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P21657

- DAL81_YEAST

UniProt

P21657 - DAL81_YEAST

Protein

Transcriptional activator protein DAL81

Gene

DAL81

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 3 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Positive regulation of genes required for catabolism of GABA (UGA4, UGA1, and UGA2), urea (DUR1 and DUR2), arginine and allantoin.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi150 – 17930Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly Source: SGD
    3. RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription Source: SGD
    4. RNA polymerase II transcription factor recruiting transcription factor activity Source: SGD
    5. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: InterPro
    6. zinc ion binding Source: InterPro

    GO - Biological processi

    1. nitrogen catabolite activation of transcription from RNA polymerase II promoter Source: SGD
    2. positive regulation of gamma-aminobutyric acid catabolic process Source: SGD
    3. positive regulation of transcription from RNA polymerase II promoter Source: GOC
    4. positive regulation of urea catabolic process Source: SGD
    5. RNA polymerase II transcriptional preinitiation complex assembly Source: GOC
    6. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31442-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional activator protein DAL81
    Alternative name(s):
    Regulatory protein UGA35
    Gene namesi
    Name:DAL81
    Synonyms:DURL, UGA35
    Ordered Locus Names:YIR023W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIR023w.
    SGDiS000001462. DAL81.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 970970Transcriptional activator protein DAL81PRO_0000114947Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei833 – 8331Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP21657.
    PaxDbiP21657.

    Expressioni

    Gene expression databases

    GenevestigatoriP21657.

    Interactioni

    Protein-protein interaction databases

    BioGridi35014. 90 interactions.
    DIPiDIP-5733N.
    IntActiP21657. 4 interactions.
    MINTiMINT-4490459.
    STRINGi4932.YIR023W.

    Structurei

    3D structure databases

    ProteinModelPortaliP21657.
    SMRiP21657. Positions 148-182.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi24 – 5027Asn-richAdd
    BLAST
    Compositional biasi73 – 8816Poly-GlnAdd
    BLAST
    Compositional biasi127 – 13913Asn-richAdd
    BLAST
    Compositional biasi227 – 23711Poly-GlnAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG79338.
    HOGENOMiHOG000094358.
    OMAiVEIFFKL.
    OrthoDBiEOG7N9044.

    Family and domain databases

    Gene3Di4.10.240.10. 1 hit.
    InterProiIPR007219. Transcription_factor_dom_fun.
    IPR001138. Zn2-C6_fun-type_DNA-bd.
    [Graphical view]
    PfamiPF04082. Fungal_trans. 1 hit.
    PF00172. Zn_clus. 1 hit.
    [Graphical view]
    SMARTiSM00906. Fungal_trans. 1 hit.
    SM00066. GAL4. 1 hit.
    [Graphical view]
    SUPFAMiSSF57701. SSF57701. 1 hit.
    PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
    PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21657-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPHQSPADN AASPTKSVKA TTKNSSTNNN VNSNNSNNNS NHDILNFNDN    50
    YTTILQHLAN DHPNILREKG GSQQQQHQQQ QQQQQQQQQQ QQQQSLDTLL 100
    HHYQSLLSKS DNAIAFDDNV SNSADHNGSN SNNNNNNNDI SSPGNLMGSC 150
    NQCRLKKTKC NYFPDLGNCL ECETSRTKCT FSIAPNYLKR TSSGANNNMP 200
    TSSNSKRMKN FEDYSNRLPS SMLYRHQQQQ QQQQQQQRIQ YPRSSFFVGP 250
    ASVFDLNLTK HVRLDNVDQI QLSKTLSLRK VSPTAQFILQ DDFDTTLHSK 300
    QEYEVDLVEN LVHPHGHLLV EIFFKLIHPF LPILHERVFL EKYSRSYREL 350
    TAPLLASIYS LALQYWDFHP ALLGFPKPDV TAQLNNIALE TFYARVGRPK 400
    LSIIQTGLLI LQCRSECHNN WVLCSSVVAL AEELGLGVEC NDWKLPKWEK 450
    DLRKRLAWAV WLMDKWCALN EGRQSHLILG RNWMIKLLNF DDFPLNSPTI 500
    LNSLQNDQSG SSPSSSNDVK NHQIAFGNLP IFNINPTLED FKNGTLMFQQ 550
    MVSLSIILGE IMDTFYTQGS MTINKSIEQV LKLAKPLQLK LREWYHSLPK 600
    NLSMSYATPQ KLNSNSTLTL AYFATEITLH RKIICALNPQ TPKELVQVCR 650
    TAARTRLVAA IEFIRDLKNE HINAFWYNCS TGNLMLIGTF AALLYVTSAT 700
    KEEAMIFRDY VRNYTWVLKI GSKYFDKLSN ALNNMHLLFA QIPGLLTDEP 750
    VVVSPNSNIN SVNPQRSGVQ SQIPIQFNVG SPAMTEQGSP LNQWKNLPQE 800
    ILQQLNSFPN GTTSTTTPVN PTSRQTQLES QGSPAINSAN NNSNNTPLPF 850
    APNKSSKKTS QSSPNVTPSH MSRHPPSNTS SPRVNSSTNV NSNTQMNASP 900
    LTSINETRQE SGDAADEKTA GRERTANEES STELKDDNPN SNQETSATGN 950
    QTIKMNDDKN VTINTRETPL 970
    Length:970
    Mass (Da):109,174
    Last modified:February 1, 1995 - v3
    Checksum:i9FEBD271A18AFC38
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti77 – 826Missing in AAA35192. (PubMed:1999281)Curated
    Sequence conflicti465 – 4651K → R in AAA35192. (PubMed:1999281)Curated
    Sequence conflicti497 – 4971S → L in AAA35192. (PubMed:1999281)Curated
    Sequence conflicti507 – 5071D → G in AAA35192. (PubMed:1999281)Curated
    Sequence conflicti760 – 7601N → S in AAA35192. (PubMed:1999281)Curated
    Sequence conflicti785 – 7851T → A in AAA34557. (PubMed:1990272)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63498 Genomic DNA. Translation: AAA35192.1.
    M60415 Genomic DNA. Translation: AAA34557.1.
    Z38061 Genomic DNA. Translation: CAA86183.1.
    BK006942 Genomic DNA. Translation: DAA08570.1.
    PIRiS48485.
    RefSeqiNP_012289.3. NM_001179545.3.

