Transcriptional activator protein DAL81 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P21657 (DAL81_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 115. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Transcriptional activator protein DAL81

Alternative name(s):
Regulatory protein UGA35

Gene names

Name:	DAL81
Synonyms:	DURL, UGA35
Ordered Locus Names:	YIR023W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	970 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Positive regulation of genes required for catabolism of GABA (UGA4, UGA1, and UGA2), urea (DUR1 and DUR2), arginine and allantoin.
Subcellular location	Nucleus.
Miscellaneous	Present with 704 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nitrogen catabolite activation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 7899074. Source: SGD positive regulation of gamma-aminobutyric acid catabolic process Inferred from mutant phenotype PubMed 2406136. Source: SGD positive regulation of urea catabolic process Inferred from mutant phenotype PubMed 2406136. Source: SGD
Cellular_component	nucleus Inferred by curator PubMed 20581295. Source: SGD
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly Inferred from direct assay PubMed 21515579. Source: SGD RNA polymerase II transcription factor recruiting transcription factor activity Inferred from direct assay PubMed 21515579. Source: SGD sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 970

970

Transcriptional activator protein DAL81

PRO_0000114947

Regions

DNA binding

150 – 179

Zn(2)-C6 fungal-type

Compositional bias

24 – 50

Asn-rich

Compositional bias

73 – 88

Poly-Gln

Compositional bias

127 – 139

Asn-rich

Compositional bias

227 – 237

Poly-Gln

Amino acid modifications

Modified residue

191

Phosphothreonine Ref.7

Modified residue

192

Phosphoserine Ref.7

Modified residue

193

Phosphoserine Ref.7

Modified residue

205

Phosphoserine Ref.7

Modified residue

863

Phosphoserine Ref.7 Ref.8

Modified residue

877

Phosphoserine Ref.7

Modified residue

881

Phosphoserine Ref.7

Modified residue

968

Phosphothreonine Ref.6

Experimental info

Sequence conflict

77 – 82

Missing in AAA35192. Ref.1

Sequence conflict

465

K → R in AAA35192. Ref.1

Sequence conflict

497

S → L in AAA35192. Ref.1

Sequence conflict

507

D → G in AAA35192. Ref.1

Sequence conflict

760

N → S in AAA35192. Ref.1

Sequence conflict

785

T → A in AAA34557. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P21657 [UniParc].

Last modified February 1, 1995. Version 3.
Checksum: 9FEBD271A18AFC38
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FASTA

970

109,174

        10         20         30         40         50         60 
MDPHQSPADN AASPTKSVKA TTKNSSTNNN VNSNNSNNNS NHDILNFNDN YTTILQHLAN 

        70         80         90        100        110        120 
DHPNILREKG GSQQQQHQQQ QQQQQQQQQQ QQQQSLDTLL HHYQSLLSKS DNAIAFDDNV 

       130        140        150        160        170        180 
SNSADHNGSN SNNNNNNNDI SSPGNLMGSC NQCRLKKTKC NYFPDLGNCL ECETSRTKCT 

       190        200        210        220        230        240 
FSIAPNYLKR TSSGANNNMP TSSNSKRMKN FEDYSNRLPS SMLYRHQQQQ QQQQQQQRIQ 

       250        260        270        280        290        300 
YPRSSFFVGP ASVFDLNLTK HVRLDNVDQI QLSKTLSLRK VSPTAQFILQ DDFDTTLHSK 

       310        320        330        340        350        360 
QEYEVDLVEN LVHPHGHLLV EIFFKLIHPF LPILHERVFL EKYSRSYREL TAPLLASIYS 

       370        380        390        400        410        420 
LALQYWDFHP ALLGFPKPDV TAQLNNIALE TFYARVGRPK LSIIQTGLLI LQCRSECHNN 

       430        440        450        460        470        480 
WVLCSSVVAL AEELGLGVEC NDWKLPKWEK DLRKRLAWAV WLMDKWCALN EGRQSHLILG 

