P21651 (RE107_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Recombination protein 107

Alternative name(s):
Meiotic recombination protein 2

Gene names

Name:	REC107
Synonyms:	MER2
Ordered Locus Names:	YJR021C
ORF Names:	J1462

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for meiotic chromosome segregation. MER1 and MER2 proteins must interact directly or indirectly. MER1 might be responsible for regulating the MER2 gene and/or gene product. MER2 is not required for mitosis and mitotic DNA repair mechanisms. Component of the MER2-MEI4-REC114 complex which seems to be required for meiotic double-strand break (DSB) formation. Ref.8
Subunit structure	Interacts with MEI4, REC114 and XRS2. Component of the MER2-MEI4-REC114 complex. Ref.7 Ref.8
Subcellular location	Nucleus. Chromosome. Note: Localizes to chromosomes. Ref.8
Post-translational modification	Phosphorylated during meiotic prophase. Dephosphorylation seems to be dependent on DSB formation. Ref.8

Ontologies

Keywords
Biological process	DNA recombination Meiosis
Cellular component	Chromosome Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	reciprocal meiotic recombination Inferred from mutant phenotype PubMed 8536989. Source: SGD
Cellular_component	condensed nuclear chromosome Inferred from direct assay Ref.8. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Recombination protein 107		PRO_0000096421
Experimental info
	Sequence conflict	279	1	N → I Ref.1
	Sequence conflict	279	1	N → I Ref.2
	Sequence conflict	284 – 314	31	DNDDA…LESEL → TMMTLAIG Ref.1
	Sequence conflict	284 – 314	31	DNDDA…LESEL → TMMTLAIG Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P21651 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 46F426AC0DFDFAD0	FASTA	314	35,683
	10 20 30 40 50 60 MVARGRTDEI STDVSEANSE HSLMITETSS PFRSIFSHSG KVANAGALEE SDKQILEWAG 70 80 90 100 110 120 KLELESMELR ENSDKLIKVL NENSKTLCKS LNKFNQLLEQ DAATNGNVKT LIKDLASQIE 130 140 150 160 170 180 NQLDKVSTAM LSKGDEKKTK SDSSYRQVLV EEISRYNSKI TRHVTNKQHE TEKSMRCTQE 190 200 210 220 230 240 MLFNVGSQLE DVHKVLLSLS KDMHSLQTRQ TALEMAFREK ADHAYDRPDV SLNGTTLLHD 250 260 270 280 290 300 MDEAHDKQRK KSVPPPRMMV TRSMKRRRSS SPTLSTSQNH NSEDNDDASH RLKRAARTII 310 PWEELRPDTL ESEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Meiotic gene conversion and crossing over: their relationship to each other and to chromosome synapsis and segregation." Engebrecht J., Hirsch J., Roeder G.S. Cell 62:927-937(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Meiosis-specific RNA splicing in yeast." Engebrecht J., Voelkel-Meiman K., Roeder G.S. Cell 66:1257-1268(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"PTF1 encodes an essential protein in Saccharomyces cerevisiae, which shows strong homology with a new putative family of PPIases." Hani J., Stumpf G., Domdey H. FEBS Lett. 365:198-202(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 213-314. Strain: DH484.
[6]	"Molecular and genetic analysis of the yeast early meiotic recombination genes REC102 and REC107/MER2." Cool M., Malone R.E. Mol. Cell. Biol. 12:1248-1256(1992) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF REC107 AS MER2.
[7]	"Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism." Arora C., Kee K., Maleki S., Keeney S. Mol. Cell 13:549-559(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MEI4; REC114 AND XRS2.
[8]	"Saccharomyces cerevisiae Mer2, Mei4 and Rec114 form a complex required for meiotic double-strand break formation." Li J., Hooker G.W., Roeder G.S. Genetics 173:1969-1981(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE MER2-MEI4-REC114 COMPLEX, FUNCTION OF THE MER2-MEI4-REC114 COMPLEX, INTERACTION WITH MEI4 AND REC114, SUBCELLULAR LOCATION, PHOSPHORYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M38340 Genomic DNA. Translation: AAA34772.1. M60755 mRNA. Translation: AAA34773.1. X87611 Genomic DNA. Translation: CAA60944.1. Z49521 Genomic DNA. Translation: CAA89546.1. Z49519 Genomic DNA. Translation: CAA89545.1. X85972 Genomic DNA. Translation: CAA59959.1. BK006943 Genomic DNA. Translation: DAA08811.1.
PIR	S55210.
RefSeq	NP_012555.1. NM_001181679.1.
3D structure databases
ProteinModelPortal	P21651.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5265N.
IntAct	P21651. 3 interactions.
MINT	MINT-523746.
STRING	4932.YJR021C.
Proteomic databases
PaxDb	P21651.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR021C; YJR021C; YJR021C.
GeneID	853478.
KEGG	sce:YJR021C.
Organism-specific databases
CYGD	YJR021c.
SGD	S000003782. REC107.
Phylogenomic databases
eggNOG	NOG41190.
OrthoDB	EOG4WHCWN.
Gene expression databases
Genevestigator	P21651.
GermOnline	YJR021C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015159. Meiotic_recombination. [Graphical view]
Pfam	PF09074. Mer2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974086.

Entry information

Entry name

RE107_YEAST

Accession

Primary (citable) accession number: P21651
Secondary accession number(s): D6VWJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program