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P21529 (CBP3_HORVU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine carboxypeptidase 3

EC=3.4.16.5
Alternative name(s):
CP-MIII
Serine carboxypeptidase III
Gene names
Name:CBP3
Synonyms:CXP;3
OrganismHordeum vulgare (Barley)
Taxonomic identifier4513 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Protein attributes

Sequence length508 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Release of a C-terminal amino acid with broad specificity.

Enzyme regulation

Inhibited by mercuric ions.

Subunit structure

Monomer.

Subcellular location

Secreted. Note: Secreted into the endosperm.

Developmental stage

Expressed mainly in the aleurone and, to a lesser extent in the embryo, throughout the 5-days germination period exclusively, with a maximal level at 3 days. Also found in the roots and shoots of the growing seedling.

Sequence similarities

Belongs to the peptidase S10 family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionCarboxypeptidase
Hydrolase
Protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type carboxypeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Propeptide20 – 8061
PRO_0000004324
Chain81 – 491411Serine carboxypeptidase 3
PRO_0000004325
Propeptide492 – 50817
PRO_0000004326

Sites

Active site2231 By similarity
Active site4111 By similarity
Active site4681 By similarity
Binding site4141Substrate

Amino acid modifications

Modified residue811Blocked amino end (Leu)
Glycosylation1511N-linked (GlcNAc...)
Disulfide bond133 ↔ 373 By similarity
Disulfide bond301 ↔ 316 By similarity
Disulfide bond339 ↔ 344 By similarity

Natural variations

Natural variant2651Q → V Polymorphism.

Sequences

Sequence LengthMass (Da)Tools
P21529 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: 70C6751D78D40AB6

FASTA50856,362
        10         20         30         40         50         60 
MVTTPRLVSL LLLLALCAAA AGALRLPPDA SFPGAQAERL IRALNLLPKD SSSSSGRHGA 

        70         80         90        100        110        120 
RVGEGNEDVA PGQLLERRVT LPGLPEGVAD LGHHAGYYRL PNTHDARMFY FFFESRGKKE 

       130        140        150        160        170        180 
DPVVIWLTGG PGCSSELAVF YENGPFTIAN NMSLVWNKFG WDKISNIIFV DQPTGTGFSY 

       190        200        210        220        230        240 
SSDDRDTRHD ETGVSNDLYD FLQVFFKKHP EFIKNDFFIT GESYAGHYIP AFASRVHQGN 

       250        260        270        280        290        300 
KKNEGTHINL KGFAIGNGLT DPAIQYKAYT DYALEMNLIQ KADYERINKF IPPCEFAIKL 

       310        320        330        340        350        360 
CGTNGKASCM AAYMVCNTIF NSIMKLVGTK NYYDVRKECE GKLCYDFSNL EKFFGDKAVR 

       370        380        390        400        410        420 
QAIGVGDIEF VSCSTSVYQA MLTDWMRNLE VGIPALLEDG INVLIYAGEY DLICNWLGNS 

       430        440        450        460        470        480 
RWVHSMEWSG QKDFAKTAES SFLVDDAQAG VLKSHGALSF LKVHNAGHMV PMDQPKAALE 

       490        500 
MLRRFTQGKL KEAVPEEESS TTSFYAAM 

« Hide

References

[1]Rocher A., Lok F., Cameron-Mills V., von Wettstein D.
Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Himalaya.
Tissue: Aleurone.
[2]"Primary structure of carboxypeptidase III from malted barley."
Soerensen S.B., Svendsen I., Breddam K.
Carlsberg Res. Commun. 54:193-202(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 81-491.
Strain: cv. Gula.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y09604 mRNA. Translation: CAA70817.1.
PIRA35275.

3D structure databases

ProteinModelPortalP21529.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS10.009.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneP21529.

Gene expression databases

GenevestigatorP21529.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERPTHR11802. PTHR11802. 1 hit.
PfamPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSPR00724. CRBOXYPTASEC.
SUPFAMSSF53474. SSF53474. 1 hit.
PROSITEPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCBP3_HORVU
AccessionPrimary (citable) accession number: P21529
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: July 15, 1998
Last modified: June 11, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries