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P21661

- NEC2_MOUSE

UniProt

P21661 - NEC2_MOUSE

Protein

Neuroendocrine convertase 2

Gene

Pcsk2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 1 (01 May 1991)
      Previous versions | rss
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    Functioni

    Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Responsible for the release of glucagon from proglucagon in pancreatic A cells By similarity.By similarity

    Catalytic activityi

    Release of protein hormones and neuropeptides from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei166 – 1661Charge relay systemBy similarity
    Active sitei207 – 2071Charge relay systemBy similarity
    Active sitei383 – 3831Charge relay systemBy similarity

    GO - Molecular functioni

    1. endopeptidase activity Source: BHF-UCL
    2. serine-type endopeptidase activity Source: BHF-UCL

    GO - Biological processi

    1. embryo development Source: Ensembl
    2. enkephalin processing Source: BHF-UCL
    3. insulin processing Source: Ensembl
    4. islet amyloid polypeptide processing Source: Ensembl
    5. nervous system development Source: HGNC
    6. peptide hormone processing Source: BHF-UCL
    7. protein autoprocessing Source: HGNC
    8. protein processing Source: BHF-UCL
    9. proteolysis Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    ReactomeiREACT_206713. NGF processing.
    REACT_207837. Insulin processing.

    Protein family/group databases

    MEROPSiS08.073.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuroendocrine convertase 2 (EC:3.4.21.94)
    Short name:
    NEC 2
    Alternative name(s):
    KEX2-like endoprotease 2
    Prohormone convertase 2
    Proprotein convertase 2
    Short name:
    PC2
    Gene namesi
    Name:Pcsk2
    Synonyms:Nec-2, Nec2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:97512. Pcsk2.

    Subcellular locationi

    Cytoplasmic vesiclesecretory vesicle
    Note: Localized in the secretion granules.

    GO - Cellular componenti

    1. dendrite Source: Ensembl
    2. endoplasmic reticulum lumen Source: Reactome
    3. extracellular space Source: BHF-UCL
    4. membrane Source: Ensembl
    5. perikaryon Source: Ensembl
    6. transport vesicle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasmic vesicle

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Propeptidei25 – 10884Sequence AnalysisPRO_0000027067Add
    BLAST
    Chaini109 – 637529Neuroendocrine convertase 2PRO_0000027068Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi224 ↔ 375By similarity
    Disulfide bondi316 ↔ 346By similarity
    Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi467 ↔ 493By similarity
    Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi523 – 5231N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiP21661.
    PRIDEiP21661.

    Miscellaneous databases

    PMAP-CutDBP21661.

    Expressioni

    Gene expression databases

    BgeeiP21661.
    CleanExiMM_PCSK2.
    GenevestigatoriP21661.

    Interactioni

    Protein-protein interaction databases

    IntActiP21661. 1 interaction.
    MINTiMINT-1508709.

    Structurei

    3D structure databases

    ProteinModelPortaliP21661.
    SMRiP21661. Positions 122-593.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini161 – 452292Peptidase S8Add
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S8 family. Furin subfamily.Curated
    Contains 1 peptidase S8 domain.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4935.
    HOVERGENiHBG008705.
    InParanoidiP21661.
    KOiK01360.
    OMAiDVQHLTV.
    OrthoDBiEOG7BW0JD.
    TreeFamiTF314277.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    3.40.50.200. 1 hit.
    InterProiIPR008979. Galactose-bd-like.
    IPR000209. Peptidase_S8/S53_dom.
    IPR023827. Peptidase_S8_Asp-AS.
    IPR022398. Peptidase_S8_His-AS.
    IPR023828. Peptidase_S8_Ser-AS.
    IPR015500. Peptidase_S8_subtilisin-rel.
    IPR009020. Prot_inh_propept.
    IPR002884. PrprotnconvertsP.
    [Graphical view]
    PANTHERiPTHR10795. PTHR10795. 1 hit.
    PfamiPF01483. P_proprotein. 1 hit.
    PF00082. Peptidase_S8. 1 hit.
    [Graphical view]
    PRINTSiPR00723. SUBTILISIN.
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF52743. SSF52743. 1 hit.
    SSF54897. SSF54897. 1 hit.
    PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
    PS00137. SUBTILASE_HIS. 1 hit.
    PS00138. SUBTILASE_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P21661-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGGCGSQWK AAGFLFCVMV FASAERPVFT NHFLVELHKD GEEEARQVAA    50
    EHGFGVRKLP FAEGLYHFYH NGLAKAKRRR SLHHKRQLER DPRIKMALQQ 100
    EGFDRKKRGY RDINEIDINM NDPLFTKQWY LFNTGQADGT PGLDLNVAEA 150
    WELGYTGKGV TIGIMDDGID YLHPDLAYNY NADASYDFSS NDPYPYPRYT 200
    DDWFNSHGTR CAGEVSAAAS NNICGVGVAY NSKVAGIRML DQPFMTDIIE 250
    ASSISHMPQL IDIYSASWGP TDNGKTVDGP RELTLQAMAD GVNKGRGGKG 300
    SIYVWASGDG GSYDDCNCDG YASSMWTISI NSAINDGRTA LYDESCSSTL 350
    ASTFSNGRKR NPEAGVATTD LYGNCTLRHS GTSAAAPEAA GVFALALEAN 400
    LDLTWRDMQH LTVLTSKRNQ LHDEVHQWRR NGVGLEFNHL FGYGVLDAGA 450
    MVKMAKDWKT VPERFHCVGG SVQNPEKIPP TGKLVLTLKT NACEGKENFV 500
    RYLEHVQAVI TVNATRRGDL NINMTSPMGT KSILLSRRPR DDDSKVGFDK 550
    WPFMTTHTWG EDARGTWTLE LGFVGSAPQK GLLKEWTLML HGTQSAPYID 600
    QVVRDYQSKL AMSKKQELEE ELDEAVERSL QSILRKN 637
    Length:637
    Mass (Da):70,785
    Last modified:May 1, 1991 - v1
    Checksum:iB69F2DCCD00FB0E6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55669 mRNA. Translation: AAA39376.1.
    BC052013 mRNA. Translation: AAH52013.2.
    BC057348 mRNA. Translation: AAH57348.1.
    CCDSiCCDS16810.1.
    PIRiB35571. KXMSC2.
    RefSeqiNP_032818.1. NM_008792.4.
    UniGeneiMm.294493.
    Mm.447466.

    Genome annotation databases

    EnsembliENSMUST00000028905; ENSMUSP00000028905; ENSMUSG00000027419.
    GeneIDi18549.
    KEGGimmu:18549.
    UCSCiuc008mqf.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55669 mRNA. Translation: AAA39376.1 .
    BC052013 mRNA. Translation: AAH52013.2 .
    BC057348 mRNA. Translation: AAH57348.1 .
    CCDSi CCDS16810.1.
    PIRi B35571. KXMSC2.
    RefSeqi NP_032818.1. NM_008792.4.
    UniGenei Mm.294493.
    Mm.447466.

    3D structure databases

    ProteinModelPortali P21661.
    SMRi P21661. Positions 122-593.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P21661. 1 interaction.
    MINTi MINT-1508709.

    Protein family/group databases

    MEROPSi S08.073.

    Proteomic databases

    PaxDbi P21661.
    PRIDEi P21661.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028905 ; ENSMUSP00000028905 ; ENSMUSG00000027419 .
    GeneIDi 18549.
    KEGGi mmu:18549.
    UCSCi uc008mqf.2. mouse.

    Organism-specific databases

    CTDi 5126.
    MGIi MGI:97512. Pcsk2.

    Phylogenomic databases

    eggNOGi COG4935.
    HOVERGENi HBG008705.
    InParanoidi P21661.
    KOi K01360.
    OMAi DVQHLTV.
    OrthoDBi EOG7BW0JD.
    TreeFami TF314277.

    Enzyme and pathway databases

    Reactomei REACT_206713. NGF processing.
    REACT_207837. Insulin processing.

    Miscellaneous databases

    NextBioi 294342.
    PMAP-CutDB P21661.
    PROi P21661.
    SOURCEi Search...

    Gene expression databases

    Bgeei P21661.
    CleanExi MM_PCSK2.
    Genevestigatori P21661.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    3.40.50.200. 1 hit.
    InterProi IPR008979. Galactose-bd-like.
    IPR000209. Peptidase_S8/S53_dom.
    IPR023827. Peptidase_S8_Asp-AS.
    IPR022398. Peptidase_S8_His-AS.
    IPR023828. Peptidase_S8_Ser-AS.
    IPR015500. Peptidase_S8_subtilisin-rel.
    IPR009020. Prot_inh_propept.
    IPR002884. PrprotnconvertsP.
    [Graphical view ]
    PANTHERi PTHR10795. PTHR10795. 1 hit.
    Pfami PF01483. P_proprotein. 1 hit.
    PF00082. Peptidase_S8. 1 hit.
    [Graphical view ]
    PRINTSi PR00723. SUBTILISIN.
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF52743. SSF52743. 1 hit.
    SSF54897. SSF54897. 1 hit.
    PROSITEi PS00136. SUBTILASE_ASP. 1 hit.
    PS00137. SUBTILASE_HIS. 1 hit.
    PS00138. SUBTILASE_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA sequence of two distinct pituitary proteins homologous to Kex2 and furin gene products: tissue-specific mRNAs encoding candidates for pro-hormone processing proteinases."
      Seidah N.G., Gaspar L., Mion P., Marcinkiewicz M., Mbikay M., Chretien M.
      DNA Cell Biol. 9:415-424(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pituitary.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiNEC2_MOUSE
    AccessioniPrimary (citable) accession number: P21661
    Secondary accession number(s): Q80WU1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: May 1, 1991
    Last modified: October 1, 2014
    This is version 138 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3