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P21661 (NEC2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuroendocrine convertase 2

Short name=NEC 2
EC=3.4.21.94
Alternative name(s):
KEX2-like endoprotease 2
Prohormone convertase 2
Proprotein convertase 2
Short name=PC2
Gene names
Name:Pcsk2
Synonyms:Nec-2, Nec2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length637 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Responsible for the release of glucagon from proglucagon in pancreatic A cells By similarity.

Catalytic activity

Release of protein hormones and neuropeptides from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

Subcellular location

Cytoplasmic vesiclesecretory vesicle. Note: Localized in the secretion granules.

Sequence similarities

Belongs to the peptidase S8 family. Furin subfamily.

Ontologies

Keywords
   Cellular componentCytoplasmic vesicle
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMCleavage on pair of basic residues
Disulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processembryo development

Inferred from electronic annotation. Source: Ensembl

enkephalin processing

Inferred from direct assay PubMed 8262946. Source: BHF-UCL

insulin processing

Inferred from electronic annotation. Source: Ensembl

islet amyloid polypeptide processing

Inferred from electronic annotation. Source: Ensembl

nervous system development

Inferred from expression pattern PubMed 16893422. Source: HGNC

peptide hormone processing

Inferred from direct assay PubMed 9242664. Source: BHF-UCL

protein autoprocessing

Inferred from direct assay PubMed 16893422. Source: HGNC

protein processing

Inferred from direct assay PubMed 9242664. Source: BHF-UCL

proteolysis

Inferred from mutant phenotype PubMed 12859669. Source: MGI

   Cellular_componentdendrite

Inferred from electronic annotation. Source: Ensembl

endoplasmic reticulum lumen

Traceable author statement. Source: Reactome

extracellular space

Inferred from direct assay PubMed 8397508. Source: BHF-UCL

membrane

Inferred from electronic annotation. Source: Ensembl

perikaryon

Inferred from electronic annotation. Source: Ensembl

transport vesicle

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionendopeptidase activity

Inferred from direct assay PubMed 9242664. Source: BHF-UCL

serine-type endopeptidase activity

Inferred from direct assay PubMed 8262946. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Propeptide25 – 10884 Potential
PRO_0000027067
Chain109 – 637529Neuroendocrine convertase 2
PRO_0000027068

Regions

Region121 – 414294Catalytic

Sites

Active site1661Charge relay system By similarity
Active site2071Charge relay system By similarity
Active site3831Charge relay system By similarity

Amino acid modifications

Glycosylation3741N-linked (GlcNAc...) Potential
Glycosylation5131N-linked (GlcNAc...) Potential
Glycosylation5231N-linked (GlcNAc...) Potential
Disulfide bond224 ↔ 375 By similarity
Disulfide bond316 ↔ 346 By similarity
Disulfide bond467 ↔ 493 By similarity

Sequences

Sequence LengthMass (Da)Tools
P21661 [UniParc].

Last modified May 1, 1991. Version 1.
Checksum: B69F2DCCD00FB0E6

FASTA63770,785
        10         20         30         40         50         60 
MEGGCGSQWK AAGFLFCVMV FASAERPVFT NHFLVELHKD GEEEARQVAA EHGFGVRKLP 

        70         80         90        100        110        120 
FAEGLYHFYH NGLAKAKRRR SLHHKRQLER DPRIKMALQQ EGFDRKKRGY RDINEIDINM 

       130        140        150        160        170        180 
NDPLFTKQWY LFNTGQADGT PGLDLNVAEA WELGYTGKGV TIGIMDDGID YLHPDLAYNY 

       190        200        210        220        230        240 
NADASYDFSS NDPYPYPRYT DDWFNSHGTR CAGEVSAAAS NNICGVGVAY NSKVAGIRML 

       250        260        270        280        290        300 
DQPFMTDIIE ASSISHMPQL IDIYSASWGP TDNGKTVDGP RELTLQAMAD GVNKGRGGKG 

       310        320        330        340        350        360 
SIYVWASGDG GSYDDCNCDG YASSMWTISI NSAINDGRTA LYDESCSSTL ASTFSNGRKR 

       370        380        390        400        410        420 
NPEAGVATTD LYGNCTLRHS GTSAAAPEAA GVFALALEAN LDLTWRDMQH LTVLTSKRNQ 

       430        440        450        460        470        480 
LHDEVHQWRR NGVGLEFNHL FGYGVLDAGA MVKMAKDWKT VPERFHCVGG SVQNPEKIPP 

       490        500        510        520        530        540 
TGKLVLTLKT NACEGKENFV RYLEHVQAVI TVNATRRGDL NINMTSPMGT KSILLSRRPR 

       550        560        570        580        590        600 
DDDSKVGFDK WPFMTTHTWG EDARGTWTLE LGFVGSAPQK GLLKEWTLML HGTQSAPYID 

       610        620        630 
QVVRDYQSKL AMSKKQELEE ELDEAVERSL QSILRKN 

« Hide

References

« Hide 'large scale' references
[1]"cDNA sequence of two distinct pituitary proteins homologous to Kex2 and furin gene products: tissue-specific mRNAs encoding candidates for pro-hormone processing proteinases."
Seidah N.G., Gaspar L., Mion P., Marcinkiewicz M., Mbikay M., Chretien M.
DNA Cell Biol. 9:415-424(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pituitary.
[2]Erratum
Seidah N.G., Gaspar L., Mion P., Marcinkiewicz M., Mbikay M., Chretien M.
DNA Cell Biol. 9:789-789(1990) [PubMed] [Europe PMC] [Abstract]
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[4]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M55669 mRNA. Translation: AAA39376.1.
BC052013 mRNA. Translation: AAH52013.2.
BC057348 mRNA. Translation: AAH57348.1.
PIRKXMSC2. B35571.
RefSeqNP_032818.1. NM_008792.4.
UniGeneMm.294493.
Mm.447466.

3D structure databases

ProteinModelPortalP21661.
SMRP21661. Positions 29-101, 122-593.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP21661. 1 interaction.
MINTMINT-1508709.

Protein family/group databases

MEROPSS08.073.

Proteomic databases

PaxDbP21661.
PRIDEP21661.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028905; ENSMUSP00000028905; ENSMUSG00000027419.
GeneID18549.
KEGGmmu:18549.
UCSCuc008mqf.2. mouse.

Organism-specific databases

CTD5126.
MGIMGI:97512. Pcsk2.

Phylogenomic databases

eggNOGCOG4935.
HOVERGENHBG008705.
InParanoidP21661.
KOK01360.
OMAYHCEAGE.
OrthoDBEOG7BW0JD.
TreeFamTF314277.

Enzyme and pathway databases

ReactomeREACT_188257. Signal Transduction.

Gene expression databases

BgeeP21661.
CleanExMM_PCSK2.
GenevestigatorP21661.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
[Graphical view]
PANTHERPTHR10795. PTHR10795. 1 hit.
PfamPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSPR00723. SUBTILISIN.
SUPFAMSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio294342.
PMAP-CutDBP21661.
PROP21661.
SOURCESearch...

Entry information

Entry nameNEC2_MOUSE
AccessionPrimary (citable) accession number: P21661
Secondary accession number(s): Q80WU1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: April 16, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot