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P21683

- CRTB_PANAN

UniProt

P21683 - CRTB_PANAN

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Protein

All-trans-phytoene synthase/15-cis-phytoene synthase

Gene
crtB
Organism
Pantoea ananas (Erwinia uredovora)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of carotenoids. Catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to yield both 15-cis and all-trans phytoene isomers.1 Publication

Catalytic activityi

2 geranylgeranyl diphosphate = all-trans-phytoene + 2 diphosphate.1 Publication
2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.1 Publication

Cofactori

ATP. ATP is required for the transferase activity but it does not seem to be hydrolyzed during the reaction.1 Publication
Manganese or magnesium.1 Publication

Enzyme regulationi

Inhibited by phosphate ions and squalestatin.1 Publication

Kineticsi

  1. KM=41 µM for GGPP (at pH 8 and 37 degrees Celsius)1 Publication

Pathwayi

GO - Molecular functioni

  1. geranylgeranyl-diphosphate geranylgeranyltransferase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carotenoid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carotenoid biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00799; UER00773.

Names & Taxonomyi

Protein namesi
Recommended name:
All-trans-phytoene synthase/15-cis-phytoene synthase (EC:2.5.1.32, EC:2.5.1.99)
Short name:
PSase
Gene namesi
Name:crtB
OrganismiPantoea ananas (Erwinia uredovora)
Taxonomic identifieri553 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePantoea

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309All-trans-phytoene synthase/15-cis-phytoene synthasePRO_0000067429Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP21683.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21683-1 [UniParc]FASTAAdd to Basket

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MNNPSLLNHA VETMAVGSKS FATASKLFDA KTRRSVLMLY AWCRHCDDVI    50
DDQTLGFQAR QPALQTPEQR LMQLEMKTRQ AYAGSQMHEP AFAAFQEVAM 100
AHDIAPAYAF DHLEGFAMDV REAQYSQLDD TLRYCYHVAG VVGLMMAQIM 150
GVRDNATLDR ACDLGLAFQL TNIARDIVDD AHAGRCYLPA SWLEHEGLNK 200
ENYAAPENRQ ALSRIARRLV QEAEPYYLSA TAGLAGLPLR SAWAIATAKQ 250
VYRKIGVKVE QAGQQAWDQR QSTTTPEKLT LLLAASGQAL TSRMRAHPPR 300
PAHLWQRPL 309
Length:309
Mass (Da):34,472
Last modified:May 16, 2003 - v2
Checksum:i9AA381A7376BBFC9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D90087 Genomic DNA. Translation: BAA14128.2.
PIRiE37802.
RefSeqiWP_013027995.1. NZ_CAEJ01000196.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D90087 Genomic DNA. Translation: BAA14128.2 .
PIRi E37802.
RefSeqi WP_013027995.1. NZ_CAEJ01000196.1.

3D structure databases

ProteinModelPortali P21683.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00799 ; UER00773 .

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
InterProi IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF00494. SQS_PSY. 1 hit.
[Graphical view ]
SUPFAMi SSF48576. SSF48576. 1 hit.
PROSITEi PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli."
    Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., Nakamura K., Harashima K.
    J. Bacteriol. 172:6704-6712(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 20D3.
  2. Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., Nakamura K., Harashima K.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO N-TERMINUS.
  3. "Expression of an active phytoene synthase from Erwinia uredovora and biochemical properties of the enzyme."
    Neudert U., Martinez-Ferez I.M., Fraser P.D., Sandmann G.
    Biochim. Biophys. Acta 1392:51-58(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, COFACTOR.

Entry informationi

Entry nameiCRTB_PANAN
AccessioniPrimary (citable) accession number: P21683
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 16, 2003
Last modified: September 3, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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