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P21616 (AVP_VIGRR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 31, 2011. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyrophosphate-energized vacuolar membrane proton pump
EC=3.6.1.1
Alternative name(s):
Pyrophosphate-energized inorganic pyrophosphatase
Short name=H(+)-PPase
Vacuolar H(+)-pyrophosphatase
OrganismVigna radiata var. radiata (Mung bean) (Phaseolus aureus)
Taxonomic identifier3916 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Vigna

Protein attributes

Sequence length765 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Contributes to the transtonoplast (from cytosol to vacuole lumen) H+-electrochemical potential difference. It establishes a proton gradient of similar and often greater magnitude than the H+-ATPase on the same membrane.

Catalytic activity

Diphosphate + H2O = 2 phosphate.

Enzyme regulation

Inhibited by excess pyrophosphate as well as excess Mg2+. Inhibition by ATP, GTP, and CTP is reversed by increasing the Mg2+ concentration. This suggests that its substrate is a particular metal complex such as MgPPi2-.

Subunit structure

The requirement of phospholipid for its activity suggests that it is active in a phospholipid-protein complex.

Subcellular location

Vacuole membrane; Multi-pass membrane protein. Note: Tonoplast.

Sequence similarities

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily. [View classification]

Ontologies

Keywords
   Biological processHydrogen ion transport
Ion transport
Transport
   Cellular componentMembrane
Vacuole
   DomainTransmembrane
Transmembrane helix
   LigandMagnesium
   Molecular functionHydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processproton transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

vacuolar membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionhydrogen-translocating pyrophosphatase activity

Inferred from electronic annotation. Source: InterPro

inorganic diphosphatase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2
Chain2 – 765764Pyrophosphate-energized vacuolar membrane proton pump
PRO_0000217043

Regions

Topological domain2 – 1211Cytoplasmic Potential
Transmembrane13 – 3422Helical; Potential
Topological domain35 – 9460Intravacuolar Potential
Transmembrane95 – 11420Helical; Potential
Topological domain115 – 13521Cytoplasmic Potential
Transmembrane136 – 15924Helical; Potential
Topological domain160 – 18829Intravacuolar Potential
Transmembrane189 – 20921Helical; Potential
Topological domain210 – 22112Cytoplasmic Potential
Transmembrane222 – 24120Helical; Potential
Topological domain242 – 32079Intravacuolar Potential
Transmembrane321 – 34121Helical; Potential
Topological domain342 – 36423Cytoplasmic Potential
Transmembrane365 – 38622Helical; Potential
Topological domain387 – 40216Intravacuolar Potential
Transmembrane403 – 42220Helical; Potential
Topological domain423 – 45836Cytoplasmic Potential
Transmembrane459 – 48224Helical; Potential
Topological domain483 – 53856Intravacuolar Potential
Transmembrane539 – 56022Helical; Potential
Topological domain561 – 57414Cytoplasmic Potential
Transmembrane575 – 59420Helical; Potential
Topological domain595 – 65359Intravacuolar By similarity
Transmembrane654 – 67724Helical; Potential
Topological domain678 – 76588Cytoplasmic Potential

Sites

Active site2831Proton donor Probable

Experimental info

Sequence conflict221G → W AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
P21616 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: EECF39C48F735058

FASTA76579,980
        10         20         30         40         50         60 
MGAAILPDLG TEILIPVCAV IGIAFALFQW LLVSKVKLSA VRDASPNAAA KNGYNDYLIE 

        70         80         90        100        110        120 
EEEGINDHNV VVKCAEIQNA ISEGATSFLF TEYKYVGIFM VAFAILIFLF LGSVEGFSTS 

       130        140        150        160        170        180 
PQACSYDKTK TCKPALATAI FSTVSFLLGG VTSLVSGFLG MKIATYANAR TTLEARKGVG 

       190        200        210        220        230        240 
KAFITAFRSG AVMGFLLAAN GLLVLYIAIN LFKIYYGDDW GGLFEAITGY GLGGSSMALF 

       250        260        270        280        290        300 
GRVGGGIYTK AADVGADLVG KVERNIPEDD PRNPAVIADN VGDNVGDIAG MGSDLFGSYA 

       310        320        330        340        350        360 
ESSCAALVVA SISSFGLNHE LTAMLYPLIV SSVGILVCLL TTLFATDFFE IKAVKEIEPA 

       370        380        390        400        410        420 
LKKQLVISTV LMTIGVAVVS FVALPTSFTI FNFGVQKDVK SWQLFLCVAV GLWAGLIIGF 

       430        440        450        460        470        480 
VTEYYTSNAY SPVQDVADSC RTGAATNVIF GLALGYKSVI IPIFAIAISI FVSFTFAAMY 

       490        500        510        520        530        540 
GIAVAALGML STIATGLAID AYGPISDNAG GIAEMAGMSH RIRERTDALD AAGNTTAAIG 

       550        560        570        580        590        600 
KGFAIGSAAL VSLALFGAFV SRASITTVDV LTPKVFIGLI VGAMLPYWFS AMTMKSVGSA 

       610        620        630        640        650        660 
ALKMVEEVRR QFNTIPGLME GTAKPDYATC VKISTDASIK EMIPPGALVM LTPLVVGILF 

       670        680        690        700        710        720 
GVETLSGVLA GSLVSGVQIA ISASNTGAWD NAKKYIEAGA SEHARSLGPK GSDCHKAAVI 

       730        740        750        760 
GDTIGDPLKD TSGPSLNILI KLMAVESLVF APFFATHGGL LFKIF

« Hide

References

[1]"Vacuolar H+-pyrophosphatase cDNA from etiolated mung bean seedlings."
Hung S.-H., Chiu S.-J., Lin L.-Y., Pan R.L.
Plant Gene Register PGR95-082
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Hypocotyl.
[2]"Purification and properties of vacuolar membrane proton-translocating inorganic pyrophosphatase from mung bean."
Maeshima M., Yoshida S.
J. Biol. Chem. 264:20068-20073(1989) [PubMed: 2555340] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-31.
Strain: cv. Wilczek.
Tissue: Hypocotyl.
[3]"Localization of a carboxylic residue possibly involved in the inhibition of vacuolar H+-pyrophosphatase by N,N'-dicyclohexylcarbodi-imide."
Yang S.J., Jiang S.S., Kuo S.Y., Hung S.H., Tam M.F., Pan R.L.
Biochem. J. 342:641-646(1999) [PubMed: 10477275] [Abstract]
Cited for: ACTIVE SITE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U31467 mRNA. Translation: AAC49175.1.
PIRA34486.
T10841.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsTIGR01104. V_PPase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAVP_VIGRR
AccessionPrimary (citable) accession number: P21616
Secondary accession number(s): Q41686
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 23, 2007
Last modified: May 31, 2011
This is version 76 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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