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P21674

- FST_RAT

UniProt

P21674 - FST_RAT

Protein

Follistatin

Gene

Fst

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 May 1991)
      Previous versions | rss
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    Functioni

    Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).

    GO - Molecular functioni

    1. activin binding Source: RGD
    2. heparan sulfate proteoglycan binding Source: RGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. BMP signaling pathway Source: Ensembl
    2. female gonad development Source: Ensembl
    3. gamete generation Source: Ensembl
    4. hair follicle morphogenesis Source: Ensembl
    5. hematopoietic progenitor cell differentiation Source: Ensembl
    6. keratinocyte proliferation Source: Ensembl
    7. negative regulation of activin receptor signaling pathway Source: Ensembl
    8. negative regulation of cell differentiation Source: Ensembl
    9. negative regulation of follicle-stimulating hormone secretion Source: RGD
    10. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    11. odontogenesis of dentin-containing tooth Source: Ensembl
    12. pattern specification process Source: Ensembl
    13. positive regulation of hair follicle development Source: Ensembl

    Enzyme and pathway databases

    ReactomeiREACT_198273. Antagonism of Activin by Follistatin.

    Protein family/group databases

    MEROPSiI01.966.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Follistatin
    Short name:
    FS
    Alternative name(s):
    Activin-binding protein
    Gene namesi
    Name:Fst
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi2633. Fst.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. extracellular region Source: UniProtKB-SubCell
    3. nucleus Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Chaini30 – 344315FollistatinPRO_0000010106Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi32 ↔ 55PROSITE-ProRule annotation
    Disulfide bondi42 ↔ 88PROSITE-ProRule annotation
    Disulfide bondi56 ↔ 91PROSITE-ProRule annotation
    Disulfide bondi95 ↔ 106
    Disulfide bondi100 ↔ 116
    Disulfide bondi118 ↔ 150
    Disulfide bondi122 ↔ 143
    Glycosylationi124 – 1241N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi132 ↔ 164
    Disulfide bondi168 ↔ 179
    Disulfide bondi173 ↔ 189
    Disulfide bondi192 ↔ 225
    Disulfide bondi196 ↔ 218
    Disulfide bondi207 ↔ 239
    Disulfide bondi270 ↔ 302PROSITE-ProRule annotation
    Disulfide bondi274 ↔ 295PROSITE-ProRule annotation
    Disulfide bondi284 ↔ 316PROSITE-ProRule annotation
    Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Gene expression databases

    GenevestigatoriP21674.

    Interactioni

    Subunit structurei

    Monomer.Curated

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    INHBAP084762EBI-5746973,EBI-8077140From a different organism.

    Protein-protein interaction databases

    IntActiP21674. 2 interactions.
    MINTiMINT-1787002.

    Structurei

    Secondary structure

    1
    344
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi104 – 1085
    Beta strandi114 – 1185
    Helixi122 – 1243
    Beta strandi131 – 1333
    Beta strandi138 – 1414
    Helixi142 – 15110
    Beta strandi158 – 1625
    Beta strandi166 – 1683
    Beta strandi178 – 1814
    Beta strandi185 – 1906
    Beta strandi200 – 2023
    Beta strandi206 – 2083
    Beta strandi213 – 2164
    Helixi217 – 22711
    Beta strandi233 – 2386

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1LR7X-ray1.50A93-165[»]
    1LR8X-ray2.10A93-165[»]
    1LR9X-ray2.50A93-165[»]
    2ARPX-ray2.00F93-241[»]
    ProteinModelPortaliP21674.
    SMRiP21674. Positions 30-317.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP21674.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini30 – 10374TBAdd
    BLAST
    Domaini94 – 11724Follistatin-like 1Add
    BLAST
    Domaini112 – 16655Kazal-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini167 – 19024Follistatin-like 2Add
    BLAST
    Domaini186 – 24156Kazal-like 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini244 – 26825Follistatin-like 3Add
    BLAST
    Domaini264 – 31855Kazal-like 3PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi321 – 33313Asp/Glu-rich (highly acidic)Add
    BLAST

    Sequence similaritiesi

    Contains 3 follistatin-like domains.Curated
    Contains 3 Kazal-like domains.PROSITE-ProRule annotation
    Contains 1 TB (TGF-beta binding) domain.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG267610.
    GeneTreeiENSGT00440000033501.
    HOGENOMiHOG000261649.
    HOVERGENiHBG051666.
    InParanoidiP21674.
    KOiK04661.
    OMAiEAVCASD.
    OrthoDBiEOG7GBFXR.
    PhylomeDBiP21674.
    TreeFamiTF106409.

    Family and domain databases

    InterProiIPR003645. Fol_N.
    IPR015369. Follistatin/Osteonectin_EGF.
    IPR002350. Kazal_dom.
    IPR017878. TB_dom.
    [Graphical view]
    PfamiPF09289. FOLN. 1 hit.
    PF07648. Kazal_2. 3 hits.
    [Graphical view]
    SMARTiSM00274. FOLN. 3 hits.
    SM00280. KAZAL. 3 hits.
    [Graphical view]
    SUPFAMiSSF57581. SSF57581. 1 hit.
    PROSITEiPS51465. KAZAL_2. 3 hits.
    PS51364. TB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P21674-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVCARHQPGG LCLLLLLLCQ FMEDRSAQAG NCWLRQAKNG RCQVLYKTEL    50
    SKEECCSTGR LSTSWTEEDV NDNTLFKWMI FNGGAPNCIP CKETCENVDC 100
    GPGKKCRMNK KNKPRCVCAP DCSNITWKGP VCGLDGKTYR NECALLKARC 150
    KEQPELEVQY QGKCKKTCRD VFCPGSSTCV VDQTNNAYCV TCNRICPEPS 200
    SSEQSLCGND GVTYSSACHL RKATCLLGRS IGLAYEGKCI KAKSCEDIQC 250
    GGGKKCLWDF KVGRGRCSLC DELCPDSKSD EPVCASDNAT YASECAMKEA 300
    ACSSGVLLEV KHSGSCNSIS EETEEEEEEE DQDYSFPISS TLEW 344
    Length:344
    Mass (Da):37,838
    Last modified:May 1, 1991 - v1
    Checksum:i864244CF05436552
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31591
    , M31586, M31587, M31588, M31589, M31590 Genomic DNA. Translation: AAB60704.1.
    S58913 Genomic DNA. Translation: AAP13905.1.
    PIRiI57698.
    RefSeqiNP_036693.1. NM_012561.1.
    UniGeneiRn.162557.

    Genome annotation databases

    EnsembliENSRNOT00000015680; ENSRNOP00000015680; ENSRNOG00000011631.
    GeneIDi24373.
    KEGGirno:24373.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31591
    , M31586 , M31587 , M31588 , M31589 , M31590 Genomic DNA. Translation: AAB60704.1 .
    S58913 Genomic DNA. Translation: AAP13905.1 .
    PIRi I57698.
    RefSeqi NP_036693.1. NM_012561.1.
    UniGenei Rn.162557.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1LR7 X-ray 1.50 A 93-165 [» ]
    1LR8 X-ray 2.10 A 93-165 [» ]
    1LR9 X-ray 2.50 A 93-165 [» ]
    2ARP X-ray 2.00 F 93-241 [» ]
    ProteinModelPortali P21674.
    SMRi P21674. Positions 30-317.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P21674. 2 interactions.
    MINTi MINT-1787002.

    Protein family/group databases

    MEROPSi I01.966.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000015680 ; ENSRNOP00000015680 ; ENSRNOG00000011631 .
    GeneIDi 24373.
    KEGGi rno:24373.

    Organism-specific databases

    CTDi 10468.
    RGDi 2633. Fst.

    Phylogenomic databases

    eggNOGi NOG267610.
    GeneTreei ENSGT00440000033501.
    HOGENOMi HOG000261649.
    HOVERGENi HBG051666.
    InParanoidi P21674.
    KOi K04661.
    OMAi EAVCASD.
    OrthoDBi EOG7GBFXR.
    PhylomeDBi P21674.
    TreeFami TF106409.

    Enzyme and pathway databases

    Reactomei REACT_198273. Antagonism of Activin by Follistatin.

    Miscellaneous databases

    EvolutionaryTracei P21674.
    NextBioi 603119.
    PROi P21674.

    Gene expression databases

    Genevestigatori P21674.

    Family and domain databases

    InterProi IPR003645. Fol_N.
    IPR015369. Follistatin/Osteonectin_EGF.
    IPR002350. Kazal_dom.
    IPR017878. TB_dom.
    [Graphical view ]
    Pfami PF09289. FOLN. 1 hit.
    PF07648. Kazal_2. 3 hits.
    [Graphical view ]
    SMARTi SM00274. FOLN. 3 hits.
    SM00280. KAZAL. 3 hits.
    [Graphical view ]
    SUPFAMi SSF57581. SSF57581. 1 hit.
    PROSITEi PS51465. KAZAL_2. 3 hits.
    PS51364. TB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Follistatin gene expression in the ovary and extragonadal tissues."
      Shimasaki S., Koga M., Buscaglia M.L., Simmons D.M., Bicsak T.A., Ling N.
      Mol. Endocrinol. 3:651-659(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Ovary.
    2. "Structural and functional characterization of the rat follistatin (activin-binding protein) gene promoter."
      Miyanaga K., Shimasaki S.
      Mol. Cell. Endocrinol. 92:99-109(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
    3. "Crystal structures of the heparan sulfate-binding domain of follistatin. Insights into ligand binding."
      Innis C.A., Hyvonen M.
      J. Biol. Chem. 278:39969-39977(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 93-165 ALONE AND IN COMPLEX WITH HEPARIN ANALOGS, DISULFIDE BONDS.
    4. "Structural basis for the inhibition of activin signalling by follistatin."
      Harrington A.E., Morris-Triggs S.A., Ruotolo B.T., Robinson C.V., Ohnuma S., Hyvonen M.
      EMBO J. 25:1035-1045(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 93-241 IN COMPLEX WITH ACTIVIN A, DISULFIDE BONDS.

    Entry informationi

    Entry nameiFST_RAT
    AccessioniPrimary (citable) accession number: P21674
    Secondary accession number(s): Q80XW7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: May 1, 1991
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3