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Protein

Kit ligand

Gene

Kitlg

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Growth factor

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-RNO-1257604. PIP3 activates AKT signaling.
R-RNO-1433557. Signaling by SCF-KIT.
R-RNO-1433559. Regulation of KIT signaling.
R-RNO-5673001. RAF/MAP kinase cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Kit ligand
Alternative name(s):
Hematopoietic growth factor KL
Mast cell growth factor
Short name:
MGF
Stem cell factor
Short name:
SCF
c-Kit ligand
Cleaved into the following chain:
Soluble KIT ligand
Short name:
sKITLG
Gene namesi
Name:Kitlg
Synonyms:Kitl, Mgf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi3086. Kitlg.

Subcellular locationi

Isoform 2 :

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 214189ExtracellularSequence analysisAdd
BLAST
Transmembranei215 – 23723HelicalSequence analysisAdd
BLAST
Topological domaini238 – 27336CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25252 PublicationsAdd
BLAST
Chaini26 – 273248Kit ligandPRO_0000031917Add
BLAST
Chaini26 – 190165Soluble KIT ligandBy similarityPRO_0000403393Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei26 – 261Pyrrolidone carboxylic acid1 Publication
Disulfide bondi29 ↔ 1141 Publication
Disulfide bondi68 ↔ 1631 Publication
Glycosylationi90 – 901N-linked (GlcNAc...); partial1 Publication
Glycosylationi134 – 1341N-linked (GlcNAc...); partial1 Publication
Glycosylationi145 – 1451N-linked (GlcNAc...)1 Publication
Glycosylationi167 – 1671O-linked (GalNAc...)1 Publication
Glycosylationi168 – 1681O-linked (GalNAc...)1 Publication
Glycosylationi180 – 1801O-linked (GalNAc...)1 Publication
Glycosylationi195 – 1951N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.
The identity of N- and O-linked saccharides are not reported in PubMed:1708771. The O-linked polysaccharides are probably the mucin type linked to GalNAc.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei97 – 971Not glycosylated1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP21581.

PTM databases

PhosphoSiteiP21581.

Expressioni

Developmental stagei

Acts in the early stages of hematopoiesis.

Gene expression databases

ExpressionAtlasiP21581. baseline and differential.
GenevisibleiP21581. RN.

Interactioni

Subunit structurei

Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation.Curated

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008471.

Structurei

3D structure databases

ProteinModelPortaliP21581.
SMRiP21581. Positions 28-166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SCF family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IF3Y. Eukaryota.
ENOG410XYQR. LUCA.
GeneTreeiENSGT00390000018272.
HOGENOMiHOG000082493.
HOVERGENiHBG036146.
InParanoidiP21581.
KOiK05461.
PhylomeDBiP21581.
TreeFamiTF330811.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR003452. SCF.
[Graphical view]
PANTHERiPTHR11574. PTHR11574. 1 hit.
PfamiPF02404. SCF. 1 hit.
[Graphical view]
PIRSFiPIRSF015599. SCF. 1 hit.
SUPFAMiSSF47266. SSF47266. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P21581-1) [UniParc]FASTAAdd to basket

Also known as: KL-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKKTQTWIIT CIYLQLLLFN PLVKTQEICR NPVTDNVKDI TKLVANLPND
60 70 80 90 100
YMITLNYVAG MDVLPSHCWL RDMVTHLSVS LTTLLDKFSN ISEGLSNYSI
110 120 130 140 150
IDKLGKIVDD LVACMEENAP KNVKESLKKP ETRNFTPEEF FSIFNRSIDA
160 170 180 190 200
FKDFMVASDT SDCVLSSTLG PEKDSRVSVT KPFMLPPVAA SSLRNDSSSS
210 220 230 240 250
NRKAAKSPED PGLQWTAMAL PALISLVIGF AFGALYWKKK QSSLTRAVEN
260 270
IQINEEDNEI SMLQQKEREF QEV
Length:273
Mass (Da):30,712
Last modified:August 30, 2002 - v2
Checksum:iC0F56527DC93FD27
GO
Isoform 2 (identifier: P21581-2) [UniParc]FASTAAdd to basket

Also known as: KL-2

The sequence of this isoform differs from the canonical sequence as follows:
     174-202: DSRVSVTKPFMLPPVAASSLRNDSSSSNR → G

Show »
Length:245
Mass (Da):27,681
Checksum:i9615130876AC9D52
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti207 – 2071S → P in AAD02828 (Ref. 1) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei174 – 20229DSRVS…SSSNR → G in isoform 2. 1 PublicationVSP_006025Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071204 mRNA. Translation: AAD02827.1.
AF071205 mRNA. Translation: AAD02828.1.
M59966 mRNA. Translation: AAA42117.1.
PIRiB35974.
RefSeqiNP_068615.1. NM_021843.4. [P21581-1]
UniGeneiRn.44216.

