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Protein

Transcription factor E2-alpha

Gene

Tcf3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer (By similarity). Binds to the consensus sequence CAC/GCTGT/C present, in the chymotrypsin, insulin, AP-4, and several other gene enhancer motifs (PubMed:2200736).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2-alpha
Alternative name(s):
Immunoglobulin enhancer-binding factor E12/E47
Pancreas specific transcription factor 1c
Transcription factor 3
Short name:
TCF-3
Transcription regulator Pan
Gene namesi
Name:Tcf3
Synonyms:Pan, Ptf1c, Tcfe2a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620914. Tcf3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001274691 – 649Transcription factor E2-alphaAdd BLAST649

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei135PhosphoserineBy similarity1
Modified residuei140PhosphoserineBy similarity1
Modified residuei351PhosphothreonineBy similarity1
Modified residuei355PhosphoserineBy similarity1
Modified residuei367Omega-N-methylarginineBy similarity1
Modified residuei375PhosphoserineBy similarity1
Cross-linki494Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei524PhosphoserineBy similarity1
Cross-linki620Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Isoform E47 (identifier: P21677-2)
Modified residuei526PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated following NGF stimulation.

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP21677.

PTM databases

iPTMnetiP21677.
PhosphoSitePlusiP21677.

Interactioni

Subunit structurei

Homodimer. Heterodimer. Forms a heterodimer with MYOG; heterodimerization enhances MYOG DNA-binding and transcriptional activities. Forms a heterodimer with ASH1 and TWIST2. Forms a heterodimer with NEUROD1; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E-box element. Isoform E12 interacts with RALGAPA1 and FIGLA. Interacts with EP300, NEUROD2, PTF1A and TGFB1I1. Component of a nuclear TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3. Efficient DNA binding requires dimerization with another bHLH protein (By similarity). Interacts with UBE2I. Interacts with BHLHA9. Forms a heterodimer with ATOH8; repress transcription of TCF3 and TCF3/NEUROG3 dimer-induced transactivation of E box-dependent promoters (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi251063. 4 interactors.
CORUMiP21677.
STRINGi10116.ENSRNOP00000023473.

Structurei

3D structure databases

ProteinModelPortaliP21677.
SMRiP21677.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini544 – 597bHLHAdd BLAST54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni385 – 420Leucine-zipperAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi171 – 177Nuclear localization signalSequence analysis7

Phylogenomic databases

eggNOGiKOG3910. Eukaryota.
ENOG410XYUA. LUCA.
HOGENOMiHOG000234180.
HOVERGENiHBG003854.
InParanoidiP21677.
KOiK09063.
PhylomeDBiP21677.

Family and domain databases

CDDicd00083. HLH. 1 hit.
Gene3Di4.10.280.10. 1 hit.
InterProiView protein in InterPro
IPR011598. bHLH_dom.
IPR036638. HLH_DNA-bd_sf.
PfamiView protein in Pfam
PF00010. HLH. 1 hit.
SMARTiView protein in SMART
SM00353. HLH. 1 hit.
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiView protein in PROSITE
PS50888. BHLH. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform E12 (identifier: P21677-1) [UniParc]FASTAAdd to basket
Also known as: PAN-2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMNQSQRMAP VGSDKELSDL LDFSMMFPLP VANGKGRPAS LAGTQFAGSG
60 70 80 90 100
LEDRPSSESW GNSEQNSSSF DPSRAYSEGA HFSDSHSSLP PSTFLGAGLG
110 120 130 140 150
GKGSERNAYA TFGRDTSVGT LSQAGFLPGE LGLSSPGPLS PSGVKSSSQY
160 170 180 190 200
YTSFPSNPRR RAADGGLDTQ PKKVRKVPPG LPSSVYPSSS GDNYSRDATA
210 220 230 240 250
YPSAKTPSSA YPSPFYVADG SLHPSAELWS PPGQVGFGPM LGDGSAPLPL
260 270 280 290 300
APGSSSVSSG AFGGLQQQDR MGYQLHGSEV NGTLPAVSSF SAAPGTYSGT
310 320 330 340 350
SGHTPPVSGA DSLLGTRGTT ASSSGDALGK ALASIYSPDH SSNNFSPSPS
360 370 380 390 400
TPVGSPQGLP GTSQWPRAGA PSALSPNYDA GLHGLSKMED RLDEAIHVLR
410 420 430 440 450
SHAVGTASEL HGLLPGHSTL TTSFAGPMSL GGRHAGLVSG SHPEDGLTSG
460 470 480 490 500
ASLLHNHASL PSQPSSLPDL SQRPPDSFSG LGRAGVTAGA SEIKREEKED
510 520 530 540 550
EEVTSVADAE EDKKDLKVPR TRTSPDEDED DLLPPEQKAE REKERRVANN
560 570 580 590 600
ARERLRVRDI NEAFKELGRM CQLHLSTEKP QTKLLILHQA VAVILSLEQQ
610 620 630 640
VRERNLNPKA ACLKRREEEK VSGVVGDPQL ALSAAHPGLG EAHNPAGHL
Length:649
Mass (Da):67,655
Last modified:May 1, 1991 - v1
Checksum:i882F19EDB47D14EA
GO
Isoform E47 (identifier: P21677-2) [UniParc]FASTAAdd to basket
Also known as: PAN-1

The sequence of this isoform differs from the canonical sequence as follows:
     525-596: PDEDEDDLLP...LHQAVAVILS → STDEVLSLEE...LQQAVQVILG

Show »
Length:646
Mass (Da):67,357
Checksum:i140943F09E9A950C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167L → LA in CAA44199 (PubMed:2200736).Curated1
Sequence conflicti427P → A in AAB21103 (PubMed:1766666).Curated1
Sequence conflicti508D → DH in AAB21103 (PubMed:1766666).Curated1
Sequence conflicti576 – 577ST → NS in AAB21103 (PubMed:1766666).Curated2
Sequence conflicti637P → T in AAB21103 (PubMed:1766666).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002157525 – 596PDEDE…AVILS → STDEVLSLEEKDLRDRERRM ANNARERVRVRDINEAFREL GRMCQLHLKSDKAQTKLLIL QQAVQVILG in isoform E47. 1 PublicationAdd BLAST72

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54549 mRNA. Translation: CAA38421.1.
X62323 mRNA. Translation: CAA44199.1.
AJ973227 mRNA. Translation: CAJ00426.1.
S77532 mRNA. Translation: AAB21103.1.
PIRiA35816.
B35816.
I78853.
RefSeqiNP_001030314.1. NM_001035237.1.
NP_598208.2. NM_133524.2.
UniGeneiRn.10290.

Genome annotation databases

GeneIDi171046.
KEGGirno:171046.
UCSCiRGD:620914. rat. [P21677-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTFE2_RAT
AccessioniPrimary (citable) accession number: P21677
Secondary accession number(s): P21676, Q08440, Q4VY47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 22, 2017
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome