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Protein

Tryptophan 2,3-dioxygenase

Gene

Tdo2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Incorporates oxygen into the indole moiety of tryptophan. Has a broad specificity towards tryptamine and derivatives including D- and L-tryptophan, 5-hydroxytryptophan and serotonin.

Catalytic activityi

L-tryptophan + O2 = N-formyl-L-kynurenine.UniRule annotation

Cofactori

hemeNote: Binds 2 heme groups per tetramer.

Pathwayi: L-tryptophan degradation via kynurenine pathway

This protein is involved in step 1 of the subpathway that synthesizes L-kynurenine from L-tryptophan.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Tryptophan 2,3-dioxygenase (Tdo2), Indoleamine 2,3-dioxygenase 2 (Ido2)
  2. Kynurenine formamidase (Afmid)
This subpathway is part of the pathway L-tryptophan degradation via kynurenine pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-kynurenine from L-tryptophan, the pathway L-tryptophan degradation via kynurenine pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei144SubstrateUniRule annotation1
Binding sitei151HemeUniRule annotation1
Metal bindingi328Iron (heme axial ligand)UniRule annotation1
Binding sitei342SubstrateUniRule annotation1

GO - Molecular functioni

  • amino acid binding Source: RGD
  • heme binding Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • oxygen binding Source: RGD
  • tryptophan 2,3-dioxygenase activity Source: RGD

GO - Biological processi

  • tryptophan catabolic process to acetyl-CoA Source: RGD
  • tryptophan catabolic process to kynurenine Source: UniProtKB-UniPathway
  • tryptophan metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Tryptophan catabolism

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.11. 5301.
ReactomeiR-RNO-71240. Tryptophan catabolism.
UniPathwayiUPA00333; UER00453.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan 2,3-dioxygenaseUniRule annotation (EC:1.13.11.11UniRule annotation)
Short name:
TDOUniRule annotation
Alternative name(s):
Tryptamin 2,3-dioxygenaseUniRule annotation
Tryptophan oxygenaseUniRule annotation
Short name:
TOUniRule annotation
Short name:
TRPOUniRule annotation
Tryptophan pyrrolaseUniRule annotation
TryptophanaseUniRule annotation
Gene namesi
Name:Tdo2
Synonyms:Tdo
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi68370. Tdo2.

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2686.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000724011 – 406Tryptophan 2,3-dioxygenaseAdd BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP21643.
PRIDEiP21643.

PTM databases

iPTMnetiP21643.
PhosphoSitePlusiP21643.

Expressioni

Tissue specificityi

Liver.

Inductioni

By dexamethasone.1 Publication

Gene expression databases

BgeeiENSRNOG00000011612.
GenevisibleiP21643. RN.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015732.

Chemistry databases

BindingDBiP21643.

Structurei

3D structure databases

ProteinModelPortaliP21643.
SMRiP21643.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni42 – 46Substrate bindingUniRule annotation5
Regioni72 – 76Substrate bindingUniRule annotation5

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili229 – 268UniRule annotationAdd BLAST40

Sequence similaritiesi

Belongs to the tryptophan 2,3-dioxygenase family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3906. Eukaryota.
COG3483. LUCA.
GeneTreeiENSGT00390000008593.
HOGENOMiHOG000221584.
HOVERGENiHBG003043.
InParanoidiP21643.
KOiK00453.
OMAiWRYNHVL.
OrthoDBiEOG091G080K.
PhylomeDBiP21643.
TreeFamiTF105827.

