5'-nucleotidase precursor - Homo sapiens (Human)

Names and origin

Protein names

Recommended name:
    5'-nucleotidase
    EC=3.1.3.5
Alternative name(s):
    Ecto-5'-nucleotidase
      Short name=5'-NT
    CD_antigen=CD73

Gene names

Name:	NT5E
Synonyms:	NT5, NTE

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Catalytic activity	A 5'-ribonucleotide + H₂O = a ribonucleoside + phosphate.
Cofactor	Zinc.
Subunit structure	Homodimer; disulfide-linked.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor. Ref.1
Involvement in disease	There is a decrease in the activity of NT5 in B-cell chronic lymphocytic leukemia. Ref.6
Sequence similarities	Belongs to the 5'-nucleotidase family.

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Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Signal
Ligand	Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA metabolic process Ref.1 Traceable author statement. Source: ProtInc nucleotide catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane fraction Ref.1 Traceable author statement. Source: ProtInc plasma membrane Inferred from Experiment. Source: Reactome
Molecular function	5'-nucleotidase activity Ref.1 Inferred from Experiment. Source: Reactome nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

26

Ref.5

Chain

27 – 549

523

5'-nucleotidase Ref.1

PRO_0000000015

Propeptide

550 – 574

25

Removed in mature form

PRO_0000000016

Regions

Region

500 – 506

7

Substrate binding By similarity

Sites

Metal binding

36

1

Zinc 1 By similarity

Metal binding

38

1

Zinc 1 By similarity

Metal binding

85

1

Zinc 1 By similarity

Metal binding

85

1

Zinc 2 By similarity

Metal binding

117

1

Zinc 2 By similarity

Metal binding

220

1

Zinc 2 By similarity

Metal binding

243

1

Zinc 2 By similarity

Binding site

417

1

Substrate By similarity

Site

118

1

Transition state stabilizer By similarity

Site

121

1

Transition state stabilizer By similarity

Amino acid modifications

Lipidation

549

1

GPI-anchor amidated serine Ref.1

Glycosylation

53

1

N-linked (GlcNAc...) Potential

Glycosylation

311

1

N-linked (GlcNAc...)

Glycosylation

333

1

N-linked (GlcNAc...)

Glycosylation

403

1

N-linked (GlcNAc...)

Natural variations

Natural variant

376

1

T → A: dbSNP rs2229523. Ref.4

VAR_022091

Natural variant

379

1

M → T: dbSNP rs2229524.

VAR_048103

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Sequences

Sequence

Length

Mass (Da)

Tools

P21589-1 [UniParc].

Last modified May 1, 1991. Version 1.
Checksum: A99AF170AB7EAECE
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FASTA

574

63,368

        10         20         30         40         50         60 
MCPRAARAPA TLLLALGAVL WPAAGAWELT ILHTNDVHSR LEQTSEDSSK CVNASRCMGG 

        70         80         90        100        110        120 
VARLFTKVQQ IRRAEPNVLL LDAGDQYQGT IWFTVYKGAE VAHFMNALRY DAMALGNHEF 

       130        140        150        160        170        180 
DNGVEGLIEP LLKEAKFPIL SANIKAKGPL ASQISGLYLP YKVLPVGDEV VGIVGYTSKE 

       190        200        210        220        230        240 
TPFLSNPGTN LVFEDEITAL QPEVDKLKTL NVNKIIALGH SGFEMDKLIA QKVRGVDVVV 

       250        260        270        280        290        300 
GGHSNTFLYT GNPPSKEVPA GKYPFIVTSD DGRKVPVVQA YAFGKYLGYL KIEFDERGNV 

       310        320        330        340        350        360 
ISSHGNPILL NSSIPEDPSI KADINKWRIK LDNYSTQELG KTIVYLDGSS QSCRFRECNM 

       370        380        390        400        410        420 
GNLICDAMIN NNLRHTDEMF WNHVSMCILN GGGIRSPIDE RNNGTITWEN LAAVLPFGGT 

       430        440        450        460        470        480 
FDLVQLKGST LKKAFEHSVH RYGQSTGEFL QVGGIHVVYD LSRKPGDRVV KLDVLCTKCR 

       490        500        510        520        530        540 
VPSYDPLKMD EVYKVILPNF LANGGDGFQM IKDELLRHDS GDQDINVVST YISKMKVIYP 

       550        560        570 
AVEGRIKFST GSHCHGSFSL IFLSLWAVIF VLYQ

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form." Misumi Y., Ogata S., Ohkubo K., Hirose S., Ikehara Y. Eur. J. Biochem. 191:563-569(1990) [PubMed: 2129526] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, GPI-ANCHOR AT SER-549. Tissue: Placenta.
[2]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Isolation and characterization of the promoter of the human 5'-nucleotidase (CD73)-encoding gene." Hansen K.R., Resta R., Webb C.F., Thompson L.F. Gene 167:307-312(1995) [PubMed: 8566797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-113. Tissue: Placenta.
[4]	Zanoni L., Rosi F., Pagani R., Marinello E. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 359-489, VARIANT ALA-376. Tissue: Leukocyte.
[5]	"Characterization of soluble vs membrane-bound human placental 5'-nucleotidase." Klemens M.R., Sherman W.R., Holmberg N.J., Ruedi J.M., Low M.G., Thompson L.F. Biochem. Biophys. Res. Commun. 172:1371-1377(1990) [PubMed: 2173922] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-40. Tissue: Placenta.
[6]	"Behaviour of human lymphocytic isoenzymes of 5'-nucleotidase." Rosi F., Agostinho A.B., Carlucci F., Zanoni L., Porcelli B., Marinello E., Galieni P., Tabucchi A. Life Sci. 62:2257-2266(1998) [PubMed: 9651114] [Abstract] Cited for: DISEASE.
[7]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-311; ASN-333 AND ASN-403, MASS SPECTROMETRY. Tissue: Liver.
[8]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-333, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	X55740 mRNA. Translation: CAA39271.1. AL135903, AL589666 Genomic DNA. Translation: CAH72337.1. AL589666, AL135903 Genomic DNA. Translation: CAI40168.1. U21730 Genomic DNA. Translation: AAA96950.1. AF069067 Genomic DNA. Translation: AAC98672.1.
IPI	IPI00009456.
PIR	S11032.
RefSeq	NP_002517.1.
UniGene	Hs.153952
3D structure databases
HSSP	HSSP built from PDB template 2USH based on UniProtKB P07024.
ModBase	Search...
Proteomic databases
PRIDE	P21589.
Genome annotation databases
Ensembl	ENSG00000135318. Homo sapiens. [Contig view]
GeneID	4907.
KEGG	hsa:4907.
UCSC	uc003pko.2. human.
Organism-specific databases
GeneCards	GC06P086216.
H-InvDB	HIX0032840.
HGNC	HGNC:8021. NT5E.
HPA	HPA017357.
MIM	129190. gene.
PharmGKB	PA31804.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P21589.
HOVERGEN	P21589.
OMA	P21589. RYDAMAL.
Enzyme and pathway databases
BRENDA	3.1.3.5. 247.
Pathway_Interaction_DB	hif1_tfpathway. HIF-1-alpha transcription factor network.
Reactome	REACT_1698. Metablism of nucleotides.
Gene expression databases
ArrayExpress	P21589.
Bgee	P21589.
CleanEx	HS_NT5E.
GermOnline	ENSG00000135318. Homo sapiens.
Family and domain databases
InterPro	IPR008334. 5'-Nucleotdase_C. IPR006146. 5'-Nucleotdase_CS. IPR006179. 5_nucleotidase. IPR004843. M-pesterase. [Graphical view]
Gene3D	G3DSA:3.90.780.10. 5'-Nucleotdase_C. 1 hit.
PANTHER	PTHR11575. 5_nucleotidase. 1 hit.
Pfam	PF02872. 5_nucleotid_C. 1 hit. PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR01607. APYRASEFAMLY.
PROSITE	PS00785. 5_NUCLEOTIDASE_1. 1 hit. PS00786. 5_NUCLEOTIDASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00806. Pentoxifylline.
NextBio	18883.
SOURCE	Search...

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Entry information

Entry name

5NTD_HUMAN

Accession

Primary (citable) accession number: P21589
Secondary accession number(s): O75520, Q5W116

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	July 7, 2009
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents