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P21642

- PCKGM_CHICK

UniProt

P21642 - PCKGM_CHICK

Protein

Phosphoenolpyruvate carboxykinase [GTP], mitochondrial

Gene

PCK2

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 2 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Facilitates the recycling of lactate carbon in the liver.

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

    Cofactori

    Binds 1 manganese ion per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei105 – 1051Substrate1 Publication
    Binding sitei256 – 2561Substrate; via amide nitrogen1 Publication
    Metal bindingi263 – 2631Manganese1 Publication
    Binding sitei263 – 2631SubstrateBy similarity
    Metal bindingi283 – 2831Manganese; via tele nitrogen1 Publication
    Binding sitei305 – 3051SubstrateBy similarity
    Active sitei307 – 3071
    Metal bindingi330 – 3301Manganese1 Publication
    Binding sitei424 – 4241GTPBy similarity
    Binding sitei455 – 4551GTP1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi306 – 3116GTP1 Publication
    Nucleotide bindingi549 – 5524GTPBy similarity

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: Reactome
    2. gluconeogenesis Source: Reactome
    3. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_115534. Gluconeogenesis.
    SABIO-RKP21642.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (EC:4.1.1.32)
    Short name:
    PEPCK-M
    Gene namesi
    Name:PCK2
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: Reactome

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3333MitochondrionAdd
    BLAST
    Chaini34 – 641608Phosphoenolpyruvate carboxykinase [GTP], mitochondrialPRO_0000023570Add
    BLAST

    Expressioni

    Tissue specificityi

    Present in liver and kidney.1 Publication

    Inductioni

    Appears to be constitutively expressed.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Structurei

    Secondary structure

    1
    641
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi43 – 5614
    Beta strandi59 – 635
    Helixi68 – 8013
    Beta strandi83 – 864
    Beta strandi90 – 923
    Beta strandi94 – 963
    Beta strandi102 – 1043
    Helixi107 – 1093
    Beta strandi110 – 1134
    Helixi117 – 1193
    Beta strandi121 – 1233
    Helixi139 – 14911
    Turni151 – 1566
    Beta strandi157 – 16711
    Beta strandi171 – 1733
    Beta strandi175 – 1828
    Helixi184 – 19310
    Beta strandi194 – 1974
    Helixi198 – 2014
    Beta strandi209 – 2146
    Helixi233 – 2353
    Beta strandi237 – 2415
    Helixi242 – 2443
    Beta strandi246 – 2516
    Helixi255 – 2584
    Helixi261 – 2677
    Helixi268 – 27710
    Beta strandi280 – 2834
    Beta strandi285 – 2906
    Beta strandi296 – 3027
    Helixi312 – 3143
    Beta strandi323 – 3308
    Beta strandi332 – 3365
    Beta strandi340 – 3456
    Beta strandi349 – 3546
    Turni360 – 3623
    Helixi364 – 3696
    Beta strandi371 – 3733
    Beta strandi375 – 3784
    Beta strandi380 – 3823
    Beta strandi407 – 4104
    Beta strandi424 – 4285
    Helixi429 – 4313
    Turni437 – 4404
    Beta strandi445 – 45410
    Beta strandi457 – 4604
    Beta strandi462 – 4654
    Helixi469 – 4779
    Beta strandi480 – 4823
    Beta strandi487 – 4893
    Beta strandi494 – 4963
    Helixi498 – 5003
    Helixi502 – 5043
    Helixi509 – 51911
    Beta strandi529 – 5357
    Beta strandi542 – 5465
    Helixi549 – 5513
    Helixi552 – 56312
    Beta strandi569 – 5724
    Beta strandi575 – 5784
    Turni580 – 5823
    Helixi593 – 5964
    Helixi601 – 61818
    Helixi620 – 6223
    Helixi625 – 63915

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FAFX-ray1.70A/B34-641[»]
    2FAHX-ray2.09A/B/C/D34-641[»]
    2QZYX-ray1.90A/B34-641[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP21642.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni422 – 4243Substrate binding

