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P21553

- CISY_THEAC

UniProt

P21553 - CISY_THEAC

Protein

Citrate synthase

Gene

gltA

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

    Enzyme regulationi

    Allosterically inhibited by NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei223 – 2231
    Active sitei263 – 2631
    Active sitei318 – 3181

    GO - Molecular functioni

    1. transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer Source: InterPro

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. tricarboxylic acid cycle Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Enzyme and pathway databases

    UniPathwayiUPA00223; UER00717.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Citrate synthase (EC:2.3.3.16)
    Gene namesi
    Name:gltA
    Ordered Locus Names:Ta0169
    OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
    Taxonomic identifieri273075 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
    ProteomesiUP000001024: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 385384Citrate synthasePRO_0000169978Add
    BLAST

    Proteomic databases

    PRIDEiP21553.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi273075.Ta0169.

    Structurei

    Secondary structure

    1
    385
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 113
    Beta strandi15 – 2511
    Turni26 – 294
    Beta strandi30 – 334
    Helixi38 – 436
    Helixi48 – 5710
    Helixi63 – 7412
    Helixi75 – 773
    Helixi81 – 888
    Helixi96 – 11015
    Turni118 – 1203
    Helixi121 – 14323
    Helixi157 – 16610
    Helixi172 – 18413
    Helixi192 – 20110
    Turni202 – 2043
    Helixi207 – 21812
    Turni221 – 2233
    Helixi226 – 23712
    Helixi240 – 2423
    Helixi243 – 2508
    Turni251 – 2533
    Beta strandi254 – 2563
    Helixi271 – 28515
    Helixi288 – 30821
    Helixi309 – 3113
    Turni317 – 3204
    Helixi321 – 3288
    Helixi333 – 3353
    Helixi336 – 36025
    Beta strandi367 – 3704
    Helixi381 – 3833

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2IFCX-ray1.70A/B/C/D1-385[»]
    2R26X-ray2.50A/B/C/D2-385[»]
    2R9EX-ray1.95A/B/C/D1-385[»]
    ProteinModelPortaliP21553.
    SMRiP21553. Positions 4-385.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP21553.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the citrate synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG0372.
    HOGENOMiHOG000021225.
    KOiK01647.
    OMAiSCKSRIT.

    Family and domain databases

    Gene3Di1.10.230.10. 1 hit.
    1.10.580.10. 1 hit.
    InterProiIPR011278. 2-MeCitrate/Citrate_synth_II.
    IPR016142. Citrate_synth-like_lrg_a-sub.
    IPR016143. Citrate_synth-like_sm_a-sub.
    IPR002020. Citrate_synthase-like.
    IPR016141. Citrate_synthase-like_core.
    IPR019810. Citrate_synthase_AS.
    IPR024176. Citrate_synthase_bac-typ.
    [Graphical view]
    PANTHERiPTHR11739. PTHR11739. 1 hit.
    PfamiPF00285. Citrate_synt. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001369. Citrate_synth. 1 hit.
    PRINTSiPR00143. CITRTSNTHASE.
    SUPFAMiSSF48256. SSF48256. 1 hit.
    TIGRFAMsiTIGR01800. cit_synth_II. 1 hit.
    PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P21553-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPETEEISKG LEDVNIKWTR LTTIDGNKGI LRYGGYSVED IIASGAQDEE    50
    IQYLFLYGNL PTEQELRKYK ETVQKGYKIP DFVINAIRQL PRESDAVAMQ 100
    MAAVAAMAAS ETKFKWNKDT DRDVAAEMIG RMSAITVNVY RHIMNMPAEL 150
    PKPSDSYAES FLNAAFGRKA TKEEIDAMNT ALILYTDHEV PASTTAGLVA 200
    VSTLSDMYSG ITAALAALKG PLHGGAAEAA IAQFDEIKDP AMVEKWFNDN 250
    IINGKKRLMG FGHRVYKTYD PRAKIFKGIA EKLSSKKPEV HKVYEIATKL 300
    EDFGIKAFGS KGIYPNTDYF SGIVYMSIGF PLRNNIYTAL FALSRVTGWQ 350
    AHFIEYVEEQ QRLIRPRAVY VGPAERKYVP IAERK 385
    Length:385
    Mass (Da):43,072
    Last modified:January 23, 2007 - v2
    Checksum:i9B7C2C88CEBCB690
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55282 Genomic DNA. Translation: CAA38996.1.
    AL445063 Genomic DNA. Translation: CAC11315.1.
    PIRiS13831. YKYT.
    RefSeqiNP_393647.1. NC_002578.1.
    WP_010900596.1. NC_002578.1.

    Genome annotation databases

    EnsemblBacteriaiCAC11315; CAC11315; CAC11315.
    GeneIDi1455814.
    KEGGitac:Ta0169.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55282 Genomic DNA. Translation: CAA38996.1 .
    AL445063 Genomic DNA. Translation: CAC11315.1 .
    PIRi S13831. YKYT.
    RefSeqi NP_393647.1. NC_002578.1.
    WP_010900596.1. NC_002578.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2IFC X-ray 1.70 A/B/C/D 1-385 [» ]
    2R26 X-ray 2.50 A/B/C/D 2-385 [» ]
    2R9E X-ray 1.95 A/B/C/D 1-385 [» ]
    ProteinModelPortali P21553.
    SMRi P21553. Positions 4-385.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273075.Ta0169.

    Proteomic databases

    PRIDEi P21553.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC11315 ; CAC11315 ; CAC11315 .
    GeneIDi 1455814.
    KEGGi tac:Ta0169.

    Phylogenomic databases

    eggNOGi COG0372.
    HOGENOMi HOG000021225.
    KOi K01647.
    OMAi SCKSRIT.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER00717 .

    Miscellaneous databases

    EvolutionaryTracei P21553.

    Family and domain databases

    Gene3Di 1.10.230.10. 1 hit.
    1.10.580.10. 1 hit.
    InterProi IPR011278. 2-MeCitrate/Citrate_synth_II.
    IPR016142. Citrate_synth-like_lrg_a-sub.
    IPR016143. Citrate_synth-like_sm_a-sub.
    IPR002020. Citrate_synthase-like.
    IPR016141. Citrate_synthase-like_core.
    IPR019810. Citrate_synthase_AS.
    IPR024176. Citrate_synthase_bac-typ.
    [Graphical view ]
    PANTHERi PTHR11739. PTHR11739. 1 hit.
    Pfami PF00285. Citrate_synt. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001369. Citrate_synth. 1 hit.
    PRINTSi PR00143. CITRTSNTHASE.
    SUPFAMi SSF48256. SSF48256. 1 hit.
    TIGRFAMsi TIGR01800. cit_synth_II. 1 hit.
    PROSITEi PS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Citrate synthase from the thermophilic archaebacterium Thermoplasma acidophilium. Cloning and sequencing of the gene."
      Sutherland K.J., Henneke C.M., Towner P., Hough D.W., Danson M.J.
      Eur. J. Biochem. 194:839-844(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
    2. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
      Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
      Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
    3. "Citrate synthase from the thermophilic archaebacteria Thermoplasma acidophilum and Sulfolobus acidocaldarius."
      Smith L.D., Stevenson K.J., Hough D.W., Danson M.J.
      FEBS Lett. 225:277-281(1987)
      Cited for: PROTEIN SEQUENCE OF 2-17.
    4. "The crystal structure of citrate synthase from the thermophilic archaeon, Thermoplasma acidophilum."
      Russel R.J.M., Hough D.W., Danson M.J., Taylor G.L.
      Structure 2:1157-1167(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

    Entry informationi

    Entry nameiCISY_THEAC
    AccessioniPrimary (citable) accession number: P21553
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Citrate synthase is found in nearly all cells capable of oxidative metabolism.

    Keywords - Technical termi

    3D-structure, Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3