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P21638 (COBH_PSEDE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Precorrin-8X methylmutase

EC=5.4.99.61
Alternative name(s):
HBA synthase
Precorrin isomerase
Gene names
Name:cobH
OrganismPseudomonas denitrificans
Taxonomic identifier43306 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length210 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the conversion of precorrin-8X to hydrogenobyrinic acid; a methyl migration reaction during the transformation of precorrin-3 to form cobyrinic acid.

Catalytic activity

Precorrin-8X = hydrogenobyrinate.

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 8/10.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the CobH family.

Ontologies

Keywords
   Biological processCobalamin biosynthesis
   Molecular functionIsomerase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processcobalamin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionprecorrin-8X methylmutase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 210210Precorrin-8X methylmutase
PRO_0000135926

Sites

Active site431

Secondary structure

...................................... 210
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P21638 [UniParc].

Last modified May 1, 1991. Version 1.
Checksum: 506D1C4C5D972B3F

FASTA21022,064
        10         20         30         40         50         60 
MPEYDYIRDG NAIYERSFAI IRAEADLSRF SEEEADLAVR MVHACGSVEA TRQFVFSPDF 

        70         80         90        100        110        120 
VSSARAALKA GAPILCDAEM VAHGVTRARL PAGNEVICTL RDPRTPALAA EIGNTRSAAA 

       130        140        150        160        170        180 
LKLWSERLAG SVVAIGNAPT ALFFLLEMLR DGAPKPAAIL GMPVGFVGAA ESKDALAENS 

       190        200        210 
YGVPFAIVRG RLGGSAMTAA ALNSLARPGL 

« Hide

References

[1]"Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans fragment carrying eight genes involved in transformation of precorrin-2 to cobyrinic acid."
Crouzet J., Cameron B., Cauchois L., Rigault S., Rouyez M.-C., Blanche F., Thibaut D., Debussche L.
J. Bacteriol. 172:5980-5990(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SC510.
[2]"The final step in the biosynthesis of hydrogenobyrinic acid is catalyzed by the cobH gene product with precorrin-8x as the substrate."
Thibaut D., Couder M., Famechon A., Debussche L., Cameron B., Crouzet J., Blanche F.
J. Bacteriol. 174:1043-1049(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[3]"Crystal structure of precorrin-8x methyl mutase."
Shipman L.W., Li D., Roessner C.A., Scott A.I., Sacchettini J.C.
Structure 9:587-596(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 6-210.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M59301 Genomic DNA. Translation: AAA25796.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1F2VX-ray2.10A2-210[»]
1I1HX-ray2.60A2-210[»]
ProteinModelPortalP21638.
SMRP21638. Positions 2-210.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-115.
UniPathwayUPA00148; UER00219.

Family and domain databases

Gene3D3.40.50.10230. 1 hit.
InterProIPR003722. Cbl_synth_CobH/CbiC_core.
[Graphical view]
PfamPF02570. CbiC. 1 hit.
[Graphical view]
SUPFAMSSF63965. SSF63965. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP21638.

Entry information

Entry nameCOBH_PSEDE
AccessionPrimary (citable) accession number: P21638
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: April 16, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways