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P21683 (CRTB_PANAN) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
All-trans-phytoene synthase/15-cis-phytoene synthase

Short name=PSase
EC=2.5.1.32
EC=2.5.1.99
Gene names
Name:crtB
OrganismPantoea ananas (Erwinia uredovora)
Taxonomic identifier553 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePantoea

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the biosynthesis of carotenoids. Catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to yield both 15-cis and all-trans phytoene isomers. Ref.3

Catalytic activity

2 geranylgeranyl diphosphate = all-trans-phytoene + 2 diphosphate. Ref.3

2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate. Ref.3

Cofactor

ATP. ATP is required for the transferase activity but it does not seem to be hydrolyzed during the reaction. Ref.3

Manganese or magnesium. Ref.3

Enzyme regulation

Inhibited by phosphate ions and squalestatin. Ref.3

Pathway

Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.

Sequence similarities

Belongs to the phytoene/squalene synthase family.

Biophysicochemical properties

Kinetic parameters:

KM=41 µM for GGPP (at pH 8 and 37 degrees Celsius) Ref.3

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309All-trans-phytoene synthase/15-cis-phytoene synthase
PRO_0000067429

Sequences

Sequence LengthMass (Da)Tools
P21683 [UniParc].

Last modified May 16, 2003. Version 2.
Checksum: 9AA381A7376BBFC9

FASTA30934,472
        10         20         30         40         50         60 
MNNPSLLNHA VETMAVGSKS FATASKLFDA KTRRSVLMLY AWCRHCDDVI DDQTLGFQAR 

        70         80         90        100        110        120 
QPALQTPEQR LMQLEMKTRQ AYAGSQMHEP AFAAFQEVAM AHDIAPAYAF DHLEGFAMDV 

       130        140        150        160        170        180 
REAQYSQLDD TLRYCYHVAG VVGLMMAQIM GVRDNATLDR ACDLGLAFQL TNIARDIVDD 

       190        200        210        220        230        240 
AHAGRCYLPA SWLEHEGLNK ENYAAPENRQ ALSRIARRLV QEAEPYYLSA TAGLAGLPLR 

       250        260        270        280        290        300 
SAWAIATAKQ VYRKIGVKVE QAGQQAWDQR QSTTTPEKLT LLLAASGQAL TSRMRAHPPR 


PAHLWQRPL 

« Hide

References

[1]"Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli."
Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., Nakamura K., Harashima K.
J. Bacteriol. 172:6704-6712(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 20D3.
[2]Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., Nakamura K., Harashima K.
Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO N-TERMINUS.
[3]"Expression of an active phytoene synthase from Erwinia uredovora and biochemical properties of the enzyme."
Neudert U., Martinez-Ferez I.M., Fraser P.D., Sandmann G.
Biochim. Biophys. Acta 1392:51-58(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, COFACTOR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D90087 Genomic DNA. Translation: BAA14128.2.
PIRE37802.

3D structure databases

ProteinModelPortalP21683.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00799; UER00773.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
InterProIPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMSSF48576. SSF48576. 1 hit.
PROSITEPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCRTB_PANAN
AccessionPrimary (citable) accession number: P21683
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 16, 2003
Last modified: October 16, 2013
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways