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P21642

- PCKGM_CHICK

UniProt

P21642 - PCKGM_CHICK

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Protein

Phosphoenolpyruvate carboxykinase [GTP], mitochondrial

Gene

PCK2

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Facilitates the recycling of lactate carbon in the liver.

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactori

Mn2+Note: Binds 1 Mn(2+) ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei105 – 1051Substrate1 Publication
Binding sitei256 – 2561Substrate; via amide nitrogen1 Publication
Metal bindingi263 – 2631Manganese1 Publication
Binding sitei263 – 2631SubstrateBy similarity
Metal bindingi283 – 2831Manganese; via tele nitrogen1 Publication
Binding sitei305 – 3051SubstrateBy similarity
Active sitei307 – 3071
Metal bindingi330 – 3301Manganese1 Publication
Binding sitei424 – 4241GTPBy similarity
Binding sitei455 – 4551GTP1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi306 – 3116GTP1 Publication
Nucleotide bindingi549 – 5524GTPBy similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: Reactome
  2. gluconeogenesis Source: Reactome
  3. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_115534. Gluconeogenesis.
SABIO-RKP21642.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (EC:4.1.1.32)
Short name:
PEPCK-M
Gene namesi
Name:PCK2
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333MitochondrionAdd
BLAST
Chaini34 – 641608Phosphoenolpyruvate carboxykinase [GTP], mitochondrialPRO_0000023570Add
BLAST

Expressioni

Tissue specificityi

Present in liver and kidney.1 Publication

Inductioni

Appears to be constitutively expressed.

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

1
641
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi43 – 5614Combined sources
Beta strandi59 – 635Combined sources
Helixi68 – 8013Combined sources
Beta strandi83 – 864Combined sources
Beta strandi90 – 923Combined sources
Beta strandi94 – 963Combined sources
Beta strandi102 – 1043Combined sources
Helixi107 – 1093Combined sources
Beta strandi110 – 1134Combined sources
Helixi117 – 1193Combined sources
Beta strandi121 – 1233Combined sources
Helixi139 – 14911Combined sources
Turni151 – 1566Combined sources
Beta strandi157 – 16711Combined sources
Beta strandi171 – 1733Combined sources
Beta strandi175 – 1828Combined sources
Helixi184 – 19310Combined sources
Beta strandi194 – 1974Combined sources
Helixi198 – 2014Combined sources
Beta strandi209 – 2146Combined sources
Helixi233 – 2353Combined sources
Beta strandi237 – 2415Combined sources
Helixi242 – 2443Combined sources
Beta strandi246 – 2516Combined sources
Helixi255 – 2584Combined sources
Helixi261 – 2677Combined sources
Helixi268 – 27710Combined sources
Beta strandi280 – 2834Combined sources
Beta strandi285 – 2906Combined sources
Beta strandi296 – 3027Combined sources
Helixi312 – 3143Combined sources
Beta strandi323 – 3308Combined sources
Beta strandi332 – 3365Combined sources
Beta strandi340 – 3456Combined sources
Beta strandi349 – 3546Combined sources
Turni360 – 3623Combined sources
Helixi364 – 3696Combined sources
Beta strandi371 – 3733Combined sources
Beta strandi375 – 3784Combined sources
Beta strandi380 – 3823Combined sources
Beta strandi407 – 4104Combined sources
Beta strandi424 – 4285Combined sources
Helixi429 – 4313Combined sources
Turni437 – 4404Combined sources
Beta strandi445 – 45410Combined sources
Beta strandi457 – 4604Combined sources
Beta strandi462 – 4654Combined sources
Helixi469 – 4779Combined sources
Beta strandi480 – 4823Combined sources
Beta strandi487 – 4893Combined sources
Beta strandi494 – 4963Combined sources
Helixi498 – 5003Combined sources
Helixi502 – 5043Combined sources
Helixi509 – 51911Combined sources
Beta strandi529 – 5357Combined sources
Beta strandi542 – 5465Combined sources
Helixi549 – 5513Combined sources
Helixi552 – 56312Combined sources
Beta strandi569 – 5724Combined sources
Beta strandi575 – 5784Combined sources
Turni580 – 5823Combined sources
Helixi593 – 5964Combined sources
Helixi601 – 61818Combined sources
Helixi620 – 6223Combined sources
Helixi625 – 63915Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FAFX-ray1.70A/B34-641[»]
2FAHX-ray2.09A/B/C/D34-641[»]
2QZYX-ray1.90A/B34-641[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21642.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni422 – 4243Substrate binding

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOVERGENiHBG053651.
InParanoidiP21642.
KOiK01596.
PhylomeDBiP21642.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21642-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFWLRGGAQS CRGGETEDRM QRGMWGVGLA RRRLSTSLSA LPAAARDFVE
60 70 80 90 100
EAVRLCRPRE VLLCDGSEEE GKELLRGLQD DGVLHPLPKY DNCWLARTDP
110 120 130 140 150
RDVARVESKT VLVTPEQSDA VPPPPPSGGP PQLGNWMSPN AFQAAVQERF
160 170 180 190 200
PGCMAGRPLY VIPFSMGPPT SPLAKLGVQV TDSPYVVLSM RIMTRVGPAV
210 220 230 240 250
LQRLDDDFVR CLHSVGRPLP LTEPLVSSWP CDRSPVLVAH IPSERRIVSF
260 270 280 290 300
GSGYGGNSLL GKKCFALRIA SRMAQQQGWL AEHMLILGVT SPSGEKRYMA
310 320 330 340 350
AAFPSACGKT NLAMMTPSLP GWRIHCVGDD IAWMKFDDEG RLRAINPERG
360 370 380 390 400
FFGVAPGTSS RTNPNAMATI ARNTIFTNVG LRSDGGVYWD GLDEPTEPGV
410 420 430 440 450
TYTSWLGKPW KHGDPEPCAH PNSRFCAPAD QCPIMDPRWD DPEGVPIDAI
460 470 480 490 500
IFGGRRPRGV PLVVEAFGWR HGVFMGSAMR SEATAAAEHK GGRLMHDPFA
510 520 530 540 550
MRPFFGYNAG RYLEHWLSTG LRSNARLPRL FHVNWFLRDN EGRFVWPGFG
560 570 580 590 600
HNARVLAWIF GRIQGRDTAR PTPIGWVPKE GDLDLGGLPG VDYSQLFPME
610 620 630 640
KGFWEEECRQ LREYYGENFG ADLPRDVMAE LEGLEERVRK M
Length:641
Mass (Da):71,106
Last modified:March 24, 2009 - v2
Checksum:iA5C2BA63C659E633
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti107 – 1071E → Q in AAA49006. (PubMed:2110163)Curated
Sequence conflicti129 – 1302GP → S in AAA49006. (PubMed:2110163)Curated
Sequence conflicti235 – 2351P → R in AAA49006. (PubMed:2110163)Curated
Sequence conflicti268 – 2681R → A in AAA49006. (PubMed:2110163)Curated
Sequence conflicti339 – 3391E → R in AAA49006. (PubMed:2110163)Curated
Sequence conflicti502 – 5021R → S in AAA49006. (PubMed:2110163)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05419 mRNA. Translation: AAA49006.1.
PIRiA35191. QYCHGM.
RefSeqiNP_990801.1. NM_205470.1.
UniGeneiGga.979.

Genome annotation databases

GeneIDi396457.
KEGGigga:396457.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05419 mRNA. Translation: AAA49006.1 .
PIRi A35191. QYCHGM.
RefSeqi NP_990801.1. NM_205470.1.
UniGenei Gga.979.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2FAF X-ray 1.70 A/B 34-641 [» ]
2FAH X-ray 2.09 A/B/C/D 34-641 [» ]
2QZY X-ray 1.90 A/B 34-641 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 396457.
KEGGi gga:396457.

Organism-specific databases

CTDi 5106.

Phylogenomic databases

HOVERGENi HBG053651.
InParanoidi P21642.
KOi K01596.
PhylomeDBi P21642.

Enzyme and pathway databases

UniPathwayi UPA00138 .
Reactomei REACT_115534. Gluconeogenesis.
SABIO-RK P21642.

Miscellaneous databases

EvolutionaryTracei P21642.
NextBioi 20816498.
PROi P21642.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Mitochondrial phosphoenolpyruvate carboxykinase from the chicken. Comparison of the cDNA and protein sequences with the cytosolic isozyme."
    Weldon S.L., Rando A., Matathias A.S., Hod Y., Kalonick P.A., Savon S., Cook J.S., Hanson R.W.
    J. Biol. Chem. 265:7308-7317(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, TISSUE SPECIFICITY.
    Tissue: Liver.
  2. "Structural insights into the mechanism of PEPCK catalysis."
    Holyoak T., Sullivan S.M., Nowak T.
    Biochemistry 45:8254-8263(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 34-641 IN COMPLEX WITH MANGANESE; GTP AND SUBSTRATE, SEQUENCE REVISION.

Entry informationi

Entry nameiPCKGM_CHICK
AccessioniPrimary (citable) accession number: P21642
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: March 24, 2009
Last modified: November 26, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. In birds, PEPCK-M is the sole form of hepatic PEPCK, but it constitutes 60% of the enzyme in the kidney.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3