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P21638

- COBH_PSEDE

UniProt

P21638 - COBH_PSEDE

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Protein

Precorrin-8X methylmutase

Gene

cobH

Organism
Pseudomonas denitrificans
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of precorrin-8X to hydrogenobyrinate.1 Publication

Catalytic activityi

Precorrin-8X = hydrogenobyrinate.1 Publication

Kineticsi

  1. KM=0.91 µM for precorrin-8X1 Publication

Vmax=230 nmol/h/mg enzyme1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei17 – 171Substrate
Binding sitei40 – 401Substrate
Active sitei43 – 431Proton donor/acceptor1 Publication

GO - Molecular functioni

  1. precorrin-8X methylmutase activity Source: InterPro

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Cobalamin biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-115.
RETL1328306-WGS:GSTH-6323-MONOMER.
UniPathwayiUPA00148; UER00219.

Names & Taxonomyi

Protein namesi
Recommended name:
Precorrin-8X methylmutase (EC:5.4.99.61)
Alternative name(s):
HBA synthase
Precorrin isomerase
Gene namesi
Name:cobH
OrganismiPseudomonas denitrificans
Taxonomic identifieri43306 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 210209Precorrin-8X methylmutasePRO_0000135926Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1
210
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 2415
Beta strandi28 – 303
Helixi32 – 4514
Helixi48 – 536
Beta strandi54 – 563
Helixi60 – 6910
Beta strandi74 – 785
Helixi79 – 846
Helixi87 – 893
Beta strandi96 – 983
Helixi104 – 1129
Helixi116 – 1194
Helixi120 – 1245
Helixi125 – 1284
Beta strandi132 – 1354
Helixi139 – 15012
Beta strandi157 – 1615
Beta strandi165 – 1684
Helixi169 – 17810
Beta strandi185 – 1884
Helixi195 – 20612

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F2VX-ray2.10A2-210[»]
1I1HX-ray2.60A2-210[»]
ProteinModelPortaliP21638.
SMRiP21638. Positions 2-210.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21638.

Family & Domainsi

Sequence similaritiesi

Belongs to the CobH/CbiC family.Curated

Family and domain databases

Gene3Di3.40.50.10230. 1 hit.
InterProiIPR003722. Cbl_synth_CobH/CbiC.
[Graphical view]
PfamiPF02570. CbiC. 1 hit.
[Graphical view]
SUPFAMiSSF63965. SSF63965. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21638-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPEYDYIRDG NAIYERSFAI IRAEADLSRF SEEEADLAVR MVHACGSVEA
60 70 80 90 100
TRQFVFSPDF VSSARAALKA GAPILCDAEM VAHGVTRARL PAGNEVICTL
110 120 130 140 150
RDPRTPALAA EIGNTRSAAA LKLWSERLAG SVVAIGNAPT ALFFLLEMLR
160 170 180 190 200
DGAPKPAAIL GMPVGFVGAA ESKDALAENS YGVPFAIVRG RLGGSAMTAA
210
ALNSLARPGL
Length:210
Mass (Da):22,064
Last modified:May 1, 1991 - v1
Checksum:i506D1C4C5D972B3F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59301 Genomic DNA. Translation: AAA25796.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59301 Genomic DNA. Translation: AAA25796.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1F2V X-ray 2.10 A 2-210 [» ]
1I1H X-ray 2.60 A 2-210 [» ]
ProteinModelPortali P21638.
SMRi P21638. Positions 2-210.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00219 .
BioCyci MetaCyc:MONOMER-115.
RETL1328306-WGS:GSTH-6323-MONOMER.

Miscellaneous databases

EvolutionaryTracei P21638.

Family and domain databases

Gene3Di 3.40.50.10230. 1 hit.
InterProi IPR003722. Cbl_synth_CobH/CbiC.
[Graphical view ]
Pfami PF02570. CbiC. 1 hit.
[Graphical view ]
SUPFAMi SSF63965. SSF63965. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans fragment carrying eight genes involved in transformation of precorrin-2 to cobyrinic acid."
    Crouzet J., Cameron B., Cauchois L., Rigault S., Rouyez M.-C., Blanche F., Thibaut D., Debussche L.
    J. Bacteriol. 172:5980-5990(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SC510.
  2. "The final step in the biosynthesis of hydrogenobyrinic acid is catalyzed by the cobH gene product with precorrin-8x as the substrate."
    Thibaut D., Couder M., Famechon A., Debussche L., Cameron B., Crouzet J., Blanche F.
    J. Bacteriol. 174:1043-1049(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-18, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
  3. Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 2-210 OF APOENZYME AND IN COMPLEX WITH HYDROGENOBYRINATE, SUBUNIT, ACTIVE SITE, REACTION MECHANISM.

Entry informationi

Entry nameiCOBH_PSEDE
AccessioniPrimary (citable) accession number: P21638
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: October 1, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3