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOVERGENiHBG053651.
    KOiK01596.
    PhylomeDBiP21642.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P21642-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFWLRGGAQS CRGGETEDRM QRGMWGVGLA RRRLSTSLSA LPAAARDFVE    50
    EAVRLCRPRE VLLCDGSEEE GKELLRGLQD DGVLHPLPKY DNCWLARTDP 100
    RDVARVESKT VLVTPEQSDA VPPPPPSGGP PQLGNWMSPN AFQAAVQERF 150
    PGCMAGRPLY VIPFSMGPPT SPLAKLGVQV TDSPYVVLSM RIMTRVGPAV 200
    LQRLDDDFVR CLHSVGRPLP LTEPLVSSWP CDRSPVLVAH IPSERRIVSF 250
    GSGYGGNSLL GKKCFALRIA SRMAQQQGWL AEHMLILGVT SPSGEKRYMA 300
    AAFPSACGKT NLAMMTPSLP GWRIHCVGDD IAWMKFDDEG RLRAINPERG 350
    FFGVAPGTSS RTNPNAMATI ARNTIFTNVG LRSDGGVYWD GLDEPTEPGV 400
    TYTSWLGKPW KHGDPEPCAH PNSRFCAPAD QCPIMDPRWD DPEGVPIDAI 450
    IFGGRRPRGV PLVVEAFGWR HGVFMGSAMR SEATAAAEHK GGRLMHDPFA 500
    MRPFFGYNAG RYLEHWLSTG LRSNARLPRL FHVNWFLRDN EGRFVWPGFG 550
    HNARVLAWIF GRIQGRDTAR PTPIGWVPKE GDLDLGGLPG VDYSQLFPME 600
    KGFWEEECRQ LREYYGENFG ADLPRDVMAE LEGLEERVRK M 641
    Length:641
    Mass (Da):71,106
    Last modified:March 24, 2009 - v2
    Checksum:iA5C2BA63C659E633
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti107 – 1071E → Q in AAA49006. (PubMed:2110163)Curated
    Sequence conflicti129 – 1302GP → S in AAA49006. (PubMed:2110163)Curated
    Sequence conflicti235 – 2351P → R in AAA49006. (PubMed:2110163)Curated
    Sequence conflicti268 – 2681R → A in AAA49006. (PubMed:2110163)Curated
    Sequence conflicti339 – 3391E → R in AAA49006. (PubMed:2110163)Curated
    Sequence conflicti502 – 5021R → S in AAA49006. (PubMed:2110163)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05419 mRNA. Translation: AAA49006.1.
    PIRiA35191. QYCHGM.
    RefSeqiNP_990801.1. NM_205470.1.
    UniGeneiGga.979.

    Genome annotation databases

    GeneIDi396457.
    KEGGigga:396457.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05419 mRNA. Translation: AAA49006.1 .
    PIRi A35191. QYCHGM.
    RefSeqi NP_990801.1. NM_205470.1.
    UniGenei Gga.979.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FAF X-ray 1.70 A/B 34-641 [» ]
    2FAH X-ray 2.09 A/B/C/D 34-641 [» ]
    2QZY X-ray 1.90 A/B 34-641 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 396457.
    KEGGi gga:396457.

    Organism-specific databases

    CTDi 5106.

    Phylogenomic databases

    HOVERGENi HBG053651.
    KOi K01596.
    PhylomeDBi P21642.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    Reactomei REACT_115534. Gluconeogenesis.
    SABIO-RK P21642.

    Miscellaneous databases

    EvolutionaryTracei P21642.
    NextBioi 20816498.
    PROi P21642.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mitochondrial phosphoenolpyruvate carboxykinase from the chicken. Comparison of the cDNA and protein sequences with the cytosolic isozyme."
      Weldon S.L., Rando A., Matathias A.S., Hod Y., Kalonick P.A., Savon S., Cook J.S., Hanson R.W.
      J. Biol. Chem. 265:7308-7317(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, TISSUE SPECIFICITY.
      Tissue: Liver.
    2. "Structural insights into the mechanism of PEPCK catalysis."
      Holyoak T., Sullivan S.M., Nowak T.
      Biochemistry 45:8254-8263(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 34-641 IN COMPLEX WITH MANGANESE; GTP AND SUBSTRATE, SEQUENCE REVISION.

    Entry informationi

    Entry nameiPCKGM_CHICK
    AccessioniPrimary (citable) accession number: P21642
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 106 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. In birds, PEPCK-M is the sole form of hepatic PEPCK, but it constitutes 60% of the enzyme in the kidney.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3