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P21657 (DAL81_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional activator protein DAL81
Alternative name(s):
Regulatory protein UGA35
Gene names
Name:DAL81
Synonyms:DURL, UGA35
Ordered Locus Names:YIR023W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length970 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Positive regulation of genes required for catabolism of GABA (UGA4, UGA1, and UGA2), urea (DUR1 and DUR2), arginine and allantoin.

Subcellular location

Nucleus.

Miscellaneous

Present with 704 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA polymerase II transcriptional preinitiation complex assembly

Inferred from direct assay PubMed 21515579. Source: GOC

nitrogen catabolite activation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 7899074. Source: SGD

positive regulation of gamma-aminobutyric acid catabolic process

Inferred from mutant phenotype PubMed 2406136. Source: SGD

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 17603098. Source: GOC

positive regulation of urea catabolic process

Inferred from mutant phenotype PubMed 2406136. Source: SGD

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 21515579. Source: GOC

   Cellular_componentnucleus

Inferred by curator PubMed 20581295. Source: SGD

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly

Inferred from direct assay PubMed 21515579. Source: SGD

RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription

Inferred from mutant phenotype PubMed 17603098. Source: SGD

RNA polymerase II transcription factor recruiting transcription factor activity

Inferred from direct assay PubMed 21515579. Source: SGD

sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 970970Transcriptional activator protein DAL81
PRO_0000114947

Regions

DNA binding150 – 17930Zn(2)-C6 fungal-type
Compositional bias24 – 5027Asn-rich
Compositional bias73 – 8816Poly-Gln
Compositional bias127 – 13913Asn-rich
Compositional bias227 – 23711Poly-Gln

Amino acid modifications

Modified residue8331Phosphoserine Ref.7

Experimental info

Sequence conflict77 – 826Missing in AAA35192. Ref.1
Sequence conflict4651K → R in AAA35192. Ref.1
Sequence conflict4971S → L in AAA35192. Ref.1
Sequence conflict5071D → G in AAA35192. Ref.1
Sequence conflict7601N → S in AAA35192. Ref.1
Sequence conflict7851T → A in AAA34557. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P21657 [UniParc].

Last modified February 1, 1995. Version 3.
Checksum: 9FEBD271A18AFC38

FASTA970109,174
        10         20         30         40         50         60 
MDPHQSPADN AASPTKSVKA TTKNSSTNNN VNSNNSNNNS NHDILNFNDN YTTILQHLAN 

        70         80         90        100        110        120 
DHPNILREKG GSQQQQHQQQ QQQQQQQQQQ QQQQSLDTLL HHYQSLLSKS DNAIAFDDNV 

       130        140        150        160        170        180 
SNSADHNGSN SNNNNNNNDI SSPGNLMGSC NQCRLKKTKC NYFPDLGNCL ECETSRTKCT 

       190        200        210        220        230        240 
FSIAPNYLKR TSSGANNNMP TSSNSKRMKN FEDYSNRLPS SMLYRHQQQQ QQQQQQQRIQ 

       250        260        270        280        290        300 
YPRSSFFVGP ASVFDLNLTK HVRLDNVDQI QLSKTLSLRK VSPTAQFILQ DDFDTTLHSK 

       310        320        330        340        350        360 
QEYEVDLVEN LVHPHGHLLV EIFFKLIHPF LPILHERVFL EKYSRSYREL TAPLLASIYS 

       370        380        390        400        410        420 
LALQYWDFHP ALLGFPKPDV TAQLNNIALE TFYARVGRPK LSIIQTGLLI LQCRSECHNN 

       430        440        450        460        470        480 
WVLCSSVVAL AEELGLGVEC NDWKLPKWEK DLRKRLAWAV WLMDKWCALN EGRQSHLILG 

       490        500        510        520        530        540 
RNWMIKLLNF DDFPLNSPTI LNSLQNDQSG SSPSSSNDVK NHQIAFGNLP IFNINPTLED 

       550        560        570        580        590        600 
FKNGTLMFQQ MVSLSIILGE IMDTFYTQGS MTINKSIEQV LKLAKPLQLK LREWYHSLPK 

       610        620        630        640        650        660 
NLSMSYATPQ KLNSNSTLTL AYFATEITLH RKIICALNPQ TPKELVQVCR TAARTRLVAA 

       670        680        690        700        710        720 
IEFIRDLKNE HINAFWYNCS TGNLMLIGTF AALLYVTSAT KEEAMIFRDY VRNYTWVLKI 

       730        740        750        760        770        780 
GSKYFDKLSN ALNNMHLLFA QIPGLLTDEP VVVSPNSNIN SVNPQRSGVQ SQIPIQFNVG 

       790        800        810        820        830        840 
SPAMTEQGSP LNQWKNLPQE ILQQLNSFPN GTTSTTTPVN PTSRQTQLES QGSPAINSAN 

       850        860        870        880        890        900 
NNSNNTPLPF APNKSSKKTS QSSPNVTPSH MSRHPPSNTS SPRVNSSTNV NSNTQMNASP 

       910        920        930        940        950        960 
LTSINETRQE SGDAADEKTA GRERTANEES STELKDDNPN SNQETSATGN QTIKMNDDKN 

       970 
VTINTRETPL 

« Hide

References

« Hide 'large scale' references
[1]"The pleiotropic UGA35(DURL) regulatory gene of Saccharomyces cerevisiae: cloning, sequence and identity with the DAL81 gene."
Coornaert D., Vissers S., Andre B.
Gene 97:163-171(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The DAL81 gene product is required for induced expression of two differently regulated nitrogen catabolic genes in Saccharomyces cerevisiae."
Bricmont P.A., Daugherty J.R., Cooper T.G.
Mol. Cell. Biol. 11:1161-1166(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-833, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M63498 Genomic DNA. Translation: AAA35192.1.
M60415 Genomic DNA. Translation: AAA34557.1.
Z38061 Genomic DNA. Translation: CAA86183.1.
BK006942 Genomic DNA. Translation: DAA08570.1.
PIRS48485.
RefSeqNP_012289.3. NM_001179545.3.

3D structure databases

ProteinModelPortalP21657.
SMRP21657. Positions 148-182.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35014. 89 interactions.
DIPDIP-5733N.
IntActP21657. 4 interactions.
MINTMINT-4490459.
STRING4932.YIR023W.

Proteomic databases

MaxQBP21657.
PaxDbP21657.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIR023W; YIR023W; YIR023W.
GeneID854841.
KEGGsce:YIR023W.

Organism-specific databases

CYGDYIR023w.
SGDS000001462. DAL81.

Phylogenomic databases

eggNOGNOG79338.
HOGENOMHOG000094358.
OMAVEIFFKL.
OrthoDBEOG7N9044.

Enzyme and pathway databases

BioCycYEAST:G3O-31442-MONOMER.

Gene expression databases

GenevestigatorP21657.

Family and domain databases

Gene3D4.10.240.10. 1 hit.
InterProIPR007219. Transcription_factor_dom_fun.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamPF04082. Fungal_trans. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTSM00906. Fungal_trans. 1 hit.
SM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMSSF57701. SSF57701. 1 hit.
PROSITEPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977722.

Entry information

Entry nameDAL81_YEAST
AccessionPrimary (citable) accession number: P21657
Secondary accession number(s): D6VVV4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: February 1, 1995
Last modified: July 9, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families