P21683 (CRTB_PANAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phytoene synthase
EC=2.5.1.32

Gene names

Name:

crtB

Organism

Pantoea ananas (Erwinia uredovora)

Taxonomic identifier

553 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pantoea

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reaction from prephytoene diphosphate to phytoene.
Catalytic activity	2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.
Pathway	Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.
Sequence similarities	Belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis
Molecular function	Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	geranylgeranyl-diphosphate geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Phytoene synthase		PRO_0000067429

Sequences

	Sequence		Length	Mass (Da)
	P21683 [UniParc]. Last modified May 16, 2003. Version 2. Checksum: 9AA381A7376BBFC9	FASTA	309	34,472
	10 20 30 40 50 60 MNNPSLLNHA VETMAVGSKS FATASKLFDA KTRRSVLMLY AWCRHCDDVI DDQTLGFQAR 70 80 90 100 110 120 QPALQTPEQR LMQLEMKTRQ AYAGSQMHEP AFAAFQEVAM AHDIAPAYAF DHLEGFAMDV 130 140 150 160 170 180 REAQYSQLDD TLRYCYHVAG VVGLMMAQIM GVRDNATLDR ACDLGLAFQL TNIARDIVDD 190 200 210 220 230 240 AHAGRCYLPA SWLEHEGLNK ENYAAPENRQ ALSRIARRLV QEAEPYYLSA TAGLAGLPLR 250 260 270 280 290 300 SAWAIATAKQ VYRKIGVKVE QAGQQAWDQR QSTTTPEKLT LLLAASGQAL TSRMRAHPPR PAHLWQRPL

References

[1]	"Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli." Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., Nakamura K., Harashima K. J. Bacteriol. 172:6704-6712(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 20D3.
[2]	Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., Nakamura K., Harashima K. Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO N-TERMINUS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D90087 Genomic DNA. Translation: BAA14128.2.
PIR	E37802.
3D structure databases
ProteinModelPortal	P21683.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00799; UER00773.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR002060. Squ/phyt_synthse. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CRTB_PANAN

Accession

Primary (citable) accession number: P21683

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 16, 2003
Last modified:	April 3, 2013
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program