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Protein

Signal recognition particle 54 kDa protein homolog

Gene

srp54

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi108 – 1158GTPBy similarity
Nucleotide bindingi190 – 1945GTPBy similarity
Nucleotide bindingi248 – 2514GTPBy similarity

GO - Molecular functioni

  • 7S RNA binding Source: PomBase
  • GTPase activity Source: PomBase
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • SRP-dependent cotranslational protein targeting to membrane Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle 54 kDa protein homolog
Short name:
SRP54
Gene namesi
Name:srp54
ORF Names:SPCC188.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC188.06c.
PomBaseiSPCC188.06c. srp54.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • endoplasmic reticulum Source: PomBase
  • signal recognition particle, endoplasmic reticulum targeting Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Signal recognition particle

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi110 – 1101Q → K or R: Lethal. 1 Publication
Mutagenesisi114 – 1141K → G or R: Lethal. 1 Publication
Mutagenesisi115 – 1151T → N: Lethal. 1 Publication
Mutagenesisi138 – 1381D → N: Lethal. 1 Publication
Mutagenesisi194 – 1941R → L or H: Cold-sensitive. 1 Publication
Mutagenesisi248 – 2481T → N: Cold-sensitive. 1 Publication
Mutagenesisi248 – 2481T → R or K: Lethal. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 522522Signal recognition particle 54 kDa protein homologPRO_0000101201Add
BLAST

Proteomic databases

MaxQBiP21565.

Interactioni

Subunit structurei

Fungal signal recognition particle consists of a 7S RNA molecule (scR1) and at least six protein subunits: srp72, srp68, srp54, sec65, srp21 and srp14.By similarity

Protein-protein interaction databases

BioGridi275858. 3 interactions.
IntActiP21565. 3 interactions.
MINTiMINT-4687605.

Structurei

3D structure databases

ProteinModelPortaliP21565.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 295295G-domainAdd
BLAST
Regioni296 – 522227M-domainAdd
BLAST

Domaini

Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of srp19 and also binds the signal sequence.

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000036165.
InParanoidiP21565.
KOiK03106.
OMAiMLPGMGQ.
OrthoDBiEOG7NCVCS.
PhylomeDBiP21565.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR006325. SRP54_euk.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01425. SRP54_euk. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21565-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVFADLGRRL NSALGDFSKA TSVNEELVDT LLKNICTALL ETDVNVRLVQ
60 70 80 90 100
ELRSNIKKKI NVSTLPQGIN GKRIVQKAVF DELCSLVDPK VDAFTPKKGR
110 120 130 140 150
PSVIMMVGLQ GSGKTTTCSK LALHYQRRGL KSCLVAADTF RAGAFDQLKQ
160 170 180 190 200
NAIKARVPYF GSYTETDPVV IAKEGVDKFK NDRFDVIIVD TSGRHQQEQE
210 220 230 240 250
LFAEMVEISD AIRPDQTIMI LDASIGQAAE SQSKAFKETA DFGAVIITKL
260 270 280 290 300
DGHAKGGGAL SAVAATKTPI VFIGTGEHIN DLERFSPRSF ISKLLGLGDL
310 320 330 340 350
EGLMEHVQSL DFDKKNMVKN LEQGKFTVRD FRDQLGNIMK LGPLSKMASM
360 370 380 390 400
IPGMSNMMNG MNDEEGSLRM KRMLYIVDSM TEQELDSDGL LFVEQPSRVL
410 420 430 440 450
RVARGSGTSV LEVEETISQV RVFAQMAKKI GGKDGILGKL GGNPAAALKK
460 470 480 490 500
DPRQLAAMQK RMQAMGMGGG MPGLNPGSMN FGDISKMANM LMGGGGPGGA
510 520
GGMDFSGMLN QFQNMQKPPR RR
Length:522
Mass (Da):56,698
Last modified:May 1, 1991 - v1
Checksum:iEA21E48F82CC97C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51613 Genomic DNA. Translation: CAA35951.1.
M55518 Genomic DNA. Translation: AAA35344.1.
CU329672 Genomic DNA. Translation: CAB41226.1.
PIRiA33644.
RefSeqiNP_588209.1. NM_001023199.2.

Genome annotation databases

EnsemblFungiiSPCC188.06c.1; SPCC188.06c.1:pep; SPCC188.06c.
GeneIDi2539290.
KEGGispo:SPCC188.06c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51613 Genomic DNA. Translation: CAA35951.1.
M55518 Genomic DNA. Translation: AAA35344.1.
CU329672 Genomic DNA. Translation: CAB41226.1.
PIRiA33644.
RefSeqiNP_588209.1. NM_001023199.2.

3D structure databases

ProteinModelPortaliP21565.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275858. 3 interactions.
IntActiP21565. 3 interactions.
MINTiMINT-4687605.

Proteomic databases

MaxQBiP21565.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC188.06c.1; SPCC188.06c.1:pep; SPCC188.06c.
GeneIDi2539290.
KEGGispo:SPCC188.06c.

Organism-specific databases

EuPathDBiFungiDB:SPCC188.06c.
PomBaseiSPCC188.06c. srp54.

Phylogenomic databases

HOGENOMiHOG000036165.
InParanoidiP21565.
KOiK03106.
OMAiMLPGMGQ.
OrthoDBiEOG7NCVCS.
PhylomeDBiP21565.

Miscellaneous databases

NextBioi20800457.
PROiP21565.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR006325. SRP54_euk.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01425. SRP54_euk. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Saccharomyces cerevisiae and Schizosaccharomyces pombe contain a homologue to the 54-kD subunit of the signal recognition particle that in S. cerevisiae is essential for growth."
    Hann B.C., Poritz M.A., Walter P.
    J. Cell Biol. 109:3223-3230(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "The Srp54 GTPase is essential for protein export in the fission yeast Schizosaccharomyces pombe."
    Althoff S.M., Stevens S.W., Wise J.A.
    Mol. Cell. Biol. 14:7839-7854(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF GLN-110; LYS-114; THR-115; ASP-138; ARG-194 AND THR-248.

Entry informationi

Entry nameiSRP54_SCHPO
AccessioniPrimary (citable) accession number: P21565
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: December 9, 2015
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.