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P21538

- REB1_YEAST

UniProt

P21538 - REB1_YEAST

Protein

DNA-binding protein REB1

Gene

REB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    DNA-binding protein that recognizes sites within both the enhancer and the promoter of rRNA transcription, as well as upstream of many genes transcribed by RNA polymerase II. It is essential for cell growth. May stimulate or inhibit transcription. Specifically recognizes the sequence 5'-CCGGGTA-3' or 5'-CGGGTRR-3' (where R is any purine). A member of the general regulatory factors (GRFs) which act as genome partitioners. Acts as a chromatin insulator which are known as STARs (Subtelomeric anti-silencing region). STARs prevent negative or positive transcription influence by extending across chromotin to a promoter.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi497 – 51923H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: InterPro
    2. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. regulation of transcription from RNA polymerase II promoter Source: SGD
    2. termination of RNA polymerase I transcription Source: SGD

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29021-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-binding protein REB1
    Alternative name(s):
    QBP
    Gene namesi
    Name:REB1
    Synonyms:GRF2
    Ordered Locus Names:YBR049C
    ORF Names:YBR0502
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR049c.
    SGDiS000000253. REB1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleoplasm Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 810810DNA-binding protein REB1PRO_0000197089Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei355 – 3551Phosphoserine1 Publication
    Cross-linki807 – 807Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP21538.
    PaxDbiP21538.
    PeptideAtlasiP21538.

    Expressioni

    Gene expression databases

    GenevestigatoriP21538.

    Interactioni

    Protein-protein interaction databases

    BioGridi32751. 57 interactions.
    DIPiDIP-6374N.
    IntActiP21538. 32 interactions.
    MINTiMINT-614987.
    STRINGi4932.YBR049C.

    Structurei

    3D structure databases

    ProteinModelPortaliP21538.
    SMRiP21538. Positions 402-575, 691-778.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini470 – 52354HTH myb-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini692 – 71726Myb-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 HTH myb-type DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 Myb-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5147.
    GeneTreeiENSGT00720000110079.
    KOiK09424.
    OMAiQNIVESS.
    OrthoDBiEOG72NS0T.

    Family and domain databases

    Gene3Di1.10.10.60. 3 hits.
    InterProiIPR009057. Homeodomain-like.
    IPR017877. Myb-like_dom.
    IPR017930. Myb_dom.
    IPR001005. SANT/Myb.
    [Graphical view]
    SMARTiSM00717. SANT. 4 hits.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 2 hits.
    PROSITEiPS51294. HTH_MYB. 1 hit.
    PS50090. MYB_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21538-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSGHNDKNA NQESVEEAVL KYVGVGLDHQ NHDPQLHTKD LENKHSKKQN    50
    IVESSSDVDV NNNDDSNRNE DNNDDSENIS ALNANESSSN VDHANSNEQH 100
    NAVMDWYLRQ TAHNQQDDED DENNNNTDNG NDSNNHFSQS DIVVDDDDDK 150
    NKKDAGVGVD DDHQSMAMAA VAAAYTLSKN NNNNNSIAND SNSRKRQHDN 200
    GNNHENSQKK RKNNNDDDDR QIGNVDPELT TLGDADDNDT NNDVIDRDQL 250
    VHKAIIDADS ITQHPDFQQY LNTAADTDDN EKLKHIKDHL MRTHGLNHQN 300
    KNHNDDTDDL SNSTKQYSEL QKDSMLDSSL NKSRNYMEVL PKVISQDTQP 350
    HQQKSPSHDN EAGSVDNSEI SQLLQSAATK ASSLVSLSSS SATPSTSRSN 400
    NSKAFDKAED AALERFINEY EAIERLTRQQ VCERIWSSDR PKDNFWNNIY 450
    KVLPYRSSSS IYKHMRRKYH IFEQRGKWTA EEEQELAKLC AEKEGQWAEI 500
    GKTLGRMPED CRDRWRNYVK CGTNRASNRW SVEEEELLKK VISDMLEEAQ 550
    QQQSQLHPNL LEEEQHLLQD DQNDHRNNDE DDDDTASAAA AAAAAIQEQQ 600
    QLLQQKQQDD DDAIAAAAAA ASSSLGDNKD EDKPHDSLGI QLDDNSQNSM 650
    VPAPSATSTH SKSLSNTIRR HNNKLRKSLM GNGKLDFKDI INWTIVSERM 700
    GGTRSRIQCR YKWNKLVKRE AIAKIQTVKD DDMLWIFEKL RDLGITEDSQ 750
    VDWDELAALK PGMKLNGLEL KLCYERMKKK VKGYKQKSIN EISKELVDYF 800
    SSNISMKTEN 810
    Length:810
    Mass (Da):91,874
    Last modified:October 1, 1994 - v2
    Checksum:i318EB17D20D004B6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti56 – 561S → N in AAA34963. (PubMed:2204808)Curated
    Sequence conflicti592 – 61827AAAAI…IAAAA → RAVVFKNNNNFFNKSSKMMT MLLRSC in AAA34963. (PubMed:2204808)CuratedAdd
    BLAST
    Sequence conflicti627 – 6271D → E in AAA34963. (PubMed:2204808)Curated
    Sequence conflicti636 – 6361D → E in AAA34963. (PubMed:2204808)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58728 Genomic DNA. Translation: AAA34963.1.
    Z35918 Genomic DNA. Translation: CAA84992.1.
    Z46260 Genomic DNA. Translation: CAA86391.1.
    BK006936 Genomic DNA. Translation: DAA07169.1.
    PIRiS45907.
    RefSeqiNP_009605.1. NM_001178397.1.

