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Protein

Serine carboxypeptidase 3

Gene

CBP3

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Release of a C-terminal amino acid with broad specificity.PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by mercuric ions.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei223 – 2231By similarity
Active sitei411 – 4111By similarity
Binding sitei414 – 4141Substrate
Active sitei468 – 4681By similarity

GO - Molecular functioni

  1. serine-type carboxypeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Protein family/group databases

MEROPSiS10.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine carboxypeptidase 3 (EC:3.4.16.5)
Alternative name(s):
CP-MIII
Serine carboxypeptidase III
Gene namesi
Name:CBP3
Synonyms:CXP;3
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiP21529.

Subcellular locationi

Secreted
Note: Secreted into the endosperm.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Propeptidei20 – 80611 PublicationPRO_0000004324Add
BLAST
Chaini81 – 491411Serine carboxypeptidase 3PRO_0000004325Add
BLAST
Propeptidei492 – 50817PRO_0000004326Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei81 – 811Blocked amino end (Leu)
Disulfide bondi133 ↔ 373By similarity
Glycosylationi151 – 1511N-linked (GlcNAc...)
Disulfide bondi301 ↔ 316By similarity
Disulfide bondi339 ↔ 344By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Developmental stagei

Expressed mainly in the aleurone and, to a lesser extent in the embryo, throughout the 5-days germination period exclusively, with a maximal level at 3 days. Also found in the roots and shoots of the growing seedling.

Gene expression databases

ExpressionAtlasiP21529. baseline.
GenevestigatoriP21529.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP21529.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21529-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVTTPRLVSL LLLLALCAAA AGALRLPPDA SFPGAQAERL IRALNLLPKD
60 70 80 90 100
SSSSSGRHGA RVGEGNEDVA PGQLLERRVT LPGLPEGVAD LGHHAGYYRL
110 120 130 140 150
PNTHDARMFY FFFESRGKKE DPVVIWLTGG PGCSSELAVF YENGPFTIAN
160 170 180 190 200
NMSLVWNKFG WDKISNIIFV DQPTGTGFSY SSDDRDTRHD ETGVSNDLYD
210 220 230 240 250
FLQVFFKKHP EFIKNDFFIT GESYAGHYIP AFASRVHQGN KKNEGTHINL
260 270 280 290 300
KGFAIGNGLT DPAIQYKAYT DYALEMNLIQ KADYERINKF IPPCEFAIKL
310 320 330 340 350
CGTNGKASCM AAYMVCNTIF NSIMKLVGTK NYYDVRKECE GKLCYDFSNL
360 370 380 390 400
EKFFGDKAVR QAIGVGDIEF VSCSTSVYQA MLTDWMRNLE VGIPALLEDG
410 420 430 440 450
INVLIYAGEY DLICNWLGNS RWVHSMEWSG QKDFAKTAES SFLVDDAQAG
460 470 480 490 500
VLKSHGALSF LKVHNAGHMV PMDQPKAALE MLRRFTQGKL KEAVPEEESS

TTSFYAAM
Length:508
Mass (Da):56,362
Last modified:July 15, 1998 - v2
Checksum:i70C6751D78D40AB6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti265 – 2651Q → V Polymorphism.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09604 mRNA. Translation: CAA70817.1.
PIRiA35275.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09604 mRNA. Translation: CAA70817.1.
PIRiA35275.

3D structure databases

ProteinModelPortaliP21529.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS10.009.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GrameneiP21529.

Gene expression databases

ExpressionAtlasiP21529. baseline.
GenevestigatoriP21529.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Rocher A., Lok F., Cameron-Mills V., von Wettstein D.
    Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Himalaya.
    Tissue: Aleurone.
  2. "Primary structure of carboxypeptidase III from malted barley."
    Soerensen S.B., Svendsen I., Breddam K.
    Carlsberg Res. Commun. 54:193-202(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 81-491.
    Strain: cv. Gula.

Entry informationi

Entry nameiCBP3_HORVU
AccessioniPrimary (citable) accession number: P21529
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: July 15, 1998
Last modified: January 7, 2015
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.