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P21683

- CRTB_PANAN

UniProt

P21683 - CRTB_PANAN

Protein

All-trans-phytoene synthase/15-cis-phytoene synthase

Gene

crtB

Organism
Pantoea ananas (Erwinia uredovora)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 2 (16 May 2003)
      Previous versions | rss
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    Functioni

    Involved in the biosynthesis of carotenoids. Catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to yield both 15-cis and all-trans phytoene isomers.1 Publication

    Catalytic activityi

    2 geranylgeranyl diphosphate = all-trans-phytoene + 2 diphosphate.1 Publication
    2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.1 Publication

    Cofactori

    ATP. ATP is required for the transferase activity but it does not seem to be hydrolyzed during the reaction.1 Publication
    Manganese or magnesium.1 Publication

    Enzyme regulationi

    Inhibited by phosphate ions and squalestatin.1 Publication

    Kineticsi

    1. KM=41 µM for GGPP (at pH 8 and 37 degrees Celsius)1 Publication

    Pathwayi

    GO - Molecular functioni

    1. geranylgeranyl-diphosphate geranylgeranyltransferase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carotenoid biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Carotenoid biosynthesis

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00799; UER00773.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    All-trans-phytoene synthase/15-cis-phytoene synthase (EC:2.5.1.32, EC:2.5.1.99)
    Short name:
    PSase
    Gene namesi
    Name:crtB
    OrganismiPantoea ananas (Erwinia uredovora)
    Taxonomic identifieri553 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePantoea

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309All-trans-phytoene synthase/15-cis-phytoene synthasePRO_0000067429Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP21683.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the phytoene/squalene synthase family.Curated

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    InterProiIPR002060. Squ/phyt_synthse.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF00494. SQS_PSY. 1 hit.
    [Graphical view]
    SUPFAMiSSF48576. SSF48576. 1 hit.
    PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21683-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNPSLLNHA VETMAVGSKS FATASKLFDA KTRRSVLMLY AWCRHCDDVI    50
    DDQTLGFQAR QPALQTPEQR LMQLEMKTRQ AYAGSQMHEP AFAAFQEVAM 100
    AHDIAPAYAF DHLEGFAMDV REAQYSQLDD TLRYCYHVAG VVGLMMAQIM 150
    GVRDNATLDR ACDLGLAFQL TNIARDIVDD AHAGRCYLPA SWLEHEGLNK 200
    ENYAAPENRQ ALSRIARRLV QEAEPYYLSA TAGLAGLPLR SAWAIATAKQ 250
    VYRKIGVKVE QAGQQAWDQR QSTTTPEKLT LLLAASGQAL TSRMRAHPPR 300
    PAHLWQRPL 309
    Length:309
    Mass (Da):34,472
    Last modified:May 16, 2003 - v2
    Checksum:i9AA381A7376BBFC9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90087 Genomic DNA. Translation: BAA14128.2.
    PIRiE37802.
    RefSeqiWP_013027995.1. NZ_CAEJ01000196.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90087 Genomic DNA. Translation: BAA14128.2 .
    PIRi E37802.
    RefSeqi WP_013027995.1. NZ_CAEJ01000196.1.

    3D structure databases

    ProteinModelPortali P21683.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00799 ; UER00773 .

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    InterProi IPR002060. Squ/phyt_synthse.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF00494. SQS_PSY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48576. SSF48576. 1 hit.
    PROSITEi PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli."
      Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., Nakamura K., Harashima K.
      J. Bacteriol. 172:6704-6712(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 20D3.
    2. Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., Nakamura K., Harashima K.
      Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO N-TERMINUS.
    3. "Expression of an active phytoene synthase from Erwinia uredovora and biochemical properties of the enzyme."
      Neudert U., Martinez-Ferez I.M., Fraser P.D., Sandmann G.
      Biochim. Biophys. Acta 1392:51-58(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, COFACTOR.

    Entry informationi

    Entry nameiCRTB_PANAN
    AccessioniPrimary (citable) accession number: P21683
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: May 16, 2003
    Last modified: October 1, 2014
    This is version 65 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3