P21661 (NEC2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuroendocrine convertase 2
Short name=NEC 2
EC=3.4.21.94

Alternative name(s):
KEX2-like endoprotease 2
Prohormone convertase 2
Proprotein convertase 2
Short name=PC2

Gene names

Name:	Pcsk2
Synonyms:	Nec-2, Nec2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	637 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues.
Catalytic activity	Release of protein hormones and neuropeptides from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.
Subcellular location	Cytoplasmic vesicle › secretory vesicle. Note: Localized in the secretion granules.
Sequence similarities	Belongs to the peptidase S8 family. Furin subfamily.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	enkephalin processing Inferred from direct assay. Source: BHF-UCL nervous system development Inferred from expression pattern. Source: HGNC protein autoprocessing Inferred from direct assay. Source: HGNC proteolysis Inferred from mutant phenotype. Source: MGI
Cellular component	endoplasmic reticulum lumen Traceable author statement. Source: Reactome extracellular space Inferred from direct assay. Source: BHF-UCL transport vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from direct assay. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Propeptide	25 – 108	84	Potential		PRO_0000027067
	Chain	109 – 637	529	Neuroendocrine convertase 2		PRO_0000027068
Regions
	Region	121 – 414	294	Catalytic
Sites
	Active site	166	1	Charge relay system By similarity
	Active site	207	1	Charge relay system By similarity
	Active site	383	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	374	1	N-linked (GlcNAc...) Potential
	Glycosylation	513	1	N-linked (GlcNAc...) Potential
	Glycosylation	523	1	N-linked (GlcNAc...) Potential
	Disulfide bond	224 ↔ 375		By similarity
	Disulfide bond	316 ↔ 346		By similarity
	Disulfide bond	467 ↔ 493		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21661 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: B69F2DCCD00FB0E6	FASTA	637	70,785
	10 20 30 40 50 60 MEGGCGSQWK AAGFLFCVMV FASAERPVFT NHFLVELHKD GEEEARQVAA EHGFGVRKLP 70 80 90 100 110 120 FAEGLYHFYH NGLAKAKRRR SLHHKRQLER DPRIKMALQQ EGFDRKKRGY RDINEIDINM 130 140 150 160 170 180 NDPLFTKQWY LFNTGQADGT PGLDLNVAEA WELGYTGKGV TIGIMDDGID YLHPDLAYNY 190 200 210 220 230 240 NADASYDFSS NDPYPYPRYT DDWFNSHGTR CAGEVSAAAS NNICGVGVAY NSKVAGIRML 250 260 270 280 290 300 DQPFMTDIIE ASSISHMPQL IDIYSASWGP TDNGKTVDGP RELTLQAMAD GVNKGRGGKG 310 320 330 340 350 360 SIYVWASGDG GSYDDCNCDG YASSMWTISI NSAINDGRTA LYDESCSSTL ASTFSNGRKR 370 380 390 400 410 420 NPEAGVATTD LYGNCTLRHS GTSAAAPEAA GVFALALEAN LDLTWRDMQH LTVLTSKRNQ 430 440 450 460 470 480 LHDEVHQWRR NGVGLEFNHL FGYGVLDAGA MVKMAKDWKT VPERFHCVGG SVQNPEKIPP 490 500 510 520 530 540 TGKLVLTLKT NACEGKENFV RYLEHVQAVI TVNATRRGDL NINMTSPMGT KSILLSRRPR 550 560 570 580 590 600 DDDSKVGFDK WPFMTTHTWG EDARGTWTLE LGFVGSAPQK GLLKEWTLML HGTQSAPYID 610 620 630 QVVRDYQSKL AMSKKQELEE ELDEAVERSL QSILRKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA sequence of two distinct pituitary proteins homologous to Kex2 and furin gene products: tissue-specific mRNAs encoding candidates for pro-hormone processing proteinases." Seidah N.G., Gaspar L., Mion P., Marcinkiewicz M., Mbikay M., Chretien M. DNA Cell Biol. 9:415-424(1990) [PubMed: 2169760] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pituitary.
[2]	Erratum Seidah N.G., Gaspar L., Mion P., Marcinkiewicz M., Mbikay M., Chretien M. DNA Cell Biol. 9:789-789(1990) [PubMed: 2264933] [Abstract]
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M55669 mRNA. Translation: AAA39376.1. BC052013 mRNA. Translation: AAH52013.2. BC057348 mRNA. Translation: AAH57348.1.
IPI	IPI00126390.
PIR	KXMSC2. B35571.
RefSeq	NP_032818.1. NM_008792.4.
UniGene	Mm.294493. Mm.447466.
3D structure databases
ProteinModelPortal	P21661.
SMR	P21661. Positions 29-101, 118-596.
ModBase	Search...
Protein-protein interaction databases
STRING	P21661.
Proteomic databases
PRIDE	P21661.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000028905; ENSMUSP00000028905; ENSMUSG00000027419.
GeneID	18549.
KEGG	mmu:18549.
Organism-specific databases
CTD	5126.
MGI	MGI:97512. Pcsk2.
Phylogenomic databases
eggNOG	roNOG07042.
HOVERGEN	HBG008705.
InParanoid	P21661.
OMA	NYNADAS.
OrthoDB	EOG46MBJ1.
PhylomeDB	P21661.
Enzyme and pathway databases
Reactome	REACT_115202. Signal Transduction.
Gene expression databases
ArrayExpress	P21661.
Bgee	P21661.
CleanEx	MM_PCSK2.
Genevestigator	P21661.
GermOnline	ENSMUSG00000027419. Mus musculus.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR000209. Peptidase_S8/S53. IPR023827. Peptidase_S8_Asp-AS. IPR022398. Peptidase_S8_His-AS. IPR023828. Peptidase_S8_Ser-AS. IPR015500. Peptidase_S8_subtilisin-rel. IPR009020. Prot_inh_propept. IPR002884. PrprotnconvertsP. [Graphical view]
Gene3D	G3DSA:3.40.50.200. Pept_S8_S53. 1 hit.
KO	K01360.
PANTHER	PTHR10795. SubtilSerProt. 1 hit.
Pfam	PF01483. P_proprotein. 1 hit. PF00082. Peptidase_S8. 1 hit. [Graphical view]
PRINTS	PR00723. SUBTILISIN.
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF52743. Pept_S8_S53. 1 hit. SSF54897. Prot_inh_propept. 1 hit.
PROSITE	PS00136. SUBTILASE_ASP. 1 hit. PS00137. SUBTILASE_HIS. 1 hit. PS00138. SUBTILASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	294342.
PMAP-CutDB	P21661.
SOURCE	Search...

Entry information

Entry name

NEC2_MOUSE

Accession

Primary (citable) accession number: P21661
Secondary accession number(s): Q80WU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	November 16, 2011
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program