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Protein

Vitamin D-binding protein

Gene

Gc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.

GO - Molecular functioni

  1. actin binding Source: UniProtKB
  2. calcidiol binding Source: MGI
  3. vitamin transporter activity Source: InterPro

GO - Biological processi

  1. vitamin D metabolic process Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Actin-binding, Vitamin D

Enzyme and pathway databases

ReactomeiREACT_218811. Vitamin D (calciferol) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin D-binding protein
Short name:
DBP
Short name:
VDB
Alternative name(s):
Gc-globulin
Group-specific component
Gene namesi
Name:Gc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:95669. Gc.

Subcellular locationi

GO - Cellular componenti

  1. blood microparticle Source: MGI
  2. extracellular space Source: MGI
  3. extracellular vesicular exosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 16161 PublicationAdd
BLAST
Chaini17 – 476460Vitamin D-binding proteinPRO_0000001103Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi29 ↔ 75PROSITE-ProRule annotation
Disulfide bondi74 ↔ 83PROSITE-ProRule annotation
Disulfide bondi96 ↔ 112PROSITE-ProRule annotation
Disulfide bondi111 ↔ 122PROSITE-ProRule annotation
Disulfide bondi145 ↔ 190PROSITE-ProRule annotation
Disulfide bondi189 ↔ 198PROSITE-ProRule annotation
Disulfide bondi220 ↔ 266PROSITE-ProRule annotation
Disulfide bondi265 ↔ 273PROSITE-ProRule annotation
Disulfide bondi286 ↔ 300PROSITE-ProRule annotation
Glycosylationi288 – 2881N-linked (GlcNAc...)1 Publication
Disulfide bondi299 ↔ 311PROSITE-ProRule annotation
Disulfide bondi335 ↔ 376PROSITE-ProRule annotation
Disulfide bondi375 ↔ 384PROSITE-ProRule annotation
Disulfide bondi407 ↔ 453PROSITE-ProRule annotation
Disulfide bondi452 ↔ 462PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP21614.
PaxDbiP21614.
PRIDEiP21614.

2D gel databases

REPRODUCTION-2DPAGEP21614.

PTM databases

PhosphoSiteiP21614.

Expressioni

Gene expression databases

BgeeiP21614.
CleanExiMM_GC.
GenevestigatoriP21614.

Interactioni

Protein-protein interaction databases

BioGridi199850. 1 interaction.
IntActiP21614. 5 interactions.
MINTiMINT-1857787.

Structurei

3D structure databases

ProteinModelPortaliP21614.
SMRiP21614. Positions 20-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 208192Albumin 1PROSITE-ProRule annotationAdd
BLAST
Domaini209 – 394186Albumin 2PROSITE-ProRule annotationAdd
BLAST
Domaini395 – 47682Albumin 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ALB/AFP/VDB family.PROSITE-ProRule annotation
Contains 3 albumin domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG40465.
GeneTreeiENSGT00390000000113.
HOGENOMiHOG000140946.
HOVERGENiHBG009729.
InParanoidiP21614.
KOiK12258.
OMAiYEYSINY.
OrthoDBiEOG7B05CR.
PhylomeDBiP21614.
TreeFamiTF335561.

Family and domain databases

InterProiIPR000264. ALB/AFP/VDB.
IPR020858. Serum_albumin-like.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
IPR000213. VitD-bd.
IPR015247. VitD-bind_III.
[Graphical view]
PfamiPF00273. Serum_albumin. 2 hits.
PF09164. VitD-bind_III. 1 hit.
[Graphical view]
PRINTSiPR00802. SERUMALBUMIN.
PR00804. VITAMNDBNDNG.
SMARTiSM00103. ALBUMIN. 2 hits.
[Graphical view]
SUPFAMiSSF48552. SSF48552. 3 hits.
PROSITEiPS00212. ALBUMIN_1. 1 hit.
PS51438. ALBUMIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21614-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRVLVLLLA LAFGHALERG RDYEKDKVCN ELAMLGKEDF RSLSLILYSR
60 70 80 90 100
KFSSSTFEQV NQLVKEVVSL TEECCAEGAD PTCYDTRTSE LSVKSCESDA
110 120 130 140 150
PFPVHPGTPE CCTKEGLERK LCMAALSHQP QEFPTYVEPT NDEICEAFRR
160 170 180 190 200
DPKGFADQFL YEYSSNYGQA PLPLLVAYTK NYLSMVGSCC TSANPTVCFV
210 220 230 240 250
KERLQMKHLS LLTTMSNRVC SQYAAYGKEK SRLSHLIKLA QKVPTANLEN
260 270 280 290 300
VLPLAEDFTE ILSRCCESTS EDCMASELPE HTIKICQNLS KKNSKFEECC
310 320 330 340 350
QENTPMNIFM CTYFMPAAEP LQLPAIKLPT GKDLCGQSTT QAMDQYTFEL
360 370 380 390 400
SRRTQVPEVF LSKVLEPTLK TLRECCDTQD SVACFSTQSP LLKRQLTSFI
410 420 430 440 450
EKGQEMCADY SENTFTEYKK KLAERLRTKT PNTSPAELKD MVEKHSDFAS
460 470
KCCSINSPPL YCSSQIDAEM IDTLQS
Length:476
Mass (Da):53,600
Last modified:October 10, 2003 - v2
Checksum:i633B0CE183CD43FD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti247 – 2471N → K in BAB27297. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010965 mRNA. Translation: BAB27297.1.
BC010762 mRNA. Translation: AAH10762.1.
BC051395 mRNA. Translation: AAH51395.2.
M55413 mRNA. Translation: AAA37669.1.
CCDSiCCDS19407.1.
PIRiA35327.
RefSeqiNP_032122.1. NM_008096.2.
UniGeneiMm.196595.

