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Protein

15-cis-phytoene synthase

Gene

crtB

Organism
Pantoea ananas (Erwinia uredovora)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of carotenoids. Catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to yield the 15-cis-phytoene isomer.1 Publication

Caution

The enzyme produces 15-cis-phytoene. The conversion to all-trans-phytoene is due to photoisomerisation.By similarity

Catalytic activityi

2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.1 Publication

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Inhibited by phosphate ions and squalestatin.1 Publication

Kineticsi

  1. KM=41 µM for GGPP (at pH 8 and 37 degrees Celsius)1 Publication

    Pathwayi: phytoene biosynthesis

    This protein is involved in the pathway phytoene biosynthesis, which is part of Carotenoid biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway phytoene biosynthesis and in Carotenoid biosynthesis.

    GO - Molecular functioni

    GO - Biological processi

    • carotenoid biosynthetic process Source: UniProtKB
    • ergosterol biosynthetic process Source: InterPro

    Keywordsi

    Molecular functionTransferase
    Biological processCarotenoid biosynthesis
    LigandMagnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00799

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    15-cis-phytoene synthaseCurated (EC:2.5.1.321 Publication)
    Short name:
    PSase
    Gene namesi
    Name:crtB
    OrganismiPantoea ananas (Erwinia uredovora)
    Taxonomic identifieri553 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaePantoea

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000674291 – 30915-cis-phytoene synthaseAdd BLAST309

    Structurei

    3D structure databases

    ProteinModelPortaliP21683
    SMRiP21683
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the phytoene/squalene synthase family.Curated

    Phylogenomic databases

    eggNOGiENOG4105FE1 Bacteria
    COG1562 LUCA

    Family and domain databases

    CDDicd00683 Trans_IPPS_HH, 1 hit
    Gene3Di1.10.600.10, 1 hit
    InterProiView protein in InterPro
    IPR008949 Isoprenoid_synthase_dom_sf
    IPR002060 Squ/phyt_synthse
    IPR019845 Squalene/phytoene_synthase_CS
    IPR033904 Trans_IPPS_HH
    PfamiView protein in Pfam
    PF00494 SQS_PSY, 1 hit
    SUPFAMiSSF48576 SSF48576, 1 hit
    PROSITEiView protein in PROSITE
    PS01044 SQUALEN_PHYTOEN_SYN_1, 1 hit
    PS01045 SQUALEN_PHYTOEN_SYN_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P21683-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNNPSLLNHA VETMAVGSKS FATASKLFDA KTRRSVLMLY AWCRHCDDVI
    60 70 80 90 100
    DDQTLGFQAR QPALQTPEQR LMQLEMKTRQ AYAGSQMHEP AFAAFQEVAM
    110 120 130 140 150
    AHDIAPAYAF DHLEGFAMDV REAQYSQLDD TLRYCYHVAG VVGLMMAQIM
    160 170 180 190 200
    GVRDNATLDR ACDLGLAFQL TNIARDIVDD AHAGRCYLPA SWLEHEGLNK
    210 220 230 240 250
    ENYAAPENRQ ALSRIARRLV QEAEPYYLSA TAGLAGLPLR SAWAIATAKQ
    260 270 280 290 300
    VYRKIGVKVE QAGQQAWDQR QSTTTPEKLT LLLAASGQAL TSRMRAHPPR

    PAHLWQRPL
    Length:309
    Mass (Da):34,472
    Last modified:May 16, 2003 - v2
    Checksum:i9AA381A7376BBFC9
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D90087 Genomic DNA Translation: BAA14128.2
    PIRiE37802
    RefSeqiWP_013027995.1, NZ_LFLX01000003.1

    Genome annotation databases

    GeneIDi31513106

    Similar proteinsi

    Entry informationi

    Entry nameiCRTB_PANAN
    AccessioniPrimary (citable) accession number: P21683
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: May 16, 2003
    Last modified: April 25, 2018
    This is version 82 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health