Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dynamin-1

Gene

Dnm1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis (By similarity).By similarity

Caution

Absence of tyrosine and threonine phosphorylation is demonstrated in PubMed:17376771. However, tyrosine and threonine phosphorylation may still occur in different experimental conditions.Curated

Catalytic activityi

GTP + H2O = GDP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 45GTPSequence analysis8
Nucleotide bindingi136 – 140GTPSequence analysis5
Nucleotide bindingi205 – 208GTPSequence analysis4

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • microtubule binding Source: GO_Central
  • protein-containing complex binding Source: RGD
  • protein dimerization activity Source: Ensembl
  • protein kinase binding Source: Ensembl
  • SH3 domain binding Source: RGD

GO - Biological processi

  • adult locomotory behavior Source: Ensembl
  • clathrin-dependent endocytosis Source: InterPro
  • dynamin family protein polymerization involved in mitochondrial fission Source: GO_Central
  • endosome organization Source: Ensembl
  • G-protein coupled receptor internalization Source: RGD
  • membrane fusion Source: GO_Central
  • mitochondrial fission Source: GO_Central
  • positive regulation of synaptic vesicle endocytosis Source: RGD
  • positive regulation of synaptic vesicle recycling Source: RGD
  • protein tetramerization Source: Ensembl
  • receptor internalization Source: UniProtKB
  • receptor-mediated endocytosis Source: RGD
  • regulation of synaptic vesicle endocytosis Source: RGD
  • response to amyloid-beta Source: RGD
  • sensory perception of sound Source: Ensembl
  • synaptic transmission, GABAergic Source: Ensembl
  • synaptic vesicle budding from presynaptic endocytic zone membrane Source: Ensembl
  • toxin transport Source: Ensembl

Keywordsi

Molecular functionHydrolase, Motor protein
Biological processEndocytosis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.5.5 5301
ReactomeiR-RNO-190873 Gap junction degradation
R-RNO-196025 Formation of annular gap junctions
R-RNO-2132295 MHC class II antigen presentation
R-RNO-437239 Recycling pathway of L1
R-RNO-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Dynamin-1 (EC:3.6.5.5)
Alternative name(s):
B-dynamin
D100
Dynamin, brain
Gene namesi
Name:Dnm1
Synonyms:Dnm
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi71096 Dnm1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus, Microtubule

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075191

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002065651 – 864Dynamin-1Add BLAST864

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei80PhosphotyrosineBy similarity1
Modified residuei125Nitrated tyrosine; alternateBy similarity1
Modified residuei125Phosphotyrosine; alternateBy similarity1
Modified residuei306PhosphoserineBy similarity1
Modified residuei347PhosphoserineCombined sources1 Publication1
Modified residuei354PhosphotyrosineBy similarity1
Modified residuei512Phosphoserine1 Publication1
Modified residuei774Phosphoserine1 Publication1
Modified residuei778Phosphoserine1 Publication1
Modified residuei796Omega-N-methylarginineBy similarity1
Modified residuei822Phosphoserine1 Publication1
Modified residuei851PhosphoserineCombined sources1 Publication1
Modified residuei857PhosphoserineCombined sources1 Publication1

Post-translational modificationi

Constitutively phosphorylated by CDK5 in nerve terminals and dephosphorylated by calcineurin when synaptic vesicle endocytosis (SVE) is stimulated by depolarization-dependent calcium influx.2 Publications

Keywords - PTMi

Methylation, Nitration, Phosphoprotein

Proteomic databases

PaxDbiP21575
PRIDEiP21575

2D gel databases

World-2DPAGEi0004:P21575

PTM databases

iPTMnetiP21575
PhosphoSitePlusiP21575

Miscellaneous databases

PMAP-CutDBiP21575

Expressioni

Tissue specificityi

Brain-specific (peripheral sensory neurons).1 Publication

Developmental stagei

Expressed in neurons after maturation.

