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P21599

- KPYK2_ECOLI

UniProt

P21599 - KPYK2_ECOLI

Protein

Pyruvate kinase II

Gene

pykA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.
    Potassium.

    Enzyme regulationi

    Allosterically activated by AMP and by several sugar phosphates. Belongs to type II PK.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei36 – 361SubstrateBy similarity
    Metal bindingi38 – 381PotassiumBy similarity
    Metal bindingi40 – 401PotassiumBy similarity
    Metal bindingi70 – 701PotassiumBy similarity
    Sitei223 – 2231Transition state stabilizerBy similarity
    Metal bindingi225 – 2251MagnesiumBy similarity
    Binding sitei251 – 2511Substrate; via amide nitrogenBy similarity
    Metal bindingi252 – 2521MagnesiumBy similarity
    Binding sitei252 – 2521Substrate; via amide nitrogenBy similarity
    Binding sitei284 – 2841SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. identical protein binding Source: IntAct
    3. magnesium ion binding Source: InterPro
    4. potassium ion binding Source: InterPro
    5. protein binding Source: IntAct
    6. pyruvate kinase activity Source: EcoCyc

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    BioCyciEcoCyc:PKII-MONOMER.
    ECOL316407:JW1843-MONOMER.
    MetaCyc:PKII-MONOMER.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase II (EC:2.7.1.40)
    Alternative name(s):
    PK-2
    Gene namesi
    Name:pykA
    Ordered Locus Names:b1854, JW1843
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10803. pykA.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 480479Pyruvate kinase IIPRO_0000112073Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei351 – 3511N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP21599.
    PRIDEiP21599.

    Expressioni

    Gene expression databases

    GenevestigatoriP21599.

    Interactioni

    Subunit structurei

    Homotetramer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself3EBI-368956,EBI-368956
    malXP196423EBI-368956,EBI-556578
    yggRP520523EBI-368956,EBI-552531

    Protein-protein interaction databases

    DIPiDIP-10622N.
    IntActiP21599. 15 interactions.
    MINTiMINT-1238649.
    STRINGi511145.b1854.

    Structurei

    3D structure databases

    ProteinModelPortaliP21599.
    SMRiP21599. Positions 4-476.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021558.
    KOiK00873.
    OMAiTPVYFDS.
    OrthoDBiEOG6GBMB0.
    PhylomeDBiP21599.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P21599-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRRLRRTKI VTTLGPATDR DNNLEKVIAA GANVVRMNFS HGSPEDHKMR    50
    ADKVREIAAK LGRHVAILGD LQGPKIRVST FKEGKVFLNI GDKFLLDANL 100
    GKGEGDKEKV GIDYKGLPAD VVPGDILLLD DGRVQLKVLE VQGMKVFTEV 150
    TVGGPLSNNK GINKLGGGLS AEALTEKDKA DIKTAALIGV DYLAVSFPRC 200
    GEDLNYARRL ARDAGCDAKI VAKVERAEAV CSQDAMDDII LASDVVMVAR 250
    GDLGVEIGDP ELVGIQKALI RRARQLNRAV ITATQMMESM ITNPMPTRAE 300
    VMDVANAVLD GTDAVMLSAE TAAGQYPSET VAAMARVCLG AEKIPSINVS 350
    KHRLDVQFDN VEEAIAMSAM YAANHLKGVT AIITMTESGR TALMTSRISS 400
    GLPIFAMSRH ERTLNLTALY RGVTPVHFDS ANDGVAAASE AVNLLRDKGY 450
    LMSGDLVIVT QGDVMSTVGS TNTTRILTVE 480
    Length:480
    Mass (Da):51,357
    Last modified:January 23, 2007 - v3
    Checksum:iC37F004C374D27E9
    GO

    Sequence cautioni

    The sequence AAA96707.1 differs from that shown. Reason: Frameshift at position 441.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti20 – 201R → C AA sequence (PubMed:1859631)Curated
    Sequence conflicti43 – 431S → G AA sequence (PubMed:1859631)Curated
    Sequence conflicti323 – 3231A → R in AAA96707. 1 PublicationCurated
    Sequence conflicti383 – 3831Missing in AAA96707. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63703 Genomic DNA. Translation: AAA24473.1.
    U00096 Genomic DNA. Translation: AAC74924.1.
    AP009048 Genomic DNA. Translation: BAA15662.1.
    M77039 Genomic DNA. No translation available.
    M87660 Genomic DNA. Translation: AAA96707.1. Frameshift.
    PIRiS29790.
    RefSeqiNP_416368.1. NC_000913.3.
    YP_490116.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74924; AAC74924; b1854.
    BAA15662; BAA15662; BAA15662.
    GeneIDi12934211.
    946527.
    KEGGiecj:Y75_p1830.
    eco:b1854.
    PATRICi32119031. VBIEscCol129921_1933.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63703 Genomic DNA. Translation: AAA24473.1 .
    U00096 Genomic DNA. Translation: AAC74924.1 .
    AP009048 Genomic DNA. Translation: BAA15662.1 .
    M77039 Genomic DNA. No translation available.
    M87660 Genomic DNA. Translation: AAA96707.1 . Frameshift.
    PIRi S29790.
    RefSeqi NP_416368.1. NC_000913.3.
    YP_490116.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P21599.
    SMRi P21599. Positions 4-476.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10622N.
    IntActi P21599. 15 interactions.
    MINTi MINT-1238649.
    STRINGi 511145.b1854.

    Proteomic databases

    PaxDbi P21599.
    PRIDEi P21599.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74924 ; AAC74924 ; b1854 .
    BAA15662 ; BAA15662 ; BAA15662 .
    GeneIDi 12934211.
    946527.
    KEGGi ecj:Y75_p1830.
    eco:b1854.
    PATRICi 32119031. VBIEscCol129921_1933.

    Organism-specific databases

    EchoBASEi EB0796.
    EcoGenei EG10803. pykA.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000021558.
    KOi K00873.
    OMAi TPVYFDS.
    OrthoDBi EOG6GBMB0.
    PhylomeDBi P21599.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    BioCyci EcoCyc:PKII-MONOMER.
    ECOL316407:JW1843-MONOMER.
    MetaCyc:PKII-MONOMER.

    Miscellaneous databases

    PROi P21599.

    Gene expression databases

    Genevestigatori P21599.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Bledig S.A., Fotheringham I.G., Hunter M.G.
      Submitted (AUG-1991) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Isolation and characterization of the Escherichia coli msbB gene, a multicopy suppressor of null mutations in the high-temperature requirement gene htrB."
      Karow M.L., Georgopoulos C.
      J. Bacteriol. 174:702-710(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 319-480.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. Engel H., Smink A.J., Keck W.
      Submitted (MAR-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 323-480.
      Strain: K12.
    7. "Bacterial pyruvate kinases have a shorter N-terminal domain."
      Valentini G., Stoppini M., Speranza M.L., Malcovati M., Ferri G.
      Biol. Chem. Hoppe-Seyler 372:91-93(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-44.
    8. "Divergent binding sites in pyruvate kinases I and II from Escherichia coli."
      Valentini G., Stoppini M., Iadarola P., Malcovati M., Ferri G., Speranza M.L.
      Biol. Chem. Hoppe-Seyler 374:69-74(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 64-77; 83-107; 251-272 AND 337-353.
    9. "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli."
      Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.
      Mol. Cell. Proteomics 8:215-225(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-351, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: K12 / JW1106 and K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiKPYK2_ECOLI
    AccessioniPrimary (citable) accession number: P21599
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 140 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3