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P21570

- ANGI_MOUSE

UniProt

P21570 - ANGI_MOUSE

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Protein

Angiogenin

Gene
Ang, Ang1, Rnase5, Rnase5a
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo By similarity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei37 – 371Proton acceptor By similarity
Active sitei137 – 1371Proton donor By similarity

GO - Molecular functioni

  1. actin binding Source: UniProtKB
  2. copper ion binding Source: UniProtKB
  3. DNA binding Source: UniProtKB-KW
  4. endoribonuclease activity, producing 3'-phosphomonoesters Source: InterPro
  5. heparin binding Source: UniProtKB
  6. receptor binding Source: UniProtKB
  7. ribonuclease activity Source: UniProtKB
  8. RNA binding Source: MGI

GO - Biological processi

  1. actin filament polymerization Source: UniProtKB
  2. activation of phospholipase A2 activity Source: UniProtKB
  3. activation of phospholipase C activity Source: UniProtKB
  4. angiogenesis Source: UniProtKB
  5. cell differentiation Source: UniProtKB-KW
  6. central nervous system development Source: UniProtKB
  7. diacylglycerol biosynthetic process Source: UniProtKB
  8. negative regulation of smooth muscle cell proliferation Source: UniProtKB
  9. negative regulation of translation Source: UniProtKB-KW
  10. positive regulation of endothelial cell proliferation Source: UniProtKB
  11. positive regulation of protein secretion Source: UniProtKB
  12. response to hypoxia Source: UniProtKB
  13. RNA phosphodiester bond hydrolysis Source: GOC
  14. rRNA transcription Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Endonuclease, Hydrolase, Nuclease, Protein synthesis inhibitor

Keywords - Biological processi

Angiogenesis, Differentiation, Stress response

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_211860. Tie2 Signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Angiogenin (EC:3.1.27.-)
Alternative name(s):
Angiogenin-1
Ribonuclease 5
Short name:
RNase 5
Gene namesi
Name:Ang
Synonyms:Ang1, Rnase5, Rnase5a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:88022. Ang.

Subcellular locationi

Secretedextracellular spaceextracellular matrixbasement membrane By similarity. Nucleusnucleolus By similarity
Note: Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA By similarity.

GO - Cellular componenti

  1. angiogenin-PRI complex Source: UniProtKB
  2. basal lamina Source: UniProtKB
  3. extracellular region Source: Reactome
  4. extracellular space Source: UniProtKB
  5. growth cone Source: UniProtKB
  6. neuronal cell body Source: UniProtKB
  7. nucleolus Source: UniProtKB
  8. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Add
BLAST
Chaini25 – 145121AngiogeninPRO_0000030857Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei25 – 251Pyrrolidone carboxylic acid By similarity
Disulfide bondi50 ↔ 1041 Publication
Disulfide bondi63 ↔ 1151 Publication
Disulfide bondi81 ↔ 1301 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP21570.
PRIDEiP21570.

PTM databases

PhosphoSiteiP21570.

Expressioni

Gene expression databases

ArrayExpressiP21570.
BgeeiP21570.
GenevestigatoriP21570.

Interactioni

Subunit structurei

Interacts with and forms a tight 1:1 complex with RNH1. Dimerization of two such complexes may occur By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000067434.

Structurei

Secondary structure

1
145
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 379
Helixi47 – 5610
Turni60 – 634
Beta strandi65 – 706
Helixi74 – 785
Helixi79 – 813
Turni82 – 843
Beta strandi85 – 884
Turni89 – 913
Beta strandi92 – 976
Beta strandi99 – 10810
Beta strandi111 – 1144
Beta strandi116 – 1249
Beta strandi127 – 1315
Beta strandi134 – 1385
Helixi140 – 1423

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BWKX-ray1.50A25-145[»]
2BWLX-ray1.62A25-145[»]
ProteinModelPortaliP21570.
SMRiP21570. Positions 28-143.

