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Protein

Precorrin-8X methylmutase

Gene

cobH

Organism
Pseudomonas denitrificans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of precorrin-8X to hydrogenobyrinate.1 Publication

Catalytic activityi

Precorrin-8X = hydrogenobyrinate.1 Publication

Kineticsi

  1. KM=0.91 µM for precorrin-8X1 Publication
  1. Vmax=230 nmol/h/mg enzyme1 Publication

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 8 of the subpathway that synthesizes cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route).
Proteins known to be involved in the 10 steps of the subpathway in this organism are:
  1. Precorrin-2 C(20)-methyltransferase (cobI)
  2. Precorrin-3B synthase (cobG)
  3. Precorrin-3B C(17)-methyltransferase (cobJ)
  4. Precorrin-4 C(11)-methyltransferase (cobM)
  5. Precorrin-6A synthase [deacetylating] (cobF)
  6. Precorrin-6A reductase (cobK)
  7. Precorrin-6Y C(5,15)-methyltransferase [decarboxylating] (cobL)
  8. Precorrin-8X methylmutase (cobH)
  9. Hydrogenobyrinate a,c-diamide synthase (cobB)
  10. Aerobic cobaltochelatase subunit CobN (cobN), Aerobic cobaltochelatase subunit CobT (cobT), Aerobic cobaltochelatase subunit CobS (cobS)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route), the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei17Substrate1
Binding sitei40Substrate1
Active sitei43Proton donor/acceptor1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processCobalamin biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-115
BRENDAi5.4.99.61 5114
SABIO-RKP21638
UniPathwayiUPA00148; UER00219

Names & Taxonomyi

Protein namesi
Recommended name:
Precorrin-8X methylmutase (EC:5.4.99.61)
Alternative name(s):
HBA synthase
Precorrin isomerase
Gene namesi
Name:cobH
OrganismiPseudomonas denitrificans
Taxonomic identifieri43306 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Pathology & Biotechi

Chemistry databases

DrugBankiDB02460 Hydrogenobyrinic Acid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001359262 – 210Precorrin-8X methylmutaseAdd BLAST209

Proteomic databases

PRIDEiP21638

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1210
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 24Combined sources15
Beta strandi28 – 30Combined sources3
Helixi32 – 45Combined sources14
Helixi48 – 53Combined sources6
Beta strandi54 – 56Combined sources3
Helixi60 – 69Combined sources10
Beta strandi74 – 78Combined sources5
Helixi79 – 84Combined sources6
Helixi87 – 89Combined sources3
Beta strandi96 – 98Combined sources3
Helixi104 – 112Combined sources9
Helixi116 – 119Combined sources4
Helixi120 – 124Combined sources5
Helixi125 – 128Combined sources4
Beta strandi132 – 135Combined sources4
Helixi139 – 150Combined sources12
Beta strandi157 – 161Combined sources5
Beta strandi165 – 168Combined sources4
Helixi169 – 178Combined sources10
Beta strandi185 – 188Combined sources4
Helixi195 – 206Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F2VX-ray2.10A2-210[»]
1I1HX-ray2.60A2-210[»]
ProteinModelPortaliP21638
SMRiP21638
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21638

Family & Domainsi

Sequence similaritiesi

Belongs to the CobH/CbiC family.Curated

Phylogenomic databases

KOiK06042

Family and domain databases

Gene3Di3.40.50.10230, 1 hit
InterProiView protein in InterPro
IPR003722 Cbl_synth_CobH/CbiC
IPR036588 CobH/CbiC_sf
PfamiView protein in Pfam
PF02570 CbiC, 1 hit
SUPFAMiSSF63965 SSF63965, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21638-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEYDYIRDG NAIYERSFAI IRAEADLSRF SEEEADLAVR MVHACGSVEA
60 70 80 90 100
TRQFVFSPDF VSSARAALKA GAPILCDAEM VAHGVTRARL PAGNEVICTL
110 120 130 140 150
RDPRTPALAA EIGNTRSAAA LKLWSERLAG SVVAIGNAPT ALFFLLEMLR
160 170 180 190 200
DGAPKPAAIL GMPVGFVGAA ESKDALAENS YGVPFAIVRG RLGGSAMTAA
210
ALNSLARPGL
Length:210
Mass (Da):22,064
Last modified:May 1, 1991 - v1
Checksum:i506D1C4C5D972B3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59301 Genomic DNA Translation: AAA25796.1

Genome annotation databases

KEGGiag:AAA25796

Similar proteinsi

Entry informationi

Entry nameiCOBH_PSEDE
AccessioniPrimary (citable) accession number: P21638
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: March 28, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

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