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Protein

Citrate synthase

Gene

gltA

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Enzyme regulationi

Allosterically inhibited by NADH.

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase (gltA)
  2. no protein annotated in this organism
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2231
Active sitei2631
Active sitei3181

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

BRENDAi2.3.3.16. 6324.
UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase (EC:2.3.3.16)
Gene namesi
Name:gltA
Ordered Locus Names:Ta0169
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001024 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001699782 – 385Citrate synthaseAdd BLAST384

Proteomic databases

PRIDEiP21553.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi273075.Ta0169.

Structurei

Secondary structure

1385
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 11Combined sources3
Beta strandi15 – 25Combined sources11
Turni26 – 29Combined sources4
Beta strandi30 – 33Combined sources4
Helixi38 – 43Combined sources6
Helixi48 – 57Combined sources10
Helixi63 – 74Combined sources12
Helixi75 – 77Combined sources3
Helixi81 – 88Combined sources8
Helixi96 – 110Combined sources15
Turni118 – 120Combined sources3
Helixi121 – 143Combined sources23
Helixi157 – 166Combined sources10
Helixi172 – 184Combined sources13
Helixi192 – 201Combined sources10
Turni202 – 204Combined sources3
Helixi207 – 218Combined sources12
Turni221 – 223Combined sources3
Helixi226 – 237Combined sources12
Helixi240 – 242Combined sources3
Helixi243 – 250Combined sources8
Turni251 – 253Combined sources3
Beta strandi254 – 256Combined sources3
Helixi271 – 285Combined sources15
Helixi288 – 308Combined sources21
Helixi309 – 311Combined sources3
Turni317 – 320Combined sources4
Helixi321 – 328Combined sources8
Helixi333 – 335Combined sources3
Helixi336 – 360Combined sources25
Beta strandi367 – 370Combined sources4
Helixi381 – 383Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IFCX-ray1.70A/B/C/D1-385[»]
2R26X-ray2.50A/B/C/D2-385[»]
2R9EX-ray1.95A/B/C/D1-385[»]
4YBOX-ray2.18A/B/C/D2-385[»]
ProteinModelPortaliP21553.
SMRiP21553.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21553.

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Phylogenomic databases

eggNOGiarCOG04237. Archaea.
COG0372. LUCA.
HOGENOMiHOG000021225.
KOiK01647.
OMAiSAYHYMG.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR011278. 2-MeCitrate/Citrate_synth_II.
IPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01800. cit_synth_II. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21553-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPETEEISKG LEDVNIKWTR LTTIDGNKGI LRYGGYSVED IIASGAQDEE
60 70 80 90 100
IQYLFLYGNL PTEQELRKYK ETVQKGYKIP DFVINAIRQL PRESDAVAMQ
110 120 130 140 150
MAAVAAMAAS ETKFKWNKDT DRDVAAEMIG RMSAITVNVY RHIMNMPAEL
160 170 180 190 200
PKPSDSYAES FLNAAFGRKA TKEEIDAMNT ALILYTDHEV PASTTAGLVA
210 220 230 240 250
VSTLSDMYSG ITAALAALKG PLHGGAAEAA IAQFDEIKDP AMVEKWFNDN
260 270 280 290 300
IINGKKRLMG FGHRVYKTYD PRAKIFKGIA EKLSSKKPEV HKVYEIATKL
310 320 330 340 350
EDFGIKAFGS KGIYPNTDYF SGIVYMSIGF PLRNNIYTAL FALSRVTGWQ
360 370 380
AHFIEYVEEQ QRLIRPRAVY VGPAERKYVP IAERK
Length:385
Mass (Da):43,072
Last modified:January 23, 2007 - v2
Checksum:i9B7C2C88CEBCB690
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55282 Genomic DNA. Translation: CAA38996.1.
AL445063 Genomic DNA. Translation: CAC11315.1.
PIRiS13831. YKYT.
RefSeqiWP_010900596.1. NC_002578.1.

Genome annotation databases

EnsemblBacteriaiCAC11315; CAC11315; CAC11315.
GeneIDi1455814.
KEGGitac:Ta0169.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55282 Genomic DNA. Translation: CAA38996.1.
AL445063 Genomic DNA. Translation: CAC11315.1.
PIRiS13831. YKYT.
RefSeqiWP_010900596.1. NC_002578.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IFCX-ray1.70A/B/C/D1-385[»]
2R26X-ray2.50A/B/C/D2-385[»]
2R9EX-ray1.95A/B/C/D1-385[»]
4YBOX-ray2.18A/B/C/D2-385[»]
ProteinModelPortaliP21553.
SMRiP21553.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273075.Ta0169.

Proteomic databases

PRIDEiP21553.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC11315; CAC11315; CAC11315.
GeneIDi1455814.
KEGGitac:Ta0169.

Phylogenomic databases

eggNOGiarCOG04237. Archaea.
COG0372. LUCA.
HOGENOMiHOG000021225.
KOiK01647.
OMAiSAYHYMG.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.
BRENDAi2.3.3.16. 6324.

Miscellaneous databases

EvolutionaryTraceiP21553.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR011278. 2-MeCitrate/Citrate_synth_II.
IPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01800. cit_synth_II. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCISY_THEAC
AccessioniPrimary (citable) accession number: P21553
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.