynthetic process Source: UniProtKB
  • carotenoid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carotenoid biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15564.
BRENDAi1.3.99.31. 2137.
UniPathwayiUPA00803.

Names & Taxonomyi

Protein namesi
Recommended name:
Phytoene desaturase (lycopene-forming) (EC:1.3.99.31)
Alternative name(s):
4-step phytoene desaturase
Phytoene dehydrogenase
Gene namesi
Name:crtI
OrganismiPantoea ananas (Erwinia uredovora)
Taxonomic identifieri553 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaePantoea

Subcellular locationi

  • Cell membrane 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000676861 – 492Phytoene desaturase (lycopene-forming)Add BLAST492

Interactioni

Protein-protein interaction databases

STRINGi706191.PANA_4161.

Structurei

Secondary structure

1492
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 7Combined sources4
Helixi10 – 22Combined sources13
Beta strandi27 – 30Combined sources4
Beta strandi42 – 45Combined sources4
Beta strandi48 – 51Combined sources4
Helixi61 – 68Combined sources8
Turni69 – 71Combined sources3
Helixi74 – 76Combined sources3
Beta strandi80 – 91Combined sources12
Beta strandi96 – 99Combined sources4
Helixi103 – 113Combined sources11
Helixi117 – 131Combined sources15
Beta strandi133 – 135Combined sources3
Helixi147 – 152Combined sources6
Helixi154 – 156Combined sources3
Turni157 – 159Combined sources3
Helixi161 – 171Combined sources11
Helixi177 – 191Combined sources15
Helixi201 – 208Combined sources8
Beta strandi213 – 216Combined sources4
Helixi219 – 233Combined sources15
Beta strandi237 – 239Combined sources3
Beta strandi244 – 250Combined sources7
Beta strandi253 – 259Combined sources7
Beta strandi264 – 266Combined sources3
Beta strandi270 – 272Combined sources3
Beta strandi305 – 315Combined sources11
Beta strandi322 – 328Combined sources7
Beta strandi347 – 353Combined sources7
Helixi355 – 357Combined sources3
Helixi360 – 362Combined sources3
Beta strandi368 – 376Combined sources9
Turni379 – 381Combined sources3
Helixi386 – 404Combined sources19
Helixi409 – 412Combined sources4
Beta strandi413 – 419Combined sources7
Turni421 – 424Combined sources4
Beta strandi460 – 462Combined sources3
Helixi472 – 491Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DGKX-ray2.35A1-492[»]
ProteinModelPortaliP21685.
SMRiP21685.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105EWK. Bacteria.
COG1233. LUCA.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR002937. Amino_oxidase.
IPR014105. Carotenoid/retinoid_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR008150. Phytoene_DH_bac_CS.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR02734. crtI_fam. 1 hit.
PROSITEiPS00982. PHYTOENE_DH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21685-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPTTVIGAG FGGLALAIRL QAAGIPVLLL EQRDKPGGRA YVYEDQGFTF
60 70 80 90 100
DAGPTVITDP SAIEELFALA GKQLKEYVEL LPVTPFYRLC WESGKVFNYD
110 120 130 140 150
NDQTRLEAQI QQFNPRDVEG YRQFLDYSRA VFKEGYLKLG TVPFLSFRDM
160 170 180 190 200
LRAAPQLAKL QAWRSVYSKV ASYIEDEHLR QAFSFHSLLV GGNPFATSSI
210 220 230 240 250
YTLIHALERE WGVWFPRGGT GALVQGMIKL FQDLGGEVVL NARVSHMETT
260 270 280 290 300
GNKIEAVHLE DGRRFLTQAV ASNADVVHTY RDLLSQHPAA VKQSNKLQTK
310 320 330 340 350
RMSNSLFVLY FGLNHHHDQL AHHTVCFGPR YRELIDEIFN HDGLAEDFSL
360 370 380 390 400
YLHAPCVTDS SLAPEGCGSY YVLAPVPHLG TANLDWTVEG PKLRDRIFAY
410 420 430 440 450
LEQHYMPGLR SQLVTHRMFT PFDFRDQLNA YHGSAFSVEP VLTQSAWFRP
460 470 480 490
HNRDKTITNL YLVGAGTHPG AGIPGVIGSA KATAGLMLED LI
Length:492
Mass (Da):55,008
Last modified:May 1, 1991 - v1
Checksum:iFA4CD4E34A9C6413
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90087 Genomic DNA. Translation: BAA14127.1.
PIRiD37802.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90087 Genomic DNA. Translation: BAA14127.1.
PIRiD37802.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DGKX-ray2.35A1-492[»]
ProteinModelPortaliP21685.
SMRiP21685.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi706191.PANA_4161.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105EWK. Bacteria.
COG1233. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00803.
BioCyciMetaCyc:MONOMER-15564.
BRENDAi1.3.99.31. 2137.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR002937. Amino_oxidase.
IPR014105. Carotenoid/retinoid_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR008150. Phytoene_DH_bac_CS.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR02734. crtI_fam. 1 hit.
PROSITEiPS00982. PHYTOENE_DH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRTI_PANAN
AccessioniPrimary (citable) accession number: P21685
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.