Genome annotation databases

EnsembliENSMUST00000049209; ENSMUSP00000046636; ENSMUSG00000035540.
GeneIDi14473.
KEGGimmu:14473.
UCSCiuc008yam.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010965 mRNA. Translation: BAB27297.1.
BC010762 mRNA. Translation: AAH10762.1.
BC051395 mRNA. Translation: AAH51395.2.
M55413 mRNA. Translation: AAA37669.1.
CCDSiCCDS19407.1.
PIRiA35327.
RefSeqiNP_032122.1. NM_008096.2.
UniGeneiMm.196595.

3D structure databases

ProteinModelPortaliP21614.
SMRiP21614. Positions 20-474.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199850. 1 interaction.
IntActiP21614. 5 interactions.
MINTiMINT-1857787.

PTM databases

PhosphoSiteiP21614.

2D gel databases

REPRODUCTION-2DPAGEP21614.

Proteomic databases

MaxQBiP21614.
PaxDbiP21614.
PRIDEiP21614.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049209; ENSMUSP00000046636; ENSMUSG00000035540.
GeneIDi14473.
KEGGimmu:14473.
UCSCiuc008yam.2. mouse.

Organism-specific databases

CTDi2638.
MGIiMGI:95669. Gc.

Phylogenomic databases

eggNOGiNOG40465.
GeneTreeiENSGT00390000000113.
HOGENOMiHOG000140946.
HOVERGENiHBG009729.
InParanoidiP21614.
KOiK12258.
OMAiYEYSINY.
OrthoDBiEOG7B05CR.
PhylomeDBiP21614.
TreeFamiTF335561.

Enzyme and pathway databases

ReactomeiREACT_218811. Vitamin D (calciferol) metabolism.

Miscellaneous databases

ChiTaRSiGc. mouse.
NextBioi286142.
PROiP21614.
SOURCEiSearch...

Gene expression databases

BgeeiP21614.
CleanExiMM_GC.
GenevestigatoriP21614.

Family and domain databases

InterProiIPR000264. ALB/AFP/VDB.
IPR020858. Serum_albumin-like.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
IPR000213. VitD-bd.
IPR015247. VitD-bind_III.
[Graphical view]
PfamiPF00273. Serum_albumin. 2 hits.
PF09164. VitD-bind_III. 1 hit.
[Graphical view]
PRINTSiPR00802. SERUMALBUMIN.
PR00804. VITAMNDBNDNG.
SMARTiSM00103. ALBUMIN. 2 hits.
[Graphical view]
SUPFAMiSSF48552. SSF48552. 3 hits.
PROSITEiPS00212. ALBUMIN_1. 1 hit.
PS51438. ALBUMIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryonic liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney and Liver.
  3. "Mapping and conservation of the group-specific component gene in mouse."
    Yang F., Bergeron J.M., Linehan L.A., Lalley P.A., Sakaguchi A.Y., Bowman B.H.
    Genomics 7:509-516(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-476.
  4. "The isolation, characterization and amino terminal sequence of the vitamin D-binding protein (group specific component) from mouse plasma."
    Borke J.L., Litwiller R.D., Bell M.P., Fass D.N., McKean D.J., Kumar R.
    Int. J. Biochem. 20:1343-1349(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 17-38.
  5. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-288.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiVTDB_MOUSE
AccessioniPrimary (citable) accession number: P21614
Secondary accession number(s): Q7TS97, Q91XG1, Q9CY31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: October 10, 2003
Last modified: February 4, 2015
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.