Reviewed, UniProtKB/Swiss-Prot P21575 (DYN1_RAT)

Names and origin

Protein names

Recommended name:
    Dynamin-1
    EC=3.6.5.5
Alternative name(s):
    D100
    Dynamin, brain
    B-dynamin

Gene names

Name:	Dnm1
Synonyms:	Dnm

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	864 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.
Catalytic activity	GTP + H2O = GDP + phosphate.
Subunit structure	Interacts with CAV1 and SH3GLB1 By similarity. Binds SH3GL1, SH3GL2 and SH3GL3.
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton. Note: Microtubule-associated. Isoform-specific localization. Ref.7 Isoform 2: Cytoplasm. Ref.7 Isoform 6: Cytoplasm. Golgi apparatus. Ref.7
Tissue specificity	Brain-specific (peripheral sensory neurons). Ref.7
Developmental stage	Expressed in neurons after maturation.
Post-translational modification	Constitutively phosphorylated by CDK5 in nerve terminals and dephosphorylated by calcineurin when synaptic vesicle endocytosis (SVE) is stimulated by depolarization-dependent calcium influx. Ref.3 Ref.8 Ref.9
Sequence similarities	Belongs to the dynamin family. Contains 1 GED domain. Contains 1 PH domain.
Caution	Absence of tyrosine and threonine phosphorylation is demonstrated in Ref.3. However, tyrosine and threonine phosphorylation may still occur in different experimental conditions.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cytoplasm Cytoskeleton Golgi apparatus Microtubule
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase Motor protein
PTM	Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	receptor internalization Inferred from mutant phenotype. Source: UniProtKB
Cellular component	cytosol Inferred from Experiment. Source: Reactome microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from Experiment. Source: Reactome identical protein binding Traceable author statement. Source: RGD motor activity Inferred from electronic annotation. Source: UniProtKB-KW protein complex binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Amph	O08838	1	EBI-80070,EBI-80080
Bin1	O08839	1	EBI-80070,EBI-80095
NCK1	P16333	1	EBI-80070,EBI-389883	From a different organism.
Sh3gl1	O35964	1	EBI-80070,EBI-1149235
Sh3gl2	O35179	1	EBI-80070,EBI-1149197
Sh3gl3	O35180	1	EBI-80070,EBI-1149266

Alternative products

This entry describes 8 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P21575-1)

Also known as: aa;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P21575-2)

Also known as: ab;

The sequence of this isoform differs from the canonical sequence as follows:
     845-864: SRSGQASPSRPESPRPPFDL → RITISDP

Note: Localized to numerous punctate spots of various sizes distributed along the plasma membrane and throughout the cytoplasm.

Isoform 3 (identifier: P21575-3)

Also known as: ac;

The sequence of this isoform differs from the canonical sequence as follows:
     766-814: QSVPAGRRSP...AGSALGGAPP → AHVQPHAAAP...PRALQAGGFP
     815-864: Missing.

Isoform 4 (identifier: P21575-4)

Also known as: ad;

The sequence of this isoform differs from the canonical sequence as follows:
     845-864: SRSGQASPSRPESPRPPFDL → SQPIGSGKSVPS

Isoform 5 (identifier: P21575-5)

Also known as: ba;

The sequence of this isoform differs from the canonical sequence as follows:
     407-444: LAFEATVKKQ...LTSTIRKCSE → MAFETIVKKQ...LISTVRQCTK

Isoform 6 (identifier: P21575-6)

Also known as: bb;

The sequence of this isoform differs from the canonical sequence as follows:
     407-444: LAFEATVKKQ...LTSTIRKCSE → MAFETIVKKQ...LISTVRQCTK
     845-864: SRSGQASPSRPESPRPPFDL → RITISDP

Note: Diffuse cytoplasmic distribution with some localization to the Golgi apparatus.

Isoform 7 (identifier: P21575-7)

Also known as: bc;

The sequence of this isoform differs from the canonical sequence as follows:
     407-444: LAFEATVKKQ...LTSTIRKCSE → MAFETIVKKQ...LISTVRQCTK
     766-814: QSVPAGRRSP...AGSALGGAPP → AHVQPHAAAP...PRALQAGGFP
     815-864: Missing.

