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Protein

Neuroendocrine convertase 2

Gene

Pcsk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Responsible for the release of glucagon from proglucagon in pancreatic A cells (By similarity).By similarity

Catalytic activityi

Release of protein hormones and neuropeptides from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei166 – 1661Charge relay systemBy similarity
Active sitei207 – 2071Charge relay systemBy similarity
Active sitei383 – 3831Charge relay systemBy similarity

GO - Molecular functioni

  1. endopeptidase activity Source: BHF-UCL
  2. serine-type endopeptidase activity Source: BHF-UCL

GO - Biological processi

  1. enkephalin processing Source: BHF-UCL
  2. insulin processing Source: MGI
  3. islet amyloid polypeptide processing Source: Ensembl
  4. nervous system development Source: HGNC
  5. peptide hormone processing Source: BHF-UCL
  6. protein autoprocessing Source: HGNC
  7. protein processing Source: BHF-UCL
  8. proteolysis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiREACT_298481. Insulin processing.
REACT_317611. NGF processing.

Protein family/group databases

MEROPSiS08.073.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroendocrine convertase 2 (EC:3.4.21.94)
Short name:
NEC 2
Alternative name(s):
KEX2-like endoprotease 2
Prohormone convertase 2
Proprotein convertase 2
Short name:
PC2
Gene namesi
Name:Pcsk2
Synonyms:Nec-2, Nec2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:97512. Pcsk2.

Subcellular locationi

  1. Cytoplasmic vesiclesecretory vesicle

  2. Note: Localized in the secretion granules.

GO - Cellular componenti

  1. dendrite Source: Ensembl
  2. endoplasmic reticulum lumen Source: Reactome
  3. extracellular space Source: BHF-UCL
  4. membrane Source: MGI
  5. perikaryon Source: Ensembl
  6. secretory granule Source: Ensembl
  7. transport vesicle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Propeptidei25 – 10884Sequence AnalysisPRO_0000027067Add
BLAST
Chaini109 – 637529Neuroendocrine convertase 2PRO_0000027068Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi224 ↔ 375By similarity
Disulfide bondi316 ↔ 346By similarity
Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi467 ↔ 493By similarity
Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi523 – 5231N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP21661.
PaxDbiP21661.
PRIDEiP21661.

Miscellaneous databases

PMAP-CutDBP21661.

Expressioni

Gene expression databases

BgeeiP21661.
CleanExiMM_PCSK2.
ExpressionAtlasiP21661. baseline and differential.
GenevestigatoriP21661.

Interactioni

Protein-protein interaction databases

IntActiP21661. 1 interaction.
MINTiMINT-1508709.

Structurei

3D structure databases

ProteinModelPortaliP21661.
SMRiP21661. Positions 122-593.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini161 – 452292Peptidase S8Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S8 family. Furin subfamily.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4935.
HOVERGENiHBG008705.
InParanoidiP21661.
KOiK01360.
OMAiYHCEAGE.
OrthoDBiEOG7BW0JD.
TreeFamiTF314277.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21661-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGGCGSQWK AAGFLFCVMV FASAERPVFT NHFLVELHKD GEEEARQVAA
60 70 80 90 100
EHGFGVRKLP FAEGLYHFYH NGLAKAKRRR SLHHKRQLER DPRIKMALQQ
110 120 130 140 150
EGFDRKKRGY RDINEIDINM NDPLFTKQWY LFNTGQADGT PGLDLNVAEA
160 170 180 190 200
WELGYTGKGV TIGIMDDGID YLHPDLAYNY NADASYDFSS NDPYPYPRYT
210 220 230 240 250
DDWFNSHGTR CAGEVSAAAS NNICGVGVAY NSKVAGIRML DQPFMTDIIE
260 270 280 290 300
ASSISHMPQL IDIYSASWGP TDNGKTVDGP RELTLQAMAD GVNKGRGGKG
310 320 330 340 350
SIYVWASGDG GSYDDCNCDG YASSMWTISI NSAINDGRTA LYDESCSSTL
360 370 380 390 400
ASTFSNGRKR NPEAGVATTD LYGNCTLRHS GTSAAAPEAA GVFALALEAN
410 420 430 440 450
LDLTWRDMQH LTVLTSKRNQ LHDEVHQWRR NGVGLEFNHL FGYGVLDAGA
460 470 480 490 500
MVKMAKDWKT VPERFHCVGG SVQNPEKIPP TGKLVLTLKT NACEGKENFV
510 520 530 540 550
RYLEHVQAVI TVNATRRGDL NINMTSPMGT KSILLSRRPR DDDSKVGFDK
560 570 580 590 600
WPFMTTHTWG EDARGTWTLE LGFVGSAPQK GLLKEWTLML HGTQSAPYID
610 620 630
QVVRDYQSKL AMSKKQELEE ELDEAVERSL QSILRKN
Length:637
Mass (Da):70,785
Last modified:May 1, 1991 - v1
Checksum:iB69F2DCCD00FB0E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55669 mRNA. Translation: AAA39376.1.
BC052013 mRNA. Translation: AAH52013.2.
BC057348 mRNA. Translation: AAH57348.1.
CCDSiCCDS16810.1.
PIRiB35571. KXMSC2.
RefSeqiNP_032818.1. NM_008792.4.
UniGeneiMm.294493.
Mm.447466.

Genome annotation databases

EnsembliENSMUST00000028905; ENSMUSP00000028905; ENSMUSG00000027419.
GeneIDi18549.
KEGGimmu:18549.
UCSCiuc008mqf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55669 mRNA. Translation: AAA39376.1.
BC052013 mRNA. Translation: AAH52013.2.
BC057348 mRNA. Translation: AAH57348.1.
CCDSiCCDS16810.1.
PIRiB35571. KXMSC2.
RefSeqiNP_032818.1. NM_008792.4.
UniGeneiMm.294493.
Mm.447466.

3D structure databases

ProteinModelPortaliP21661.
SMRiP21661. Positions 122-593.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP21661. 1 interaction.
MINTiMINT-1508709.

Protein family/group databases

MEROPSiS08.073.

Proteomic databases

MaxQBiP21661.
PaxDbiP21661.
PRIDEiP21661.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028905; ENSMUSP00000028905; ENSMUSG00000027419.
GeneIDi18549.
KEGGimmu:18549.
UCSCiuc008mqf.2. mouse.

Organism-specific databases

CTDi5126.
MGIiMGI:97512. Pcsk2.

Phylogenomic databases

eggNOGiCOG4935.
HOVERGENiHBG008705.
InParanoidiP21661.
KOiK01360.
OMAiYHCEAGE.
OrthoDBiEOG7BW0JD.
TreeFamiTF314277.

Enzyme and pathway databases

ReactomeiREACT_298481. Insulin processing.
REACT_317611. NGF processing.

Miscellaneous databases

NextBioi294342.
PMAP-CutDBP21661.
PROiP21661.
SOURCEiSearch...

Gene expression databases

BgeeiP21661.
CleanExiMM_PCSK2.
ExpressionAtlasiP21661. baseline and differential.
GenevestigatoriP21661.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA sequence of two distinct pituitary proteins homologous to Kex2 and furin gene products: tissue-specific mRNAs encoding candidates for pro-hormone processing proteinases."
    Seidah N.G., Gaspar L., Mion P., Marcinkiewicz M., Mbikay M., Chretien M.
    DNA Cell Biol. 9:415-424(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pituitary.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiNEC2_MOUSE
AccessioniPrimary (citable) accession number: P21661
Secondary accession number(s): Q80WU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: April 1, 2015
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.