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Protein

Chloride channel protein

Gene
N/A
Organism
Torpedo marmorata (Marbled electric ray)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated chloride channel. This channel is thought to ensure the high conductance of the non-innervated membrane of the electrocyte necessary for efficient current generation caused by sodium influx through the acetylcholine receptor at the innervated membrane.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei123 – 1231ChlorideBy similarity
Binding sitei418 – 4181Chloride; via amide nitrogenBy similarity
Binding sitei512 – 5121ChlorideBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride channel protein
Alternative name(s):
ClC-0
OrganismiTorpedo marmorata (Marbled electric ray)
Taxonomic identifieri7788 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataChondrichthyesElasmobranchiiBatoideaTorpediniformesTorpedinidaeTorpedo

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4848CytoplasmicBy similarityAdd
BLAST
Transmembranei49 – 8638HelicalBy similarityAdd
BLAST
Transmembranei93 – 11624HelicalBy similarityAdd
BLAST
Intramembranei125 – 1328HelicalBy similarity
Transmembranei141 – 15919HelicalBy similarityAdd
BLAST
Transmembranei166 – 18419HelicalBy similarityAdd
BLAST
Intramembranei201 – 21313HelicalBy similarityAdd
BLAST
Intramembranei217 – 2259HelicalBy similarity
Transmembranei237 – 25620HelicalBy similarityAdd
BLAST
Transmembranei283 – 31129HelicalBy similarityAdd
BLAST
Transmembranei320 – 33920HelicalBy similarityAdd
BLAST
Transmembranei388 – 40821HelicalBy similarityAdd
BLAST
Transmembranei416 – 43924HelicalBy similarityAdd
BLAST
Intramembranei456 – 47015HelicalBy similarityAdd
BLAST
Intramembranei471 – 4722Note=Loop between two helicesBy similarity
Intramembranei473 – 48412HelicalBy similarityAdd
BLAST
Intramembranei485 – 4895Note=Loop between two helicesBy similarity
Transmembranei490 – 50718HelicalBy similarityAdd
BLAST
Topological domaini508 – 805298CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 805805Chloride channel proteinPRO_0000094464Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi365 – 3651N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Homodimer. Each subunit contains a channel ("Double barreled channel").1 Publication

Protein-protein interaction databases

DIPiDIP-29086N.

Structurei

Secondary structure

1
805
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi541 – 5466Combined sources
Helixi556 – 56510Combined sources
Beta strandi569 – 5757Combined sources
Turni577 – 5793Combined sources
Beta strandi581 – 5877Combined sources
Helixi588 – 60013Combined sources
Beta strandi603 – 6053Combined sources
Helixi697 – 70711Combined sources
Helixi732 – 74211Combined sources
Beta strandi745 – 7517Combined sources
Beta strandi754 – 7607Combined sources
Helixi761 – 7699Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D4ZX-ray3.10A/B525-774[»]
ProteinModelPortaliP21564.
SMRiP21564. Positions 527-770.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21564.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini543 – 60159CBS 1PROSITE-ProRule annotationAdd
BLAST
Domaini719 – 77658CBS 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi122 – 1265Selectivity filter part_1By similarity
Motifi164 – 1685Selectivity filter part_2By similarity
Motifi416 – 4205Selectivity filter part_3By similarity

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG005332.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
3.20.20.70. 2 hits.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002242. Cl_channel-0.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01111. CLCHANNEL0.
SUPFAMiSSF81340. SSF81340. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21564-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHEKNEASG YPEAQSWKSQ EAMLGARTEV SRWRAVKNCL YRHLVKVLGE
60 70 80 90 100
DWIFLLLLGA LMALVSWAMD FIGSRGLRFY KYLFALVEGN IGLQYLVWVC
110 120 130 140 150
YPLALILFSS LFCQIVSPQA VGSGIPELKT IIRGAVLHEY LTLRTFVAKT
160 170 180 190 200
VGLTVALSAG FPLGKEGPFV HIASICATLL NQLLCFISGR REEPYYLRAD
210 220 230 240 250
ILTVGCALGI SCCFGTPLAG VLFSIEVTCS HFGVRSYWRG FLGGAFSAFI
260 270 280 290 300
FRVLSVWVKD TVTLTALFKT NFRGDIPFDL QEMPAFAIIG IASGFFGALF
310 320 330 340 350
VYLNRQIIVF MRKKNFVTKI LKKQRLIYPA VVTFVLATLR FPPGVGQFFG
360 370 380 390 400
AGLMPRETIN SLFDNYTWTK TIDPRGLGNS AQWFIPHLNI FIVMALYFVM
410 420 430 440 450
HFWMAALAVT MPVPCGAFVP VFNLGAVLGR FVGELMALLF PDGLVSNGNL
460 470 480 490 500
YHILPGEYAV IGAAAMTGAV THAVSTAVIC FELTGQISHV LPMMVAVILA
510 520 530 540 550
NMVAQGLQPS LYDSIIQIKK LPYLPELSWS SANKYNIQVG DIMVRDVTSI
560 570 580 590 600
ASTSTYGDLL HVLRQTKLKF FPFVDTPDTN TLLGSIDRTE VEGLLQRRIS
610 620 630 640 650
AYRRQPAAAA EADEEGRNGE TGASFTGEAE SSFAYIDQED AEGQQREGLE
660 670 680 690 700
AVKVQTEDPR PPSPVPAEEP TQTSGIYQKK QKGTGQVASR FEEMLTLEEI
710 720 730 740 750
YRWEQREKNV VVNFETCRID QSPFQLVEGT SLQKTHTLFS LLGLDRAYVT
760 770 780 790 800
SMGKLVGVVA LAEIQAAIEG SYQKGFRLPP PLASFRDVKH ARNSGRTATS

NSSGK
Length:805
Mass (Da):88,888
Last modified:May 1, 1991 - v1
Checksum:i4CD2FA48AE06FC6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56758 mRNA. Translation: CAA40078.1.
PIRiS13410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56758 mRNA. Translation: CAA40078.1.
PIRiS13410.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D4ZX-ray3.10A/B525-774[»]
ProteinModelPortaliP21564.
SMRiP21564. Positions 527-770.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29086N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG005332.

Miscellaneous databases

EvolutionaryTraceiP21564.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
3.20.20.70. 2 hits.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002242. Cl_channel-0.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01111. CLCHANNEL0.
SUPFAMiSSF81340. SSF81340. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCICH_TORMA
AccessioniPrimary (citable) accession number: P21564
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: May 11, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels (By similarity).By similarity

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.