Miscellaneous databases

EvolutionaryTraceiP21570.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni64 – 685Substrate binding By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi55 – 595Nucleolar localization signal By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG283332.
HOGENOMiHOG000276883.
HOVERGENiHBG008396.
InParanoidiP21570.
KOiK16631.
OMAiPCKYRAT.
OrthoDBiEOG7J1826.
PhylomeDBiP21570.
TreeFamiTF333393.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21570-1 [UniParc]FASTAAdd to Basket

« Hide

MAISPGPLFL IFVLGLVVIP PTLAQDDSRY TKFLTQHHDA KPKGRDDRYC    50
ERMMKRRSLT SPCKDVNTFI HGNKSNIKAI CGANGSPYRE NLRMSKSPFQ 100
VTTCKHTGGS PRPPCQYRAS AGFRHVVIAC ENGLPVHFDE SFFSL 145
Length:145
Mass (Da):16,228
Last modified:May 1, 1991 - v1
Checksum:i06944260BB764938
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U22516 Genomic DNA. Translation: AAA91366.1.
BC055355 mRNA. Translation: AAH55355.1.
CCDSiCCDS27034.1.
PIRiA35932.
RefSeqiNP_001155203.1. NM_001161731.2.
NP_031473.1. NM_007447.3.
UniGeneiMm.202665.

Genome annotation databases

EnsembliENSMUST00000069011; ENSMUSP00000067434; ENSMUSG00000072115.
ENSMUST00000171688; ENSMUSP00000132084; ENSMUSG00000072115.
GeneIDi11727.
KEGGimmu:11727.
UCSCiuc007tml.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U22516 Genomic DNA. Translation: AAA91366.1 .
BC055355 mRNA. Translation: AAH55355.1 .
CCDSi CCDS27034.1.
PIRi A35932.
RefSeqi NP_001155203.1. NM_001161731.2.
NP_031473.1. NM_007447.3.
UniGenei Mm.202665.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2BWK X-ray 1.50 A 25-145 [» ]
2BWL X-ray 1.62 A 25-145 [» ]
ProteinModelPortali P21570.
SMRi P21570. Positions 28-143.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000067434.

PTM databases

PhosphoSitei P21570.

Proteomic databases

PaxDbi P21570.
PRIDEi P21570.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000069011 ; ENSMUSP00000067434 ; ENSMUSG00000072115 .
ENSMUST00000171688 ; ENSMUSP00000132084 ; ENSMUSG00000072115 .
GeneIDi 11727.
KEGGi mmu:11727.
UCSCi uc007tml.2. mouse.

Organism-specific databases

CTDi 283.
MGIi MGI:88022. Ang.

Phylogenomic databases

eggNOGi NOG283332.
HOGENOMi HOG000276883.
HOVERGENi HBG008396.
InParanoidi P21570.
KOi K16631.
OMAi PCKYRAT.
OrthoDBi EOG7J1826.
PhylomeDBi P21570.
TreeFami TF333393.

Enzyme and pathway databases

Reactomei REACT_211860. Tie2 Signaling.

Miscellaneous databases

ChiTaRSi ANG. mouse.
EvolutionaryTracei P21570.
NextBioi 279409.
PROi P21570.
SOURCEi Search...

Gene expression databases

ArrayExpressi P21570.
Bgeei P21570.
Genevestigatori P21570.

Family and domain databases

Gene3Di 3.10.130.10. 1 hit.
InterProi IPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view ]
PANTHERi PTHR11437. PTHR11437. 1 hit.
Pfami PF00074. RnaseA. 1 hit.
[Graphical view ]
PRINTSi PR00794. RIBONUCLEASE.
ProDomi PD000535. RNaseA. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00092. RNAse_Pc. 1 hit.
[Graphical view ]
SUPFAMi SSF54076. SSF54076. 1 hit.
PROSITEi PS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and sequencing of mouse angiogenin DNA."
    Bond M.D., Vallee B.L.
    Biochem. Biophys. Res. Commun. 171:988-995(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  3. "Characterization and sequencing of rabbit, pig and mouse angiogenins: discernment of functionally important residues and regions."
    Bond M.D., Strydom D.J., Vallee B.L.
    Biochim. Biophys. Acta 1162:177-186(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
    Tissue: Serum.
  4. "Stress induces tRNA cleavage by angiogenin in mammalian cells."
    Fu H., Feng J., Liu Q., Sun F., Tie Y., Zhu J., Xing R., Sun Z., Zheng X.
    FEBS Lett. 583:437-442(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Structure of murine angiogenin: features of the substrate- and cell-binding regions and prospects for inhibitor-binding studies."
    Holloway D.E., Chavali G.B., Hares M.C., Subramanian V., Acharya K.R.
    Acta Crystallogr. D 61:1568-1578(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 25-145, DISULFIDE BONDS.

Entry informationi

Entry nameiANGI_MOUSE
AccessioniPrimary (citable) accession number: P21570
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: September 3, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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