P21538 (REB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-binding protein REB1

Alternative name(s):
QBP

Gene names

Name:	REB1
Synonyms:	GRF2
Ordered Locus Names:	YBR049C
ORF Names:	YBR0502

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	810 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-binding protein that recognizes sites within both the enhancer and the promoter of rRNA transcription, as well as upstream of many genes transcribed by RNA polymerase II. It is essential for cell growth. May stimulate or inhibit transcription. Specifically recognizes the sequence 5'-CCGGGTA-3' or 5'-CGGGTRR-3' (where R is any purine). A member of the general regulatory factors (GRFs) which act as genome partitioners. Acts as a chromatin insulator which are known as STARs (Subtelomeric anti-silencing region). STARs prevent negative or positive transcription influence by extending across chromotin to a promoter. Ref.6 Ref.7
Subcellular location	Nucleus.
Miscellaneous	Present with 7510 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 HTH myb-type DNA-binding domain. Contains 1 Myb-like domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Ligand	DNA-binding
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription from RNA polymerase II promoter Traceable author statement PubMed 9632820. Source: SGD termination of RNA polymerase I transcription Traceable author statement PubMed 7565745. Source: SGD
Cellular_component	nucleoplasm Inferred from direct assay PubMed 20852627. Source: SGD
Molecular_function	chromatin binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from direct assay PubMed 19111667. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 810	810	DNA-binding protein REB1		PRO_0000197089
Regions
	Domain	470 – 523	54	HTH myb-type
	Domain	692 – 717	26	Myb-like
	DNA binding	497 – 519	23	H-T-H motif By similarity
Amino acid modifications
	Modified residue	355	1	Phosphoserine Ref.10 Ref.11 Ref.12
	Modified residue	357	1	Phosphoserine Ref.9
	Modified residue	383	1	Phosphoserine Ref.12
	Modified residue	386	1	Phosphoserine Ref.12
	Cross-link	807		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Ref.5
Experimental info
	Sequence conflict	56	1	S → N in AAA34963. Ref.1
	Sequence conflict	592 – 618	27	AAAAI…IAAAA → RAVVFKNNNNFFNKSSKMMT MLLRSC in AAA34963. Ref.1
	Sequence conflict	627	1	D → E in AAA34963. Ref.1
	Sequence conflict	636	1	D → E in AAA34963. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P21538 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 318EB17D20D004B6	FASTA	810	91,874
	10 20 30 40 50 60 MPSGHNDKNA NQESVEEAVL KYVGVGLDHQ NHDPQLHTKD LENKHSKKQN IVESSSDVDV 70 80 90 100 110 120 NNNDDSNRNE DNNDDSENIS ALNANESSSN VDHANSNEQH NAVMDWYLRQ TAHNQQDDED 130 140 150 160 170 180 DENNNNTDNG NDSNNHFSQS DIVVDDDDDK NKKDAGVGVD DDHQSMAMAA VAAAYTLSKN 190 200 210 220 230 240 NNNNNSIAND SNSRKRQHDN GNNHENSQKK RKNNNDDDDR QIGNVDPELT TLGDADDNDT 250 260 270 280 290 300 NNDVIDRDQL VHKAIIDADS ITQHPDFQQY LNTAADTDDN EKLKHIKDHL MRTHGLNHQN 310 320 330 340 350 360 KNHNDDTDDL SNSTKQYSEL QKDSMLDSSL NKSRNYMEVL PKVISQDTQP HQQKSPSHDN 370 380 390 400 410 420 EAGSVDNSEI SQLLQSAATK ASSLVSLSSS SATPSTSRSN NSKAFDKAED AALERFINEY 430 440 450 460 470 480 EAIERLTRQQ VCERIWSSDR PKDNFWNNIY KVLPYRSSSS IYKHMRRKYH IFEQRGKWTA 490 500 510 520 530 540 EEEQELAKLC AEKEGQWAEI GKTLGRMPED CRDRWRNYVK CGTNRASNRW SVEEEELLKK 550 560 570 580 590 600 VISDMLEEAQ QQQSQLHPNL LEEEQHLLQD DQNDHRNNDE DDDDTASAAA AAAAAIQEQQ 610 620 630 640 650 660 QLLQQKQQDD DDAIAAAAAA ASSSLGDNKD EDKPHDSLGI QLDDNSQNSM VPAPSATSTH 670 680 690 700 710 720 SKSLSNTIRR HNNKLRKSLM GNGKLDFKDI INWTIVSERM GGTRSRIQCR YKWNKLVKRE 730 740 750 760 770 780 AIAKIQTVKD DDMLWIFEKL RDLGITEDSQ VDWDELAALK PGMKLNGLEL KLCYERMKKK 790 800 810 VKGYKQKSIN EISKELVDYF SSNISMKTEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"REB1, a yeast DNA-binding protein with many targets, is essential for growth and bears some resemblance to the oncogene myb." Ju Q., Morrow B.E., Warner J.R. Mol. Cell. Biol. 10:5226-5234(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Sequence and analysis of 24 kb on chromosome II of Saccharomyces cerevisiae." Aljinovic G., Pohl T.M. Yeast 11:475-479(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"A proteomic strategy for gaining insights into protein sumoylation in yeast." Denison C., Rudner A.D., Gerber S.A., Bakalarski C.E., Moazed D., Gygi S.P. Mol. Cell. Proteomics 4:246-254(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 795-810, SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-807, MASS SPECTROMETRY. Strain: EJY251-11b.
[6]	"An activation-independent role of transcription factors in insulator function." Fourel G., Boscheron C., Revardel E., Lebrun E., Hu Y.-F., Simmen K.C., Mueller K., Li R., Mermod N., Gilson E. EMBO Rep. 2:124-132(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"General regulatory factors (GRFs) as genome partitioners." Fourel G., Miyake T., Defossez P.-A., Li R., Gilson E. J. Biol. Chem. 277:41736-41743(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-357, MASS SPECTROMETRY. Strain: YAL6B.
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-355, MASS SPECTROMETRY. Strain: ADR376.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-355, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-355; SER-383 AND SER-386, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M58728 Genomic DNA. Translation: AAA34963.1. Z35918 Genomic DNA. Translation: CAA84992.1. Z46260 Genomic DNA. Translation: CAA86391.1. BK006936 Genomic DNA. Translation: DAA07169.1.
PIR	S45907.
RefSeq	NP_009605.1. NM_001178397.1.
3D structure databases
ProteinModelPortal	P21538.
SMR	P21538. Positions 474-518.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6374N.
IntAct	P21538. 32 interactions.
MINT	MINT-614987.
STRING	4932.YBR049C.
Proteomic databases
PaxDb	P21538.
PeptideAtlas	P21538.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR049C; YBR049C; YBR049C.
GeneID	852338.
KEGG	sce:YBR049C.
Organism-specific databases
CYGD	YBR049c.
SGD	S000000253. REB1.
Phylogenomic databases
eggNOG	COG5147.
GeneTree	ENSGT00530000063659.
KO	K09424.
OMA	SHEDASH.
Gene expression databases
Genevestigator	P21538.
GermOnline	YBR049C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.10.60. 3 hits.
InterPro	IPR009057. Homeodomain-like. IPR017877. Myb-like_dom. IPR017930. Myb_dom. IPR001005. SANT/Myb. [Graphical view]
SMART	SM00717. SANT. 4 hits. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 2 hits.
PROSITE	PS51294. HTH_MYB. 1 hit. PS50090. MYB_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971067.

Entry information

Entry name

REB1_YEAST

Accession

Primary (citable) accession number: P21538
Secondary accession number(s): D6VQ49

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 125 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program