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P21631

- SUMT_PSEDE

UniProt

P21631 - SUMT_PSEDE

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Protein

Uroporphyrinogen-III C-methyltransferase

Gene

cobA

Organism
Pseudomonas denitrificans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the methylation of both C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin.

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

Kineticsi

  1. KM=6.3 µM for S-adenosyl-L-methionine1 Publication
  2. KM=1.0 µM for uroporphyrinogen III1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei24 – 241S-adenosyl-L-methionine; via carbonyl oxygen1 Publication
Binding sitei130 – 1301S-adenosyl-L-methionine1 Publication
Binding sitei184 – 1841S-adenosyl-L-methionine; via amide nitrogen1 Publication

GO - Molecular functioni

  1. precorrin-2 dehydrogenase activity Source: InterPro
  2. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-UniPathway
  2. siroheme biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-82.
RETL1328306-WGS:GSTH-2506-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00262; UER00211.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
Short name:
Urogen III methylase
Alternative name(s):
SUMT
Uroporphyrinogen III methylase
Short name:
UROM
Gene namesi
Name:cobA
OrganismiPseudomonas denitrificans
Taxonomic identifieri43306 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi47 – 471D → N: Reduces S-adenosyl-L-methionine binding by about 75%. Causes accumulation of precorrin-1. 1 Publication
Mutagenesisi49 – 491L → A: Reduces S-adenosyl-L-methionine binding by about 50%. Causes slow synthesis of precorrin-2 and accumulation of precorrin-1. 1 Publication
Mutagenesisi106 – 1061F → A: Strongly reduces S-adenosyl-L-methionine binding. Loss of activity. 1 Publication
Mutagenesisi130 – 1301T → A: Strongly reduces S-adenosyl-L-methionine binding. Causes slow synthesis of precorrin-2. 1 Publication
Mutagenesisi183 – 1831Y → A: Strongly reduces S-adenosyl-L-methionine binding. Loss of activity. 1 Publication
Mutagenesisi184 – 1841M → A: Strongly reduces S-adenosyl-L-methionine binding. Causes drastic effects on enzyme activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 280280Uroporphyrinogen-III C-methyltransferasePRO_0000150372Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1
280
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi17 – 215Combined sources
Beta strandi23 – 253Combined sources
Beta strandi29 – 313Combined sources
Helixi32 – 409Combined sources
Beta strandi42 – 465Combined sources
Helixi54 – 574Combined sources
Beta strandi64 – 663Combined sources
Helixi78 – 9013Combined sources
Beta strandi95 – 1017Combined sources
Beta strandi105 – 1084Combined sources
Helixi109 – 1179Combined sources
Turni118 – 1203Combined sources
Beta strandi123 – 1264Combined sources
Turni131 – 1333Combined sources
Helixi134 – 1385Combined sources
Turni146 – 1483Combined sources
Beta strandi150 – 1567Combined sources
Helixi170 – 1745Combined sources
Beta strandi178 – 1847Combined sources
Helixi189 – 19810Combined sources
Beta strandi206 – 2127Combined sources
Beta strandi219 – 2246Combined sources
Turni225 – 2273Combined sources
Helixi228 – 2358Combined sources
Beta strandi239 – 2468Combined sources
Helixi247 – 2515Combined sources
Helixi252 – 2554Combined sources
Helixi257 – 2615Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S4DX-ray2.70A/B/D/E/F/G/H/I/J/K/L/M1-280[»]
ProteinModelPortaliP21631.
SMRiP21631. Positions 4-270.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21631.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 248248Uroporphyrinogen-III C-methyltransferaseAdd
BLAST
Regioni100 – 1023S-adenosyl-L-methionine binding

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.Curated

Family and domain databases

Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21631-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIDDLFAGLP ALEKGSVWLV GAGPGDPGLL TLHAANALRQ ADVIVHDALV
60 70 80 90 100
NEDCLKLARP GAVLEFAGKR GGKPSPKQRD ISLRLVELAR AGNRVLRLKG
110 120 130 140 150
GDPFVFGRGG EEALTLVEHQ VPFRIVPGIT AGIGGLAYAG IPVTHREVNH
160 170 180 190 200
AVTFLTGHDS SGLVPDRINW QGIASGSPVI VMYMAMKHIG AITANLIAGG
210 220 230 240 250
RSPDEPVAFV CNAATPQQAV LETTLARAEA DVAAAGLEPP AIVVVGEVVR
260 270 280
LRAALDWIGA LDGRKLAADP FANRILRNPA
Length:280
Mass (Da):29,253
Last modified:May 1, 1991 - v1
Checksum:i3D593C535CF0610E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59236 Genomic DNA. Translation: AAA25773.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59236 Genomic DNA. Translation: AAA25773.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1S4D X-ray 2.70 A/B/D/E/F/G/H/I/J/K/L/M 1-280 [» ]
ProteinModelPortali P21631.
SMRi P21631. Positions 4-270.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
UPA00262 ; UER00211 .
BioCyci MetaCyc:MONOMER-82.
RETL1328306-WGS:GSTH-2506-MONOMER.

Miscellaneous databases

EvolutionaryTracei P21631.

Family and domain databases

Gene3Di 3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProi IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view ]
Pfami PF00590. TP_methylase. 1 hit.
[Graphical view ]
SUPFAMi SSF53790. SSF53790. 1 hit.
TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITEi PS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of a Pseudomonas denitrificans 5.4-kilobase DNA fragment containing five cob genes and identification of structural genes encoding S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase and cobyrinic acid a,c-diamide synthase."
    Crouzet J., Cauchois L., Blanche F., Debussche L., Thibaut D., Rouyez M.-C., Rigault S., Mayaux J.-F., Cameron B.
    J. Bacteriol. 172:5968-5979(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
    Strain: SC510.
  2. "Purification and characterization of S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase from Pseudomonas denitrificans."
    Blanche F., Debussche L., Thibaut D., Crouzet J., Cameron B.
    J. Bacteriol. 171:4222-4231(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  3. "Structure/function studies on a S-adenosyl-L-methionine-dependent uroporphyrinogen III C methyltransferase (SUMT), a key regulatory enzyme of tetrapyrrole biosynthesis."
    Vevodova J., Graham R.M., Raux E., Schubert H.L., Roper D.I., Brindley A.A., Ian Scott A., Roessner C.A., Stamford N.P.J., Stroupe M.E., Getzoff E.D., Warren M.J., Wilson K.S.
    J. Mol. Biol. 344:419-433(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF APOPROTEIN IN COMPLEX WITH S-ADENOSYL-L-METHIONINE, SUBUNIT, KINETIC PARAMETERS, MUTAGENESIS OF ASP-47; LEU-49; PHE-106; THR-130; TYR-183 AND MET-184.

Entry informationi

Entry nameiSUMT_PSEDE
AccessioniPrimary (citable) accession number: P21631
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 26, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3