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Protein

Acetolactate synthase isozyme 3 small subunit

Gene

ilvH

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Miscellaneous

S.typhimurium contains genes for 3 AHAS isozymes: ilvBN, ilvGM and ilvIH.

Catalytic activityi

2 pyruvate = 2-acetolactate + CO2.

Enzyme regulationi

Sensitive to valine inhibition.

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvB), Acetolactate synthase isozyme 3 large subunit (ilvI), Acetolactate synthase isozyme 3 small subunit (ilvH), Acetolactate synthase (ilvG)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-valine from pyruvate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvB), Acetolactate synthase isozyme 3 large subunit (ilvI), Acetolactate synthase isozyme 3 small subunit (ilvH), Acetolactate synthase (ilvG)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00047; UER00055.
UPA00049; UER00059.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetolactate synthase isozyme 3 small subunit (EC:2.2.1.6)
Alternative name(s):
ALS-III
Acetohydroxy-acid synthase III small subunit
Short name:
AHAS-III
Gene namesi
Name:ilvH
Ordered Locus Names:STM0117
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001514111 – 163Acetolactate synthase isozyme 3 small subunitAdd BLAST163

Proteomic databases

PaxDbiP21622.
PRIDEiP21622.

Interactioni

Subunit structurei

Dimer of large and small chains.

Protein-protein interaction databases

STRINGi99287.STM0117.

Structurei

3D structure databases

ProteinModelPortaliP21622.
SMRiP21622.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 78ACTPROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108ZP8. Bacteria.
COG0440. LUCA.
HOGENOMiHOG000046747.
KOiK01653.
OMAiRTGKIAM.
PhylomeDBiP21622.

Family and domain databases

InterProiView protein in InterPro
IPR004789. Acetalactate_synth_ssu.
IPR019455. Acetolactate_synth_ssu_C.
IPR002912. ACT_dom.
PANTHERiPTHR30239. PTHR30239. 1 hit.
PfamiView protein in Pfam
PF10369. ALS_ss_C. 1 hit.
TIGRFAMsiTIGR00119. acolac_sm. 1 hit.
PROSITEiView protein in PROSITE
PS51671. ACT. 1 hit.

Sequencei

Sequence statusi: Complete.

P21622-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRILSVLLE NESGALSRVI GLFSQRGYNI ESLTVAPTDD PTLSRMTIQT
60 70 80 90 100
VGDEKVLEQI EKQLHKLVDV LRVSELGQGA HVEREIMLVK IQASGYGREE
110 120 130 140 150
VKRNTEIFRG QIIDVTPTLY TVQLAGTSDK LDAFLASLRD VAKIVEVARS
160
GVVGLSRGDK IMR
Length:163
Mass (Da):18,063
Last modified:January 23, 2002 - v2
Checksum:i82066840D433D6B5
GO

Sequence cautioni

The sequence AAF65177 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti91 – 92IQ → ME in CAA39102 (PubMed:1851954).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55456 Genomic DNA. Translation: CAA39102.1.
AF117227 Genomic DNA. Translation: AAF65177.1. Different initiation.
AE006468 Genomic DNA. Translation: AAL19081.1.
PIRiS15940.
RefSeqiNP_459122.1. NC_003197.2.
WP_001563086.1. NC_003197.2.

Genome annotation databases

EnsemblBacteriaiAAL19081; AAL19081; STM0117.
GeneIDi1251635.
KEGGistm:STM0117.
PATRICifig|99287.12.peg.122.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiILVH_SALTY
AccessioniPrimary (citable) accession number: P21622
Secondary accession number(s): Q9JP76
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 23, 2002
Last modified: July 5, 2017
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families