Isoform 8 (identifier: P21575-8)

Also known as: bd;

The sequence of this isoform differs from the canonical sequence as follows:
     407-444: LAFEATVKKQ...LTSTIRKCSE → MAFETIVKKQ...LISTVRQCTK
     845-864: SRSGQASPSRPESPRPPFDL → SQPIGSGKSVPS

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 864

864

Dynamin-1

PRO_0000206565

Regions

Domain

519 – 625

107

PH

Domain

659 – 750

92

GED

Nucleotide binding

38 – 45

8

GTP Potential

Nucleotide binding

136 – 140

5

GTP Potential

Nucleotide binding

205 – 208

4

GTP Potential

Compositional bias

753 – 851

99

Pro-rich

Amino acid modifications

Modified residue

80

1

Phosphotyrosine By similarity

Modified residue

125

1

Phosphotyrosine By similarity

Modified residue

347

1

Phosphoserine Ref.3

Modified residue

354

1

Phosphotyrosine By similarity

Modified residue

512

1

Phosphoserine Ref.3

Modified residue

774

1

Phosphoserine By similarity

Modified residue

776

1

Phosphothreonine By similarity

Modified residue

778

1

Phosphoserine By similarity

Modified residue

822

1

Phosphoserine Ref.3

Modified residue

851

1

Phosphoserine Ref.3

Modified residue

857

1

Phosphoserine Ref.3

Natural variations

Alternative sequence

407 – 444

38

LAFEA…RKCSE → MAFETIVKKQVKKIREPCLK CVDMVISELISTVRQCTK in isoform 5, isoform 6, isoform 7 and isoform 8.

VSP_034033

Alternative sequence

766 – 814

49

QSVPA…GGAPP → AHVQPHAAAPSPRRAPSPAR VAGPCSWASACWIRPGGGSP RALQAGGFP in isoform 3 and isoform 7.

VSP_034034

Alternative sequence

815 – 864

50

Missing in isoform 3 and isoform 7.

VSP_034035

Alternative sequence

845 – 864

20

SRSGQ…PPFDL → RITISDP in isoform 2 and isoform 6.

VSP_034037

Alternative sequence

845 – 864

20

SRSGQ…PPFDL → SQPIGSGKSVPS in isoform 4 and isoform 8.

VSP_034036

Secondary structure

1

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864

Helix Strand Turn

Details...

Helix

9 – 20

12

Beta strand

33 – 39

7

Helix

44 – 52

9

Beta strand

69 – 75

7

Beta strand

80 – 83

4

Helix

94 – 108

15

Beta strand

120 – 126

7

Beta strand

131 – 136

6

Beta strand

146 – 148

3

Helix

152 – 164

13

Beta strand

169 – 179

11

Helix

181 – 183

3

Helix

185 – 193

9

Beta strand

198 – 205

8

Helix

207 – 209

3

Helix

217 – 220

4

Beta strand

231 – 233

3

Helix

248 – 261

14

Turn

263 – 265

3

Helix

266 – 271

6

Helix

274 – 303

30

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (aa) [UniParc]. Last modified June 10, 2008. Version 2. Checksum: 02EC9B533CC2EC83	FASTA	864	97,295
	10 20 30 40 50 60 MGNRGMEDLI PLVNRLQDAF SAIGQNADLD LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 70 80 90 100 110 120 SGIVTRRPLV LQLVNSTTEY AEFLHCKGKK FTDFEEVRLE IEAETDRVTG TNKGISPVPI 130 140 150 160 170 180 NLRVYSPHVL NLTLVDLPGM TKVPVGDQPP DIEFQIRDML MQFVTKENCL ILAVSPANSD 190 200 210 220 230 240 LANSDALKIA KEVDPQGQRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK 250 260 270 280 290 300 DIDGKKDITA ALAAERKFFL SHPSYRHLAD RMGTPYLQKV LNQQLTNHIR DTLPGLRNKL 310 320 330 340 350 360 QSQLLSIEKE VDEYKNFRPD DPARKTKALL QMVQQFAVDF EKRIEGSGDQ IDTYELSGGA 370 380 390 400 410 420 RINRIFHERF PFELVKMEFD EKELRREISY AIKNIHGIRT GLFTPDLAFE ATVKKQVQKL 430 440 450 460 470 480 KEPSIKCVDM VVSELTSTIR KCSEKLQQYP RLREEMERIV TTHIREREGR TKEQVMLLID 490 500 510 520 530 540 IELAYMNTNH EDFIGFANAQ QRSNQMNKKK TSGNQDEILV IRKGWLTINN IGIMKGGSKE 550 560 570 580 590 600 YWFVLTAENL SWYKDDEEKE KKYMLSVDNL KLRDVEKGFM SSKHIFALFN TEQRNVYKDY 610 620 630 640 650 660 RQLELACETQ EEVDSWKASF LRAGVYPERV GDKEKASETE ENGSDSFMHS MDPQLERQVE 670 680 690 700 710 720 TIRNLVDSYM AIVNKTVRDL MPKTIMHLMI NNTKEFIFSE LLANLYSCGD QNTLMEESAE 730 740 750 760 770 780 QAQRRDEMLR MYHALKEALS IIGDINTTTV STPMPPPVDD SWLQVQSVPA GRRSPTSSPT 790 800 810 820 830 840 PQRRAPAVPP ARPGSRGPAP GPPPAGSALG GAPPVPSRPG ASPDPFGPPP QVPSRPNRAP 850 860 PGVPSRSGQA SPSRPESPRP PFDL
	Isoform 2 (ab). Checksum: 5D9294C5D1015A88	FASTA	851	95,927
	10 20 30 40 50 60 MGNRGMEDLI PLVNRLQDAF SAIGQNADLD LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 70 80 90 100 110 120 SGIVTRRPLV LQLVNSTTEY AEFLHCKGKK FTDFEEVRLE IEAETDRVTG TNKGISPVPI 130 140 150 160 170 180 NLRVYSPHVL NLTLVDLPGM TKVPVGDQPP DIEFQIRDML MQFVTKENCL ILAVSPANSD 190 200 210 220 230 240 LANSDALKIA KEVDPQGQRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK 250 260 270 280 290 300 DIDGKKDITA ALAAERKFFL SHPSYRHLAD RMGTPYLQKV LNQQLTNHIR DTLPGLRNKL 310 320 330 340 350 360 QSQLLSIEKE VDEYKNFRPD DPARKTKALL QMVQQFAVDF EKRIEGSGDQ IDTYELSGGA 370 380 390 400 410 420 RINRIFHERF PFELVKMEFD EKELRREISY AIKNIHGIRT GLFTPDLAFE ATVKKQVQKL 430 440 450 460 470 480 KEPSIKCVDM VVSELTSTIR KCSEKLQQYP RLREEMERIV TTHIREREGR TKEQVMLLID 490 500 510 520 530 540 IELAYMNTNH EDFIGFANAQ QRSNQMNKKK TSGNQDEILV IRKGWLTINN IGIMKGGSKE 550 560 570 580 590 600 YWFVLTAENL SWYKDDEEKE KKYMLSVDNL KLRDVEKGFM SSKHIFALFN TEQRNVYKDY 610 620 630 640 650 660 RQLELACETQ EEVDSWKASF LRAGVYPERV GDKEKASETE ENGSDSFMHS MDPQLERQVE 670 680 690 700 710 720 TIRNLVDSYM AIVNKTVRDL MPKTIMHLMI NNTKEFIFSE LLANLYSCGD QNTLMEESAE 730 740 750 760 770 780 QAQRRDEMLR MYHALKEALS IIGDINTTTV STPMPPPVDD SWLQVQSVPA GRRSPTSSPT 790 800 810 820 830 840 PQRRAPAVPP ARPGSRGPAP GPPPAGSALG GAPPVPSRPG ASPDPFGPPP QVPSRPNRAP 850 PGVPRITISD P
