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P21575 (DYN1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dynamin-1
EC=3.6.5.5
Alternative name(s):
B-dynamin
D100
Dynamin, brain
Gene names
Name:Dnm1
Synonyms:Dnm
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length864 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis By similarity.

Catalytic activity

GTP + H2O = GDP + phosphate.

Subunit structure

Interacts with CAV1 and SH3GLB1. Interacts with SNX9 By similarity. Interacts with PHOCN By similarity. Binds SH3GL1, SH3GL2 and SH3GL3. Interacts with MYO1E (via SH3 domain). Ref.6 Ref.7 Ref.10

Subcellular location

Cytoplasm Ref.8. Cytoplasm › cytoskeleton. Note: Microtubule-associated. Isoform-specific localization. Ref.8

Isoform 2: Cytoplasm Ref.8.

Isoform 6: Cytoplasm Ref.8. Golgi apparatus Ref.8.

Tissue specificity

Brain-specific (peripheral sensory neurons). Ref.8

Developmental stage

Expressed in neurons after maturation.

Post-translational modification

Constitutively phosphorylated by CDK5 in nerve terminals and dephosphorylated by calcineurin when synaptic vesicle endocytosis (SVE) is stimulated by depolarization-dependent calcium influx. Ref.3 Ref.9 Ref.11

Sequence similarities

Belongs to the dynamin family.

Contains 1 GED domain.

Contains 1 PH domain.

Caution

Absence of tyrosine and threonine phosphorylation is demonstrated in Ref.3. However, tyrosine and threonine phosphorylation may still occur in different experimental conditions.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Bin1O088392EBI-80070,EBI-80095
Sh3gl2O351792EBI-80070,EBI-1149197
Sh3gl3O351802EBI-80070,EBI-1149266

Alternative products

This entry describes 8 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P21575-1)

Also known as: aa;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P21575-2)

Also known as: ab;

The sequence of this isoform differs from the canonical sequence as follows:
     845-864: SRSGQASPSRPESPRPPFDL → RITISDP
Note: Localized to numerous punctate spots of various sizes distributed along the plasma membrane and throughout the cytoplasm.
Isoform 3 (identifier: P21575-3)

Also known as: ac;

The sequence of this isoform differs from the canonical sequence as follows:
     766-814: QSVPAGRRSP...AGSALGGAPP → AHVQPHAAAP...PRALQAGGFP
     815-864: Missing.
Isoform 4 (identifier: P21575-4)

Also known as: ad;

The sequence of this isoform differs from the canonical sequence as follows:
     845-864: SRSGQASPSRPESPRPPFDL → SQPIGSGKSVPS
Isoform 5 (identifier: P21575-5)

Also known as: ba;

The sequence of this isoform differs from the canonical sequence as follows:
     407-444: LAFEATVKKQ...LTSTIRKCSE → MAFETIVKKQ...LISTVRQCTK
Isoform 6 (identifier: P21575-6)

Also known as: bb;

The sequence of this isoform differs from the canonical sequence as follows:
     407-444: LAFEATVKKQ...LTSTIRKCSE → MAFETIVKKQ...LISTVRQCTK
     845-864: SRSGQASPSRPESPRPPFDL → RITISDP
Note: Diffuse cytoplasmic distribution with some localization to the Golgi apparatus.
Isoform 7 (identifier: P21575-7)

Also known as: bc;

The sequence of this isoform differs from the canonical sequence as follows:
     407-444: LAFEATVKKQ...LTSTIRKCSE → MAFETIVKKQ...LISTVRQCTK
     766-814: QSVPAGRRSP...AGSALGGAPP → AHVQPHAAAP...PRALQAGGFP
     815-864: Missing.
Isoform 8 (identifier: P21575-8)

Also known as: bd;

The sequence of this isoform differs from the canonical sequence as follows:
     407-444: LAFEATVKKQ...LTSTIRKCSE → MAFETIVKKQ...LISTVRQCTK
     845-864: SRSGQASPSRPESPRPPFDL → SQPIGSGKSVPS

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 864864Dynamin-1
PRO_0000206565

Regions

Domain519 – 625107PH
Domain659 – 75092GED
Nucleotide binding38 – 458GTP Potential
Nucleotide binding136 – 1405GTP Potential
Nucleotide binding205 – 2084GTP Potential
Compositional bias753 – 85199Pro-rich

Amino acid modifications

Modified residue801Phosphotyrosine By similarity
Modified residue1251Phosphotyrosine By similarity
Modified residue3471Phosphoserine Ref.3
Modified residue3541Phosphotyrosine By similarity
Modified residue5121Phosphoserine Ref.3
Modified residue7781Phosphoserine By similarity
Modified residue8221Phosphoserine Ref.3
Modified residue8511Phosphoserine Ref.3
Modified residue8571Phosphoserine Ref.3

Natural variations

Alternative sequence407 – 44438LAFEA…RKCSE → MAFETIVKKQVKKIREPCLK CVDMVISELISTVRQCTK in isoform 5, isoform 6, isoform 7 and isoform 8.
VSP_034033
Alternative sequence766 – 81449QSVPA…GGAPP → AHVQPHAAAPSPRRAPSPAR VAGPCSWASACWIRPGGGSP RALQAGGFP in isoform 3 and isoform 7.
VSP_034034
Alternative sequence815 – 86450Missing in isoform 3 and isoform 7.
VSP_034035
Alternative sequence845 – 86420SRSGQ…PPFDL → RITISDP in isoform 2 and isoform 6.
VSP_034037
Alternative sequence845 – 86420SRSGQ…PPFDL → SQPIGSGKSVPS in isoform 4 and isoform 8.
VSP_034036

Secondary structure

1 ............................................................................................. 864
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (aa) [UniParc].

Last modified June 10, 2008. Version 2.
Checksum: 02EC9B533CC2EC83

FASTA86497,295
        10         20         30         40         50         60 
MGNRGMEDLI PLVNRLQDAF SAIGQNADLD LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 

        70         80         90        100        110        120 
SGIVTRRPLV LQLVNSTTEY AEFLHCKGKK FTDFEEVRLE IEAETDRVTG TNKGISPVPI 

       130        140        150        160        170        180 
NLRVYSPHVL NLTLVDLPGM TKVPVGDQPP DIEFQIRDML MQFVTKENCL ILAVSPANSD 

       190        200        210        220        230        240 
LANSDALKIA KEVDPQGQRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK 

       250        260        270        280        290        300 
DIDGKKDITA ALAAERKFFL SHPSYRHLAD RMGTPYLQKV LNQQLTNHIR DTLPGLRNKL 

       310        320        330        340        350        360 
QSQLLSIEKE VDEYKNFRPD DPARKTKALL QMVQQFAVDF EKRIEGSGDQ IDTYELSGGA 

       370        380        390        400        410        420 
RINRIFHERF PFELVKMEFD EKELRREISY AIKNIHGIRT GLFTPDLAFE ATVKKQVQKL 

       430        440        450        460        470        480 
KEPSIKCVDM VVSELTSTIR KCSEKLQQYP RLREEMERIV TTHIREREGR TKEQVMLLID 

       490        500        510        520        530        540 
IELAYMNTNH EDFIGFANAQ QRSNQMNKKK TSGNQDEILV IRKGWLTINN IGIMKGGSKE 

       550        560        570        580        590        600 
YWFVLTAENL SWYKDDEEKE KKYMLSVDNL KLRDVEKGFM SSKHIFALFN TEQRNVYKDY 

       610        620        630        640        650        660 
RQLELACETQ EEVDSWKASF LRAGVYPERV GDKEKASETE ENGSDSFMHS MDPQLERQVE 

       670        680        690        700        710        720 
TIRNLVDSYM AIVNKTVRDL MPKTIMHLMI NNTKEFIFSE LLANLYSCGD QNTLMEESAE 

       730        740        750        760        770        780 
QAQRRDEMLR MYHALKEALS IIGDINTTTV STPMPPPVDD SWLQVQSVPA GRRSPTSSPT 

       790        800        810        820        830        840 
PQRRAPAVPP ARPGSRGPAP GPPPAGSALG GAPPVPSRPG ASPDPFGPPP QVPSRPNRAP 

       850        860 
PGVPSRSGQA SPSRPESPRP PFDL

« Hide

Isoform 2 (ab) [UniParc].

Checksum: 5D9294C5D1015A88
Show »

FASTA85195,927
Isoform 3 (ac) [UniParc].

Checksum: A36766D15D5AC466
Show »

FASTA81492,376
Isoform 4 (ad) [UniParc].

Checksum: 727CC965C3929D99
Show »

FASTA85696,270
Isoform 5 (ba) [UniParc].

Checksum: 4E27474E413CCEE7
Show »

FASTA86497,424
Isoform 6 (bb) [UniParc].

Checksum: 5FE0938290E39F51
Show »

FASTA85196,057
Isoform 7 (bc) [UniParc].

Checksum: 686364B0BFE8865C
Show »

FASTA81492,505
Isoform 8 (bd) [UniParc].

Checksum: 1A01F8B610A0EF9E
Show »