Gene expression databases

BgeeiENSRNOG00000033835
GenevisibleiP21575 RN

Interactioni

Subunit structurei

Interacts with CAV1 and SH3GLB1. Interacts with SNX9. Interacts with SNX33 (via SH3 domain) (By similarity). Interacts with PHOCN. Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity). Binds SH3GL1, SH3GL2 and SH3GL3. Interacts with MYO1E (via SH3 domain). Interacts with UNC119; leading to a decrease of DNM1 GTPase activity (By similarity). Interacts with DIAPH1 (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • microtubule binding Source: GO_Central
  • protein dimerization activity Source: Ensembl
  • protein kinase binding Source: Ensembl
  • SH3 domain binding Source: RGD

Protein-protein interaction databases

BioGridi250837, 22 interactors
CORUMiP21575
DIPiDIP-30978N
IntActiP21575, 17 interactors
MINTiP21575
STRINGi10116.ENSRNOP00000047444

Chemistry databases

BindingDBiP21575

Structurei

Secondary structure

1864
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 8Combined sources3
Helixi9 – 20Combined sources12
Turni22 – 24Combined sources3
Helixi27 – 29Combined sources3
Beta strandi33 – 39Combined sources7
Turni40 – 42Combined sources3
Helixi44 – 52Combined sources9
Beta strandi60 – 62Combined sources3
Beta strandi69 – 75Combined sources7
Beta strandi80 – 83Combined sources4
Turni85 – 88Combined sources4
Helixi94 – 108Combined sources15
Beta strandi120 – 126Combined sources7
Beta strandi131 – 136Combined sources6
Beta strandi146 – 148Combined sources3
Helixi152 – 164Combined sources13
Beta strandi169 – 179Combined sources11
Helixi181 – 183Combined sources3
Helixi185 – 193Combined sources9
Beta strandi198 – 205Combined sources8
Helixi207 – 209Combined sources3
Helixi217 – 220Combined sources4
Beta strandi231 – 233Combined sources3
Beta strandi240 – 247Combined sources8
Helixi248 – 261Combined sources14
Turni263 – 265Combined sources3
Helixi266 – 271Combined sources6
Helixi274 – 303Combined sources30
Beta strandi321 – 323Combined sources3
Helixi326 – 344Combined sources19
Helixi358 – 367Combined sources10
Helixi369 – 375Combined sources7
Helixi381 – 394Combined sources14
Helixi404 – 418Combined sources15
Helixi421 – 442Combined sources22
Helixi443 – 446Combined sources4
Helixi450 – 483Combined sources34
Beta strandi520 – 531Combined sources12
Beta strandi540 – 555Combined sources16
Beta strandi561 – 566Combined sources6
Beta strandi570 – 575Combined sources6
Beta strandi584 – 590Combined sources7
Beta strandi600 – 609Combined sources10
Helixi610 – 622Combined sources13
Helixi654 – 688Combined sources35
Helixi690 – 699Combined sources10
Helixi701 – 707Combined sources7
Turni711 – 715Combined sources5
Helixi719 – 742Combined sources24

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AKAX-ray1.90B6-304[»]
3ZVRX-ray3.10A1-752[»]
ProteinModelPortaliP21575
SMRiP21575
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21575

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 294Dynamin-type GPROSITE-ProRule annotationAdd BLAST267
Domaini519 – 625PHPROSITE-ProRule annotationAdd BLAST107
Domaini659 – 750GEDPROSITE-ProRule annotationAdd BLAST92

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni38 – 45G1 motifPROSITE-ProRule annotation8
Regioni64 – 66G2 motifPROSITE-ProRule annotation3
Regioni136 – 139G3 motifPROSITE-ProRule annotation4
Regioni205 – 208G4 motifPROSITE-ProRule annotation4
Regioni235 – 238G5 motifPROSITE-ProRule annotation4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi753 – 851Pro-richAdd BLAST99

Sequence similaritiesi

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0446 Eukaryota
COG0699 LUCA
GeneTreeiENSGT00760000119213
HOGENOMiHOG000161069
HOVERGENiHBG107833
InParanoidiP21575
KOiK01528
OMAiKIKEPCL
PhylomeDBiP21575

