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Protein

Cannabinoid receptor 1

Gene

CNR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca2+ channel current. Isoform 2 and isoform 3 have altered ligand binding.1 Publication

GO - Molecular functioni

  • cannabinoid receptor activity Source: UniProtKB
  • drug binding Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-HSA-373076. Class A/1 (Rhodopsin-like receptors).
R-HSA-418594. G alpha (i) signalling events.
SIGNORiP21554.

Protein family/group databases

TCDBi9.A.14.2.2. the g-protein-coupled receptor (gpcr) family.

Chemistry databases

SwissLipidsiSLP:000001607.

Names & Taxonomyi

Protein namesi
Recommended name:
Cannabinoid receptor 1
Short name:
CB-R
Short name:
CB1
Alternative name(s):
CANN6
Gene namesi
Name:CNR1
Synonyms:CNR
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:2159. CNR1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 116ExtracellularSequence analysisAdd BLAST116
Transmembranei117 – 142Helical; Name=1Sequence analysisAdd BLAST26
Topological domaini143 – 154CytoplasmicSequence analysisAdd BLAST12
Transmembranei155 – 175Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini176 – 187ExtracellularSequence analysisAdd BLAST12
Transmembranei188 – 212Helical; Name=3Sequence analysisAdd BLAST25
Topological domaini213 – 232CytoplasmicSequence analysisAdd BLAST20
Transmembranei233 – 255Helical; Name=4Sequence analysisAdd BLAST23
Topological domaini256 – 273ExtracellularSequence analysisAdd BLAST18
Transmembranei274 – 299Helical; Name=5Sequence analysisAdd BLAST26
Topological domaini300 – 344CytoplasmicSequence analysisAdd BLAST45
Transmembranei345 – 365Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini366 – 377ExtracellularSequence analysisAdd BLAST12
Transmembranei378 – 399Helical; Name=7Sequence analysisAdd BLAST22
Topological domaini400 – 472CytoplasmicSequence analysisAdd BLAST73

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi1268.
OpenTargetsiENSG00000118432.
PharmGKBiPA26681.

Chemistry databases

ChEMBLiCHEMBL218.
DrugBankiDB05750. AVE-1625.
DB05372. CP-945598.
DB00470. Dronabinol.
DB00486. Nabilone.
DB06155. Rimonabant.
DB05077. SLV319.
DB05201. V24343.
GuidetoPHARMACOLOGYi56.

Polymorphism and mutation databases

BioMutaiCNR1.
DMDMi115562.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000693141 – 472Cannabinoid receptor 1Add BLAST472

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi77N-linked (GlcNAc...)Sequence analysis1
Glycosylationi83N-linked (GlcNAc...)Sequence analysis1
Glycosylationi112N-linked (GlcNAc...)Sequence analysis1
Lipidationi415S-palmitoyl cysteine1 Publication1
Modified residuei425PhosphoserineBy similarity1
Modified residuei429PhosphoserineBy similarity1

Post-translational modificationi

Palmitoylation at Cys-415 is important for recruitment at both plasma membrane and lipid rafts.1 Publication

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP21554.
PeptideAtlasiP21554.
PRIDEiP21554.

PTM databases

iPTMnetiP21554.
PhosphoSitePlusiP21554.
SwissPalmiP21554.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000118432.
CleanExiHS_CNR1.
ExpressionAtlasiP21554. baseline and differential.
GenevisibleiP21554. HS.

Organism-specific databases

HPAiCAB005603.
HPA042256.

Interactioni

Subunit structurei

Interacts (via C-terminus) with CNRIP1.1 Publication

Protein-protein interaction databases

BioGridi107668. 4 interactors.
DIPiDIP-61575N.
IntActiP21554. 1 interactor.
STRINGi9606.ENSP00000358511.

Chemistry databases

BindingDBiP21554.

Structurei

Secondary structure

1472
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi105 – 107Combined sources3
Helixi113 – 143Combined sources31
Helixi145 – 148Combined sources4
Helixi151 – 153Combined sources3
Helixi154 – 178Combined sources25
Helixi186 – 219Combined sources34
Turni221 – 223Combined sources3
Helixi224 – 227Combined sources4
Helixi230 – 249Combined sources20
Helixi250 – 253Combined sources4
Helixi257 – 260Combined sources4
Beta strandi266 – 268Combined sources3
Helixi273 – 300Combined sources28
Helixi333 – 367Combined sources35
Helixi375 – 400Combined sources26
Helixi402 – 409Combined sources8
Helixi411 – 414Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LVQNMR-A338-346[»]
1LVRNMR-A338-346[»]
2B0YNMR-A400-414[»]
2KOENMR-A377-414[»]
2MZ2NMR-A400-414[»]
2MZ3NMR-A400-414[»]
2MZANMR-A400-414[»]
5TGZX-ray2.80A99-306[»]
A332-414[»]
5U09X-ray2.60A90-301[»]
A333-421[»]
ProteinModelPortaliP21554.
SMRiP21554.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21554.

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118804.
HOVERGENiHBG051045.
InParanoidiP21554.
KOiK04277.
OMAiVAFCVMW.
OrthoDBiEOG091G05O3.
PhylomeDBiP21554.
TreeFamiTF330052.

Family and domain databases

InterProiView protein in InterPro
IPR000810. Canbinoid_rcpt_1.
IPR002230. Cnbnoid_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
PANTHERiPTHR22750:SF57. PTHR22750:SF57. 1 hit.
PfamiView protein in Pfam
PF00001. 7tm_1. 1 hit.
PIRSFiPIRSF037995. Cnoid_rcpt_1. 1 hit.
PRINTSiPR00522. CANABINOID1R.
PR00362. CANNABINOIDR.
PR00237. GPCRRHODOPSN.
SMARTiView protein in SMART
SM01381. 7TM_GPCR_Srsx. 1 hit.
PROSITEiView protein in PROSITE
PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P21554-1) [UniParc]FASTAAdd to basket
Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKSILDGLAD TTFRTITTDL LYVGSNDIQY EDIKGDMASK LGYFPQKFPL
60 70 80 90 100
TSFRGSPFQE KMTAGDNPQL VPADQVNITE FYNKSLSSFK ENEENIQCGE
110 120 130 140 150
NFMDIECFMV LNPSQQLAIA VLSLTLGTFT VLENLLVLCV ILHSRSLRCR
160 170 180 190 200
PSYHFIGSLA VADLLGSVIF VYSFIDFHVF HRKDSRNVFL FKLGGVTASF
210 220 230 240 250
TASVGSLFLT AIDRYISIHR PLAYKRIVTR PKAVVAFCLM WTIAIVIAVL
260 270 280 290 300
PLLGWNCEKL QSVCSDIFPH IDETYLMFWI GVTSVLLLFI VYAYMYILWK
310 320 330 340 350
AHSHAVRMIQ RGTQKSIIIH TSEDGKVQVT RPDQARMDIR LAKTLVLILV
360 370 380 390 400
VLIICWGPLL AIMVYDVFGK MNKLIKTVFA FCSMLCLLNS TVNPIIYALR
410 420 430 440 450
SKDLRHAFRS MFPSCEGTAQ PLDNSMGDSD CLHKHANNAA SVHRAAESCI
460 470
KSTVKIAKVT MSVSTDTSAE AL
Length:472
Mass (Da):52,858
Last modified:May 1, 1991 - v1
Checksum:i1D2E49061D12ABF2
GO
Isoform 2 (identifier: P21554-2) [UniParc]FASTAAdd to basket
Also known as: CB1a, Short

The sequence of this isoform differs from the canonical sequence as follows:
     1-89: MKSILDGLAD...EFYNKSLSSF → MALQIPPSAPSPLTSCTWAQMTFSTKTS

Note: Dubious isoform. A putative downstream initiation AUG is used to produce isoform 2 (PubMed:1718258). The use of the first AUG (same as isoform 1) gives a truncated protein of 36 AA.1 Publication
Show »
Length:411
Mass (Da):45,874
Checksum:iE3C31ACAB4066BC1
GO
Isoform 3 (identifier: P21554-3) [UniParc]FASTAAdd to basket
Also known as: CB1b

The sequence of this isoform differs from the canonical sequence as follows:
     22-54: Missing.

Show »
Length:439
Mass (Da):49,060
Checksum:iC80C6B8D640412C1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94E → G in AAH95513 (PubMed:15489334).Curated1
Sequence conflicti103M → I in AAH95513 (PubMed:15489334).Curated1
Sequence conflicti149C → R in AAH95513 (PubMed:15489334).Curated1
Sequence conflicti200F → L in AAD34320 (Ref. 5) Curated1
Sequence conflicti216I → V in AAD34320 (Ref. 5) Curated1
Sequence conflicti246V → A in AAD34320 (Ref. 5) Curated1
Sequence conflicti298L → P in AAH95513 (PubMed:15489334).Curated1
Sequence conflicti332P → S in AAI00972 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0018681 – 89MKSIL…SLSSF → MALQIPPSAPSPLTSCTWAQ MTFSTKTS in isoform 2. 1 PublicationAdd BLAST89
Alternative sequenceiVSP_01652922 – 54Missing in isoform 3. 1 PublicationAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54937 mRNA. Translation: CAA38699.1.
X81120 mRNA. Translation: CAA57018.1.
X81121 mRNA. Translation: CAA57019.1.
AY766182 mRNA. Translation: AAV35030.1.
AF107262 mRNA. Translation: AAD34320.1.
U73304 Genomic DNA. Translation: AAB18200.1.
DQ067455 mRNA. Translation: AAY68486.1.
AY225225 Genomic DNA. Translation: AAO67710.1.
AL136096 Genomic DNA. Translation: CAB96726.1.
AL136096 Genomic DNA. Translation: CAI19916.1.
AK313908 mRNA. Translation: BAG36631.1.
CH471051 Genomic DNA. Translation: EAW48574.1.
CH471051 Genomic DNA. Translation: EAW48575.1.
CH471051 Genomic DNA. Translation: EAW48576.1.
BC074811 mRNA. Translation: AAH74811.1.
BC074812 mRNA. Translation: AAH74812.1.
BC095513 mRNA. Translation: AAH95513.1.
BC100968 mRNA. Translation: AAI00969.1.
BC100969 mRNA. Translation: AAI00970.1.
BC100970 mRNA. Translation: AAI00971.1.
BC100971 mRNA. Translation: AAI00972.1.
CCDSiCCDS5015.1. [P21554-1]
CCDS5016.2. [P21554-3]
PIRiS17595.
RefSeqiNP_001153698.1. NM_001160226.1. [P21554-1]
NP_001153730.1. NM_001160258.1. [P21554-1]
NP_001153731.1. NM_001160259.1. [P21554-1]
NP_057167.2. NM_016083.4. [P21554-1]
NP_149421.2. NM_033181.3. [P21554-3]
XP_006715393.1. XM_006715330.3. [P21554-1]
XP_011533726.1. XM_011535424.1. [P21554-1]
XP_011533727.1. XM_011535425.1. [P21554-1]
XP_011533728.1. XM_011535426.1. [P21554-1]
XP_011533730.1. XM_011535428.1. [P21554-1]
XP_016865727.1. XM_017010238.1. [P21554-1]
XP_016865728.1. XM_017010239.1. [P21554-1]
XP_016865729.1. XM_017010240.1. [P21554-1]
UniGeneiHs.75110.

Genome annotation databases

EnsembliENST00000369499; ENSP00000358511; ENSG00000118432. [P21554-1]
ENST00000369501; ENSP00000358513; ENSG00000118432. [P21554-1]
ENST00000428600; ENSP00000412192; ENSG00000118432. [P21554-1]
ENST00000468898; ENSP00000420188; ENSG00000118432. [P21554-3]
ENST00000549890; ENSP00000446819; ENSG00000118432. [P21554-1]
GeneIDi1268.
KEGGihsa:1268.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54937 mRNA. Translation: CAA38699.1.
X81120 mRNA. Translation: CAA57018.1.
X81121 mRNA. Translation: CAA57019.1.
AY766182 mRNA. Translation: AAV35030.1.
AF107262 mRNA. Translation: AAD34320.1.
U73304 Genomic DNA. Translation: AAB18200.1.
DQ067455 mRNA. Translation: AAY68486.1.
AY225225 Genomic DNA. Translation: AAO67710.1.
AL136096 Genomic DNA. Translation: CAB96726.1.
AL136096 Genomic DNA. Translation: CAI19916.1.
AK313908 mRNA. Translation: BAG36631.1.
CH471051 Genomic DNA. Translation: EAW48574.1.
CH471051 Genomic DNA. Translation: EAW48575.1.
CH471051 Genomic DNA. Translation: EAW48576.1.
BC074811 mRNA. Translation: AAH74811.1.
BC074812 mRNA. Translation: AAH74812.1.
BC095513 mRNA. Translation: AAH95513.1.
BC100968 mRNA. Translation: AAI00969.1.
BC100969 mRNA. Translation: AAI00970.1.
BC100970 mRNA. Translation: AAI00971.1.
BC100971 mRNA. Translation: AAI00972.1.
CCDSiCCDS5015.1. [P21554-1]
CCDS5016.2. [P21554-3]
PIRiS17595.
RefSeqiNP_001153698.1. NM_001160226.1. [P21554-1]
NP_001153730.1. NM_001160258.1. [P21554-1]
NP_001153731.1. NM_001160259.1. [P21554-1]
NP_057167.2. NM_016083.4. [P21554-1]
NP_149421.2. NM_033181.3. [P21554-3]
XP_006715393.1. XM_006715330.3. [P21554-1]
XP_011533726.1. XM_011535424.1. [P21554-1]
XP_011533727.1. XM_011535425.1. [P21554-1]
XP_011533728.1. XM_011535426.1. [P21554-1]
XP_011533730.1. XM_011535428.1. [P21554-1]
XP_016865727.1. XM_017010238.1. [P21554-1]
XP_016865728.1. XM_017010239.1. [P21554-1]
XP_016865729.1. XM_017010240.1. [P21554-1]
UniGeneiHs.75110.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LVQNMR-A338-346[»]
1LVRNMR-A338-346[»]
2B0YNMR-A400-414[»]
2KOENMR-A377-414[»]
2MZ2NMR-A400-414[»]
2MZ3NMR-A400-414[»]
2MZANMR-A400-414[»]
5TGZX-ray2.80A99-306[»]
A332-414[»]
5U09X-ray2.60A90-301[»]
A333-421[»]
ProteinModelPortaliP21554.
SMRiP21554.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107668. 4 interactors.
DIPiDIP-61575N.
IntActiP21554. 1 interactor.
STRINGi9606.ENSP00000358511.

Chemistry databases

BindingDBiP21554.
ChEMBLiCHEMBL218.
DrugBankiDB05750. AVE-1625.
DB05372. CP-945598.
DB00470. Dronabinol.
DB00486. Nabilone.
DB06155. Rimonabant.
DB05077. SLV319.
DB05201. V24343.
GuidetoPHARMACOLOGYi56.
SwissLipidsiSLP:000001607.

Protein family/group databases

TCDBi9.A.14.2.2. the g-protein-coupled receptor (gpcr) family.
GPCRDBiSearch...

PTM databases

iPTMnetiP21554.
PhosphoSitePlusiP21554.
SwissPalmiP21554.

Polymorphism and mutation databases

BioMutaiCNR1.
DMDMi115562.

Proteomic databases

PaxDbiP21554.
PeptideAtlasiP21554.
PRIDEiP21554.

Protocols and materials databases

DNASUi1268.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369499; ENSP00000358511; ENSG00000118432. [P21554-1]
ENST00000369501; ENSP00000358513; ENSG00000118432. [P21554-1]
ENST00000428600; ENSP00000412192; ENSG00000118432. [P21554-1]
ENST00000468898; ENSP00000420188; ENSG00000118432. [P21554-3]
ENST00000549890; ENSP00000446819; ENSG00000118432. [P21554-1]
GeneIDi1268.
KEGGihsa:1268.

Organism-specific databases

CTDi1268.
DisGeNETi1268.
GeneCardsiCNR1.
H-InvDBiHIX0165039.
HGNCiHGNC:2159. CNR1.
HPAiCAB005603.
HPA042256.
MIMi114610. gene.
neXtProtiNX_P21554.
OpenTargetsiENSG00000118432.
PharmGKBiPA26681.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118804.
HOVERGENiHBG051045.
InParanoidiP21554.
KOiK04277.
OMAiVAFCVMW.
OrthoDBiEOG091G05O3.
PhylomeDBiP21554.
TreeFamiTF330052.

Enzyme and pathway databases

ReactomeiR-HSA-373076. Class A/1 (Rhodopsin-like receptors).
R-HSA-418594. G alpha (i) signalling events.
SIGNORiP21554.

Miscellaneous databases

EvolutionaryTraceiP21554.
GeneWikiiCannabinoid_receptor_type_1.
GenomeRNAii1268.
PROiPR:P21554.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000118432.
CleanExiHS_CNR1.
ExpressionAtlasiP21554. baseline and differential.
GenevisibleiP21554. HS.

Family and domain databases

InterProiView protein in InterPro
IPR000810. Canbinoid_rcpt_1.
IPR002230. Cnbnoid_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
PANTHERiPTHR22750:SF57. PTHR22750:SF57. 1 hit.
PfamiView protein in Pfam
PF00001. 7tm_1. 1 hit.
PIRSFiPIRSF037995. Cnoid_rcpt_1. 1 hit.
PRINTSiPR00522. CANABINOID1R.
PR00362. CANNABINOIDR.
PR00237. GPCRRHODOPSN.
SMARTiView protein in SMART
SM01381. 7TM_GPCR_Srsx. 1 hit.
PROSITEiView protein in PROSITE
PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCNR1_HUMAN
AccessioniPrimary (citable) accession number: P21554
Secondary accession number(s): B2R9T4
, E1P512, Q13949, Q495Z0, Q4PLI4, Q4VBM6, Q5JVL5, Q5UB37, Q9UNN0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: March 15, 2017
This is version 178 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.