FASTA85696,399

References

« Hide 'large scale' references
[1]"Molecular cloning of the microtubule-associated mechanochemical enzyme dynamin reveals homology with a new family of GTP-binding proteins."
Obar R.A., Collins C.A., Hammarback J.A., Shpetner H.S., Vallee R.B.
Nature 347:256-261(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: Sprague-Dawley.
Tissue: Brain.
[2]Lubec G., Afjehi-Sadat L., Diao W.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 5-15; 45-54; 67-87; 91-107; 116-157; 167-188; 257-266; 300-315; 328-342; 400-415; 511-522 AND 584-594 (ISOFORM 1/2/3/4), MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus and Spinal cord.
[3]"The in vivo phosphorylation sites of rat brain dynamin I."
Graham M.E., Anggono V., Bache N., Larsen M.R., Craft G.E., Robinson P.J.
J. Biol. Chem. 282:14695-14707(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 343-364; 511-522 AND 797-838 (ISOFORM 1/2/4/5/6/8), PROTEIN SEQUENCE OF 847-864 (ISOFORM 1/5), PHOSPHORYLATION AT SER-347; SER-512; SER-774; SER-778; SER-822; SER-851 AND SER-857, MASS SPECTROMETRY.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 368-581 (ISOFORM 5/6/7/8).
Tissue: Brain.
[5]"Amgen rat EST program."
Amgen EST program
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 668-864 (ISOFORM 1/5).
Tissue: Brain and Hypothalamus.
[6]"Myosin 1E interacts with synaptojanin-1 and dynamin and is involved in endocytosis."
Krendel M., Osterweil E.K., Mooseker M.S.
FEBS Lett. 581:644-650(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MYO1E.
[7]"The SH3p4/Sh3p8/SH3p13 protein family: binding partners for synaptojanin and dynamin via a Grb2-like Src homology 3 domain."
Ringstad N., Nemoto Y., De Camilli P.
Proc. Natl. Acad. Sci. U.S.A. 94:8569-8574(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SH3GL1; SH3GL2 AND SH3GL3.
Tissue: Brain.
[8]"Differential distribution of dynamin isoforms in mammalian cells."
Cao H., Garcia F., McNiven M.A.
Mol. Biol. Cell 9:2595-2609(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[9]"The dephosphins: dephosphorylation by calcineurin triggers synaptic vesicle endocytosis."
Cousin M.A., Robinson P.J.
Trends Neurosci. 24:659-665(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: ROLE OF PHOSPHORYLATION.
[10]"Interactions of phocein with nucleoside-diphosphate kinase, Eps15, and Dynamin I."
Baillat G., Gaillard S., Castets F., Monneron A.
J. Biol. Chem. 277:18961-18966(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PHOCN.
[11]"Cdk5 is essential for synaptic vesicle endocytosis."
Tan T.C., Valova V.A., Malladi C.S., Graham M.E., Berven L.A., Jupp O.J., Hansra G., McClure S.J., Sarcevic B., Boadle R.A., Larsen M.R., Cousin M.A., Robinson P.J.
Nat. Cell Biol. 5:701-710(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY CDK5.
[12]"Dynamin I phosphorylation and the control of synaptic vesicle endocytosis."
Smillie K.J., Cousin M.A.
Biochem. Soc. Symp. 72:87-97(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW ON FUNCTION.
[13]"Crystal structure of the GTPase domain of rat dynamin 1."
Reubold T.F., Eschenburg S., Becker A., Leonard M., Schmid S.L., Vallee R.B., Kull F.J., Manstein D.J.
Proc. Natl. Acad. Sci. U.S.A. 102:13093-13098(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 6-304.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X54531 mRNA. Translation: CAA38397.1.
CO398357 mRNA. No translation available.
CB583951 mRNA. No translation available.
CB708564 mRNA. No translation available.
IPIIPI00558839.
IPI00782657.
IPI00896118.
IPI00896147.
IPI00896174.
IPI00896199.
IPI00896219.
IPI00952286.
PIRS11508.
RefSeqNP_542420.1. NM_080689.4.
UniGeneRn.10354.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2AKAX-ray1.90B6-304[»]
3ZVRX-ray3.10A1-752[»]
ProteinModelPortalP21575.
SMRP21575. Positions 6-304, 518-630.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-30978N.
IntActP21575. 7 interactions.
MINTMINT-95899.
STRING10116.ENSRNOP00000046016.

PTM databases

PhosphoSiteP21575.

2D gel databases

World-2DPAGE0004:P21575.

Proteomic databases

PaxDbP21575.
PRIDEP21575.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000042762; ENSRNOP00000047444; ENSRNOG00000033835.
ENSRNOT00000064039; ENSRNOP00000060845; ENSRNOG00000033835.
GeneID140694.
KEGGrno:140694.
UCSCRGD:71096. rat.

Organism-specific databases

CTD1759.
RGD71096. Dnm1.

Phylogenomic databases

eggNOGCOG0699.
GeneTreeENSGT00690000101762.
HOGENOMHOG000161069.
HOVERGENHBG107833.
KOK01528.

Enzyme and pathway databases

BRENDA3.6.5.5. 5301.
ReactomeREACT_111984. Signal Transduction.

Gene expression databases

ArrayExpressP21575.
GenevestigatorP21575.
GermOnlineENSRNOG00000033835. Rattus norvegicus.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR022812. Dynamin.
IPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR003130. GED.
IPR020850. GTPase_effector_domain_GED.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
[Graphical view]
PANTHERPTHR11566. PTHR11566. 1 hit.
PfamPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
PRINTSPR00195. DYNAMIN.
SMARTSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
PROSITEPS00410. DYNAMIN. 1 hit.
PS51388. GED. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBP21575.
ChEMBLCHEMBL1075191.
EvolutionaryTraceP21575.
NextBio620679.
PMAP-CutDBP21575.

Entry information

Entry nameDYN1_RAT
AccessionPrimary (citable) accession number: P21575
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: June 10, 2008
Last modified: April 3, 2013
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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