Family and domain databases

HAMAPiMF_01972. T23O. 1 hit.
InterProiIPR004981. Trp_2_3_dOase.
[Graphical view]
PANTHERiPTHR10138. PTHR10138. 1 hit.
PfamiPF03301. Trp_dioxygenase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21643-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGCPFSGNS VGYTLKNLSM EDNEEDGAQT GVNRASKGGL IYGDYLQLEK
60 70 80 90 100
ILNAQELQSE IKGNKIHDEH LFIITHQAYE LWFKQILWEL DSVREIFQNG
110 120 130 140 150
HVRDERNMLK VMTRMHRVVV IFKLLVQQFS VLETMTALDF NDFREYLSPA
160 170 180 190 200
SGFQSLQFRL LENKIGVLQS LRVPYNRKHY RDNFEGDYNE LLLKSEQEQT
210 220 230 240 250
LLQLVEAWLE RTPGLEPHGF NFWGKFEKNI LKGLEEEFLK IQAKKDSEEK
260 270 280 290 300
EEQMAEFRKQ KEVLLCLFDE KRHDYLLSKG ERRLSYRALQ GALMIYFYRE
310 320 330 340 350
EPRFQVPFQL LTSLMDIDTL MTKWRYNHVC MVHRMLGSKA GTGGSSGYYY
360 370 380 390 400
LRSTVSDRYK VFVDLFNLSS YLVPRHWIPK MNPIIHKFLY TAEYSDSSYF

SSDESD
Length:406
Mass (Da):47,857
Last modified:May 1, 1991 - v1
Checksum:i9C45973BEE3BF93A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti207Missing in X60833 (PubMed:1511007).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55167 mRNA. Translation: AAA63503.1.
BC089802 mRNA. Translation: AAH89802.1.
X05145 Genomic DNA. Translation: CAA28794.1.
X01849 Genomic DNA. Translation: CAA25974.1.
X60833 Genomic DNA. No translation available.
PIRiA35484.
RefSeqiNP_071798.1. NM_022403.2.
UniGeneiRn.1029.

Genome annotation databases

EnsembliENSRNOT00000015732; ENSRNOP00000015732; ENSRNOG00000011612.
GeneIDi64206.
KEGGirno:64206.
UCSCiRGD:68370. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55167 mRNA. Translation: AAA63503.1.
BC089802 mRNA. Translation: AAH89802.1.
X05145 Genomic DNA. Translation: CAA28794.1.
X01849 Genomic DNA. Translation: CAA25974.1.
X60833 Genomic DNA. No translation available.
PIRiA35484.
RefSeqiNP_071798.1. NM_022403.2.
UniGeneiRn.1029.

3D structure databases

ProteinModelPortaliP21643.
SMRiP21643.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015732.

Chemistry databases

BindingDBiP21643.
ChEMBLiCHEMBL2686.

PTM databases

iPTMnetiP21643.
PhosphoSitePlusiP21643.

Proteomic databases

PaxDbiP21643.
PRIDEiP21643.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015732; ENSRNOP00000015732; ENSRNOG00000011612.
GeneIDi64206.
KEGGirno:64206.
UCSCiRGD:68370. rat.

Organism-specific databases

CTDi6999.
RGDi68370. Tdo2.

Phylogenomic databases

eggNOGiKOG3906. Eukaryota.
COG3483. LUCA.
GeneTreeiENSGT00390000008593.
HOGENOMiHOG000221584.
HOVERGENiHBG003043.
InParanoidiP21643.
KOiK00453.
OMAiWRYNHVL.
OrthoDBiEOG091G080K.
PhylomeDBiP21643.
TreeFamiTF105827.

Enzyme and pathway databases

UniPathwayiUPA00333; UER00453.
BRENDAi1.13.11.11. 5301.
ReactomeiR-RNO-71240. Tryptophan catabolism.

Miscellaneous databases

PROiP21643.

Gene expression databases

BgeeiENSRNOG00000011612.
GenevisibleiP21643. RN.

Family and domain databases

HAMAPiMF_01972. T23O. 1 hit.
InterProiIPR004981. Trp_2_3_dOase.
[Graphical view]
PANTHERiPTHR10138. PTHR10138. 1 hit.
PfamiPF03301. Trp_dioxygenase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiT23O_RAT
AccessioniPrimary (citable) accession number: P21643
Secondary accession number(s): Q5EBC2, Q6LBW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 30, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.