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Protein

Chloride channel protein

Gene
N/A
Organism
Torpedo marmorata (Marbled electric ray)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated chloride channel. This channel is thought to ensure the high conductance of the non-innervated membrane of the electrocyte necessary for efficient current generation caused by sodium influx through the acetylcholine receptor at the innervated membrane.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei123ChlorideBy similarity1
Binding sitei418Chloride; via amide nitrogenBy similarity1
Binding sitei512ChlorideBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride channel protein
Alternative name(s):
ClC-0
OrganismiTorpedo marmorata (Marbled electric ray)
Taxonomic identifieri7788 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataChondrichthyesElasmobranchiiBatoideaTorpediniformesTorpedinidaeTorpedo

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 48CytoplasmicBy similarityAdd BLAST48
Transmembranei49 – 86HelicalBy similarityAdd BLAST38
Transmembranei93 – 116HelicalBy similarityAdd BLAST24
Intramembranei125 – 132HelicalBy similarity8
Transmembranei141 – 159HelicalBy similarityAdd BLAST19
Transmembranei166 – 184HelicalBy similarityAdd BLAST19
Intramembranei201 – 213HelicalBy similarityAdd BLAST13
Intramembranei217 – 225HelicalBy similarity9
Transmembranei237 – 256HelicalBy similarityAdd BLAST20
Transmembranei283 – 311HelicalBy similarityAdd BLAST29
Transmembranei320 – 339HelicalBy similarityAdd BLAST20
Transmembranei388 – 408HelicalBy similarityAdd BLAST21
Transmembranei416 – 439HelicalBy similarityAdd BLAST24
Intramembranei456 – 470HelicalBy similarityAdd BLAST15
Intramembranei471 – 472Note=Loop between two helicesBy similarity2
Intramembranei473 – 484HelicalBy similarityAdd BLAST12
Intramembranei485 – 489Note=Loop between two helicesBy similarity5
Transmembranei490 – 507HelicalBy similarityAdd BLAST18
Topological domaini508 – 805CytoplasmicBy similarityAdd BLAST298

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944641 – 805Chloride channel proteinAdd BLAST805

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi365N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Homodimer. Each subunit contains a channel ("Double barreled channel").1 Publication

Protein-protein interaction databases

DIPiDIP-29086N.

Structurei

Secondary structure

1805
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi541 – 546Combined sources6
Helixi556 – 565Combined sources10
Beta strandi569 – 575Combined sources7
Turni577 – 579Combined sources3
Beta strandi581 – 587Combined sources7
Helixi588 – 600Combined sources13
Beta strandi603 – 605Combined sources3
Helixi697 – 707Combined sources11
Helixi732 – 742Combined sources11
Beta strandi745 – 751Combined sources7
Beta strandi754 – 760Combined sources7
Helixi761 – 769Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D4ZX-ray3.10A/B525-774[»]
ProteinModelPortaliP21564.
SMRiP21564.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21564.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini543 – 601CBS 1PROSITE-ProRule annotationAdd BLAST59
Domaini719 – 776CBS 2PROSITE-ProRule annotationAdd BLAST58

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi122 – 126Selectivity filter part_1By similarity5
Motifi164 – 168Selectivity filter part_2By similarity5
Motifi416 – 420Selectivity filter part_3By similarity5

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG005332.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
3.20.20.70. 2 hits.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002242. Cl_channel-0.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01111. CLCHANNEL0.
SUPFAMiSSF81340. SSF81340. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21564-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHEKNEASG YPEAQSWKSQ EAMLGARTEV SRWRAVKNCL YRHLVKVLGE
60 70 80 90 100
DWIFLLLLGA LMALVSWAMD FIGSRGLRFY KYLFALVEGN IGLQYLVWVC
110 120 130 140 150
YPLALILFSS LFCQIVSPQA VGSGIPELKT IIRGAVLHEY LTLRTFVAKT
160 170 180 190 200
VGLTVALSAG FPLGKEGPFV HIASICATLL NQLLCFISGR REEPYYLRAD
210 220 230 240 250
ILTVGCALGI SCCFGTPLAG VLFSIEVTCS HFGVRSYWRG FLGGAFSAFI
260 270 280 290 300
FRVLSVWVKD TVTLTALFKT NFRGDIPFDL QEMPAFAIIG IASGFFGALF
310 320 330 340 350
VYLNRQIIVF MRKKNFVTKI LKKQRLIYPA VVTFVLATLR FPPGVGQFFG
360 370 380 390 400
AGLMPRETIN SLFDNYTWTK TIDPRGLGNS AQWFIPHLNI FIVMALYFVM
410 420 430 440 450
HFWMAALAVT MPVPCGAFVP VFNLGAVLGR FVGELMALLF PDGLVSNGNL
460 470 480 490 500
YHILPGEYAV IGAAAMTGAV THAVSTAVIC FELTGQISHV LPMMVAVILA
510 520 530 540 550
NMVAQGLQPS LYDSIIQIKK LPYLPELSWS SANKYNIQVG DIMVRDVTSI
560 570 580 590 600
ASTSTYGDLL HVLRQTKLKF FPFVDTPDTN TLLGSIDRTE VEGLLQRRIS
610 620 630 640 650
AYRRQPAAAA EADEEGRNGE TGASFTGEAE SSFAYIDQED AEGQQREGLE
660 670 680 690 700
AVKVQTEDPR PPSPVPAEEP TQTSGIYQKK QKGTGQVASR FEEMLTLEEI
710 720 730 740 750
YRWEQREKNV VVNFETCRID QSPFQLVEGT SLQKTHTLFS LLGLDRAYVT
760 770 780 790 800
SMGKLVGVVA LAEIQAAIEG SYQKGFRLPP PLASFRDVKH ARNSGRTATS

NSSGK
Length:805
Mass (Da):88,888
Last modified:May 1, 1991 - v1
Checksum:i4CD2FA48AE06FC6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56758 mRNA. Translation: CAA40078.1.
PIRiS13410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56758 mRNA. Translation: CAA40078.1.
PIRiS13410.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D4ZX-ray3.10A/B525-774[»]
ProteinModelPortaliP21564.
SMRiP21564.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29086N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG005332.

Miscellaneous databases

EvolutionaryTraceiP21564.

Family and domain databases

Gene3Di1.10.3080.10. 1 hit.
3.20.20.70. 2 hits.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002242. Cl_channel-0.
[Graphical view]
PfamiPF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01111. CLCHANNEL0.
SUPFAMiSSF81340. SSF81340. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCICH_TORMA
AccessioniPrimary (citable) accession number: P21564
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 30, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels (By similarity).By similarity

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.