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P21642 (PCKGM_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
Short name=PEPCK-M
EC=4.1.1.32
Alternative name(s):
Phosphoenolpyruvate carboxylase
Gene names
Name:PCK2
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length641 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Facilitates the recycling of lactate carbon in the liver.

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactor

Binds 1 manganese ion per subunit.

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

Subunit structure

Monomer.

Subcellular location

Mitochondrion.

Tissue specificity

Present in liver and kidney. Ref.1

Induction

Appears to be constitutively expressed.

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. In birds, PEPCK-M is the sole form of hepatic PEPCK, but it constitutes 60% of the enzyme in the kidney.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3333Mitochondrion
Chain34 – 641608Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
PRO_0000023570

Regions

Nucleotide binding306 – 3116GTP
Nucleotide binding549 – 5524GTP By similarity
Region422 – 4243Substrate binding

Sites

Active site3071
Metal binding2631Manganese
Metal binding2831Manganese
Metal binding3301Manganese
Binding site1051Substrate
Binding site2561Substrate; via amide nitrogen
Binding site2631Substrate By similarity
Binding site3051Substrate By similarity
Binding site4241GTP By similarity
Binding site4551GTP

Experimental info

Sequence conflict1071E → Q in AAA49006. Ref.1
Sequence conflict129 – 1302GP → S in AAA49006. Ref.1
Sequence conflict2351P → R in AAA49006. Ref.1
Sequence conflict2681R → A in AAA49006. Ref.1
Sequence conflict3391E → R in AAA49006. Ref.1
Sequence conflict5021R → S in AAA49006. Ref.1

Secondary structure

1 ........................................................................................................................... 641
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P21642 [UniParc].

Last modified March 24, 2009. Version 2.
Checksum: A5C2BA63C659E633

FASTA64171,106
        10         20         30         40         50         60 
MFWLRGGAQS CRGGETEDRM QRGMWGVGLA RRRLSTSLSA LPAAARDFVE EAVRLCRPRE 

        70         80         90        100        110        120 
VLLCDGSEEE GKELLRGLQD DGVLHPLPKY DNCWLARTDP RDVARVESKT VLVTPEQSDA 

       130        140        150        160        170        180 
VPPPPPSGGP PQLGNWMSPN AFQAAVQERF PGCMAGRPLY VIPFSMGPPT SPLAKLGVQV 

       190        200        210        220        230        240 
TDSPYVVLSM RIMTRVGPAV LQRLDDDFVR CLHSVGRPLP LTEPLVSSWP CDRSPVLVAH 

       250        260        270        280        290        300 
IPSERRIVSF GSGYGGNSLL GKKCFALRIA SRMAQQQGWL AEHMLILGVT SPSGEKRYMA 

       310        320        330        340        350        360 
AAFPSACGKT NLAMMTPSLP GWRIHCVGDD IAWMKFDDEG RLRAINPERG FFGVAPGTSS 

       370        380        390        400        410        420 
RTNPNAMATI ARNTIFTNVG LRSDGGVYWD GLDEPTEPGV TYTSWLGKPW KHGDPEPCAH 

       430        440        450        460        470        480 
PNSRFCAPAD QCPIMDPRWD DPEGVPIDAI IFGGRRPRGV PLVVEAFGWR HGVFMGSAMR 

       490        500        510        520        530        540 
SEATAAAEHK GGRLMHDPFA MRPFFGYNAG RYLEHWLSTG LRSNARLPRL FHVNWFLRDN 

       550        560        570        580        590        600 
EGRFVWPGFG HNARVLAWIF GRIQGRDTAR PTPIGWVPKE GDLDLGGLPG VDYSQLFPME 

       610        620        630        640 
KGFWEEECRQ LREYYGENFG ADLPRDVMAE LEGLEERVRK M

« Hide

References

[1]"Mitochondrial phosphoenolpyruvate carboxykinase from the chicken. Comparison of the cDNA and protein sequences with the cytosolic isozyme."
Weldon S.L., Rando A., Matathias A.S., Hod Y., Kalonick P.A., Savon S., Cook J.S., Hanson R.W.
J. Biol. Chem. 265:7308-7317(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, TISSUE SPECIFICITY.
Tissue: Liver.
[2]"Structural insights into the mechanism of PEPCK catalysis."
Holyoak T., Sullivan S.M., Nowak T.
Biochemistry 45:8254-8263(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 34-641 IN COMPLEX WITH MANGANESE; GTP AND SUBSTRATE, SEQUENCE REVISION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		J05419 mRNA. Translation: AAA49006.1.
IPIIPI00583818.
PIRQYCHGM. A35191.
RefSeqNP_990801.1. NM_205470.1.
UniGeneGga.979.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2FAFX-ray1.70A/B34-641[»]
2FAHX-ray2.09A/B/C/D34-641[»]
2QZYX-ray1.90A/B34-641[»]
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID396457.
KEGGgga:396457.

Organism-specific databases

CTD5106.

Phylogenomic databases

HOVERGENHBG053651.
KOK01596.

Enzyme and pathway databases

ReactomeREACT_115655. Metabolism.
SABIO-RKP21642.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. PEP_carboxykinase_N. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP21642.
NextBio20816498.

Entry information

Entry namePCKGM_CHICK
AccessionPrimary (citable) accession number: P21642
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: March 24, 2009
Last modified: April 3, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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