Family and domain databases

CDDicd08771 DLP_1, 1 hit
Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR027741 DNM1
IPR000375 Dynamin_central
IPR001401 Dynamin_GTPase
IPR019762 Dynamin_GTPase_CS
IPR022812 Dynamin_SF
IPR030381 G_DYNAMIN_dom
IPR003130 GED
IPR020850 GED_dom
IPR027417 P-loop_NTPase
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PANTHERiPTHR11566 PTHR11566, 1 hit
PTHR11566:SF32 PTHR11566:SF32, 1 hit
PfamiView protein in Pfam
PF01031 Dynamin_M, 1 hit
PF00350 Dynamin_N, 1 hit
PF02212 GED, 1 hit
PF00169 PH, 1 hit
PRINTSiPR00195 DYNAMIN
SMARTiView protein in SMART
SM00053 DYNc, 1 hit
SM00302 GED, 1 hit
SM00233 PH, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00410 G_DYNAMIN_1, 1 hit
PS51718 G_DYNAMIN_2, 1 hit
PS51388 GED, 1 hit
PS50003 PH_DOMAIN, 1 hit

Sequences (8)i

Sequence statusi: Complete.

This entry describes 8 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P21575-1) [UniParc]FASTAAdd to basket
Also known as: aa

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNRGMEDLI PLVNRLQDAF SAIGQNADLD LPQIAVVGGQ SAGKSSVLEN
60 70 80 90 100
FVGRDFLPRG SGIVTRRPLV LQLVNSTTEY AEFLHCKGKK FTDFEEVRLE
110 120 130 140 150
IEAETDRVTG TNKGISPVPI NLRVYSPHVL NLTLVDLPGM TKVPVGDQPP
160 170 180 190 200
DIEFQIRDML MQFVTKENCL ILAVSPANSD LANSDALKIA KEVDPQGQRT
210 220 230 240 250
IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK DIDGKKDITA
260 270 280 290 300
ALAAERKFFL SHPSYRHLAD RMGTPYLQKV LNQQLTNHIR DTLPGLRNKL
310 320 330 340 350
QSQLLSIEKE VDEYKNFRPD DPARKTKALL QMVQQFAVDF EKRIEGSGDQ
360 370 380 390 400
IDTYELSGGA RINRIFHERF PFELVKMEFD EKELRREISY AIKNIHGIRT
410 420 430 440 450
GLFTPDLAFE ATVKKQVQKL KEPSIKCVDM VVSELTSTIR KCSEKLQQYP
460 470 480 490 500
RLREEMERIV TTHIREREGR TKEQVMLLID IELAYMNTNH EDFIGFANAQ
510 520 530 540 550
QRSNQMNKKK TSGNQDEILV IRKGWLTINN IGIMKGGSKE YWFVLTAENL
560 570 580 590 600
SWYKDDEEKE KKYMLSVDNL KLRDVEKGFM SSKHIFALFN TEQRNVYKDY
610 620 630 640 650
RQLELACETQ EEVDSWKASF LRAGVYPERV GDKEKASETE ENGSDSFMHS
660 670 680 690 700
MDPQLERQVE TIRNLVDSYM AIVNKTVRDL MPKTIMHLMI NNTKEFIFSE
710 720 730 740 750
LLANLYSCGD QNTLMEESAE QAQRRDEMLR MYHALKEALS IIGDINTTTV
760 770 780 790 800
STPMPPPVDD SWLQVQSVPA GRRSPTSSPT PQRRAPAVPP ARPGSRGPAP
810 820 830 840 850
GPPPAGSALG GAPPVPSRPG ASPDPFGPPP QVPSRPNRAP PGVPSRSGQA
860
SPSRPESPRP PFDL
Length:864
Mass (Da):97,295
Last modified:June 10, 2008 - v2
Checksum:i02EC9B533CC2EC83
GO
Isoform 2 (identifier: P21575-2) [UniParc]FASTAAdd to basket
Also known as: ab

The sequence of this isoform differs from the canonical sequence as follows:
     845-864: SRSGQASPSRPESPRPPFDL → RITISDP

Note: Localized to numerous punctate spots of various sizes distributed along the plasma membrane and throughout the cytoplasm.
Show »
Length:851
Mass (Da):95,927
Checksum:i5D9294C5D1015A88
GO
Isoform 3 (identifier: P21575-3) [UniParc]FASTAAdd to basket
Also known as: ac

The sequence of this isoform differs from the canonical sequence as follows:
     766-814: QSVPAGRRSP...AGSALGGAPP → AHVQPHAAAP...PRALQAGGFP
     815-864: Missing.

