SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P21670

- PSA4_RAT

UniProt

P21670 - PSA4_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Proteasome subunit alpha type-4
Gene
Psma4
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiREACT_194781. Separation of Sister Chromatids.
REACT_196424. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_198391. Asymmetric localization of PCP proteins.
REACT_199194. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_199197. ER-Phagosome pathway.
REACT_199247. Activation of NF-kappaB in B cells.
REACT_199254. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_204983. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_206488. degradation of DVL.
REACT_206997. CDT1 association with the CDC6:ORC:origin complex.
REACT_211117. Orc1 removal from chromatin.
REACT_212486. CDK-mediated phosphorylation and removal of Cdc6.
REACT_220232. Regulation of ornithine decarboxylase (ODC).
REACT_227706. degradation of AXIN.

Protein family/group databases

MEROPSiT01.973.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-4 (EC:3.4.25.1)
Alternative name(s):
Macropain subunit C9
Multicatalytic endopeptidase complex subunit C9
Proteasome component C9
Proteasome subunit L
Gene namesi
Name:Psma4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 8

Organism-specific databases

RGDi61846. Psma4.

Subcellular locationi

Cytoplasm. Nucleus. CytoplasmP-body By similarity
Note: Colocalizes with TRIM5 in the cytoplasmic bodies By similarity.

GO - Cellular componenti

  1. cytoplasmic mRNA processing body Source: UniProtKB
  2. nucleus Source: UniProtKB-SubCell
  3. proteasome core complex Source: UniProtKB
  4. proteasome core complex, alpha-subunit complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Proteasome subunit alpha type-4
PRO_0000124106Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131Phosphoserine By similarity
Modified residuei75 – 751Phosphoserine By similarity
Modified residuei127 – 1271N6-acetyllysine By similarity
Modified residuei176 – 1761N6-acetyllysine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP21670.
PRIDEiP21670.

2D gel databases

World-2DPAGE0004:P21670.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

GenevestigatoriP21670.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.

Protein-protein interaction databases

IntActiP21670. 2 interactions.
STRINGi10116.ENSRNOP00000018173.

Structurei

3D structure databases

ProteinModelPortaliP21670.
SMRiP21670. Positions 2-237.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.

Phylogenomic databases

eggNOGiCOG0638.
GeneTreeiENSGT00550000074827.
HOGENOMiHOG000091085.
HOVERGENiHBG003005.
InParanoidiP21670.
KOiK02728.
OMAiKQEYKDD.
OrthoDBiEOG7F512J.
PhylomeDBiP21670.
TreeFamiTF106209.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR016050. Proteasome_bsu_CS.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21670-1 [UniParc]FASTAAdd to Basket

« Hide

MSRRYDSRTT IFSPEGRLYQ VEYAMEAIGH AGTCLGILAN DGVLLAAERR    50
NIHKLLDEVF FSEKIYKLNE DMACSVAGIT SDANVLTNEL RLIAQRYLLQ 100
YQEPIPCEQL VTALCDIKQA YTQFGGKRPF GVSLLYIGWD KHYGFQLYQS 150
DPSGNYGGWK ATCIGNNSAA AVSMLKQDYK EGEMTLKSAL ALAVKVLNKT 200
MDVSKLSAEK VEIATLTREN GKTVIRVLKQ KEVEQLIKKH EEEEAKAERE 250
KKEKEQREKD K 261
Length:261
Mass (Da):29,498
Last modified:May 1, 1991 - v1
Checksum:iBB6742398E91E13B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53304 mRNA. Translation: CAA37390.1.
X55986 mRNA. Translation: CAA39458.1.
PIRiS10566. SNRTC9.
RefSeqiNP_058977.1. NM_017281.1.
XP_006243128.1. XM_006243066.1.
UniGeneiRn.11076.

Genome annotation databases

EnsembliENSRNOT00000018173; ENSRNOP00000018173; ENSRNOG00000013493.
GeneIDi29671.
KEGGirno:29671.
UCSCiRGD:61846. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53304 mRNA. Translation: CAA37390.1 .
X55986 mRNA. Translation: CAA39458.1 .
PIRi S10566. SNRTC9.
RefSeqi NP_058977.1. NM_017281.1.
XP_006243128.1. XM_006243066.1.
UniGenei Rn.11076.

3D structure databases

ProteinModelPortali P21670.
SMRi P21670. Positions 2-237.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P21670. 2 interactions.
STRINGi 10116.ENSRNOP00000018173.

Protein family/group databases

MEROPSi T01.973.

2D gel databases

World-2DPAGE 0004:P21670.

Proteomic databases

PaxDbi P21670.
PRIDEi P21670.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000018173 ; ENSRNOP00000018173 ; ENSRNOG00000013493 .
GeneIDi 29671.
KEGGi rno:29671.
UCSCi RGD:61846. rat.

Organism-specific databases

CTDi 5685.
RGDi 61846. Psma4.

Phylogenomic databases

eggNOGi COG0638.
GeneTreei ENSGT00550000074827.
HOGENOMi HOG000091085.
HOVERGENi HBG003005.
InParanoidi P21670.
KOi K02728.
OMAi KQEYKDD.
OrthoDBi EOG7F512J.
PhylomeDBi P21670.
TreeFami TF106209.

Enzyme and pathway databases

Reactomei REACT_194781. Separation of Sister Chromatids.
REACT_196424. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_198391. Asymmetric localization of PCP proteins.
REACT_199194. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_199197. ER-Phagosome pathway.
REACT_199247. Activation of NF-kappaB in B cells.
REACT_199254. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_204983. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_206488. degradation of DVL.
REACT_206997. CDT1 association with the CDC6:ORC:origin complex.
REACT_211117. Orc1 removal from chromatin.
REACT_212486. CDK-mediated phosphorylation and removal of Cdc6.
REACT_220232. Regulation of ornithine decarboxylase (ODC).
REACT_227706. degradation of AXIN.

Miscellaneous databases

NextBioi 609995.
PROi P21670.

Gene expression databases

Genevestigatori P21670.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
InterProi IPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR016050. Proteasome_bsu_CS.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view ]
SMARTi SM00948. Proteasome_A_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning and sequencing of component C9 of proteasomes from rat hepatoma cells."
    Kumatori A., Tanaka K., Tamura T., Fujiwara T., Ichihara A., Tokunaga F., Onikura A., Iwanaga S.
    FEBS Lett. 264:279-282(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.
  2. "Molecular cloning of cDNAs for two subunits of rat multicatalytic proteinase. Existence of N-terminal conserved and C-terminal diverged sequences among subunits."
    Sorimachi H., Tsukahara T., Kawasaki H., Ishiura S., Emori Y., Sugita H., Suzuki K.
    Eur. J. Biochem. 193:775-781(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.

Entry informationi

Entry nameiPSA4_RAT
AccessioniPrimary (citable) accession number: P21670
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: September 3, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi