Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein Wnt-2

Gene

Wnt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

GO - Molecular functioni

  1. cytokine activity Source: MGI
  2. frizzled binding Source: MGI

GO - Biological processi

  1. atrial cardiac muscle tissue morphogenesis Source: MGI
  2. canonical Wnt signaling pathway Source: MGI
  3. cardiac epithelial to mesenchymal transition Source: MGI
  4. cell-cell signaling Source: MGI
  5. cellular response to transforming growth factor beta stimulus Source: Ensembl
  6. labyrinthine layer blood vessel development Source: MGI
  7. lung development Source: Ensembl
  8. lung induction Source: MGI
  9. mammary gland epithelium development Source: Ensembl
  10. neuron differentiation Source: GO_Central
  11. positive regulation of canonical Wnt signaling pathway Source: MGI
  12. positive regulation of cardiac muscle cell proliferation Source: MGI
  13. positive regulation of cell proliferation Source: MGI
  14. positive regulation of endothelial cell proliferation Source: Ensembl
  15. positive regulation of epithelial cell proliferation involved in lung morphogenesis Source: MGI
  16. positive regulation of fibroblast proliferation Source: MGI
  17. positive regulation of mesenchymal cell proliferation Source: MGI
  18. positive regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  19. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  20. Wnt signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiREACT_295052. WNT ligand biogenesis and trafficking.
REACT_335449. Class B/2 (Secretin family receptors).

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-2
Alternative name(s):
INT-1-related protein
Short name:
IRP
Gene namesi
Name:Wnt2
Synonyms:Irp, Wnt-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:98954. Wnt2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. extracellular matrix Source: MGI
  3. extracellular space Source: GO_Central
  4. plasma membrane Source: Ensembl
  5. proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 360335Protein Wnt-2PRO_0000041411Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi76 ↔ 87By similarity
Disulfide bondi127 ↔ 135By similarity
Disulfide bondi137 ↔ 157By similarity
Disulfide bondi206 ↔ 220By similarity
Disulfide bondi208 ↔ 215By similarity
Lipidationi212 – 2121O-palmitoyl serine; by PORCNBy similarity
Disulfide bondi294 ↔ 309By similarity
Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi324 ↔ 339By similarity
Disulfide bondi326 ↔ 336By similarity
Disulfide bondi331 ↔ 332By similarity

Post-translational modificationi

Palmitoylation at Ser-212 is required for efficient binding to frizzled receptors. Palmitoylation is necessary for proper trafficking to cell surface (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP21552.
PRIDEiP21552.

PTM databases

PhosphoSiteiP21552.

Expressioni

Tissue specificityi

In embryos in the developing allantois, pericardium heart, and ventral-lateral mesoderm; in adults in lung, brain, heart and placenta.

Gene expression databases

BgeeiP21552.
CleanExiMM_WNT2.
ExpressionAtlasiP21552. baseline and differential.
GenevestigatoriP21552.

Interactioni

Protein-protein interaction databases

BioGridi204572. 1 interaction.
STRINGi10090.ENSMUSP00000010941.

Structurei

3D structure databases

ProteinModelPortaliP21552.
SMRiP21552. Positions 64-348.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG284879.
GeneTreeiENSGT00760000118943.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiP21552.
KOiK00182.
OMAiGYDTSRI.
OrthoDBiEOG7C8GJ8.
TreeFamiTF105310.

Family and domain databases

InterProiIPR005817. Wnt.
IPR009140. Wnt2.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01842. WNT2PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21552-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVPLGGIWL WLPLLLTWLT PEVSSSWWYM RATGGSSRVM CDNVPGLVSR
60 70 80 90 100
QRQLCHRHPD VMRAIGLGVA EWTAECQHQF RQHRWNCNTL DRDHSLFGRV
110 120 130 140 150
LLRSSRESAF VYAISSAGVV FAITRACSQG ELKSCSCDPK KKGSAKDSKG
160 170 180 190 200
TFDWGGCSDN IDYGIKFARA FVDAKERKGK DARALMNLHN NRAGRKAVKR
210 220 230 240 250
FLKQECKCHG VSGSCTLRTC WLAMADFRKT GDYLWRKYNG AIQVVMNQDG
260 270 280 290 300
TGFTVANKRF KKPTKNDLVY FENSPDYCIR DREAGSLGTA GRVCNLTSRG
310 320 330 340 350
MDSCEVMCCG RGYDTSHVTR MTKCECKFHW CCAVRCQDCL EALDVHTCKA
360
PKSADWATPT
Length:360
Mass (Da):40,482
Last modified:July 26, 2011 - v2
Checksum:i5E37644D3815EEAD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti232 – 2321D → E no nucleotide entry (PubMed:2693041).Curated
Sequence conflicti232 – 2321D → E no nucleotide entry (PubMed:2279700).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012093 mRNA. Translation: BAB28025.1.
CH466533 Genomic DNA. Translation: EDL13868.1.
BC026373 mRNA. Translation: AAH26373.1.
CCDSiCCDS19928.1.
PIRiB36470.
RefSeqiNP_076142.3. NM_023653.5.
UniGeneiMm.33653.

Genome annotation databases

EnsembliENSMUST00000010941; ENSMUSP00000010941; ENSMUSG00000010797.
GeneIDi22413.
KEGGimmu:22413.
UCSCiuc009bag.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012093 mRNA. Translation: BAB28025.1.
CH466533 Genomic DNA. Translation: EDL13868.1.
BC026373 mRNA. Translation: AAH26373.1.
CCDSiCCDS19928.1.
PIRiB36470.
RefSeqiNP_076142.3. NM_023653.5.
UniGeneiMm.33653.

3D structure databases

ProteinModelPortaliP21552.
SMRiP21552. Positions 64-348.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204572. 1 interaction.
STRINGi10090.ENSMUSP00000010941.

PTM databases

PhosphoSiteiP21552.

Proteomic databases

PaxDbiP21552.
PRIDEiP21552.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000010941; ENSMUSP00000010941; ENSMUSG00000010797.
GeneIDi22413.
KEGGimmu:22413.
UCSCiuc009bag.2. mouse.

Organism-specific databases

CTDi7472.
MGIiMGI:98954. Wnt2.

Phylogenomic databases

eggNOGiNOG284879.
GeneTreeiENSGT00760000118943.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiP21552.
KOiK00182.
OMAiGYDTSRI.
OrthoDBiEOG7C8GJ8.
TreeFamiTF105310.

Enzyme and pathway databases

ReactomeiREACT_295052. WNT ligand biogenesis and trafficking.
REACT_335449. Class B/2 (Secretin family receptors).

Miscellaneous databases

NextBioi302821.
PROiP21552.
SOURCEiSearch...

Gene expression databases

BgeeiP21552.
CleanExiMM_WNT2.
ExpressionAtlasiP21552. baseline and differential.
GenevestigatoriP21552.

Family and domain databases

InterProiIPR005817. Wnt.
IPR009140. Wnt2.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01842. WNT2PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence, chromosomal localization and developmental expression of the mouse int-1-related gene."
    McMahon J.A., McMahon A.P.
    Development 107:643-650(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development."
    Gavin B.J., McMahon J.A., McMahon A.P.
    Genes Dev. 4:2319-2332(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.

Entry informationi

Entry nameiWNT2_MOUSE
AccessioniPrimary (citable) accession number: P21552
Secondary accession number(s): Q9CZW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 1991
Last sequence update: July 26, 2011
Last modified: March 31, 2015
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.