Show »
Length:814
Mass (Da):92,376
Checksum:iA36766D15D5AC466
GO
Isoform 4 (identifier: P21575-4) [UniParc]FASTAAdd to basket
Also known as: ad

The sequence of this isoform differs from the canonical sequence as follows:
     845-864: SRSGQASPSRPESPRPPFDL → SQPIGSGKSVPS

Show »
Length:856
Mass (Da):96,270
Checksum:i727CC965C3929D99
GO
Isoform 5 (identifier: P21575-5) [UniParc]FASTAAdd to basket
Also known as: ba

The sequence of this isoform differs from the canonical sequence as follows:
     407-444: LAFEATVKKQ...LTSTIRKCSE → MAFETIVKKQ...LISTVRQCTK

Show »
Length:864
Mass (Da):97,424
Checksum:i4E27474E413CCEE7
GO
Isoform 6 (identifier: P21575-6) [UniParc]FASTAAdd to basket
Also known as: bb

The sequence of this isoform differs from the canonical sequence as follows:
     407-444: LAFEATVKKQ...LTSTIRKCSE → MAFETIVKKQ...LISTVRQCTK
     845-864: SRSGQASPSRPESPRPPFDL → RITISDP

Note: Diffuse cytoplasmic distribution with some localization to the Golgi apparatus.
Show »
Length:851
Mass (Da):96,057
Checksum:i5FE0938290E39F51
GO
Isoform 7 (identifier: P21575-7) [UniParc]FASTAAdd to basket
Also known as: bc

The sequence of this isoform differs from the canonical sequence as follows:
     407-444: LAFEATVKKQ...LTSTIRKCSE → MAFETIVKKQ...LISTVRQCTK
     766-814: QSVPAGRRSP...AGSALGGAPP → AHVQPHAAAP...PRALQAGGFP
     815-864: Missing.

Show »
Length:814
Mass (Da):92,505
Checksum:i686364B0BFE8865C
GO
Isoform 8 (identifier: P21575-8) [UniParc]FASTAAdd to basket
Also known as: bd

The sequence of this isoform differs from the canonical sequence as follows:
     407-444: LAFEATVKKQ...LTSTIRKCSE → MAFETIVKKQ...LISTVRQCTK
     845-864: SRSGQASPSRPESPRPPFDL → SQPIGSGKSVPS

Show »
Length:856
Mass (Da):96,399
Checksum:i1A01F8B610A0EF9E
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_034033407 – 444LAFEA…RKCSE → MAFETIVKKQVKKIREPCLK CVDMVISELISTVRQCTK in isoform 5, isoform 6, isoform 7 and isoform 8. CuratedAdd BLAST38
Alternative sequenceiVSP_034034766 – 814QSVPA…GGAPP → AHVQPHAAAPSPRRAPSPAR VAGPCSWASACWIRPGGGSP RALQAGGFP in isoform 3 and isoform 7. CuratedAdd BLAST49
Alternative sequenceiVSP_034035815 – 864Missing in isoform 3 and isoform 7. CuratedAdd BLAST50
Alternative sequenceiVSP_034037845 – 864SRSGQ…PPFDL → RITISDP in isoform 2 and isoform 6. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_034036845 – 864SRSGQ…PPFDL → SQPIGSGKSVPS in isoform 4 and isoform 8. CuratedAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54531 mRNA Translation: CAA38397.1
CO398357 mRNA No translation available.
CB583951 mRNA No translation available.
CB708564 mRNA No translation available.
PIRiS11508
RefSeqiNP_542420.1, NM_080689.4 [P21575-2]
UniGeneiRn.10354

Genome annotation databases

EnsembliENSRNOT00000047921; ENSRNOP00000041582; ENSRNOG00000033835 [P21575-1]
ENSRNOT00000064039; ENSRNOP00000060845; ENSRNOG00000033835 [P21575-2]
GeneIDi140694
KEGGirno:140694
UCSCiRGD:71096 rat [P21575-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDYN1_RAT
AccessioniPrimary (citable) accession number: P21575
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: June 10, 2008
Last modified: May 23, 2018
This is version 177 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health