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Protein

Transcription factor E2-alpha

Gene

Tcf3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer (By similarity). Binds to the consensus sequence CAC/GCTGT/C present, in the chymotrypsin, insulin, AP-4, and several other gene enhancer motifs.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2-alpha
Alternative name(s):
Immunoglobulin enhancer-binding factor E12/E47
Pancreas specific transcription factor 1c
Transcription factor 3
Short name:
TCF-3
Transcription regulator Pan
Gene namesi
Name:Tcf3
Synonyms:Pan, Ptf1c, Tcfe2a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620914. Tcf3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
  • protein complex Source: UniProtKB
  • transcription factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 649649Transcription factor E2-alphaPRO_0000127469Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei135 – 1351PhosphoserineBy similarity
Modified residuei140 – 1401PhosphoserineBy similarity
Modified residuei351 – 3511PhosphothreonineBy similarity
Modified residuei355 – 3551PhosphoserineBy similarity
Cross-linki494 – 494Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Isoform E47 (identifier: P21677-2)
Modified residuei526 – 5261PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylated following NGF stimulation.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP21677.

PTM databases

iPTMnetiP21677.

Interactioni

Subunit structurei

Homodimer. Heterodimer. Forms a heterodimer with MYOG; heterodimerization enhances MYOG DNA-binding and transcriptional activities. Forms a heterodimer with ASH1 and TWIST2. Forms a heterodimer with NEUROD1; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E-box element. Isoform E12 interacts with RALGAPA1 and FIGLA. Interacts with EP300, NEUROD2, PTF1A and TGFB1I1. Component of a nuclear TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3. Efficient DNA binding requires dimerization with another bHLH protein (By similarity). Interacts with UBE2I. Interacts with BHLHA9. Forms a heterodimer with ATOH8; repress transcription of TCF3 and TCF3/NEUROG3 dimer-induced transactivation of E box-dependent promoters (By similarity).By similarity1 Publication

GO - Molecular functioni

  • bHLH transcription factor binding Source: UniProtKB
  • PDZ domain binding Source: RGD
  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • transcription factor binding Source: RGD

Protein-protein interaction databases

BioGridi251063. 4 interactions.
STRINGi10116.ENSRNOP00000023473.

Structurei

3D structure databases

ProteinModelPortaliP21677.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini544 – 59754bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni385 – 42036Leucine-zipperAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi171 – 1777Nuclear localization signalSequence analysis

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3910. Eukaryota.
ENOG410XYUA. LUCA.
HOGENOMiHOG000234180.
HOVERGENiHBG003854.
InParanoidiP21677.
KOiK09063.
PhylomeDBiP21677.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform E12 (identifier: P21677-1) [UniParc]FASTAAdd to basket

Also known as: PAN-2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMNQSQRMAP VGSDKELSDL LDFSMMFPLP VANGKGRPAS LAGTQFAGSG
60 70 80 90 100
LEDRPSSESW GNSEQNSSSF DPSRAYSEGA HFSDSHSSLP PSTFLGAGLG
110 120 130 140 150
GKGSERNAYA TFGRDTSVGT LSQAGFLPGE LGLSSPGPLS PSGVKSSSQY
160 170 180 190 200
YTSFPSNPRR RAADGGLDTQ PKKVRKVPPG LPSSVYPSSS GDNYSRDATA
210 220 230 240 250
YPSAKTPSSA YPSPFYVADG SLHPSAELWS PPGQVGFGPM LGDGSAPLPL
260 270 280 290 300
APGSSSVSSG AFGGLQQQDR MGYQLHGSEV NGTLPAVSSF SAAPGTYSGT
310 320 330 340 350
SGHTPPVSGA DSLLGTRGTT ASSSGDALGK ALASIYSPDH SSNNFSPSPS
360 370 380 390 400
TPVGSPQGLP GTSQWPRAGA PSALSPNYDA GLHGLSKMED RLDEAIHVLR
410 420 430 440 450
SHAVGTASEL HGLLPGHSTL TTSFAGPMSL GGRHAGLVSG SHPEDGLTSG
460 470 480 490 500
ASLLHNHASL PSQPSSLPDL SQRPPDSFSG LGRAGVTAGA SEIKREEKED
510 520 530 540 550
EEVTSVADAE EDKKDLKVPR TRTSPDEDED DLLPPEQKAE REKERRVANN
560 570 580 590 600
ARERLRVRDI NEAFKELGRM CQLHLSTEKP QTKLLILHQA VAVILSLEQQ
610 620 630 640
VRERNLNPKA ACLKRREEEK VSGVVGDPQL ALSAAHPGLG EAHNPAGHL
Length:649
Mass (Da):67,655
Last modified:May 1, 1991 - v1
Checksum:i882F19EDB47D14EA
GO
Isoform E47 (identifier: P21677-2) [UniParc]FASTAAdd to basket

