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P21657

- DAL81_YEAST

UniProt

P21657 - DAL81_YEAST

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Protein

Transcriptional activator protein DAL81

Gene

DAL81

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Positive regulation of genes required for catabolism of GABA (UGA4, UGA1, and UGA2), urea (DUR1 and DUR2), arginine and allantoin.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi150 – 17930Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly Source: SGD
  3. RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription Source: SGD
  4. RNA polymerase II transcription factor recruiting transcription factor activity Source: SGD
  5. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: InterPro
  6. zinc ion binding Source: InterPro

GO - Biological processi

  1. nitrogen catabolite activation of transcription from RNA polymerase II promoter Source: SGD
  2. positive regulation of gamma-aminobutyric acid catabolic process Source: SGD
  3. positive regulation of transcription from RNA polymerase II promoter Source: GOC
  4. positive regulation of urea catabolic process Source: SGD
  5. RNA polymerase II transcriptional preinitiation complex assembly Source: GOC
  6. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31442-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator protein DAL81
Alternative name(s):
Regulatory protein UGA35
Gene namesi
Name:DAL81
Synonyms:DURL, UGA35
Ordered Locus Names:YIR023W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIR023w.
SGDiS000001462. DAL81.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 970970Transcriptional activator protein DAL81PRO_0000114947Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei833 – 8331Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP21657.
PaxDbiP21657.

Expressioni

Gene expression databases

GenevestigatoriP21657.

Interactioni

Protein-protein interaction databases

BioGridi35014. 91 interactions.
DIPiDIP-5733N.
IntActiP21657. 4 interactions.
MINTiMINT-4490459.
STRINGi4932.YIR023W.

Structurei

3D structure databases

ProteinModelPortaliP21657.
SMRiP21657. Positions 148-182.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi24 – 5027Asn-richAdd
BLAST
Compositional biasi73 – 8816Poly-GlnAdd
BLAST
Compositional biasi127 – 13913Asn-richAdd
BLAST
Compositional biasi227 – 23711Poly-GlnAdd
BLAST

Sequence similaritiesi

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG79338.
HOGENOMiHOG000094358.
InParanoidiP21657.
OMAiVEIFFKL.
OrthoDBiEOG7N9044.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR007219. Transcription_factor_dom_fun.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF04082. Fungal_trans. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00906. Fungal_trans. 1 hit.
SM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21657-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDPHQSPADN AASPTKSVKA TTKNSSTNNN VNSNNSNNNS NHDILNFNDN
60 70 80 90 100
YTTILQHLAN DHPNILREKG GSQQQQHQQQ QQQQQQQQQQ QQQQSLDTLL
110 120 130 140 150
HHYQSLLSKS DNAIAFDDNV SNSADHNGSN SNNNNNNNDI SSPGNLMGSC
160 170 180 190 200
NQCRLKKTKC NYFPDLGNCL ECETSRTKCT FSIAPNYLKR TSSGANNNMP
210 220 230 240 250
TSSNSKRMKN FEDYSNRLPS SMLYRHQQQQ QQQQQQQRIQ YPRSSFFVGP
260 270 280 290 300
ASVFDLNLTK HVRLDNVDQI QLSKTLSLRK VSPTAQFILQ DDFDTTLHSK
310 320 330 340 350
QEYEVDLVEN LVHPHGHLLV EIFFKLIHPF LPILHERVFL EKYSRSYREL
360 370 380 390 400
TAPLLASIYS LALQYWDFHP ALLGFPKPDV TAQLNNIALE TFYARVGRPK
410 420 430 440 450
LSIIQTGLLI LQCRSECHNN WVLCSSVVAL AEELGLGVEC NDWKLPKWEK
460 470 480 490 500
DLRKRLAWAV WLMDKWCALN EGRQSHLILG RNWMIKLLNF DDFPLNSPTI
510 520 530 540 550
LNSLQNDQSG SSPSSSNDVK NHQIAFGNLP IFNINPTLED FKNGTLMFQQ
560 570 580 590 600
MVSLSIILGE IMDTFYTQGS MTINKSIEQV LKLAKPLQLK LREWYHSLPK
610 620 630 640 650
NLSMSYATPQ KLNSNSTLTL AYFATEITLH RKIICALNPQ TPKELVQVCR
660 670 680 690 700
TAARTRLVAA IEFIRDLKNE HINAFWYNCS TGNLMLIGTF AALLYVTSAT
710 720 730 740 750
KEEAMIFRDY VRNYTWVLKI GSKYFDKLSN ALNNMHLLFA QIPGLLTDEP
760 770 780 790 800
VVVSPNSNIN SVNPQRSGVQ SQIPIQFNVG SPAMTEQGSP LNQWKNLPQE
810 820 830 840 850
ILQQLNSFPN GTTSTTTPVN PTSRQTQLES QGSPAINSAN NNSNNTPLPF
860 870 880 890 900
APNKSSKKTS QSSPNVTPSH MSRHPPSNTS SPRVNSSTNV NSNTQMNASP
910 920 930 940 950
LTSINETRQE SGDAADEKTA GRERTANEES STELKDDNPN SNQETSATGN
960 970
QTIKMNDDKN VTINTRETPL
Length:970
Mass (Da):109,174
Last modified:February 1, 1995 - v3
Checksum:i9FEBD271A18AFC38
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 826Missing in AAA35192. (PubMed:1999281)Curated
Sequence conflicti465 – 4651K → R in AAA35192. (PubMed:1999281)Curated
Sequence conflicti497 – 4971S → L in AAA35192. (PubMed:1999281)Curated
Sequence conflicti507 – 5071D → G in AAA35192. (PubMed:1999281)Curated
Sequence conflicti760 – 7601N → S in AAA35192. (PubMed:1999281)Curated
Sequence conflicti785 – 7851T → A in AAA34557. (PubMed:1990272)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63498 Genomic DNA. Translation: AAA35192.1.
M60415 Genomic DNA. Translation: AAA34557.1.
Z38061 Genomic DNA. Translation: CAA86183.1.
BK006942 Genomic DNA. Translation: DAA08570.1.
PIRiS48485.
RefSeqiNP_012289.3. NM_001179545.3.

Genome annotation databases

EnsemblFungiiYIR023W; YIR023W; YIR023W.
GeneIDi854841.
KEGGisce:YIR023W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63498 Genomic DNA. Translation: AAA35192.1 .
M60415 Genomic DNA. Translation: AAA34557.1 .
Z38061 Genomic DNA. Translation: CAA86183.1 .
BK006942 Genomic DNA. Translation: DAA08570.1 .
PIRi S48485.
RefSeqi NP_012289.3. NM_001179545.3.

3D structure databases

ProteinModelPortali P21657.
SMRi P21657. Positions 148-182.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35014. 91 interactions.
DIPi DIP-5733N.
IntActi P21657. 4 interactions.
MINTi MINT-4490459.
STRINGi 4932.YIR023W.

Proteomic databases

MaxQBi P21657.
PaxDbi P21657.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIR023W ; YIR023W ; YIR023W .
GeneIDi 854841.
KEGGi sce:YIR023W.

Organism-specific databases

CYGDi YIR023w.
SGDi S000001462. DAL81.

Phylogenomic databases

eggNOGi NOG79338.
HOGENOMi HOG000094358.
InParanoidi P21657.
OMAi VEIFFKL.
OrthoDBi EOG7N9044.

Enzyme and pathway databases

BioCyci YEAST:G3O-31442-MONOMER.

Miscellaneous databases

NextBioi 977722.

Gene expression databases

Genevestigatori P21657.

Family and domain databases

Gene3Di 4.10.240.10. 1 hit.
InterProi IPR007219. Transcription_factor_dom_fun.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view ]
Pfami PF04082. Fungal_trans. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view ]
SMARTi SM00906. Fungal_trans. 1 hit.
SM00066. GAL4. 1 hit.
[Graphical view ]
SUPFAMi SSF57701. SSF57701. 1 hit.
PROSITEi PS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The pleiotropic UGA35(DURL) regulatory gene of Saccharomyces cerevisiae: cloning, sequence and identity with the DAL81 gene."
    Coornaert D., Vissers S., Andre B.
    Gene 97:163-171(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The DAL81 gene product is required for induced expression of two differently regulated nitrogen catabolic genes in Saccharomyces cerevisiae."
    Bricmont P.A., Daugherty J.R., Cooper T.G.
    Mol. Cell. Biol. 11:1161-1166(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-833, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDAL81_YEAST
AccessioniPrimary (citable) accession number: P21657
Secondary accession number(s): D6VVV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 704 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3