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Protein

Orotidine 5'-phosphate decarboxylase

Gene

pyrG

Organism
Phycomyces blakesleeanus (strain ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Orotidine 5'-phosphate = UMP + CO2.PROSITE-ProRule annotation

Pathway: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes UMP from orotate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Orotidine 5'-phosphate decarboxylase (pyrG)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401SubstrateBy similarity
Active sitei95 – 951Proton donorPROSITE-ProRule annotation
Binding sitei215 – 2151SubstrateBy similarity
Binding sitei234 – 2341SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Pyrimidine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00070; UER00120.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotidine 5'-phosphate decarboxylase (EC:4.1.1.23)
Alternative name(s):
OMP decarboxylase
Short name:
OMPDCase
Short name:
OMPdecase
Uridine 5'-monophosphate synthase
Short name:
UMP synthase
Gene namesi
Name:pyrG
OrganismiPhycomyces blakesleeanus (strain ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555)
Taxonomic identifieri763407 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisEarly diverging fungal lineagesMucoromycotinaMucoralesPhycomycetaceaePhycomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267Orotidine 5'-phosphate decarboxylasePRO_0000134671Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP21593.
SMRiP21593. Positions 8-262.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni62 – 643Substrate bindingBy similarity
Regioni93 – 10210Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the OMP decarboxylase family.Curated

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR014732. OMPdecase.
IPR018089. OMPdecase_AS.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01740. pyrF. 1 hit.
PROSITEiPS00156. OMPDECASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21593-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLNTYKSYT ERAEQHPNAC ARSLFELMER KKTNLSVAVD VTTKKELLSI
60 70 80 90 100
ADSVGPYVCV LKTHIDIVED FDKDLVAQLE ALAKKHDFLI FEDRKFADIG
110 120 130 140 150
NTVKHQYEKG VYKIASWSHI TNAHTVPGEG IIKGLGEVGL PLGRGLLLLA
160 170 180 190 200
EMSSKGALTK GSYTTESVEM ARRNKDFVFG FIAQHKMNEY PDEDFVVMTP
210 220 230 240 250
GVGLDIKGDG LGQQYRTPHE VIVESGCDVI IVGRGIYGKP DEVEAQSKRY
260
REAGWNAYLE RVRMHKA
Length:267
Mass (Da):29,899
Last modified:May 1, 1991 - v1
Checksum:iCE5B132327F3FDDC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53601 Genomic DNA. Translation: CAA37670.1.
PIRiS13736. DCUMOP.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53601 Genomic DNA. Translation: CAA37670.1.
PIRiS13736. DCUMOP.

3D structure databases

ProteinModelPortaliP21593.
SMRiP21593. Positions 8-262.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00070; UER00120.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR014732. OMPdecase.
IPR018089. OMPdecase_AS.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01740. pyrF. 1 hit.
PROSITEiPS00156. OMPDECASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and molecular analysis of the orotidine-5'-phosphate decarboxylase gene (pyrG) of Phycomyces blakesleeanus."
    Diaz-Minguez J.M., Iturriaga E.A., Benito E.P., Corrochano L., Eslava A.P.
    Mol. Gen. Genet. 224:269-278(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555.

Entry informationi

Entry nameiPYRF_PHYB8
AccessioniPrimary (citable) accession number: P21593
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: October 1, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.