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Protein

Acetolactate synthase isozyme 3 small subunit

Gene

ilvH

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 pyruvate = 2-acetolactate + CO2.

Enzyme regulationi

Sensitive to valine inhibition.

Pathway:iL-isoleucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvB), Acetolactate synthase isozyme 3 large subunit (ilvI), Acetolactate synthase isozyme 3 small subunit (ilvH), Acetolactate synthase (ilvG)
  2. Ketol-acid reductoisomerase (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathway:iL-valine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-valine from pyruvate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvB), Acetolactate synthase isozyme 3 large subunit (ilvI), Acetolactate synthase isozyme 3 small subunit (ilvH), Acetolactate synthase (ilvG)
  2. Ketol-acid reductoisomerase (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Enzyme and pathway databases

BioCyciSENT99287:GCTI-115-MONOMER.
UniPathwayiUPA00047; UER00055.
UPA00049; UER00059.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetolactate synthase isozyme 3 small subunit (EC:2.2.1.6)
Alternative name(s):
ALS-III
Acetohydroxy-acid synthase III small subunit
Short name:
AHAS-III
Gene namesi
Name:ilvH
Ordered Locus Names:STM0117
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 163163Acetolactate synthase isozyme 3 small subunitPRO_0000151411Add
BLAST

Proteomic databases

PaxDbiP21622.
PRIDEiP21622.

Interactioni

Subunit structurei

Dimer of large and small chains.

Protein-protein interaction databases

STRINGi99287.STM0117.

Structurei

3D structure databases

ProteinModelPortaliP21622.
SMRiP21622. Positions 2-163.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 7875ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ACT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0440.
HOGENOMiHOG000046747.
KOiK01653.
OMAiRTGKIAM.
OrthoDBiEOG6X3W8W.
PhylomeDBiP21622.

Family and domain databases

InterProiIPR004789. Acetalactate_synth_ssu.
IPR019455. Acetolactate_synth_ssu_C.
IPR002912. ACT_dom.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF10369. ALS_ss_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00119. acolac_sm. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21622-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRILSVLLE NESGALSRVI GLFSQRGYNI ESLTVAPTDD PTLSRMTIQT
60 70 80 90 100
VGDEKVLEQI EKQLHKLVDV LRVSELGQGA HVEREIMLVK IQASGYGREE
110 120 130 140 150
VKRNTEIFRG QIIDVTPTLY TVQLAGTSDK LDAFLASLRD VAKIVEVARS
160
GVVGLSRGDK IMR
Length:163
Mass (Da):18,063
Last modified:January 23, 2002 - v2
Checksum:i82066840D433D6B5
GO

Sequence cautioni

The sequence AAF65177.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 922IQ → ME in CAA39102 (PubMed:1851954).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55456 Genomic DNA. Translation: CAA39102.1.
AF117227 Genomic DNA. Translation: AAF65177.1. Different initiation.
AE006468 Genomic DNA. Translation: AAL19081.1.
PIRiS15940.
RefSeqiNP_459122.1. NC_003197.1.
WP_001563086.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19081; AAL19081; STM0117.
GeneIDi1251635.
KEGGistm:STM0117.
PATRICi32378505. VBISalEnt20916_0122.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55456 Genomic DNA. Translation: CAA39102.1.
AF117227 Genomic DNA. Translation: AAF65177.1. Different initiation.
AE006468 Genomic DNA. Translation: AAL19081.1.
PIRiS15940.
RefSeqiNP_459122.1. NC_003197.1.
WP_001563086.1. NC_003197.1.

3D structure databases

ProteinModelPortaliP21622.
SMRiP21622. Positions 2-163.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM0117.

Proteomic databases

PaxDbiP21622.
PRIDEiP21622.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL19081; AAL19081; STM0117.
GeneIDi1251635.
KEGGistm:STM0117.
PATRICi32378505. VBISalEnt20916_0122.

Phylogenomic databases

eggNOGiCOG0440.
HOGENOMiHOG000046747.
KOiK01653.
OMAiRTGKIAM.
OrthoDBiEOG6X3W8W.
PhylomeDBiP21622.

Enzyme and pathway databases

UniPathwayiUPA00047; UER00055.
UPA00049; UER00059.
BioCyciSENT99287:GCTI-115-MONOMER.

Family and domain databases

InterProiIPR004789. Acetalactate_synth_ssu.
IPR019455. Acetolactate_synth_ssu_C.
IPR002912. ACT_dom.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF10369. ALS_ss_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00119. acolac_sm. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the ilvH-fruR gene region of Escherichia coli K12 and Salmonella typhimurium LT2."
    Jahreis K., Postma P.W., Lengeler J.W.
    Mol. Gen. Genet. 226:332-336(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  2. El Hanafi D., Maloriol D., Figueroa-Bossi N., Bossi L.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiILVH_SALTY
AccessioniPrimary (citable) accession number: P21622
Secondary accession number(s): Q9JP76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 23, 2002
Last modified: July 22, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

S.typhimurium contains genes for 3 AHAS isozymes: ilvBN, ilvGM and ilvIH.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.