	Isoform 3 (ac). Checksum: A36766D15D5AC466	FASTA	814	92,376
	10 20 30 40 50 60 MGNRGMEDLI PLVNRLQDAF SAIGQNADLD LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 70 80 90 100 110 120 SGIVTRRPLV LQLVNSTTEY AEFLHCKGKK FTDFEEVRLE IEAETDRVTG TNKGISPVPI 130 140 150 160 170 180 NLRVYSPHVL NLTLVDLPGM TKVPVGDQPP DIEFQIRDML MQFVTKENCL ILAVSPANSD 190 200 210 220 230 240 LANSDALKIA KEVDPQGQRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK 250 260 270 280 290 300 DIDGKKDITA ALAAERKFFL SHPSYRHLAD RMGTPYLQKV LNQQLTNHIR DTLPGLRNKL 310 320 330 340 350 360 QSQLLSIEKE VDEYKNFRPD DPARKTKALL QMVQQFAVDF EKRIEGSGDQ IDTYELSGGA 370 380 390 400 410 420 RINRIFHERF PFELVKMEFD EKELRREISY AIKNIHGIRT GLFTPDLAFE ATVKKQVQKL 430 440 450 460 470 480 KEPSIKCVDM VVSELTSTIR KCSEKLQQYP RLREEMERIV TTHIREREGR TKEQVMLLID 490 500 510 520 530 540 IELAYMNTNH EDFIGFANAQ QRSNQMNKKK TSGNQDEILV IRKGWLTINN IGIMKGGSKE 550 560 570 580 590 600 YWFVLTAENL SWYKDDEEKE KKYMLSVDNL KLRDVEKGFM SSKHIFALFN TEQRNVYKDY 610 620 630 640 650 660 RQLELACETQ EEVDSWKASF LRAGVYPERV GDKEKASETE ENGSDSFMHS MDPQLERQVE 670 680 690 700 710 720 TIRNLVDSYM AIVNKTVRDL MPKTIMHLMI NNTKEFIFSE LLANLYSCGD QNTLMEESAE 730 740 750 760 770 780 QAQRRDEMLR MYHALKEALS IIGDINTTTV STPMPPPVDD SWLQVAHVQP HAAAPSPRRA 790 800 810 PSPARVAGPC SWASACWIRP GGGSPRALQA GGFP
	Isoform 4 (ad). Checksum: 727CC965C3929D99	FASTA	856	96,270
	10 20 30 40 50 60 MGNRGMEDLI PLVNRLQDAF SAIGQNADLD LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 70 80 90 100 110 120 SGIVTRRPLV LQLVNSTTEY AEFLHCKGKK FTDFEEVRLE IEAETDRVTG TNKGISPVPI 130 140 150 160 170 180 NLRVYSPHVL NLTLVDLPGM TKVPVGDQPP DIEFQIRDML MQFVTKENCL ILAVSPANSD 190 200 210 220 230 240 LANSDALKIA KEVDPQGQRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK 250 260 270 280 290 300 DIDGKKDITA ALAAERKFFL SHPSYRHLAD RMGTPYLQKV LNQQLTNHIR DTLPGLRNKL 310 320 330 340 350 360 QSQLLSIEKE VDEYKNFRPD DPARKTKALL QMVQQFAVDF EKRIEGSGDQ IDTYELSGGA 370 380 390 400 410 420 RINRIFHERF PFELVKMEFD EKELRREISY AIKNIHGIRT GLFTPDLAFE ATVKKQVQKL 430 440 450 460 470 480 KEPSIKCVDM VVSELTSTIR KCSEKLQQYP RLREEMERIV TTHIREREGR TKEQVMLLID 490 500 510 520 530 540 IELAYMNTNH EDFIGFANAQ QRSNQMNKKK TSGNQDEILV IRKGWLTINN IGIMKGGSKE 550 560 570 580 590 600 YWFVLTAENL SWYKDDEEKE KKYMLSVDNL KLRDVEKGFM SSKHIFALFN TEQRNVYKDY 610 620 630 640 650 660 RQLELACETQ EEVDSWKASF LRAGVYPERV GDKEKASETE ENGSDSFMHS MDPQLERQVE 670 680 690 700 710 720 TIRNLVDSYM AIVNKTVRDL MPKTIMHLMI NNTKEFIFSE LLANLYSCGD QNTLMEESAE 730 740 750 760 770 780 QAQRRDEMLR MYHALKEALS IIGDINTTTV STPMPPPVDD SWLQVQSVPA GRRSPTSSPT 790 800 810 820 830 840 PQRRAPAVPP ARPGSRGPAP GPPPAGSALG GAPPVPSRPG ASPDPFGPPP QVPSRPNRAP 850 PGVPSQPIGS GKSVPS
	Isoform 5 (ba). Checksum: 4E27474E413CCEE7	FASTA	864	97,424
	10 20 30 40 50 60 MGNRGMEDLI PLVNRLQDAF SAIGQNADLD LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 70 80 90 100 110 120 SGIVTRRPLV LQLVNSTTEY AEFLHCKGKK FTDFEEVRLE IEAETDRVTG TNKGISPVPI 130 140 150 160 170 180 NLRVYSPHVL NLTLVDLPGM TKVPVGDQPP DIEFQIRDML MQFVTKENCL ILAVSPANSD 190 200 210 220 230 240 LANSDALKIA KEVDPQGQRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK 250 260 270 280 290 300 DIDGKKDITA ALAAERKFFL SHPSYRHLAD RMGTPYLQKV LNQQLTNHIR DTLPGLRNKL 310 320 330 340 350 360 QSQLLSIEKE VDEYKNFRPD DPARKTKALL QMVQQFAVDF EKRIEGSGDQ IDTYELSGGA 370 380 390 400 410 420 RINRIFHERF PFELVKMEFD EKELRREISY AIKNIHGIRT GLFTPDMAFE TIVKKQVKKI 430 440 450 460 470 480 REPCLKCVDM VISELISTVR QCTKKLQQYP RLREEMERIV TTHIREREGR TKEQVMLLID 490 500 510 520 530 540 IELAYMNTNH EDFIGFANAQ QRSNQMNKKK TSGNQDEILV IRKGWLTINN IGIMKGGSKE 550 560 570 580 590 600 YWFVLTAENL SWYKDDEEKE KKYMLSVDNL KLRDVEKGFM SSKHIFALFN TEQRNVYKDY 610 620 630 640 650 660 RQLELACETQ EEVDSWKASF LRAGVYPERV GDKEKASETE ENGSDSFMHS MDPQLERQVE 670 680 690 700 710 720 TIRNLVDSYM AIVNKTVRDL MPKTIMHLMI NNTKEFIFSE LLANLYSCGD QNTLMEESAE 730 740 750 760 770 780 QAQRRDEMLR MYHALKEALS IIGDINTTTV STPMPPPVDD SWLQVQSVPA GRRSPTSSPT 790 800 810 820 830 840 PQRRAPAVPP ARPGSRGPAP GPPPAGSALG GAPPVPSRPG ASPDPFGPPP QVPSRPNRAP 850 860 PGVPSRSGQA SPSRPESPRP PFDL
	Isoform 6 (bb). Checksum: 5FE0938290E39F51	FASTA	851	96,057
	10 20 30 40 50 60 MGNRGMEDLI PLVNRLQDAF SAIGQNADLD LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 70 80 90 100 110 120 SGIVTRRPLV LQLVNSTTEY AEFLHCKGKK FTDFEEVRLE IEAETDRVTG TNKGISPVPI 130 140 150 160 170 180 NLRVYSPHVL NLTLVDLPGM TKVPVGDQPP DIEFQIRDML MQFVTKENCL ILAVSPANSD 190 200 210 220 230 240 LANSDALKIA KEVDPQGQRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK 250 260 270 280 290 300 DIDGKKDITA ALAAERKFFL SHPSYRHLAD RMGTPYLQKV LNQQLTNHIR DTLPGLRNKL 310 320 330 340 350 360 QSQLLSIEKE VDEYKNFRPD DPARKTKALL QMVQQFAVDF EKRIEGSGDQ IDTYELSGGA 370 380 390 400 410 420 RINRIFHERF PFELVKMEFD EKELRREISY AIKNIHGIRT GLFTPDMAFE TIVKKQVKKI 430 440 450 460 470 480 REPCLKCVDM VISELISTVR QCTKKLQQYP RLREEMERIV TTHIREREGR TKEQVMLLID 490 500 510 520 530 540 IELAYMNTNH EDFIGFANAQ QRSNQMNKKK TSGNQDEILV IRKGWLTINN IGIMKGGSKE 550 560 570 580 590 600 YWFVLTAENL SWYKDDEEKE KKYMLSVDNL KLRDVEKGFM SSKHIFALFN TEQRNVYKDY 610 620 630 640 650 660 RQLELACETQ EEVDSWKASF LRAGVYPERV GDKEKASETE ENGSDSFMHS MDPQLERQVE 670 680 690 700 710 720 TIRNLVDSYM AIVNKTVRDL MPKTIMHLMI NNTKEFIFSE LLANLYSCGD QNTLMEESAE 730 740 750 760 770 780 QAQRRDEMLR MYHALKEALS IIGDINTTTV STPMPPPVDD SWLQVQSVPA GRRSPTSSPT 790 800 810 820 830 840 PQRRAPAVPP ARPGSRGPAP GPPPAGSALG GAPPVPSRPG ASPDPFGPPP QVPSRPNRAP 850 PGVPRITISD P
	Isoform 7 (bc). Checksum: 686364B0BFE8865C	FASTA	814	92,505
	10 20 30 40 50 60 MGNRGMEDLI PLVNRLQDAF SAIGQNADLD LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 70 80 90 100 110 120 SGIVTRRPLV LQLVNSTTEY AEFLHCKGKK FTDFEEVRLE IEAETDRVTG TNKGISPVPI 130 140 150 160 170 180 NLRVYSPHVL NLTLVDLPGM TKVPVGDQPP DIEFQIRDML MQFVTKENCL ILAVSPANSD 190 200 210 220 230 240 LANSDALKIA KEVDPQGQRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK 250 260 270 280 290 300 DIDGKKDITA ALAAERKFFL SHPSYRHLAD RMGTPYLQKV LNQQLTNHIR DTLPGLRNKL 310 320 330 340 350 360 QSQLLSIEKE VDEYKNFRPD DPARKTKALL QMVQQFAVDF EKRIEGSGDQ IDTYELSGGA 370 380 390 400 410 420 RINRIFHERF PFELVKMEFD EKELRREISY AIKNIHGIRT GLFTPDMAFE TIVKKQVKKI 430 440 450 460 470 480 REPCLKCVDM VISELISTVR QCTKKLQQYP RLREEMERIV TTHIREREGR TKEQVMLLID 490 500 510 520 530 540 IELAYMNTNH EDFIGFANAQ QRSNQMNKKK TSGNQDEILV IRKGWLTINN IGIMKGGSKE 550 560 570 580 590 600 YWFVLTAENL SWYKDDEEKE KKYMLSVDNL KLRDVEKGFM SSKHIFALFN TEQRNVYKDY 610 620 630 640 650 660 RQLELACETQ EEVDSWKASF LRAGVYPERV GDKEKASETE ENGSDSFMHS MDPQLERQVE 670 680 690 700 710 720 TIRNLVDSYM AIVNKTVRDL MPKTIMHLMI NNTKEFIFSE LLANLYSCGD QNTLMEESAE 730 740 750 760 770 780 QAQRRDEMLR MYHALKEALS IIGDINTTTV STPMPPPVDD SWLQVAHVQP HAAAPSPRRA 790 800 810 PSPARVAGPC SWASACWIRP GGGSPRALQA GGFP
	Isoform 8 (bd). Checksum: 1A01F8B610A0EF9E	FASTA	856	96,399
	10 20 30 40 50 60 MGNRGMEDLI PLVNRLQDAF SAIGQNADLD LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG 70 80 90 100 110 120 SGIVTRRPLV LQLVNSTTEY AEFLHCKGKK FTDFEEVRLE IEAETDRVTG TNKGISPVPI 130 140 150 160 170 180 NLRVYSPHVL NLTLVDLPGM TKVPVGDQPP DIEFQIRDML MQFVTKENCL ILAVSPANSD 190 200 210 220 230 240 LANSDALKIA KEVDPQGQRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK 250 260 270 280 290 300 DIDGKKDITA ALAAERKFFL SHPSYRHLAD RMGTPYLQKV LNQQLTNHIR DTLPGLRNKL 310 320 330 340 350 360 QSQLLSIEKE VDEYKNFRPD DPARKTKALL QMVQQFAVDF EKRIEGSGDQ IDTYELSGGA 370 380 390 400 410 420 RINRIFHERF PFELVKMEFD EKELRREISY AIKNIHGIRT GLFTPDMAFE TIVKKQVKKI 430 440 450 460 470 480 REPCLKCVDM VISELISTVR QCTKKLQQYP RLREEMERIV TTHIREREGR TKEQVMLLID 490 500 510 520 530 540 IELAYMNTNH EDFIGFANAQ QRSNQMNKKK TSGNQDEILV IRKGWLTINN IGIMKGGSKE 550 560 570 580 590 600 YWFVLTAENL SWYKDDEEKE KKYMLSVDNL KLRDVEKGFM SSKHIFALFN TEQRNVYKDY 610 620 630 640 650 660 RQLELACETQ EEVDSWKASF LRAGVYPERV GDKEKASETE ENGSDSFMHS MDPQLERQVE 670 680 690 700 710 720 TIRNLVDSYM AIVNKTVRDL MPKTIMHLMI NNTKEFIFSE LLANLYSCGD QNTLMEESAE 730 740 750 760 770 780 QAQRRDEMLR MYHALKEALS IIGDINTTTV STPMPPPVDD SWLQVQSVPA GRRSPTSSPT 790 800 810 820 830 840 PQRRAPAVPP ARPGSRGPAP GPPPAGSALG GAPPVPSRPG ASPDPFGPPP QVPSRPNRAP 850 PGVPSQPIGS GKSVPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the microtubule-associated mechanochemical enzyme dynamin reveals homology with a new family of GTP-binding proteins." Obar R.A., Collins C.A., Hammarback J.A., Shpetner H.S., Vallee R.B. Nature 347:256-261(1990) [PubMed: 2144893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: Sprague-Dawley. Tissue: Brain.
