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P21671

- PLCD4_BOVIN

UniProt

P21671 - PLCD4_BOVIN

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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4

Gene

PLCD4

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca2+ from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca2+ mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation (By similarity).By similarity

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Binds 3 calcium ions per subunit. Two of the calcium ions are bound to the C2 domain (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei305 – 3051PROSITE-ProRule annotation
Metal bindingi306 – 3061Calcium 1; catalyticBy similarity
Metal bindingi335 – 3351Calcium 1; catalyticBy similarity
Metal bindingi337 – 3371Calcium 1; catalyticBy similarity
Active sitei350 – 3501PROSITE-ProRule annotation
Metal bindingi384 – 3841Calcium 1; catalyticBy similarity
Binding sitei433 – 4331SubstrateBy similarity
Binding sitei435 – 4351SubstrateBy similarity
Binding sitei551 – 5511SubstrateBy similarity
Binding sitei578 – 5781SubstrateBy similarity
Metal bindingi679 – 6791Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi681 – 6811Calcium 2By similarity
Metal bindingi705 – 7051Calcium 2By similarity
Metal bindingi734 – 7341Calcium 3By similarity
Metal bindingi735 – 7351Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi736 – 7361Calcium 3By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi147 – 158121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi183 – 194122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. acrosome reaction Source: InterPro
  2. intracellular signal transduction Source: InterPro
  3. lipid catabolic process Source: UniProtKB-KW
  4. phosphatidylinositol metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 (EC:3.1.4.11)
Alternative name(s):
PLC-85
Phosphoinositide phospholipase C-delta-4
Phospholipase C-delta-2
Short name:
PLC-delta-2
Phospholipase C-delta-4
Short name:
PLC-delta-4
Gene namesi
Name:PLCD4
Synonyms:PLCD2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Membrane By similarity; Peripheral membrane protein By similarity. Nucleus By similarity. Cytoplasm By similarity. Endoplasmic reticulum By similarity
Note: Localizes primarily to intracellular membranes mostly to the endoplasmic reticulum.By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
  3. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7917911-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4PRO_0000088508Add
BLAST

Proteomic databases

PRIDEiP21671.

Interactioni

Subunit structurei

Interacts with GRIP1.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000041545.

Structurei

3D structure databases

ProteinModelPortaliP21671.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 124109PHPROSITE-ProRule annotationAdd
BLAST
Domaini134 – 16936EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini170 – 20536EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini203 – 23735EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini290 – 435146PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini522 – 638117PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini643 – 748106C2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni26 – 5328Substrate bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi760 – 7634PDZ-binding

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi440 – 45516Poly-GluAdd
BLAST

Domaini

The PDZ-binding motif mediates the interaction with GRIP1.By similarity
The C2 domain mediates pre-localization to the membrane prior to Ca2+ import and non-selective Ca2+-mediated targeting to various cellular membranes.By similarity
The PH domain is not a critical determinant of the membrane localization.By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 3 EF-hand domains.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000006871.
HOVERGENiHBG053610.
InParanoidiP21671.
KOiK05857.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR028387. PLC-delta4.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF31. PTHR10336:SF31. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00169. PH. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00054. EFh. 3 hits.
SM00233. PH. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21671-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAYLLQGRLP INQDLLLMQK GTMMRKVRSK SWKKLRFFRL QDDGMTVWHA
60 70 80 90 100
RQAGGRAKPS FSISDVDTVR EDHESELLRN LAEEFPLEQG FTIVFHGRRS
110 120 130 140 150
NLDLVANSVQ EAQTWMQGLQ LLVGFVTNMD QQERLDQWLS DWFQRGDKNQ
160 170 180 190 200
DGRMSFGEVQ RLLHLMNVEM DQEYAFQLFQ TADTSQSGTL EGEEFVEFYK
210 220 230 240 250
SLTQRPEVQE LFEKFSSDGQ KLTLLEFVDF LQEEQKEGER ASDLALELID
260 270 280 290 300
RYEPSESGKL RHVLSMDGFL GYLCSKDGDI FNPTCHPLYQ DMTQPLNHYY
310 320 330 340 350
INSSHNTYLV GDQLCGQSSV EGYIRALKRG CRCVEVDIWD GPSGEPIVYH
360 370 380 390 400
GHTLTSRIPF KDVVAAIGQY AFQTSDYPVI LSLENHCSWE QQEIIVRHLT
410 420 430 440 450
EILGDQLLTT ALDGQPPTQL PSPEDLRGKI LVKGKKLMLE EEEEEPEAEL
460 470 480 490 500
EAEQEARLDL EAQLESEPQD LSPRSEDKKK VVMCPLLCRP LCCQIMAQAP
510 520 530 540 550
ISKPGLLLFP KQKPKAILCP ALSALVVYLK AVTFYSFTHS REHYHFYETS
560 570 580 590 600
SFSETKAKSL IKEAGDEFVQ HNAWQLSRVY PSGLRTDSSN YNPQEFWNAG
610 620 630 640 650
CQMVAMNMQT AGLEMDLCDG LFRQNAGCGY VLKPDFLRDA QSSFHPERPI
660 670 680 690 700
SPFKAQTLII QVISGQQLPK VDNTKEQSIV DPLVRVEIFG VRPDTTRQET
710 720 730 740 750
SYVENNGFNP YWGQTLCFRI LVPELALLRF VVKDYDWKSR NDFIGQYTLP
760 770 780 790
WSCMQQGYRH IHLLSKDGLS LHPASIFVHI CTQEVSEEAE S
Length:791
Mass (Da):90,581
Last modified:October 2, 2007 - v2
Checksum:i33E4C4278F280CD2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC112449 mRNA. Translation: AAI12450.1.
RefSeqiNP_001039954.1. NM_001046489.2.
UniGeneiBt.45064.

Genome annotation databases

GeneIDi540771.
KEGGibta:540771.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC112449 mRNA. Translation: AAI12450.1 .
RefSeqi NP_001039954.1. NM_001046489.2.
UniGenei Bt.45064.

3D structure databases

ProteinModelPortali P21671.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000041545.

Proteomic databases

PRIDEi P21671.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 540771.
KEGGi bta:540771.

Organism-specific databases

CTDi 84812.

Phylogenomic databases

eggNOGi NOG149692.
HOGENOMi HOG000006871.
HOVERGENi HBG053610.
InParanoidi P21671.
KOi K05857.

Miscellaneous databases

NextBioi 20878827.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR028387. PLC-delta4.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
PTHR10336:SF31. PTHR10336:SF31. 1 hit.
Pfami PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00169. PH. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view ]
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00054. EFh. 3 hits.
SM00233. PH. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.
  2. "A novel inositol-phospholipid-specific phospholipase C. Rapid purification and characterization."
    Meldrum E., Katan M., Parker P.
    Eur. J. Biochem. 182:673-677(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 560-584 AND 685-696.
  3. "Phospholipase C delta-type consists of three isozymes: bovine PLCdelta2 is a homologue of human/mouse PLCdelta4."
    Irino Y., Cho H., Nakamura Y., Nakahara M., Furutani M., Suh P.-G., Takenawa T., Fukami K.
    Biochem. Biophys. Res. Commun. 320:537-543(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.

Entry informationi

Entry nameiPLCD4_BOVIN
AccessioniPrimary (citable) accession number: P21671
Secondary accession number(s): Q2KIZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: October 2, 2007
Last modified: October 29, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was initially (PubMed:2753038) named PLCD2 and was thought to be a new member of the PLC-delta family. It was later shown that it corresponds to PLCD4 (PubMed:15219862).2 Publications

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3