Genome annotation databases

EnsembliENSRNOT00000007231; ENSRNOP00000007231; ENSRNOG00000005386. [P21581-2]
ENSRNOT00000008471; ENSRNOP00000008471; ENSRNOG00000005386. [P21581-1]
GeneIDi60427.
KEGGirno:60427.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071204 mRNA. Translation: AAD02827.1.
AF071205 mRNA. Translation: AAD02828.1.
M59966 mRNA. Translation: AAA42117.1.
PIRiB35974.
RefSeqiNP_068615.1. NM_021843.4. [P21581-1]
UniGeneiRn.44216.

3D structure databases

ProteinModelPortaliP21581.
SMRiP21581. Positions 28-166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008471.

PTM databases

PhosphoSiteiP21581.

Proteomic databases

PaxDbiP21581.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007231; ENSRNOP00000007231; ENSRNOG00000005386. [P21581-2]
ENSRNOT00000008471; ENSRNOP00000008471; ENSRNOG00000005386. [P21581-1]
GeneIDi60427.
KEGGirno:60427.

Organism-specific databases

CTDi4254.
RGDi3086. Kitlg.

Phylogenomic databases

eggNOGiENOG410IF3Y. Eukaryota.
ENOG410XYQR. LUCA.
GeneTreeiENSGT00390000018272.
HOGENOMiHOG000082493.
HOVERGENiHBG036146.
InParanoidiP21581.
KOiK05461.
PhylomeDBiP21581.
TreeFamiTF330811.

Enzyme and pathway databases

ReactomeiR-RNO-1257604. PIP3 activates AKT signaling.
R-RNO-1433557. Signaling by SCF-KIT.
R-RNO-1433559. Regulation of KIT signaling.
R-RNO-5673001. RAF/MAP kinase cascade.

Miscellaneous databases

NextBioi612146.
PROiP21581.

Gene expression databases

ExpressionAtlasiP21581. baseline and differential.
GenevisibleiP21581. RN.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR003452. SCF.
[Graphical view]
PANTHERiPTHR11574. PTHR11574. 1 hit.
PfamiPF02404. SCF. 1 hit.
[Graphical view]
PIRSFiPIRSF015599. SCF. 1 hit.
SUPFAMiSSF47266. SSF47266. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Teramoto T., Nagashima M., Thorgeirsson S.S.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-201, PARTIAL PROTEIN SEQUENCE.
  3. "Amino acid sequence and post-translational modification of stem cell factor isolated from buffalo rat liver cell-conditioned medium."
    Lu H.S., Clogston C.L., Wypych J., Fausset P.R., Lauren S., Mendiaz E.A., Zsebo K.M., Langley K.E.
    J. Biol. Chem. 266:8102-8107(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-190, PYROGLUTAMATE FORMATION AT GLN-26, GLYCOSYLATION AT ASN-90; ASN-134; ASN-145; SER-167; THR-168; THR-180 AND ASN-195, ABSENCE OF GLYCOSYLATION AT ASN-97, DISULFIDE BONDS.
    Strain: Buffalo.
    Tissue: Liver.
  4. "Identification, purification, and biological characterization of hematopoietic stem cell factor from buffalo rat liver-conditioned medium."
    Zsebo K.M., Wypych J., McNiece I.K., Lu H.S., Smith K.A., Karkare S.B., Sachdev R.K., Yuschenkoff V.N., Birkett N.C., Williams L.R., Satyagal V.N., Tung W., Bosselman R.A., Mendiaz E.A., Langley K.E.
    Cell 63:195-201(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-39.

Entry informationi

Entry nameiSCF_RAT
AccessioniPrimary (citable) accession number: P21581
Secondary accession number(s): Q9QWZ4, Q9Z2E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: August 30, 2002
Last modified: February 17, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.