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P21538 (REB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-binding protein REB1
Alternative name(s):
QBP
Gene names
Name:REB1
Synonyms:GRF2
Ordered Locus Names:YBR049C
ORF Names:YBR0502
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length810 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-binding protein that recognizes sites within both the enhancer and the promoter of rRNA transcription, as well as upstream of many genes transcribed by RNA polymerase II. It is essential for cell growth. May stimulate or inhibit transcription. Specifically recognizes the sequence 5'-CCGGGTA-3' or 5'-CGGGTRR-3' (where R is any purine). A member of the general regulatory factors (GRFs) which act as genome partitioners. Acts as a chromatin insulator which are known as STARs (Subtelomeric anti-silencing region). STARs prevent negative or positive transcription influence by extending across chromotin to a promoter. Ref.6 Ref.7

Subcellular location

Nucleus.

Miscellaneous

Present with 7510 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 HTH myb-type DNA-binding domain.

Contains 1 Myb-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 810810DNA-binding protein REB1
PRO_0000197089

Regions

Domain470 – 52354HTH myb-type
Domain692 – 71726Myb-like
DNA binding497 – 51923H-T-H motif By similarity

Amino acid modifications

Modified residue3551Phosphoserine Ref.9
Cross-link807Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Ref.5

Experimental info

Sequence conflict561S → N in AAA34963. Ref.1
Sequence conflict592 – 61827AAAAI…IAAAA → RAVVFKNNNNFFNKSSKMMT MLLRSC in AAA34963. Ref.1
Sequence conflict6271D → E in AAA34963. Ref.1
Sequence conflict6361D → E in AAA34963. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P21538 [UniParc].

Last modified October 1, 1994. Version 2.
Checksum: 318EB17D20D004B6

FASTA81091,874
        10         20         30         40         50         60 
MPSGHNDKNA NQESVEEAVL KYVGVGLDHQ NHDPQLHTKD LENKHSKKQN IVESSSDVDV 

        70         80         90        100        110        120 
NNNDDSNRNE DNNDDSENIS ALNANESSSN VDHANSNEQH NAVMDWYLRQ TAHNQQDDED 

       130        140        150        160        170        180 
DENNNNTDNG NDSNNHFSQS DIVVDDDDDK NKKDAGVGVD DDHQSMAMAA VAAAYTLSKN 

       190        200        210        220        230        240 
NNNNNSIAND SNSRKRQHDN GNNHENSQKK RKNNNDDDDR QIGNVDPELT TLGDADDNDT 

       250        260        270        280        290        300 
NNDVIDRDQL VHKAIIDADS ITQHPDFQQY LNTAADTDDN EKLKHIKDHL MRTHGLNHQN 

       310        320        330        340        350        360 
KNHNDDTDDL SNSTKQYSEL QKDSMLDSSL NKSRNYMEVL PKVISQDTQP HQQKSPSHDN 

       370        380        390        400        410        420 
EAGSVDNSEI SQLLQSAATK ASSLVSLSSS SATPSTSRSN NSKAFDKAED AALERFINEY 

       430        440        450        460        470        480 
EAIERLTRQQ VCERIWSSDR PKDNFWNNIY KVLPYRSSSS IYKHMRRKYH IFEQRGKWTA 

       490        500        510        520        530        540 
EEEQELAKLC AEKEGQWAEI GKTLGRMPED CRDRWRNYVK CGTNRASNRW SVEEEELLKK 

       550        560        570        580        590        600 
VISDMLEEAQ QQQSQLHPNL LEEEQHLLQD DQNDHRNNDE DDDDTASAAA AAAAAIQEQQ 

       610        620        630        640        650        660 
QLLQQKQQDD DDAIAAAAAA ASSSLGDNKD EDKPHDSLGI QLDDNSQNSM VPAPSATSTH 

       670        680        690        700        710        720 
SKSLSNTIRR HNNKLRKSLM GNGKLDFKDI INWTIVSERM GGTRSRIQCR YKWNKLVKRE 

       730        740        750        760        770        780 
AIAKIQTVKD DDMLWIFEKL RDLGITEDSQ VDWDELAALK PGMKLNGLEL KLCYERMKKK 

       790        800        810 
VKGYKQKSIN EISKELVDYF SSNISMKTEN 

« Hide

References

« Hide 'large scale' references
[1]"REB1, a yeast DNA-binding protein with many targets, is essential for growth and bears some resemblance to the oncogene myb."
Ju Q., Morrow B.E., Warner J.R.
Mol. Cell. Biol. 10:5226-5234(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Sequence and analysis of 24 kb on chromosome II of Saccharomyces cerevisiae."
Aljinovic G., Pohl T.M.
Yeast 11:475-479(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"A proteomic strategy for gaining insights into protein sumoylation in yeast."
Denison C., Rudner A.D., Gerber S.A., Bakalarski C.E., Moazed D., Gygi S.P.
Mol. Cell. Proteomics 4:246-254(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 795-810, SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-807.
Strain: EJY251-11b.
[6]"An activation-independent role of transcription factors in insulator function."
Fourel G., Boscheron C., Revardel E., Lebrun E., Hu Y.-F., Simmen K.C., Mueller K., Li R., Mermod N., Gilson E.
EMBO Rep. 2:124-132(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"General regulatory factors (GRFs) as genome partitioners."
Fourel G., Miyake T., Defossez P.-A., Li R., Gilson E.
J. Biol. Chem. 277:41736-41743(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-355, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M58728 Genomic DNA. Translation: AAA34963.1.
Z35918 Genomic DNA. Translation: CAA84992.1.
Z46260 Genomic DNA. Translation: CAA86391.1.
BK006936 Genomic DNA. Translation: DAA07169.1.
PIRS45907.
RefSeqNP_009605.1. NM_001178397.1.

3D structure databases

ProteinModelPortalP21538.
SMRP21538. Positions 402-575, 691-778.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32751. 57 interactions.
DIPDIP-6374N.
IntActP21538. 32 interactions.
MINTMINT-614987.
STRING4932.YBR049C.

Proteomic databases

PaxDbP21538.
PeptideAtlasP21538.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR049C; YBR049C; YBR049C.
GeneID852338.
KEGGsce:YBR049C.

Organism-specific databases

CYGDYBR049c.
SGDS000000253. REB1.

Phylogenomic databases

eggNOGCOG5147.
GeneTreeENSGT00720000110079.
KOK09424.
OMAQNIVESS.
OrthoDBEOG72NS0T.

Enzyme and pathway databases

BioCycYEAST:G3O-29021-MONOMER.

Gene expression databases

GenevestigatorP21538.

Family and domain databases

Gene3D1.10.10.60. 3 hits.
InterProIPR009057. Homeodomain-like.
IPR017877. Myb-like_dom.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
[Graphical view]
SMARTSM00717. SANT. 4 hits.
[Graphical view]
SUPFAMSSF46689. SSF46689. 2 hits.
PROSITEPS51294. HTH_MYB. 1 hit.
PS50090. MYB_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio971067.
PROP21538.

Entry information

Entry nameREB1_YEAST
AccessionPrimary (citable) accession number: P21538
Secondary accession number(s): D6VQ49
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: October 1, 1994
Last modified: March 19, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families