P21670 (PSA4_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proteasome subunit alpha type-4
EC=3.4.25.1

Alternative name(s):
Macropain subunit C9
Multicatalytic endopeptidase complex subunit C9
Proteasome component C9
Proteasome subunit L

Gene names

Name:

Psma4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.
Subcellular location	Cytoplasm. Nucleus.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex, alpha-subunit complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Proteasome subunit alpha type-4		PRO_0000124106
Amino acid modifications
	Modified residue	13	1	Phosphoserine By similarity
	Modified residue	75	1	Phosphoserine By similarity
	Modified residue	127	1	N6-acetyllysine By similarity
	Modified residue	176	1	N6-acetyllysine By similarity
	Modified residue	238	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21670 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: BB6742398E91E13B	FASTA	261	29,498
	10 20 30 40 50 60 MSRRYDSRTT IFSPEGRLYQ VEYAMEAIGH AGTCLGILAN DGVLLAAERR NIHKLLDEVF 70 80 90 100 110 120 FSEKIYKLNE DMACSVAGIT SDANVLTNEL RLIAQRYLLQ YQEPIPCEQL VTALCDIKQA 130 140 150 160 170 180 YTQFGGKRPF GVSLLYIGWD KHYGFQLYQS DPSGNYGGWK ATCIGNNSAA AVSMLKQDYK 190 200 210 220 230 240 EGEMTLKSAL ALAVKVLNKT MDVSKLSAEK VEIATLTREN GKTVIRVLKQ KEVEQLIKKH 250 260 EEEEAKAERE KKEKEQREKD K

References

[1]	"cDNA cloning and sequencing of component C9 of proteasomes from rat hepatoma cells." Kumatori A., Tanaka K., Tamura T., Fujiwara T., Ichihara A., Tokunaga F., Onikura A., Iwanaga S. FEBS Lett. 264:279-282(1990) [PubMed: 2358075] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[2]	"Molecular cloning of cDNAs for two subunits of rat multicatalytic proteinase. Existence of N-terminal conserved and C-terminal diverged sequences among subunits." Sorimachi H., Tsukahara T., Kawasaki H., Ishiura S., Emori Y., Sugita H., Suzuki K. Eur. J. Biochem. 193:775-781(1990) [PubMed: 2249692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53304 mRNA. Translation: CAA37390.1. X55986 mRNA. Translation: CAA39458.1.
IPI	IPI00231046.
PIR	SNRTC9. S10566.
RefSeq	NP_058977.1. NM_017281.1.
UniGene	Rn.11076.
3D structure databases
ProteinModelPortal	P21670.
SMR	P21670. Positions 2-237.
ModBase	Search...
Protein-protein interaction databases
IntAct	P21670. 2 interactions.
STRING	P21670.
Protein family/group databases
MEROPS	T01.973.
2D gel databases
World-2DPAGE	0004:P21670.
Proteomic databases
PRIDE	P21670.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000018173; ENSRNOP00000018173; ENSRNOG00000013493.
GeneID	29671.
KEGG	rno:29671.
NMPDR	fig|10116.3.peg.28634.
UCSC	NM_017281. rat.
Organism-specific databases
CTD	5685.
RGD	61846. Psma4.
Phylogenomic databases
eggNOG	roNOG06617.
HOVERGEN	HBG003005.
InParanoid	P21670.
OrthoDB	EOG49079S.
PhylomeDB	P21670.
Gene expression databases
ArrayExpress	P21670.
Genevestigator	P21670.
GermOnline	ENSRNOG00000013493. Rattus norvegicus.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_N. IPR016050. Proteasome_bsu_CS. IPR023332. Proteasome_suA-type. IPR001353. Proteasome_sua/b. [Graphical view]
KO	K02728.
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
SMART	SM00948. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A_1. 1 hit. PS51475. PROTEASOME_A_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	609995.

Entry information

Entry name

PSA4_RAT

Accession

Primary (citable) accession number: P21670

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	January 25, 2012
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program