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P21685

- CRTI_PANAN

UniProt

P21685 - CRTI_PANAN

Protein

Phytoene desaturase (lycopene-forming)

Gene

crtI

Organism
Pantoea ananas (Erwinia uredovora)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 May 1991)
      Previous versions | rss
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    Functioni

    Converts 15-cis-phytoene into all-trans-lycopene via the intermediary of all-trans-phytofluene, all-trans-zeta-carotene and all-trans-neurosporene, by the introduction of four double bonds.1 Publication

    Catalytic activityi

    15-cis-phytoene + 4 acceptor = all-trans-lycopene + 4 reduced acceptor.1 Publication

    Cofactori

    FAD.1 Publication

    Enzyme regulationi

    Inhibited by NAD and NADP.1 Publication

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi5 – 3834FADSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. FAD binding Source: UniProtKB
    2. oxidoreductase activity, acting on the CH-CH group of donors Source: UniProtKB

    GO - Biological processi

    1. carotene biosynthetic process Source: UniProtKB
    2. carotenoid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carotenoid biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15564.
    UniPathwayiUPA00803.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phytoene desaturase (lycopene-forming) (EC:1.3.99.31)
    Alternative name(s):
    4-step phytoene desaturase
    Phytoene dehydrogenase
    Gene namesi
    Name:crtI
    OrganismiPantoea ananas (Erwinia uredovora)
    Taxonomic identifieri553 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePantoea

    Subcellular locationi

    Cell membrane 1 Publication

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 492492Phytoene desaturase (lycopene-forming)PRO_0000067686Add
    BLAST

    Structurei

    Secondary structure

    1
    492
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 74
    Helixi10 – 2213
    Beta strandi27 – 304
    Beta strandi42 – 454
    Beta strandi48 – 514
    Helixi61 – 688
    Turni69 – 713
    Helixi74 – 763
    Beta strandi80 – 9112
    Beta strandi96 – 994
    Helixi103 – 11311
    Helixi117 – 13115
    Beta strandi133 – 1353
    Helixi147 – 1526
    Helixi154 – 1563
    Turni157 – 1593
    Helixi161 – 17111
    Helixi177 – 19115
    Helixi201 – 2088
    Beta strandi213 – 2164
    Helixi219 – 23315
    Beta strandi237 – 2393
    Beta strandi244 – 2507
    Beta strandi253 – 2597
    Beta strandi264 – 2663
    Beta strandi270 – 2723
    Beta strandi305 – 31511
    Beta strandi322 – 3287
    Beta strandi347 – 3537
    Helixi355 – 3573
    Helixi360 – 3623
    Beta strandi368 – 3769
    Turni379 – 3813
    Helixi386 – 40419
    Helixi409 – 4124
    Beta strandi413 – 4197
    Turni421 – 4244
    Beta strandi460 – 4623
    Helixi472 – 49120

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4DGKX-ray2.35A1-492[»]
    ProteinModelPortaliP21685.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR002937. Amino_oxidase.
    IPR014105. CrtI_fam.
    IPR008150. Phytoene_DH_bac_CS.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02734. crtI_fam. 1 hit.
    PROSITEiPS00982. PHYTOENE_DH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21685-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKPTTVIGAG FGGLALAIRL QAAGIPVLLL EQRDKPGGRA YVYEDQGFTF    50
    DAGPTVITDP SAIEELFALA GKQLKEYVEL LPVTPFYRLC WESGKVFNYD 100
    NDQTRLEAQI QQFNPRDVEG YRQFLDYSRA VFKEGYLKLG TVPFLSFRDM 150
    LRAAPQLAKL QAWRSVYSKV ASYIEDEHLR QAFSFHSLLV GGNPFATSSI 200
    YTLIHALERE WGVWFPRGGT GALVQGMIKL FQDLGGEVVL NARVSHMETT 250
    GNKIEAVHLE DGRRFLTQAV ASNADVVHTY RDLLSQHPAA VKQSNKLQTK 300
    RMSNSLFVLY FGLNHHHDQL AHHTVCFGPR YRELIDEIFN HDGLAEDFSL 350
    YLHAPCVTDS SLAPEGCGSY YVLAPVPHLG TANLDWTVEG PKLRDRIFAY 400
    LEQHYMPGLR SQLVTHRMFT PFDFRDQLNA YHGSAFSVEP VLTQSAWFRP 450
    HNRDKTITNL YLVGAGTHPG AGIPGVIGSA KATAGLMLED LI 492
    Length:492
    Mass (Da):55,008
    Last modified:May 1, 1991 - v1
    Checksum:iFA4CD4E34A9C6413
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90087 Genomic DNA. Translation: BAA14127.1.
    PIRiD37802.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90087 Genomic DNA. Translation: BAA14127.1 .
    PIRi D37802.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4DGK X-ray 2.35 A 1-492 [» ]
    ProteinModelPortali P21685.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00803 .
    BioCyci MetaCyc:MONOMER-15564.

    Family and domain databases

    InterProi IPR002937. Amino_oxidase.
    IPR014105. CrtI_fam.
    IPR008150. Phytoene_DH_bac_CS.
    [Graphical view ]
    Pfami PF01593. Amino_oxidase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02734. crtI_fam. 1 hit.
    PROSITEi PS00982. PHYTOENE_DH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli."
      Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., Nakamura K., Harashima K.
      J. Bacteriol. 172:6704-6712(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PATHWAY.
      Strain: 20D3.
    2. "Expression in Escherichia coli, purification, and reactivation of the recombinant Erwinia uredovora phytoene desaturase."
      Fraser P.D., Misawa N., Linden H., Yamano S., Kobayashi K., Sandmann G.
      J. Biol. Chem. 267:19891-19895(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, PATHWAY, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiCRTI_PANAN
    AccessioniPrimary (citable) accession number: P21685
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: May 1, 1991
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3