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P21670 (PSA4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit alpha type-4

EC=3.4.25.1
Alternative name(s):
Macropain subunit C9
Multicatalytic endopeptidase complex subunit C9
Proteasome component C9
Proteasome subunit L
Gene names
Name:Psma4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.

Subcellular location

Cytoplasm. Nucleus. CytoplasmP-body By similarity. Note: Colocalizes with TRIM5 in the cytoplasmic bodies By similarity.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the peptidase T1A family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 261261Proteasome subunit alpha type-4
PRO_0000124106

Amino acid modifications

Modified residue131Phosphoserine By similarity
Modified residue751Phosphoserine By similarity
Modified residue1271N6-acetyllysine By similarity
Modified residue1761N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P21670 [UniParc].

Last modified May 1, 1991. Version 1.
Checksum: BB6742398E91E13B

FASTA26129,498
        10         20         30         40         50         60 
MSRRYDSRTT IFSPEGRLYQ VEYAMEAIGH AGTCLGILAN DGVLLAAERR NIHKLLDEVF 

        70         80         90        100        110        120 
FSEKIYKLNE DMACSVAGIT SDANVLTNEL RLIAQRYLLQ YQEPIPCEQL VTALCDIKQA 

       130        140        150        160        170        180 
YTQFGGKRPF GVSLLYIGWD KHYGFQLYQS DPSGNYGGWK ATCIGNNSAA AVSMLKQDYK 

       190        200        210        220        230        240 
EGEMTLKSAL ALAVKVLNKT MDVSKLSAEK VEIATLTREN GKTVIRVLKQ KEVEQLIKKH 

       250        260 
EEEEAKAERE KKEKEQREKD K 

« Hide

References

[1]"cDNA cloning and sequencing of component C9 of proteasomes from rat hepatoma cells."
Kumatori A., Tanaka K., Tamura T., Fujiwara T., Ichihara A., Tokunaga F., Onikura A., Iwanaga S.
FEBS Lett. 264:279-282(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Liver.
[2]"Molecular cloning of cDNAs for two subunits of rat multicatalytic proteinase. Existence of N-terminal conserved and C-terminal diverged sequences among subunits."
Sorimachi H., Tsukahara T., Kawasaki H., Ishiura S., Emori Y., Sugita H., Suzuki K.
Eur. J. Biochem. 193:775-781(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53304 mRNA. Translation: CAA37390.1.
X55986 mRNA. Translation: CAA39458.1.
PIRSNRTC9. S10566.
RefSeqNP_058977.1. NM_017281.1.
XP_006243128.1. XM_006243066.1.
UniGeneRn.11076.

3D structure databases

ProteinModelPortalP21670.
SMRP21670. Positions 2-237.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP21670. 2 interactions.
STRING10116.ENSRNOP00000018173.

Protein family/group databases

MEROPST01.973.

2D gel databases

World-2DPAGE0004:P21670.

Proteomic databases

PaxDbP21670.
PRIDEP21670.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000018173; ENSRNOP00000018173; ENSRNOG00000013493.
GeneID29671.
KEGGrno:29671.
UCSCRGD:61846. rat.

Organism-specific databases

CTD5685.
RGD61846. Psma4.

Phylogenomic databases

eggNOGCOG0638.
GeneTreeENSGT00550000074827.
HOGENOMHOG000091085.
HOVERGENHBG003005.
InParanoidP21670.
KOK02728.
OMAKQEYKDD.
OrthoDBEOG7F512J.
PhylomeDBP21670.
TreeFamTF106209.

Gene expression databases

GenevestigatorP21670.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR016050. Proteasome_bsu_CS.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio609995.
PROP21670.

Entry information

Entry namePSA4_RAT
AccessionPrimary (citable) accession number: P21670
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: June 11, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries