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P21538

- REB1_YEAST

UniProt

P21538 - REB1_YEAST

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Protein

DNA-binding protein REB1

Gene
REB1, GRF2, YBR049C, YBR0502
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

DNA-binding protein that recognizes sites within both the enhancer and the promoter of rRNA transcription, as well as upstream of many genes transcribed by RNA polymerase II. It is essential for cell growth. May stimulate or inhibit transcription. Specifically recognizes the sequence 5'-CCGGGTA-3' or 5'-CGGGTRR-3' (where R is any purine). A member of the general regulatory factors (GRFs) which act as genome partitioners. Acts as a chromatin insulator which are known as STARs (Subtelomeric anti-silencing region). STARs prevent negative or positive transcription influence by extending across chromotin to a promoter.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi497 – 51923H-T-H motif By similarityAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: InterPro
  2. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. regulation of transcription from RNA polymerase II promoter Source: SGD
  2. termination of RNA polymerase I transcription Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29021-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein REB1
Alternative name(s):
QBP
Gene namesi
Name:REB1
Synonyms:GRF2
Ordered Locus Names:YBR049C
ORF Names:YBR0502
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR049c.
SGDiS000000253. REB1.

Subcellular locationi

GO - Cellular componenti

  1. nucleoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 810810DNA-binding protein REB1PRO_0000197089Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei355 – 3551Phosphoserine1 Publication
Cross-linki807 – 807Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP21538.
PaxDbiP21538.
PeptideAtlasiP21538.

Expressioni

Gene expression databases

GenevestigatoriP21538.

Interactioni

Protein-protein interaction databases

BioGridi32751. 57 interactions.
DIPiDIP-6374N.
IntActiP21538. 32 interactions.
MINTiMINT-614987.
STRINGi4932.YBR049C.

Structurei

3D structure databases

ProteinModelPortaliP21538.
SMRiP21538. Positions 402-575, 691-778.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini470 – 52354HTH myb-typeAdd
BLAST
Domaini692 – 71726Myb-likeAdd
BLAST

Sequence similaritiesi

Contains 1 Myb-like domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5147.
GeneTreeiENSGT00720000110079.
KOiK09424.
OMAiQNIVESS.
OrthoDBiEOG72NS0T.

Family and domain databases

Gene3Di1.10.10.60. 3 hits.
InterProiIPR009057. Homeodomain-like.
IPR017877. Myb-like_dom.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
[Graphical view]
SMARTiSM00717. SANT. 4 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS51294. HTH_MYB. 1 hit.
PS50090. MYB_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21538-1 [UniParc]FASTAAdd to Basket

« Hide

MPSGHNDKNA NQESVEEAVL KYVGVGLDHQ NHDPQLHTKD LENKHSKKQN    50
IVESSSDVDV NNNDDSNRNE DNNDDSENIS ALNANESSSN VDHANSNEQH 100
NAVMDWYLRQ TAHNQQDDED DENNNNTDNG NDSNNHFSQS DIVVDDDDDK 150
NKKDAGVGVD DDHQSMAMAA VAAAYTLSKN NNNNNSIAND SNSRKRQHDN 200
GNNHENSQKK RKNNNDDDDR QIGNVDPELT TLGDADDNDT NNDVIDRDQL 250
VHKAIIDADS ITQHPDFQQY LNTAADTDDN EKLKHIKDHL MRTHGLNHQN 300
KNHNDDTDDL SNSTKQYSEL QKDSMLDSSL NKSRNYMEVL PKVISQDTQP 350
HQQKSPSHDN EAGSVDNSEI SQLLQSAATK ASSLVSLSSS SATPSTSRSN 400
NSKAFDKAED AALERFINEY EAIERLTRQQ VCERIWSSDR PKDNFWNNIY 450
KVLPYRSSSS IYKHMRRKYH IFEQRGKWTA EEEQELAKLC AEKEGQWAEI 500
GKTLGRMPED CRDRWRNYVK CGTNRASNRW SVEEEELLKK VISDMLEEAQ 550
QQQSQLHPNL LEEEQHLLQD DQNDHRNNDE DDDDTASAAA AAAAAIQEQQ 600
QLLQQKQQDD DDAIAAAAAA ASSSLGDNKD EDKPHDSLGI QLDDNSQNSM 650
VPAPSATSTH SKSLSNTIRR HNNKLRKSLM GNGKLDFKDI INWTIVSERM 700
GGTRSRIQCR YKWNKLVKRE AIAKIQTVKD DDMLWIFEKL RDLGITEDSQ 750
VDWDELAALK PGMKLNGLEL KLCYERMKKK VKGYKQKSIN EISKELVDYF 800
SSNISMKTEN 810
Length:810
Mass (Da):91,874
Last modified:October 1, 1994 - v2
Checksum:i318EB17D20D004B6
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 561S → N in AAA34963. 1 Publication
Sequence conflicti592 – 61827AAAAI…IAAAA → RAVVFKNNNNFFNKSSKMMT MLLRSC in AAA34963. 1 PublicationAdd
BLAST
Sequence conflicti627 – 6271D → E in AAA34963. 1 Publication
Sequence conflicti636 – 6361D → E in AAA34963. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58728 Genomic DNA. Translation: AAA34963.1.
Z35918 Genomic DNA. Translation: CAA84992.1.
Z46260 Genomic DNA. Translation: CAA86391.1.
BK006936 Genomic DNA. Translation: DAA07169.1.
PIRiS45907.
RefSeqiNP_009605.1. NM_001178397.1.

Genome annotation databases

EnsemblFungiiYBR049C; YBR049C; YBR049C.
GeneIDi852338.
KEGGisce:YBR049C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58728 Genomic DNA. Translation: AAA34963.1 .
Z35918 Genomic DNA. Translation: CAA84992.1 .
Z46260 Genomic DNA. Translation: CAA86391.1 .
BK006936 Genomic DNA. Translation: DAA07169.1 .
PIRi S45907.
RefSeqi NP_009605.1. NM_001178397.1.

3D structure databases

ProteinModelPortali P21538.
SMRi P21538. Positions 402-575, 691-778.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32751. 57 interactions.
DIPi DIP-6374N.
IntActi P21538. 32 interactions.
MINTi MINT-614987.
STRINGi 4932.YBR049C.

Proteomic databases

MaxQBi P21538.
PaxDbi P21538.
PeptideAtlasi P21538.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR049C ; YBR049C ; YBR049C .
GeneIDi 852338.
KEGGi sce:YBR049C.

Organism-specific databases

CYGDi YBR049c.
SGDi S000000253. REB1.

Phylogenomic databases

eggNOGi COG5147.
GeneTreei ENSGT00720000110079.
KOi K09424.
OMAi QNIVESS.
OrthoDBi EOG72NS0T.

Enzyme and pathway databases

BioCyci YEAST:G3O-29021-MONOMER.

Miscellaneous databases

NextBioi 971067.
PROi P21538.

Gene expression databases

Genevestigatori P21538.

Family and domain databases

Gene3Di 1.10.10.60. 3 hits.
InterProi IPR009057. Homeodomain-like.
IPR017877. Myb-like_dom.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
[Graphical view ]
SMARTi SM00717. SANT. 4 hits.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 2 hits.
PROSITEi PS51294. HTH_MYB. 1 hit.
PS50090. MYB_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "REB1, a yeast DNA-binding protein with many targets, is essential for growth and bears some resemblance to the oncogene myb."
    Ju Q., Morrow B.E., Warner J.R.
    Mol. Cell. Biol. 10:5226-5234(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Sequence and analysis of 24 kb on chromosome II of Saccharomyces cerevisiae."
    Aljinovic G., Pohl T.M.
    Yeast 11:475-479(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "A proteomic strategy for gaining insights into protein sumoylation in yeast."
    Denison C., Rudner A.D., Gerber S.A., Bakalarski C.E., Moazed D., Gygi S.P.
    Mol. Cell. Proteomics 4:246-254(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 795-810, SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-807.
    Strain: EJY251-11b.
  6. "An activation-independent role of transcription factors in insulator function."
    Fourel G., Boscheron C., Revardel E., Lebrun E., Hu Y.-F., Simmen K.C., Mueller K., Li R., Mermod N., Gilson E.
    EMBO Rep. 2:124-132(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "General regulatory factors (GRFs) as genome partitioners."
    Fourel G., Miyake T., Defossez P.-A., Li R., Gilson E.
    J. Biol. Chem. 277:41736-41743(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-355, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiREB1_YEAST
AccessioniPrimary (citable) accession number: P21538
Secondary accession number(s): D6VQ49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: October 1, 1994
Last modified: May 14, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7510 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

Similar proteinsi