[2]	Lubec G., Afjehi-Sadat L., Diao W. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 5-15; 45-54; 67-87; 91-107; 116-157; 167-188; 257-266; 300-315; 328-342; 400-415; 511-522 AND 584-594 (ISOFORM 1/2/3/4), MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord.
[3]	"The in vivo phosphorylation sites of rat brain dynamin I." Graham M.E., Anggono V., Bache N., Larsen M.R., Craft G.E., Robinson P.J. J. Biol. Chem. 282:14695-14707(2007) [PubMed: 17376771] [Abstract] Cited for: PROTEIN SEQUENCE OF 343-364; 511-522 AND 797-838 (ISOFORM 1/2/4/5/6/8), PROTEIN SEQUENCE OF 847-864 (ISOFORM 1/5), PHOSPHORYLATION AT SER-347; SER-512; SER-774; SER-778; SER-822; SER-851 AND SER-857, MASS SPECTROMETRY.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 368-581 (ISOFORM 5/6/7/8). Tissue: Brain.
[5]	"Amgen rat EST program." Amgen EST program Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 668-864 (ISOFORM 1/5). Tissue: Brain and Hypothalamus.
[6]	"The SH3p4/Sh3p8/SH3p13 protein family: binding partners for synaptojanin and dynamin via a Grb2-like Src homology 3 domain." Ringstad N., Nemoto Y., De Camilli P. Proc. Natl. Acad. Sci. U.S.A. 94:8569-8574(1997) [PubMed: 9238017] [Abstract] Cited for: INTERACTION WITH SH3GL1; SH3GL2 AND SH3GL3. Tissue: Brain.
[7]	"Differential distribution of dynamin isoforms in mammalian cells." Cao H., Garcia F., McNiven M.A. Mol. Biol. Cell 9:2595-2609(1998) [PubMed: 9725914] [Abstract] Cited for: ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[8]	"The dephosphins: dephosphorylation by calcineurin triggers synaptic vesicle endocytosis." Cousin M.A., Robinson P.J. Trends Neurosci. 24:659-665(2001) [PubMed: 11672811] [Abstract] Cited for: ROLE OF PHOSPHORYLATION.
[9]	"Cdk5 is essential for synaptic vesicle endocytosis." Tan T.C., Valova V.A., Malladi C.S., Graham M.E., Berven L.A., Jupp O.J., Hansra G., McClure S.J., Sarcevic B., Boadle R.A., Larsen M.R., Cousin M.A., Robinson P.J. Nat. Cell Biol. 5:701-710(2003) [PubMed: 12855954] [Abstract] Cited for: PHOSPHORYLATION BY CDK5.
[10]	"Dynamin I phosphorylation and the control of synaptic vesicle endocytosis." Smillie K.J., Cousin M.A. Biochem. Soc. Symp. 72:87-97(2005) [PubMed: 15649133] [Abstract] Cited for: REVIEW ON FUNCTION.
[11]	"Crystal structure of the GTPase domain of rat dynamin 1." Reubold T.F., Eschenburg S., Becker A., Leonard M., Schmid S.L., Vallee R.B., Kull F.J., Manstein D.J. Proc. Natl. Acad. Sci. U.S.A. 102:13093-13098(2005) [PubMed: 16141317] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 6-304.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X54531 mRNA. Translation: CAA38397.1.
CO398357 mRNA. No translation available.
CB583951 mRNA. No translation available.
CB708564 mRNA. No translation available.

