Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Signal recognition particle 54 kDa protein homolog

Gene

srp54

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi108 – 115GTPBy similarity8
Nucleotide bindingi190 – 194GTPBy similarity5
Nucleotide bindingi248 – 251GTPBy similarity4

GO - Molecular functioni

  • 7S RNA binding Source: PomBase
  • GTPase activity Source: PomBase
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • SRP-dependent cotranslational protein targeting to membrane Source: PomBase

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle 54 kDa protein homolog
Short name:
SRP54
Gene namesi
Name:srp54
ORF Names:SPCC188.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC188.06c
PomBaseiSPCC188.06c srp54

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Signal recognition particle

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi110Q → K or R: Lethal. 1 Publication1
Mutagenesisi114K → G or R: Lethal. 1 Publication1
Mutagenesisi115T → N: Lethal. 1 Publication1
Mutagenesisi138D → N: Lethal. 1 Publication1
Mutagenesisi194R → L or H: Cold-sensitive. 1 Publication1
Mutagenesisi248T → N: Cold-sensitive. 1 Publication1
Mutagenesisi248T → R or K: Lethal. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001012011 – 522Signal recognition particle 54 kDa protein homologAdd BLAST522

Proteomic databases

MaxQBiP21565
PaxDbiP21565
PRIDEiP21565

PTM databases

iPTMnetiP21565

Interactioni

Subunit structurei

Fungal signal recognition particle consists of a 7S RNA molecule (scR1) and at least six protein subunits: srp72, srp68, srp54, sec65, srp21 and srp14.By similarity

Protein-protein interaction databases

BioGridi275858, 3 interactors
IntActiP21565, 3 interactors
MINTiP21565
STRINGi4896.SPCC188.06c.1

Structurei

3D structure databases

ProteinModelPortaliP21565
SMRiP21565
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 295G-domainAdd BLAST295
Regioni296 – 522M-domainAdd BLAST227

Domaini

Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of srp19 and also binds the signal sequence.

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000036165
InParanoidiP21565
KOiK03106
OMAiDTAGRHK
OrthoDBiEOG092C1WCZ
PhylomeDBiP21565

Family and domain databases

Gene3Di1.10.260.30, 1 hit
HAMAPiMF_00306 SRP54, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR027417 P-loop_NTPase
IPR036891 Signal_recog_part_SRP54_M_sf
IPR013822 Signal_recog_particl_SRP54_hlx
IPR004125 Signal_recog_particle_SRP54_M
IPR022941 SRP54
IPR006325 SRP54_euk
IPR000897 SRP54_GTPase_dom
PANTHERiPTHR11564 PTHR11564, 1 hit
PfamiView protein in Pfam
PF00448 SRP54, 1 hit
PF02881 SRP54_N, 1 hit
PF02978 SRP_SPB, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00962 SRP54, 1 hit
SM00963 SRP54_N, 1 hit
SUPFAMiSSF47446 SSF47446, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01425 SRP54_euk, 1 hit
PROSITEiView protein in PROSITE
PS00300 SRP54, 1 hit

Sequencei

Sequence statusi: Complete.

P21565-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVFADLGRRL NSALGDFSKA TSVNEELVDT LLKNICTALL ETDVNVRLVQ
60 70 80 90 100
ELRSNIKKKI NVSTLPQGIN GKRIVQKAVF DELCSLVDPK VDAFTPKKGR
110 120 130 140 150
PSVIMMVGLQ GSGKTTTCSK LALHYQRRGL KSCLVAADTF RAGAFDQLKQ
160 170 180 190 200
NAIKARVPYF GSYTETDPVV IAKEGVDKFK NDRFDVIIVD TSGRHQQEQE
210 220 230 240 250
LFAEMVEISD AIRPDQTIMI LDASIGQAAE SQSKAFKETA DFGAVIITKL
260 270 280 290 300
DGHAKGGGAL SAVAATKTPI VFIGTGEHIN DLERFSPRSF ISKLLGLGDL
310 320 330 340 350
EGLMEHVQSL DFDKKNMVKN LEQGKFTVRD FRDQLGNIMK LGPLSKMASM
360 370 380 390 400
IPGMSNMMNG MNDEEGSLRM KRMLYIVDSM TEQELDSDGL LFVEQPSRVL
410 420 430 440 450
RVARGSGTSV LEVEETISQV RVFAQMAKKI GGKDGILGKL GGNPAAALKK
460 470 480 490 500
DPRQLAAMQK RMQAMGMGGG MPGLNPGSMN FGDISKMANM LMGGGGPGGA
510 520
GGMDFSGMLN QFQNMQKPPR RR
Length:522
Mass (Da):56,698
Last modified:May 1, 1991 - v1
Checksum:iEA21E48F82CC97C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51613 Genomic DNA Translation: CAA35951.1
M55518 Genomic DNA Translation: AAA35344.1
CU329672 Genomic DNA Translation: CAB41226.1
PIRiA33644
RefSeqiNP_588209.1, NM_001023199.2

Genome annotation databases

EnsemblFungiiSPCC188.06c.1; SPCC188.06c.1:pep; SPCC188.06c
GeneIDi2539290
KEGGispo:SPCC188.06c

Similar proteinsi

Entry informationi

Entry nameiSRP54_SCHPO
AccessioniPrimary (citable) accession number: P21565
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: March 28, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health