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Protein

Vacuolar protein sorting-associated protein 1

Gene

VPS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for protein sorting in meiotic cell division of Saccharomyces cerevisiae; it binds microtubules. Could also be involved in microtubule-associated motility. Necessary for membrane protein retention in a late Golgi compartment. Interacts with the MVP1 protein.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 43GTPSequence analysis8
Nucleotide bindingi178 – 182GTPSequence analysis5
Nucleotide bindingi247 – 250GTPSequence analysis4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW
  • microtubule binding Source: GO_Central

GO - Biological processi

  • actin cytoskeleton organization Source: SGD
  • cell division Source: UniProtKB-KW
  • dynamin family protein polymerization involved in mitochondrial fission Source: GO_Central
  • endocytosis Source: SGD
  • lipid tube assembly Source: SGD
  • meiotic cell cycle Source: UniProtKB-KW
  • membrane fusion Source: GO_Central
  • mitochondrial fission Source: GO_Central
  • peroxisome fission Source: SGD
  • peroxisome organization Source: SGD
  • protein retention in Golgi apparatus Source: SGD
  • protein targeting to vacuole Source: SGD
  • vacuolar transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell cycle, Cell division, Meiosis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31979-MONOMER.

Protein family/group databases

TCDBi9.A.63.1.1. the retromer-dependnet vacuolar protein sorting (r-vps) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 1
Gene namesi
Name:VPS1
Synonyms:LAM1, SPO15
Ordered Locus Names:YKR001C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR001C.
SGDiS000001709. VPS1.

Subcellular locationi

  • Cytoplasmcytoskeleton 1 Publication

GO - Cellular componenti

  • actin cortical patch Source: SGD
  • fungal-type vacuole membrane Source: SGD
  • late endosome Source: SGD
  • microtubule Source: UniProtKB-KW
  • peroxisome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002065881 – 704Vacuolar protein sorting-associated protein 1Add BLAST704

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei579PhosphoserineCombined sources1
Modified residuei599PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP21576.
PRIDEiP21576.
TopDownProteomicsiP21576.

PTM databases

iPTMnetiP21576.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi34133. 468 interactors.
DIPiDIP-6290N.
IntActiP21576. 59 interactors.
MINTiMINT-646911.

Structurei

3D structure databases

ProteinModelPortaliP21576.
SMRiP21576.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 336Dynamin-type GAdd BLAST311
Domaini618 – 704GEDPROSITE-ProRule annotationAdd BLAST87

Sequence similaritiesi

Contains 1 GED domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000119213.
HOGENOMiHOG000161068.
InParanoidiP21576.
KOiK17065.
OMAiINGSRAM.
OrthoDBiEOG092C16PN.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR003130. GED.
IPR020850. GED_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 2 hits.
PfamiPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21576-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEHLISTIN KLQDALAPLG GGSQSPIDLP QITVVGSQSS GKSSVLENIV
60 70 80 90 100
GRDFLPRGTG IVTRRPLVLQ LINRRPKKSE HAKVNQTANE LIDLNINDDD
110 120 130 140 150
KKKDESGKHQ NEGQSEDNKE EWGEFLHLPG KKFYNFDEIR KEIVKETDKV
160 170 180 190 200
TGANSGISSV PINLRIYSPH VLTLTLVDLP GLTKVPVGDQ PPDIERQIKD
210 220 230 240 250
MLLKYISKPN AIILSVNAAN TDLANSDGLK LAREVDPEGT RTIGVLTKVD
260 270 280 290 300
LMDQGTDVID ILAGRVIPLR YGYIPVINRG QKDIEHKKTI REALENERKF
310 320 330 340 350
FENHPSYSSK AHYCGTPYLA KKLNSILLHH IRQTLPEIKA KIEATLKKYQ
360 370 380 390 400
NELINLGPET MDSASSVVLS MITDFSNEYA GILDGEAKEL SSQELSGGAR
410 420 430 440 450
ISYVFHETFK NGVDSLDPFD QIKDSDIRTI MYNSSGSAPS LFVGTEAFEV
460 470 480 490 500
LVKQQIRRFE EPSLRLVTLV FDELVRMLKQ IISQPKYSRY PALREAISNQ
510 520 530 540 550
FIQFLKDATI PTNEFVVDII KAEQTYINTA HPDLLKGSQA MVMVEEKLHP
560 570 580 590 600
RQVAVDPKTG KPLPTQPSSS KAPVMEEKSG FFGGFFSTKN KKKLAALESP
610 620 630 640 650
PPVLKATGQM TERETMETEV IKLLISSYFS IVKRTIADII PKALMLKLIV
660 670 680 690 700
KSKTDIQKVL LEKLYGKQDI EELTKENDIT IQRRKECKKM VEILRNASQI

VSSV
Length:704
Mass (Da):78,737
Last modified:July 1, 1993 - v2
Checksum:iD9AB527C04B3A977
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33T → N in AAA35216 (PubMed:2112425).Curated1
Sequence conflicti111N → E in AAA35216 (PubMed:2112425).Curated1
Sequence conflicti141K → Q in CAA38214 (PubMed:1825352).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54316 Genomic DNA. Translation: CAA38214.1.
M33315 Genomic DNA. Translation: AAA35216.1.
X65124 Genomic DNA. Translation: CAA46251.1.
Z28226 Genomic DNA. Translation: CAA82071.1.
BK006944 Genomic DNA. Translation: DAA09157.1.
PIRiS25820.
RefSeqiNP_012926.1. NM_001179791.1.

Genome annotation databases

EnsemblFungiiYKR001C; YKR001C; YKR001C.
GeneIDi853870.
KEGGisce:YKR001C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54316 Genomic DNA. Translation: CAA38214.1.
M33315 Genomic DNA. Translation: AAA35216.1.
X65124 Genomic DNA. Translation: CAA46251.1.
Z28226 Genomic DNA. Translation: CAA82071.1.
BK006944 Genomic DNA. Translation: DAA09157.1.
PIRiS25820.
RefSeqiNP_012926.1. NM_001179791.1.

3D structure databases

ProteinModelPortaliP21576.
SMRiP21576.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34133. 468 interactors.
DIPiDIP-6290N.
IntActiP21576. 59 interactors.
MINTiMINT-646911.

Protein family/group databases

TCDBi9.A.63.1.1. the retromer-dependnet vacuolar protein sorting (r-vps) family.

PTM databases

iPTMnetiP21576.

Proteomic databases

MaxQBiP21576.
PRIDEiP21576.
TopDownProteomicsiP21576.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR001C; YKR001C; YKR001C.
GeneIDi853870.
KEGGisce:YKR001C.

Organism-specific databases

EuPathDBiFungiDB:YKR001C.
SGDiS000001709. VPS1.

Phylogenomic databases

GeneTreeiENSGT00760000119213.
HOGENOMiHOG000161068.
InParanoidiP21576.
KOiK17065.
OMAiINGSRAM.
OrthoDBiEOG092C16PN.

Enzyme and pathway databases

BioCyciYEAST:G3O-31979-MONOMER.

Miscellaneous databases

PROiP21576.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR003130. GED.
IPR020850. GED_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 2 hits.
PfamiPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVPS1_YEAST
AccessioniPrimary (citable) accession number: P21576
Secondary accession number(s): D6VXT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5960 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.