P21557 (GYRA_HALL2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:

gyrA

Organism

Haloferax lucentense (strain DSM 14919 / JCM 9276 / NCIMB 13854 / Aa 2.2) (Haloferax sp. (strain Aa 2.2))

Taxonomic identifier

2254 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloferax

Protein attributes

Sequence length	82 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Miscellaneous	Mutations in DNA gyrase result in novobiocin resistance in halophilic archaebacteria. HAMAP MF_01897
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›82	›82	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145273
Experimental info
	Non-terminal residue	82	1

Sequences

	Sequence		Length	Mass (Da)
	P21557 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 8DD3543CF5111286	FASTA	82	8,847
	10 20 30 40 50 60 MSSDAPDSFE PGAGIAAEVK NARIEDEMEQ SYIDYAMSVI AGRALPDVRD GLKPVHRRIL 70 80 YAMHQAGVTS NSSHRKSSSI VG

References

[1]	"Mutations in DNA gyrase result in novobiocin resistance in halophilic archaebacteria." Holmes M.L., Dyall-Smith M.L. J. Bacteriol. 173:642-648(1991) [PubMed: 1846146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M38373 Genomic DNA. Translation: AAB09606.1.
3D structure databases
ProteinModelPortal	P21557.
SMR	P21557. Positions 42-82.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit.
Pfam	PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_HALL2

Accession

Primary (citable) accession number: P21557

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	July 27, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program