Also known as: PAN-1

The sequence of this isoform differs from the canonical sequence as follows:
     525-596: PDEDEDDLLP...LHQAVAVILS → STDEVLSLEE...LQQAVQVILG

Show »
Length:646
Mass (Da):67,357
Checksum:i140943F09E9A950C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti167 – 1671L → LA in CAA44199 (PubMed:2200736).Curated
Sequence conflicti427 – 4271P → A in AAB21103 (PubMed:1766666).Curated
Sequence conflicti508 – 5081D → DH in AAB21103 (PubMed:1766666).Curated
Sequence conflicti576 – 5772ST → NS in AAB21103 (PubMed:1766666).Curated
Sequence conflicti637 – 6371P → T in AAB21103 (PubMed:1766666).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei525 – 59672PDEDE…AVILS → STDEVLSLEEKDLRDRERRM ANNARERVRVRDINEAFREL GRMCQLHLKSDKAQTKLLIL QQAVQVILG in isoform E47. 1 PublicationVSP_002157Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54549 mRNA. Translation: CAA38421.1.
X62323 mRNA. Translation: CAA44199.1.
AJ973227 mRNA. Translation: CAJ00426.1.
S77532 mRNA. Translation: AAB21103.1.
PIRiA35816.
B35816.
I78853.
RefSeqiNP_001030314.1. NM_001035237.1.
NP_598208.2. NM_133524.2.
UniGeneiRn.10290.

Genome annotation databases

GeneIDi171046.
KEGGirno:171046.
UCSCiRGD:620914. rat. [P21677-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54549 mRNA. Translation: CAA38421.1.
X62323 mRNA. Translation: CAA44199.1.
AJ973227 mRNA. Translation: CAJ00426.1.
S77532 mRNA. Translation: AAB21103.1.
PIRiA35816.
B35816.
I78853.
RefSeqiNP_001030314.1. NM_001035237.1.
NP_598208.2. NM_133524.2.
UniGeneiRn.10290.

3D structure databases

ProteinModelPortaliP21677.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251063. 4 interactions.
STRINGi10116.ENSRNOP00000023473.

PTM databases

iPTMnetiP21677.

Proteomic databases

PaxDbiP21677.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi171046.
KEGGirno:171046.
UCSCiRGD:620914. rat. [P21677-1]

Organism-specific databases

CTDi6929.
RGDi620914. Tcf3.

Phylogenomic databases

eggNOGiKOG3910. Eukaryota.
ENOG410XYUA. LUCA.
HOGENOMiHOG000234180.
HOVERGENiHBG003854.
InParanoidiP21677.
KOiK09063.
PhylomeDBiP21677.

Miscellaneous databases

NextBioi621569.
PROiP21677.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Pan: a transcriptional regulator that binds chymotrypsin, insulin, and AP-4 enhancer motifs."
    Nelson C., Shen L.-P., Meister A., Fodor E., Rutter W.J.
    Genes Dev. 4:1035-1043(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS E12 AND E47).
  2. Wellauer P.K.
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM E12).
    Strain: Sprague-Dawley.
  3. "The helix-loop-helix protein rE12 and the C/EBP-related factor rNFIL-6 bind to neighboring sites within the c-fos serum response element."
    Metz R., Ziff E.
    Oncogene 6:2165-2178(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 427-649 (ISOFORM E12).
  4. "Degradation of E2A proteins through a ubiquitin-conjugating enzyme, UbcE2A."
    Kho C.-J., Huggins G.S., Endege W.O., Hsieh C.-M., Lee M.-E., Haber E.
    J. Biol. Chem. 272:3845-3851(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UBE2I.
    Strain: CD Charles River.
    Tissue: Aorta.
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTFE2_RAT
AccessioniPrimary (citable) accession number: P21677
Secondary accession number(s): P21676, Q08440, Q4VY47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: May 11, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.