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Protein

Angiogenin

Gene

Ang

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei37Proton acceptorBy similarity1
Active sitei137Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Endonuclease, Hydrolase, Nuclease, Protein synthesis inhibitor

Keywords - Biological processi

Angiogenesis, Differentiation, Stress response

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-210993. Tie2 Signaling.
R-MMU-418990. Adherens junctions interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Angiogenin (EC:3.1.27.-)
Alternative name(s):
Angiogenin-1
Ribonuclease 5
Short name:
RNase 5
Gene namesi
Name:Ang
Synonyms:Ang1, Rnase5, Rnase5a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:88022. Ang.

Subcellular locationi

  • Cytoplasmic vesiclesecretory vesicle lumen By similarity
  • Secreted By similarity
  • Nucleusnucleolus By similarity

  • Note: Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Add BLAST24
ChainiPRO_000003085725 – 145AngiogeninAdd BLAST121

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei25Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi50 ↔ 1041 Publication
Disulfide bondi63 ↔ 1151 Publication
Disulfide bondi81 ↔ 1301 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP21570.
PeptideAtlasiP21570.
PRIDEiP21570.

PTM databases

PhosphoSitePlusiP21570.

Expressioni

Gene expression databases

BgeeiENSMUSG00000072115.
ExpressionAtlasiP21570. baseline and differential.
GenevisibleiP21570. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with and forms a tight 1:1 complex with RNH1. Dimerization of two such complexes may occur (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000067434.

Structurei

Secondary structure

1145
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi29 – 37Combined sources9
Helixi47 – 56Combined sources10
Turni60 – 63Combined sources4
Beta strandi65 – 70Combined sources6
Helixi74 – 78Combined sources5
Helixi79 – 81Combined sources3
Turni82 – 84Combined sources3
Beta strandi85 – 88Combined sources4
Turni89 – 91Combined sources3
Beta strandi92 – 97Combined sources6
Beta strandi99 – 108Combined sources10
Beta strandi111 – 114Combined sources4
Beta strandi116 – 124Combined sources9
Beta strandi127 – 131Combined sources5
Beta strandi134 – 138Combined sources5
Helixi140 – 142Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BWKX-ray1.50A25-145[»]
2BWLX-ray1.62A25-145[»]
ProteinModelPortaliP21570.
SMRiP21570.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21570.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni64 – 68Substrate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi55 – 59Nucleolar localization signalBy similarity5

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J3ET. Eukaryota.
ENOG410Y4FD. LUCA.
GeneTreeiENSGT00840000129759.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP21570.
KOiK16631.
OMAiFIHGNKG.
OrthoDBiEOG091G0YDD.
PhylomeDBiP21570.
TreeFamiTF333393.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21570-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAISPGPLFL IFVLGLVVIP PTLAQDDSRY TKFLTQHHDA KPKGRDDRYC
60 70 80 90 100
ERMMKRRSLT SPCKDVNTFI HGNKSNIKAI CGANGSPYRE NLRMSKSPFQ
110 120 130 140
VTTCKHTGGS PRPPCQYRAS AGFRHVVIAC ENGLPVHFDE SFFSL
Length:145
Mass (Da):16,228
Last modified:May 1, 1991 - v1
Checksum:i06944260BB764938
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22516 Genomic DNA. Translation: AAA91366.1.
BC055355 mRNA. Translation: AAH55355.1.
CCDSiCCDS27034.1.
PIRiA35932.
RefSeqiNP_001155203.1. NM_001161731.2.
NP_031473.1. NM_007447.3.
UniGeneiMm.202665.

Genome annotation databases

EnsembliENSMUST00000069011; ENSMUSP00000067434; ENSMUSG00000072115.
ENSMUST00000171688; ENSMUSP00000132084; ENSMUSG00000072115.
GeneIDi11727.
KEGGimmu:11727.
UCSCiuc007tml.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22516 Genomic DNA. Translation: AAA91366.1.
BC055355 mRNA. Translation: AAH55355.1.
CCDSiCCDS27034.1.
PIRiA35932.
RefSeqiNP_001155203.1. NM_001161731.2.
NP_031473.1. NM_007447.3.
UniGeneiMm.202665.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BWKX-ray1.50A25-145[»]
2BWLX-ray1.62A25-145[»]
ProteinModelPortaliP21570.
SMRiP21570.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000067434.

PTM databases

PhosphoSitePlusiP21570.

Proteomic databases

PaxDbiP21570.
PeptideAtlasiP21570.
PRIDEiP21570.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000069011; ENSMUSP00000067434; ENSMUSG00000072115.
ENSMUST00000171688; ENSMUSP00000132084; ENSMUSG00000072115.
GeneIDi11727.
KEGGimmu:11727.
UCSCiuc007tml.2. mouse.

Organism-specific databases

CTDi283.
MGIiMGI:88022. Ang.

Phylogenomic databases

eggNOGiENOG410J3ET. Eukaryota.
ENOG410Y4FD. LUCA.
GeneTreeiENSGT00840000129759.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP21570.
KOiK16631.
OMAiFIHGNKG.
OrthoDBiEOG091G0YDD.
PhylomeDBiP21570.
TreeFamiTF333393.

Enzyme and pathway databases

ReactomeiR-MMU-210993. Tie2 Signaling.
R-MMU-418990. Adherens junctions interactions.

Miscellaneous databases

ChiTaRSiAng. mouse.
EvolutionaryTraceiP21570.
PROiP21570.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000072115.
ExpressionAtlasiP21570. baseline and differential.
GenevisibleiP21570. MM.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANGI_MOUSE
AccessioniPrimary (citable) accession number: P21570
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 2, 2016
This is version 160 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.