P21542 (CARE_STRTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4''-mycarosyl isovaleryl-CoA transferase
EC=2.-.-.-

Gene names

Name:	carE
Synonyms:	acyB1

Organism

Streptomyces thermotolerans

Taxonomic identifier

80858 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function

Catalyzes the acylation of the mycaminose sugar during carbomycin biosynthesis. Recombinant strains containing care can use spiramycin as a substrate and convert it to the hybrid antibiotic, isovaleryl spiramycin.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Molecular function	Transferase
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	4''-mycarosyl isovaleryl-CoA transferase		PRO_0000089319

Sequences

	Sequence		Length	Mass (Da)
	P21542 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 88E322E488CBF5EC	FASTA	388	43,561
	10 20 30 40 50 60 MPLPKHLPAL GGMRFISALL VFTSHISTQP FFKNTEINSA LQFPLNRLGP LTVSFFFMLS 70 80 90 100 110 120 GFVLTWAGLP DKSKVNFWRR RTVRAYSLHL PVLLVTLLIV LALNEPNMGR SVWDGLLTNL 130 140 150 160 170 180 LLIQAWFPDH HEYGSMNPVA WSLSCELFFY AMFPFLFAFF TKVRTDRLWR WAAAVSVAAV 190 200 210 220 230 240 SIPLVALLLP ASPPLPWDPD MPQLRWWFIY MFPPVRLLEF VLGMLMAQIV IRGRWRGPRP 250 260 270 280 290 300 LACVALFSAV FAVTFAVPNH YDPGALTVPV IALLLASVAV GDVRGVRSWL GTRTMVLLGE 310 320 330 340 350 360 LTFAFYLVHY LIIQYGHRFA GGKQGYYRQW DTPAAVGLTL LAFTLALGLS AFLHFFVEKP 370 380 VMRTLGRPRR SPDAGSTPRS EPAPSGTP

References

[1]

"Production of a hybrid macrolide antibiotic in Streptomyces ambofaciens and Streptomyces lividans by introduction of a cloned carbomycin biosynthetic gene from Streptomyces thermotolerans." Epp J.K., Huber M.L.B., Turner J.R., Goodson T., Schoner B.E. Gene 85:293-301(1989) [PubMed: 2628170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 11416 / CBS 960.69 / DSM 40277 / JCM 4519 / NBRC 13088 / NRRL B-2345 / VKM Ac-1821.

[2]

"Cloning and nucleotide sequences of two genes involved in the 4''-O-acylation of macrolide antibiotics from Streptomyces thermotolerans." Arisawa A., Kawamura N., Tsunekawa H., Okamura K., Tone H., Okamoto R. Biosci. Biotechnol. Biochem. 57:2020-2025(1993) [PubMed: 7764361] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D31821 Genomic DNA. Translation: BAA06607.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002656. Acyl_transf_3. [Graphical view]
Pfam	PF01757. Acyl_transf_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARE_STRTH

Accession

Primary (citable) accession number: P21542

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	May 31, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program