Reviewed,
UniProtKB/Swiss-Prot P21589 (5NTD_HUMAN)
Last modified
February 9, 2010.
Version 108.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 5'-nucleotidase Short name=5'-NT EC=3.1.3.5 Alternative name(s): Ecto-5'-nucleotidase CD_antigen=CD73 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 574 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. |
| Catalytic activity | A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. |
| Cofactor | Zinc. |
| Subunit structure | Homodimer; disulfide-linked. |
| Subcellular location | |
| Sequence similarities | Belongs to the 5'-nucleotidase family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Coding sequence diversity | Polymorphism |
| Domain | Signal |
| Ligand | Metal-binding Nucleotide-binding Zinc |
| Molecular function | Hydrolase |
| PTM | Disulfide bond GPI-anchor Glycoprotein Lipoprotein |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | DNA metabolic process Ref.1 Traceable author statement. Source: ProtInc |
| Cellular component | anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmInferred from direct assay. Source: HPA membrane fraction Ref.1Traceable author statement. Source: ProtInc plasma membraneInferred from direct assay. Source: HPA |
| Molecular function | 5'-nucleotidase activity Ref.1 Inferred from Experiment. Source: Reactome nucleotide bindingInferred from electronic annotation. Source: UniProtKB-KW zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 26 | 26 | Ref.5 | ||||||
| Chain | 27 – 549 | 523 | 5'-nucleotidase Ref.1 | PRO_0000000015 | |||||
| Propeptide | 550 – 574 | 25 | Removed in mature form | PRO_0000000016 | |||||
Regions | |||||||||
| Region | 500 – 506 | 7 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Metal binding | 36 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 38 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 85 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 85 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 117 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 220 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 243 | 1 | Zinc 2 By similarity | ||||||
| Binding site | 417 | 1 | Substrate By similarity | ||||||
| Site | 118 | 1 | Transition state stabilizer By similarity | ||||||
| Site | 121 | 1 | Transition state stabilizer By similarity | ||||||
Amino acid modifications | |||||||||
| Lipidation | 549 | 1 | GPI-anchor amidated serine Ref.1 | ||||||
| Glycosylation | 53 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 311 | 1 | N-linked (GlcNAc...) Ref.6 | ||||||
| Glycosylation | 333 | 1 | N-linked (GlcNAc...) Ref.6 Ref.7 | ||||||
| Glycosylation | 403 | 1 | N-linked (GlcNAc...) Ref.6 | ||||||
Natural variations | |||||||||
| Natural variant | 376 | 1 | T → A: dbSNP rs2229523. Ref.4 | VAR_022091 | |||||
| Natural variant | 379 | 1 | M → T: dbSNP rs2229524. | VAR_048103 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form." Misumi Y., Ogata S., Ohkubo K., Hirose S., Ikehara Y. Eur. J. Biochem. 191:563-569(1990) [PubMed: 2129526] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, GPI-ANCHOR AT SER-549. Tissue: Placenta. |
| [2] | "The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. Beck S.Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "Isolation and characterization of the promoter of the human 5'-nucleotidase (CD73)-encoding gene." Hansen K.R., Resta R., Webb C.F., Thompson L.F. Gene 167:307-312(1995) [PubMed: 8566797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-113. Tissue: Placenta. |
| [4] | Zanoni L., Rosi F., Pagani R., Marinello E. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 359-489, VARIANT ALA-376. Tissue: Leukocyte. |
| [5] | "Characterization of soluble vs membrane-bound human placental 5'-nucleotidase." Klemens M.R., Sherman W.R., Holmberg N.J., Ruedi J.M., Low M.G., Thompson L.F. Biochem. Biophys. Res. Commun. 172:1371-1377(1990) [PubMed: 2173922] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-40. Tissue: Placenta. |
| [6] | "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-311; ASN-333 AND ASN-403, MASS SPECTROMETRY. Tissue: Liver. |
| [7] | "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-333, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X55740 mRNA. Translation: CAA39271.1. AL135903, AL589666 Genomic DNA. Translation: CAH72337.1. AL589666, AL135903 Genomic DNA. Translation: CAI40168.1. U21730 Genomic DNA. Translation: AAA96950.1. AF069067 Genomic DNA. Translation: AAC98672.1. |
| IPI | IPI00009456. |
| PIR | S11032. |
| RefSeq | NP_002517.1. |
| UniGene | Hs.153952 |
3D structure databases | |
| SMR | P21589. Positions 27-549. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P21589. |
Proteomic databases | |
| PRIDE | P21589. |
Genome annotation databases | |
| Ensembl | ENST00000257770; ENSP00000257770; ENSG00000135318; Homo sapiens. [Genome view] |
| GeneID | 4907. |
| KEGG | hsa:4907. |
| UCSC | uc003pko.2. human. |
Organism-specific databases | |
| CTD | 4907. |
| GeneCards | GC06P086216. |
| H-InvDB | HIX0032840. |
| HGNC | HGNC:8021. NT5E. |
| HPA | HPA017357. |
| MIM | 129190. gene. |
| PharmGKB | PA31804. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG17669. |
| HOGENOM | HBG534106. |
| HOVERGEN | P21589. |
| InParanoid | P21589. |
| OMA | KDELLRH. |
| OrthoDB | EOG92NMKC. |
| PhylomeDB | P21589. |
Enzyme and pathway databases | |
| BRENDA | 3.1.3.5. 247. |
| Pathway_Interaction_DB | hif1_tfpathway. HIF-1-alpha transcription factor network. |
| Reactome | REACT_1698. Metabolism of nucleotides. |
Gene expression databases | |
| ArrayExpress | P21589. |
| Bgee | P21589. |
| CleanEx | HS_NT5E. |
| Genevestigator | P21589. |
| GermOnline | ENSG00000135318. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR008334. 5'-Nucleotdase_C. IPR006146. 5'-Nucleotdase_CS. IPR006179. 5_nucleotidase/apyrase. IPR004843. M-pesterase. [Graphical view] |
| Gene3D | G3DSA:3.90.780.10. 5'-Nucleotdase_C. 1 hit. |
| PANTHER | PTHR11575. 5_nucleotidase. 1 hit. |
| Pfam | PF02872. 5_nucleotid_C. 1 hit. PF00149. Metallophos. 1 hit. [Graphical view] |
| PRINTS | PR01607. APYRASEFAMLY. |
| PROSITE | PS00785. 5_NUCLEOTIDASE_1. 1 hit. PS00786. 5_NUCLEOTIDASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| DrugBank | DB00806. Pentoxifylline. |
| NextBio | 18883. |
| SOURCE | Search... |
Entry information
| Entry name | 5NTD_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P21589 Secondary accession number(s): O75520, Q5W116 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human cell differentiation molecules CD nomenclature of surface proteins of human leucocytes and list of entries |
| Human chromosome 6 Human chromosome 6: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

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