IPI

IPI00204322.
IPI00558839.
IPI00782657.
IPI00896118.
IPI00896147.
IPI00896174.
IPI00896199.
IPI00896219.

PIR

S11508.

RefSeq

NP_542420.1.

UniGene

Rn.10354

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2AKA	X-ray	1.90	B	6-304	[»]

SMR

P21575. Positions 518-630.

ModBase

Search...

Protein-protein interaction databases

IntAct

P21575. 7 interactions.

STRING

P21575.

PTM databases

PhosphoSite

P21575.

Proteomic databases

PRIDE

P21575.

Genome annotation databases

Ensembl

ENSRNOT00000042645; ENSRNOP00000046016; ENSRNOG00000033835; Rattus norvegicus. [Genome view]
ENSRNOT00000060325; ENSRNOP00000057073; ENSRNOG00000033835; Rattus norvegicus. [Genome view]

GeneID

140694.

KEGG

rno:140694.

Organism-specific databases

CTD

140694.

RGD

71096. Dnm1.

Phylogenomic databases

HOVERGEN

P21575.

Enzyme and pathway databases

BRENDA

3.6.5.5. 248.

Gene expression databases

ArrayExpress

P21575.

Genevestigator

P21575.

GermOnline

ENSRNOG00000033835. Rattus norvegicus.

Family and domain databases

InterPro

IPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR003130. GED.
IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
[Graphical view]

Gene3D

G3DSA:2.30.29.30. PH_type. 1 hit.

Pfam

PF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]

PRINTS

PR00195. DYNAMIN.

SMART

SM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]

PROSITE

PS00410. DYNAMIN. 1 hit.
PS51388. GED. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

620679.

PMAP-CutDB

P21575.

Entry information

Entry name

DYN1_RAT

Accession

Primary (citable) accession number: P21575

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	June 10, 2008
Last modified:	November 3, 2009
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)