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P21661

- NEC2_MOUSE

UniProt

P21661 - NEC2_MOUSE

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Protein

Neuroendocrine convertase 2

Gene
Pcsk2, Nec-2, Nec2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Responsible for the release of glucagon from proglucagon in pancreatic A cells By similarity.

Catalytic activityi

Release of protein hormones and neuropeptides from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei166 – 1661Charge relay system By similarity
Active sitei207 – 2071Charge relay system By similarity
Active sitei383 – 3831Charge relay system By similarity

GO - Molecular functioni

  1. endopeptidase activity Source: BHF-UCL
  2. serine-type endopeptidase activity Source: BHF-UCL

GO - Biological processi

  1. embryo development Source: Ensembl
  2. enkephalin processing Source: BHF-UCL
  3. insulin processing Source: Ensembl
  4. islet amyloid polypeptide processing Source: Ensembl
  5. nervous system development Source: HGNC
  6. peptide hormone processing Source: BHF-UCL
  7. protein autoprocessing Source: HGNC
  8. protein processing Source: BHF-UCL
  9. proteolysis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiREACT_206713. NGF processing.
REACT_207837. Insulin processing.

Protein family/group databases

MEROPSiS08.073.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroendocrine convertase 2 (EC:3.4.21.94)
Short name:
NEC 2
Alternative name(s):
KEX2-like endoprotease 2
Prohormone convertase 2
Proprotein convertase 2
Short name:
PC2
Gene namesi
Name:Pcsk2
Synonyms:Nec-2, Nec2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:97512. Pcsk2.

Subcellular locationi

Cytoplasmic vesiclesecretory vesicle
Note: Localized in the secretion granules.

GO - Cellular componenti

  1. dendrite Source: Ensembl
  2. endoplasmic reticulum lumen Source: Reactome
  3. extracellular space Source: BHF-UCL
  4. membrane Source: Ensembl
  5. perikaryon Source: Ensembl
  6. transport vesicle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed predictionAdd
BLAST
Propeptidei25 – 10884 Reviewed predictionPRO_0000027067Add
BLAST
Chaini109 – 637529Neuroendocrine convertase 2PRO_0000027068Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi224 ↔ 375 By similarity
Disulfide bondi316 ↔ 346 By similarity
Glycosylationi374 – 3741N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi467 ↔ 493 By similarity
Glycosylationi513 – 5131N-linked (GlcNAc...) Reviewed prediction
Glycosylationi523 – 5231N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP21661.
PRIDEiP21661.

Miscellaneous databases

PMAP-CutDBP21661.

Expressioni

Gene expression databases

BgeeiP21661.
CleanExiMM_PCSK2.
GenevestigatoriP21661.

Interactioni

Protein-protein interaction databases

IntActiP21661. 1 interaction.
MINTiMINT-1508709.

Structurei

3D structure databases

ProteinModelPortaliP21661.
SMRiP21661. Positions 122-593.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni121 – 414294CatalyticAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4935.
HOVERGENiHBG008705.
InParanoidiP21661.
KOiK01360.
OMAiDVQHLTV.
OrthoDBiEOG7BW0JD.
TreeFamiTF314277.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21661-1 [UniParc]FASTAAdd to Basket

