P21638 (COBH_PSEDE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Precorrin-8X methylmutase
EC=5.4.1.2

Alternative name(s):
HBA synthase
Precorrin isomerase

Gene names

Name:

cobH

Organism

Pseudomonas denitrificans

Taxonomic identifier

43306 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas pertucinogena group

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of precorrin-8X to hydrogenobyrinic acid; a methyl migration reaction during the transformation of precorrin-3 to form cobyrinic acid.
Catalytic activity	Precorrin-8X = hydrogenobyrinate.
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 8/10.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the CobH family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Molecular function	Isomerase
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	precorrin-8X methylmutase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 210

210

Precorrin-8X methylmutase

PRO_0000135926

Sites

Active site

43

1

Secondary structure

1

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210

Helix Strand Turn

Details...

Helix

10 – 24

15

Beta strand

28 – 30

3

Helix

32 – 45

14

Helix

48 – 53

6

Beta strand

54 – 56

3

Helix

60 – 69

10

Beta strand

74 – 78

5

Helix

79 – 84

6

Helix

87 – 89

3

Beta strand

96 – 98

3

Helix

104 – 112

9

Helix

116 – 119

4

Helix

120 – 124

5

Helix

125 – 128

4

Beta strand

132 – 135

4

Helix

139 – 150

12

Beta strand

157 – 161

5

Beta strand

165 – 168

4

Helix

169 – 178

10

Beta strand

185 – 188

4

Helix

195 – 206

12

Sequences

	Sequence		Length	Mass (Da)
	P21638 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 506D1C4C5D972B3F	FASTA	210	22,064
	10 20 30 40 50 60 MPEYDYIRDG NAIYERSFAI IRAEADLSRF SEEEADLAVR MVHACGSVEA TRQFVFSPDF 70 80 90 100 110 120 VSSARAALKA GAPILCDAEM VAHGVTRARL PAGNEVICTL RDPRTPALAA EIGNTRSAAA 130 140 150 160 170 180 LKLWSERLAG SVVAIGNAPT ALFFLLEMLR DGAPKPAAIL GMPVGFVGAA ESKDALAENS 190 200 210 YGVPFAIVRG RLGGSAMTAA ALNSLARPGL

References

[1]	"Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans fragment carrying eight genes involved in transformation of precorrin-2 to cobyrinic acid." Crouzet J., Cameron B., Cauchois L., Rigault S., Rouyez M.-C., Blanche F., Thibaut D., Debussche L. J. Bacteriol. 172:5980-5990(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SC510.
[2]	"The final step in the biosynthesis of hydrogenobyrinic acid is catalyzed by the cobH gene product with precorrin-8x as the substrate." Thibaut D., Couder M., Famechon A., Debussche L., Cameron B., Crouzet J., Blanche F. J. Bacteriol. 174:1043-1049(1992) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[3]	"Crystal structure of precorrin-8x methyl mutase." Shipman L.W., Li D., Roessner C.A., Scott A.I., Sacchettini J.C. Structure 9:587-596(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 6-210.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M59301 Genomic DNA. Translation: AAA25796.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1F2V	X-ray	2.10	A	2-210	[»]
1I1H	X-ray	2.60	A	2-210	[»]

ProteinModelPortal

P21638.

SMR

P21638. Positions 2-210.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-115.

UniPathway

UPA00148; UER00219.

Family and domain databases

Gene3D

3.40.50.10230. 1 hit.

InterPro

IPR003722. Cbl_synth_CobH/CbiC_core.
[Graphical view]

Pfam

PF02570. CbiC. 1 hit.
[Graphical view]

SUPFAM

SSF63965. CbiC. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P21638.

Entry information

Entry name

COBH_PSEDE

Accession

Primary (citable) accession number: P21638

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program