    Genome annotation databases

    EnsemblFungiiYIR023W; YIR023W; YIR023W.
    GeneIDi854841.
    KEGGisce:YIR023W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63498 Genomic DNA. Translation: AAA35192.1 .
    M60415 Genomic DNA. Translation: AAA34557.1 .
    Z38061 Genomic DNA. Translation: CAA86183.1 .
    BK006942 Genomic DNA. Translation: DAA08570.1 .
    PIRi S48485.
    RefSeqi NP_012289.3. NM_001179545.3.

    3D structure databases

    ProteinModelPortali P21657.
    SMRi P21657. Positions 148-182.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35014. 90 interactions.
    DIPi DIP-5733N.
    IntActi P21657. 4 interactions.
    MINTi MINT-4490459.
    STRINGi 4932.YIR023W.

    Proteomic databases

    MaxQBi P21657.
    PaxDbi P21657.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIR023W ; YIR023W ; YIR023W .
    GeneIDi 854841.
    KEGGi sce:YIR023W.

    Organism-specific databases

    CYGDi YIR023w.
    SGDi S000001462. DAL81.

    Phylogenomic databases

    eggNOGi NOG79338.
    HOGENOMi HOG000094358.
    OMAi VEIFFKL.
    OrthoDBi EOG7N9044.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31442-MONOMER.

    Miscellaneous databases

    NextBioi 977722.

    Gene expression databases

    Genevestigatori P21657.

    Family and domain databases

    Gene3Di 4.10.240.10. 1 hit.
    InterProi IPR007219. Transcription_factor_dom_fun.
    IPR001138. Zn2-C6_fun-type_DNA-bd.
    [Graphical view ]
    Pfami PF04082. Fungal_trans. 1 hit.
    PF00172. Zn_clus. 1 hit.
    [Graphical view ]
    SMARTi SM00906. Fungal_trans. 1 hit.
    SM00066. GAL4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57701. SSF57701. 1 hit.
    PROSITEi PS00463. ZN2_CY6_FUNGAL_1. 1 hit.
    PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The pleiotropic UGA35(DURL) regulatory gene of Saccharomyces cerevisiae: cloning, sequence and identity with the DAL81 gene."
      Coornaert D., Vissers S., Andre B.
      Gene 97:163-171(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The DAL81 gene product is required for induced expression of two differently regulated nitrogen catabolic genes in Saccharomyces cerevisiae."
      Bricmont P.A., Daugherty J.R., Cooper T.G.
      Mol. Cell. Biol. 11:1161-1166(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-833, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDAL81_YEAST
    AccessioniPrimary (citable) accession number: P21657
    Secondary accession number(s): D6VVV4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 129 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 704 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3