« Hide

MEGGCGSQWK AAGFLFCVMV FASAERPVFT NHFLVELHKD GEEEARQVAA    50
EHGFGVRKLP FAEGLYHFYH NGLAKAKRRR SLHHKRQLER DPRIKMALQQ 100
EGFDRKKRGY RDINEIDINM NDPLFTKQWY LFNTGQADGT PGLDLNVAEA 150
WELGYTGKGV TIGIMDDGID YLHPDLAYNY NADASYDFSS NDPYPYPRYT 200
DDWFNSHGTR CAGEVSAAAS NNICGVGVAY NSKVAGIRML DQPFMTDIIE 250
ASSISHMPQL IDIYSASWGP TDNGKTVDGP RELTLQAMAD GVNKGRGGKG 300
SIYVWASGDG GSYDDCNCDG YASSMWTISI NSAINDGRTA LYDESCSSTL 350
ASTFSNGRKR NPEAGVATTD LYGNCTLRHS GTSAAAPEAA GVFALALEAN 400
LDLTWRDMQH LTVLTSKRNQ LHDEVHQWRR NGVGLEFNHL FGYGVLDAGA 450
MVKMAKDWKT VPERFHCVGG SVQNPEKIPP TGKLVLTLKT NACEGKENFV 500
RYLEHVQAVI TVNATRRGDL NINMTSPMGT KSILLSRRPR DDDSKVGFDK 550
WPFMTTHTWG EDARGTWTLE LGFVGSAPQK GLLKEWTLML HGTQSAPYID 600
QVVRDYQSKL AMSKKQELEE ELDEAVERSL QSILRKN 637
Length:637
Mass (Da):70,785
Last modified:May 1, 1991 - v1
Checksum:iB69F2DCCD00FB0E6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M55669 mRNA. Translation: AAA39376.1.
BC052013 mRNA. Translation: AAH52013.2.
BC057348 mRNA. Translation: AAH57348.1.
CCDSiCCDS16810.1.
PIRiB35571. KXMSC2.
RefSeqiNP_032818.1. NM_008792.4.
UniGeneiMm.294493.
Mm.447466.

Genome annotation databases

EnsembliENSMUST00000028905; ENSMUSP00000028905; ENSMUSG00000027419.
GeneIDi18549.
KEGGimmu:18549.
UCSCiuc008mqf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M55669 mRNA. Translation: AAA39376.1 .
BC052013 mRNA. Translation: AAH52013.2 .
BC057348 mRNA. Translation: AAH57348.1 .
CCDSi CCDS16810.1.
PIRi B35571. KXMSC2.
RefSeqi NP_032818.1. NM_008792.4.
UniGenei Mm.294493.
Mm.447466.

3D structure databases

ProteinModelPortali P21661.
SMRi P21661. Positions 122-593.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P21661. 1 interaction.
MINTi MINT-1508709.

Protein family/group databases

MEROPSi S08.073.

Proteomic databases

PaxDbi P21661.
PRIDEi P21661.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000028905 ; ENSMUSP00000028905 ; ENSMUSG00000027419 .
GeneIDi 18549.
KEGGi mmu:18549.
UCSCi uc008mqf.2. mouse.

Organism-specific databases

CTDi 5126.
MGIi MGI:97512. Pcsk2.

Phylogenomic databases

eggNOGi COG4935.
HOVERGENi HBG008705.
InParanoidi P21661.
KOi K01360.
OMAi DVQHLTV.
OrthoDBi EOG7BW0JD.
TreeFami TF314277.

Enzyme and pathway databases

Reactomei REACT_206713. NGF processing.
REACT_207837. Insulin processing.

Miscellaneous databases

NextBioi 294342.
PMAP-CutDB P21661.
PROi P21661.
SOURCEi Search...

Gene expression databases

Bgeei P21661.
CleanExi MM_PCSK2.
Genevestigatori P21661.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProi IPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
[Graphical view ]
PANTHERi PTHR10795. PTHR10795. 1 hit.
Pfami PF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view ]
PRINTSi PR00723. SUBTILISIN.
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEi PS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA sequence of two distinct pituitary proteins homologous to Kex2 and furin gene products: tissue-specific mRNAs encoding candidates for pro-hormone processing proteinases."
    Seidah N.G., Gaspar L., Mion P., Marcinkiewicz M., Mbikay M., Chretien M.
    DNA Cell Biol. 9:415-424(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pituitary.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiNEC2_MOUSE
AccessioniPrimary (citable) accession number: P21661
Secondary accession number(s): Q80WU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: September 3, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3