P21637 (COBG_PSEDE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Precorrin-3B synthase
EC=1.14.13.83

Gene names

Name:

cobG

Organism

Pseudomonas denitrificans

Taxonomic identifier

43306 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the elimination of C-20 in precorrin-3A to form precorrin-3B.
Catalytic activity	Precorrin-3A + NADH + O2 = precorrin-3B + NAD+ + H2O.
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 2/10.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis Porphyrin biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW heme binding Inferred from electronic annotation. Source: InterPro precorrin-3B synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Precorrin-3B synthase		PRO_0000199966
Sites
	Metal binding	338	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	344	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	377	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	381	1	Iron (siroheme axial ligand) By similarity
	Metal binding	381	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21637 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: EC101F4C1FDC7209	FASTA	459	46,691
	10 20 30 40 50 60 MTDLMTSCAL PLTGDAGTVA SMRRGACPSL AEPMQTGDGL LVRVRPTDDS LTLPKVIALA 70 80 90 100 110 120 TAAERFGNGI IEITARGNLQ LRGLSAASVP RLAQAIGDAE IAIAEGLAIE VPPLAGIDPD 130 140 150 160 170 180 EIADPRPIAT ELREALDVRQ VPLKLAPKLS VVIDSGGRFG LGAVVADIRL QAVSTVAGVA 190 200 210 220 230 240 WVLSLGGTST KASSVGTLAG NAVVPALITI LEKLASLGTT MRGRDLDPSE IRALCRCETS 250 260 270 280 290 300 SERPAAPRSA AIPGIHALGN ADTVLGLGLA FAQVEAAALA SYLHQVQALG ANAIRLAPGH 310 320 330 340 350 360 AFFVLGLCPE TAAVAQSLAA SHGFRIAEQD PRNAIATCAG SKGCASAWME TKGMAERLVE 370 380 390 400 410 420 TAPELLDGSL TVHLSGCAKG CARPKPSELT LVGAPSGYGL VVNGAANGLP SAYTDENGMG 430 440 450 SALARLGRLV RQNKDAGESA QSCLTRLGAA RVSAAFEQG

References

[1]

"Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans fragment carrying eight genes involved in transformation of precorrin-2 to cobyrinic acid." Crouzet J., Cameron B., Cauchois L., Rigault S., Rouyez M.-C., Blanche F., Thibaut D., Debussche L. J. Bacteriol. 172:5980-5990(1990) [PubMed: 2211521] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SC510.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M59301 Genomic DNA. Translation: AAA25795.1.
3D structure databases
ProteinModelPortal	P21637.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-84.
Family and domain databases
InterPro	IPR012798. Cbl_synth_CobG-like. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. [Graphical view]
Gene3D	G3DSA:3.90.480.10. G3DSA:3.90.480.10. 2 hits.
Pfam	PF03460. NIR_SIR_ferr. 1 hit. [Graphical view]
SUPFAM	SSF55124. NiR_SiRalpha_1/3. 1 hit.
TIGRFAMs	TIGR02435. CobG. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COBG_PSEDE

Accession

Primary (citable) accession number: P21637

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	February 1, 1996
Last modified:	June 28, 2011
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program