       490        500        510        520        530        540 
RNWMIKLLNF DDFPLNSPTI LNSLQNDQSG SSPSSSNDVK NHQIAFGNLP IFNINPTLED 

       550        560        570        580        590        600 
FKNGTLMFQQ MVSLSIILGE IMDTFYTQGS MTINKSIEQV LKLAKPLQLK LREWYHSLPK 

       610        620        630        640        650        660 
NLSMSYATPQ KLNSNSTLTL AYFATEITLH RKIICALNPQ TPKELVQVCR TAARTRLVAA 

       670        680        690        700        710        720 
IEFIRDLKNE HINAFWYNCS TGNLMLIGTF AALLYVTSAT KEEAMIFRDY VRNYTWVLKI 

       730        740        750        760        770        780 
GSKYFDKLSN ALNNMHLLFA QIPGLLTDEP VVVSPNSNIN SVNPQRSGVQ SQIPIQFNVG 

       790        800        810        820        830        840 
SPAMTEQGSP LNQWKNLPQE ILQQLNSFPN GTTSTTTPVN PTSRQTQLES QGSPAINSAN 

       850        860        870        880        890        900 
NNSNNTPLPF APNKSSKKTS QSSPNVTPSH MSRHPPSNTS SPRVNSSTNV NSNTQMNASP 

       910        920        930        940        950        960 
LTSINETRQE SGDAADEKTA GRERTANEES STELKDDNPN SNQETSATGN QTIKMNDDKN 

       970 
VTINTRETPL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The pleiotropic UGA35(DURL) regulatory gene of Saccharomyces cerevisiae: cloning, sequence and identity with the DAL81 gene." Coornaert D., Vissers S., Andre B. Gene 97:163-171(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The DAL81 gene product is required for induced expression of two differently regulated nitrogen catabolic genes in Saccharomyces cerevisiae." Bricmont P.A., Daugherty J.R., Cooper T.G. Mol. Cell. Biol. 11:1161-1166(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-968, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-191; SER-192; SER-193; SER-205; SER-863; SER-877 AND SER-881, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-863, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M63498 Genomic DNA. Translation: AAA35192.1. M60415 Genomic DNA. Translation: AAA34557.1. Z38061 Genomic DNA. Translation: CAA86183.1. BK006942 Genomic DNA. Translation: DAA08570.1.
PIR	S48485.
RefSeq	NP_012289.3. NM_001179545.3.
3D structure databases
ProteinModelPortal	P21657.
SMR	P21657. Positions 148-182.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5733N.
IntAct	P21657. 3 interactions.
MINT	MINT-4490459.
STRING	4932.YIR023W.
Proteomic databases
PaxDb	P21657.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIR023W; YIR023W; YIR023W.
GeneID	854841.
KEGG	sce:YIR023W. sce:YIR031C.
Organism-specific databases
CYGD	YIR023w.
SGD	S000001462. DAL81.
Phylogenomic databases
eggNOG	NOG79338.
HOGENOM	HOG000094358.
KO	K01638.
OMA	VEIFFKL.
OrthoDB	EOG4N33X4.
Gene expression databases
Genevestigator	P21657.
GermOnline	YIR023W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	4.10.240.10. 1 hit.
InterPro	IPR007219. Transcription_factor_fun. IPR001138. Zn2-C6_fun-type_DNA-bd. [Graphical view]
Pfam	PF04082. Fungal_trans. 1 hit. PF00172. Zn_clus. 1 hit. [Graphical view]
SMART	SM00906. Fungal_trans. 1 hit. SM00066. GAL4. 1 hit. [Graphical view]
SUPFAM	SSF57701. Fungi_TrN. 1 hit.
PROSITE	PS00463. ZN2_CY6_FUNGAL_1. 1 hit. PS50048. ZN2_CY6_FUNGAL_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977722.

Entry information

Entry name

DAL81_YEAST

Accession

Primary (citable) accession number: P21657
Secondary accession number(s): D6VVV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 115 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents