Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

5'-nucleotidase

Gene

Nt5e

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

Catalytic activityi

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi38Zinc 1By similarity1
Metal bindingi40Zinc 1By similarity1
Metal bindingi87Zinc 1By similarity1
Metal bindingi87Zinc 2By similarity1
Metal bindingi119Zinc 2By similarity1
Sitei120Transition state stabilizerBy similarity1
Sitei123Transition state stabilizerBy similarity1
Metal bindingi222Zinc 2By similarity1
Metal bindingi245Zinc 2By similarity1
Binding sitei247SubstrateBy similarity1
Binding sitei356SubstrateBy similarity1
Binding sitei392SubstrateBy similarity1
Binding sitei397SubstrateBy similarity1
Binding sitei419SubstrateBy similarity1

GO - Molecular functioni

  • 5'-nucleotidase activity Source: CACAO
  • ferrous iron binding Source: RGD
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  • adenosine metabolic process Source: RGD
  • AMP catabolic process Source: RGD
  • brain development Source: RGD
  • positive regulation of lipid biosynthetic process Source: RGD
  • purine nucleotide biosynthetic process Source: RGD
  • response to aluminum ion Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
5'-nucleotidase (EC:3.1.3.5)
Short name:
5'-NT
Alternative name(s):
Ecto-5'-nucleotidase
CD_antigen: CD73
Gene namesi
Name:Nt5e
Synonyms:Nt5, Nte
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61956. Nt5e.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • cell surface Source: RGD
  • integral component of membrane Source: RGD
  • plasma membrane Source: CACAO
  • synaptic membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075214.
GuidetoPHARMACOLOGYi1232.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Add BLAST28
ChainiPRO_000000001929 – 5515'-nucleotidaseAdd BLAST523
PropeptideiPRO_0000000020552 – 576Removed in mature formAdd BLAST25

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi53 ↔ 59By similarity
Glycosylationi55N-linked (GlcNAc...)Sequence analysis1
Glycosylationi313N-linked (GlcNAc...)Sequence analysis1
Glycosylationi335N-linked (GlcNAc...)Sequence analysis1
Glycosylationi349N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi355 ↔ 360By similarity
Disulfide bondi367 ↔ 389By similarity
Glycosylationi405N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi478 ↔ 481By similarity
Lipidationi551GPI-anchor amidated serine1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP21588.
PRIDEiP21588.

PTM databases

iPTMnetiP21588.
PhosphoSitePlusiP21588.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015057.

Chemistry databases

BindingDBiP21588.

Structurei

3D structure databases

ProteinModelPortaliP21588.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni502 – 508Substrate bindingBy similarity7

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4419. Eukaryota.
COG0737. LUCA.
HOGENOMiHOG000247215.
HOVERGENiHBG000026.
InParanoidiP21588.
PhylomeDBiP21588.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21588-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPAAATAPK WLLLALSALL PLWPTAKSWE LTIMHTNDVH SRLEQTSDDS
60 70 80 90 100
TKCLNASLCV GGVARLFTKV QQIRKEEPNV LLLDAGDQYQ GTIWFTVYKG
110 120 130 140 150
LEVAHFMNLL GYDAMALGNH EFDNGVEGLI DPLLRNVKFP ILSANIKARG
160 170 180 190 200
PLAPQISGLY LPYKVLSVGG EVVGIVGYTS KETPFLSNPG TNLVFEDEVT
210 220 230 240 250
ALQPEVDKLK TLNVNKIIAL GHSGFEMDKL IAQKVRGVDV VVGGHTNTFL
260 270 280 290 300
YTGNPPSKEV PAGKYPFIVT SDDGRKVPVV QAYAFGKYLG YLKVEFDDKG
310 320 330 340 350
NVVTSYGNPI LLNSTIREDA AIKADINQWR IKLDNYSTQE LGRTIVYLNG
360 370 380 390 400
SAQECRFREC NMGNLICDAM INNNLRHPDE MFWNHVSMCI VNGGGIRSPI
410 420 430 440 450
DERNNGTITW ENLAAVLPFG GTFDLVQLKG STLKKAFEHS VHRYGQSTGE
460 470 480 490 500
FLQVGGIHVV YDISRKPWDR VVQLKVLCTK CRVPIYEPLE MDKVYKVVLP
510 520 530 540 550
SYLVNGGDGF QMIKDELLKH DSGDQDISVV SEYISKMKVI YPAVEGRIKF
560 570
SAASHYQGSF PLIILSFWAV ILVLYQ
Length:576
Mass (Da):63,969
Last modified:May 1, 1991 - v1
Checksum:i9EB75DD51E678AA6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05214 mRNA. Translation: AAA40621.1.
PIRiA35036.
UniGeneiRn.40132.

Genome annotation databases

UCSCiRGD:61956. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05214 mRNA. Translation: AAA40621.1.
PIRiA35036.
UniGeneiRn.40132.

3D structure databases

ProteinModelPortaliP21588.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015057.

Chemistry databases

BindingDBiP21588.
ChEMBLiCHEMBL1075214.
GuidetoPHARMACOLOGYi1232.

PTM databases

iPTMnetiP21588.
PhosphoSitePlusiP21588.

Proteomic databases

PaxDbiP21588.
PRIDEiP21588.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:61956. rat.

Organism-specific databases

RGDi61956. Nt5e.

Phylogenomic databases

eggNOGiKOG4419. Eukaryota.
COG0737. LUCA.
HOGENOMiHOG000247215.
HOVERGENiHBG000026.
InParanoidiP21588.
PhylomeDBiP21588.

Miscellaneous databases

PROiP21588.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei5NTD_RAT
AccessioniPrimary (citable) accession number: P21588
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.