    Genome annotation databases

    EnsemblFungiiYBR049C; YBR049C; YBR049C.
    GeneIDi852338.
    KEGGisce:YBR049C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58728 Genomic DNA. Translation: AAA34963.1 .
    Z35918 Genomic DNA. Translation: CAA84992.1 .
    Z46260 Genomic DNA. Translation: CAA86391.1 .
    BK006936 Genomic DNA. Translation: DAA07169.1 .
    PIRi S45907.
    RefSeqi NP_009605.1. NM_001178397.1.

    3D structure databases

    ProteinModelPortali P21538.
    SMRi P21538. Positions 402-575, 691-778.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32751. 57 interactions.
    DIPi DIP-6374N.
    IntActi P21538. 32 interactions.
    MINTi MINT-614987.
    STRINGi 4932.YBR049C.

    Proteomic databases

    MaxQBi P21538.
    PaxDbi P21538.
    PeptideAtlasi P21538.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR049C ; YBR049C ; YBR049C .
    GeneIDi 852338.
    KEGGi sce:YBR049C.

    Organism-specific databases

    CYGDi YBR049c.
    SGDi S000000253. REB1.

    Phylogenomic databases

    eggNOGi COG5147.
    GeneTreei ENSGT00720000110079.
    KOi K09424.
    OMAi QNIVESS.
    OrthoDBi EOG72NS0T.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29021-MONOMER.

    Miscellaneous databases

    NextBioi 971067.
    PROi P21538.

    Gene expression databases

    Genevestigatori P21538.

    Family and domain databases

    Gene3Di 1.10.10.60. 3 hits.
    InterProi IPR009057. Homeodomain-like.
    IPR017877. Myb-like_dom.
    IPR017930. Myb_dom.
    IPR001005. SANT/Myb.
    [Graphical view ]
    SMARTi SM00717. SANT. 4 hits.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 2 hits.
    PROSITEi PS51294. HTH_MYB. 1 hit.
    PS50090. MYB_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "REB1, a yeast DNA-binding protein with many targets, is essential for growth and bears some resemblance to the oncogene myb."
      Ju Q., Morrow B.E., Warner J.R.
      Mol. Cell. Biol. 10:5226-5234(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Sequence and analysis of 24 kb on chromosome II of Saccharomyces cerevisiae."
      Aljinovic G., Pohl T.M.
      Yeast 11:475-479(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "A proteomic strategy for gaining insights into protein sumoylation in yeast."
      Denison C., Rudner A.D., Gerber S.A., Bakalarski C.E., Moazed D., Gygi S.P.
      Mol. Cell. Proteomics 4:246-254(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 795-810, SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-807.
      Strain: EJY251-11b.
    6. "An activation-independent role of transcription factors in insulator function."
      Fourel G., Boscheron C., Revardel E., Lebrun E., Hu Y.-F., Simmen K.C., Mueller K., Li R., Mermod N., Gilson E.
      EMBO Rep. 2:124-132(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "General regulatory factors (GRFs) as genome partitioners."
      Fourel G., Miyake T., Defossez P.-A., Li R., Gilson E.
      J. Biol. Chem. 277:41736-41743(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-355, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiREB1_YEAST
    AccessioniPrimary (citable) accession number: P21538
    Secondary accession number(s): D6VQ49
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7510 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3